α-GALACTOSIDASE ACTIVITY AND CARBOHYDRATE MOBILIZATION IN SEEDS OF Dalbergia nigra (Vell.) Allemão ex Benth. – FABACEAE (BRAZILIAN ROSEWOOD) DURING GERMINATION
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Cerne (Online) |
Texto Completo: | https://cerne.ufla.br/site/index.php/CERNE/article/view/132 |
Resumo: | Complex substances are converted by enzyme action into soluble molecules during the germination process, and these are in turn translocated to the growing plant embryo, serving as an energy source or physical structure. With the objective of quantifying α-galactosidase enzyme activity as well as mono- and oligosaccharide mobilization during germination, this study was conducted using Brazilian rosewood seeds. Seeds were kept in a germinator at 25ºC under continuous light for evaluation of radicle emergence (protrusion) over a period of 10 days. Mono- and oligosaccharide contents and also the specific activity of α–galactosidase enzyme were quantified on days zero, one, three and five. There was mobilization of glucose and mannose stored in cotyledons, and also of xylose in cotyledons and in the embryo in the first three days of germination. Rhamnose contents increased in cotyledons and in the embryonic axis. Raffinose was the initially used oligosaccharide, both in the embryonic axis and in cotyledons, while sucrose accumulated in both. Enzyme activity varied throughout, with greater specific activity on day one of imbibition, both in cotyledons and in the embryonic axis. |
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α-GALACTOSIDASE ACTIVITY AND CARBOHYDRATE MOBILIZATION IN SEEDS OF Dalbergia nigra (Vell.) Allemão ex Benth. – FABACEAE (BRAZILIAN ROSEWOOD) DURING GERMINATIONPhysiologyenzymereservesComplex substances are converted by enzyme action into soluble molecules during the germination process, and these are in turn translocated to the growing plant embryo, serving as an energy source or physical structure. With the objective of quantifying α-galactosidase enzyme activity as well as mono- and oligosaccharide mobilization during germination, this study was conducted using Brazilian rosewood seeds. Seeds were kept in a germinator at 25ºC under continuous light for evaluation of radicle emergence (protrusion) over a period of 10 days. Mono- and oligosaccharide contents and also the specific activity of α–galactosidase enzyme were quantified on days zero, one, three and five. There was mobilization of glucose and mannose stored in cotyledons, and also of xylose in cotyledons and in the embryo in the first three days of germination. Rhamnose contents increased in cotyledons and in the embryonic axis. Raffinose was the initially used oligosaccharide, both in the embryonic axis and in cotyledons, while sucrose accumulated in both. Enzyme activity varied throughout, with greater specific activity on day one of imbibition, both in cotyledons and in the embryonic axis.CERNECERNE2015-05-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://cerne.ufla.br/site/index.php/CERNE/article/view/132CERNE; Vol. 16 No. 3 (2010); 283-289CERNE; v. 16 n. 3 (2010); 283-2892317-63420104-7760reponame:Cerne (Online)instname:Universidade Federal de Lavras (UFLA)instacron:UFLAporhttps://cerne.ufla.br/site/index.php/CERNE/article/view/132/105Copyright (c) 2015 Lanna Clicia Carrijo, Eduardo Euclydes de Lima e Borges, Claudia Aparecida Pontes, Mariana Rocha Lopes, Arno Bruneinfo:eu-repo/semantics/openAccessCarrijo, Lanna CliciaBorges, Eduardo Euclydes de Lima ePontes, Claudia AparecidaLopes, Mariana RochaBrune, Arno2015-05-14T12:07:47Zoai:cerne.ufla.br:article/132Revistahttps://cerne.ufla.br/site/index.php/CERNEPUBhttps://cerne.ufla.br/site/index.php/CERNE/oaicerne@dcf.ufla.br||cerne@dcf.ufla.br2317-63420104-7760opendoar:2024-05-21T19:53:32.685440Cerne (Online) - Universidade Federal de Lavras (UFLA)true |
dc.title.none.fl_str_mv |
α-GALACTOSIDASE ACTIVITY AND CARBOHYDRATE MOBILIZATION IN SEEDS OF Dalbergia nigra (Vell.) Allemão ex Benth. – FABACEAE (BRAZILIAN ROSEWOOD) DURING GERMINATION |
