GENETIC CHARACTERIZATION OF REMAINING POPULATIONS OF Paratecoma peroba, AN ENDANGERED AND ENDEMIC SPECIES OF THE ATLANTIC FOREST

• Autor(a) principal: Cerri França , Tábatta Caroline
• Data de Publicação: 2022
• Outros Autores: Rigo Tavares , Leticia, Silva Júnior, Adelson Lemes da, Demolinari de Miranda, Fábio, Batista Vargas , Lucas, Maria Pedra de Abreu , Karla, Winckler Caldeira , Marcos Vinicius
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Cerne (Online)
• Texto Completo: https://cerne.ufla.br/site/index.php/CERNE/article/view/3055
• topic: Forest conservation; Genetic diversity; ISSR; Peroba do campo; Population structure; Conservação florestal; Diversidade genética; ISSR; Peroba do campo; Estrutura populacional

Background: The objective of this study was the genetic characterization of remaining populations ofthe species Paratecoma peroba occurring in fragments of the Atlantic Forest, by estimating parametersof diversity and genetic structure. The study was carried out in two forest fragments, the Atlantic ForestEnvironmental Education Pole (area 1) and the Pacotuba National Forest (area 2), where 93 adult treeswere identified.Results: Ten Inter Simple Sequence Repeats (ISSR) primers were used in genotyping, obtaining 112amplified bands with 87.5% polymorphism. The genetic diversity estimated for the populations from theNei (H*) and Shannon (I*) indices was higher for area 1. For the joint data, moderate genetic diversitywas observed, referring to the average values of 0.26 and 0.40, obtained for the H* and I* indices,respectively. Molecular variance analysis resulted in moderate differentiation between populations (ΦST= 0.143), while gene flow analysis (N m = 6.69) revealed the occurrence of similar alleles between them.However, the predominance of a single genetic group in area 2, revealed from Bayesian approach,indicates that its genetic structure, possibly generated by the current scenario of fragmentation of theAtlantic Forest and the distancing of populations, affecting the contemporary gene flow.Conclusion: Despite the moderate genetic diversity of the species for the area 2 population, actionstoward inclusion of seedlings obtained from seeds from neighboring fragments, including area 1, and theincrease in the connectivity of forest fragments through ecological corridors, could assist in augmentingits genetic variation.