title |
α-GALACTOSIDASE ACTIVITY AND CARBOHYDRATE MOBILIZATION IN SEEDS OF Dalbergia nigra (Vell.) Allemão ex Benth. – FABACEAE (BRAZILIAN ROSEWOOD) DURING GERMINATION |
spellingShingle |
α-GALACTOSIDASE ACTIVITY AND CARBOHYDRATE MOBILIZATION IN SEEDS OF Dalbergia nigra (Vell.) Allemão ex Benth. – FABACEAE (BRAZILIAN ROSEWOOD) DURING GERMINATION Carrijo, Lanna Clicia Physiology enzyme reserves |
title_short |
α-GALACTOSIDASE ACTIVITY AND CARBOHYDRATE MOBILIZATION IN SEEDS OF Dalbergia nigra (Vell.) Allemão ex Benth. – FABACEAE (BRAZILIAN ROSEWOOD) DURING GERMINATION |
title_full |
α-GALACTOSIDASE ACTIVITY AND CARBOHYDRATE MOBILIZATION IN SEEDS OF Dalbergia nigra (Vell.) Allemão ex Benth. – FABACEAE (BRAZILIAN ROSEWOOD) DURING GERMINATION |
title_fullStr |
α-GALACTOSIDASE ACTIVITY AND CARBOHYDRATE MOBILIZATION IN SEEDS OF Dalbergia nigra (Vell.) Allemão ex Benth. – FABACEAE (BRAZILIAN ROSEWOOD) DURING GERMINATION |
title_full_unstemmed |
α-GALACTOSIDASE ACTIVITY AND CARBOHYDRATE MOBILIZATION IN SEEDS OF Dalbergia nigra (Vell.) Allemão ex Benth. – FABACEAE (BRAZILIAN ROSEWOOD) DURING GERMINATION |
title_sort |
α-GALACTOSIDASE ACTIVITY AND CARBOHYDRATE MOBILIZATION IN SEEDS OF Dalbergia nigra (Vell.) Allemão ex Benth. – FABACEAE (BRAZILIAN ROSEWOOD) DURING GERMINATION |
author |
Carrijo, Lanna Clicia |
author_facet |
Carrijo, Lanna Clicia Borges, Eduardo Euclydes de Lima e Pontes, Claudia Aparecida Lopes, Mariana Rocha Brune, Arno |
author_role |
author |
author2 |
Borges, Eduardo Euclydes de Lima e Pontes, Claudia Aparecida Lopes, Mariana Rocha Brune, Arno |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Carrijo, Lanna Clicia Borges, Eduardo Euclydes de Lima e Pontes, Claudia Aparecida Lopes, Mariana Rocha Brune, Arno |
dc.subject.por.fl_str_mv |
Physiology enzyme reserves |
topic |
Physiology enzyme reserves |
description |
Complex substances are converted by enzyme action into soluble molecules during the germination process, and these are in turn translocated to the growing plant embryo, serving as an energy source or physical structure. With the objective of quantifying α-galactosidase enzyme activity as well as mono- and oligosaccharide mobilization during germination, this study was conducted using Brazilian rosewood seeds. Seeds were kept in a germinator at 25ºC under continuous light for evaluation of radicle emergence (protrusion) over a period of 10 days. Mono- and oligosaccharide contents and also the specific activity of α–galactosidase enzyme were quantified on days zero, one, three and five. There was mobilization of glucose and mannose stored in cotyledons, and also of xylose in cotyledons and in the embryo in the first three days of germination. Rhamnose contents increased in cotyledons and in the embryonic axis. Raffinose was the initially used oligosaccharide, both in the embryonic axis and in cotyledons, while sucrose accumulated in both. Enzyme activity varied throughout, with greater specific activity on day one of imbibition, both in cotyledons and in the embryonic axis. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-05-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://cerne.ufla.br/site/index.php/CERNE/article/view/132 |
url |
https://cerne.ufla.br/site/index.php/CERNE/article/view/132 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://cerne.ufla.br/site/index.php/CERNE/article/view/132/105 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
CERNE CERNE |
publisher.none.fl_str_mv |
CERNE CERNE |
dc.source.none.fl_str_mv |
CERNE; Vol. 16 No. 3 (2010); 283-289 CERNE; v. 16 n. 3 (2010); 283-289 2317-6342 0104-7760 reponame:Cerne (Online) instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Cerne (Online) |
collection |
Cerne (Online) |
repository.name.fl_str_mv |
Cerne (Online) - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
cerne@dcf.ufla.br||cerne@dcf.ufla.br |
_version_ |
1799874939231141888 |