Methodology for biological studies of mealybugs (Hemiptera: Pseudococcidae)

Detalhes bibliográficos
Autor(a) principal: Santa-Cecília, Lenira Viana Costa
Data de Publicação: 2008
Outros Autores: Prado, Ernesto, Borges, Cristina Machado, Correa, Lílian Roberta Batista, Souza, Brígida
Tipo de documento: Artigo
Idioma: por
eng
Título da fonte: Coffee Science (Online)
Texto Completo: https://coffeescience.ufla.br/index.php/Coffeescience/article/view/86
Resumo: Several methodologies have been used in biological studies of mealybugs (Pseudococcidae) in the laboratory. The objective of this work was to compare three methodologies in order to establish a pattern for development studies. The development nymphal period and mortality of the citrus mealybug, Planococcus citri (Risso, 1813), was evaluated in PVC clip cages of 10 and 30 mm in diameter attached to leaves of coffee plants (Coffea arabica L. cv. Acaia Cerrado), and on leaf sections placed over an agar film layer. Forty mealybug eggs were individually placed on the substrate and evaluated daily. The data were submitted to analysis of variance followed by the Test of Tukey (p≤ 0.005). Differences were detected in citrus mealybug nymphal development period and mortality depending on the used methodology. The shortest period and the lowest mortality were obtained using foliar sections maintained in agar-water which appears to be a viable methodology for mealybug studies in the laboratory. The excessive insect manipulation seems to be the main negative factor in mealybug development when using clip cages fixed to plant leaves.
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spelling Methodology for biological studies of mealybugs (Hemiptera: Pseudococcidae)Metodologia para estudos biológicos de cochonilhas (Hemiptera: Pseudococcidae)Coccoideadevelopmentbiologyclip cagesleaf sectionsCoccoideacochonilhas-farinhentasbiologiagaiolasecções foliaresSeveral methodologies have been used in biological studies of mealybugs (Pseudococcidae) in the laboratory. The objective of this work was to compare three methodologies in order to establish a pattern for development studies. The development nymphal period and mortality of the citrus mealybug, Planococcus citri (Risso, 1813), was evaluated in PVC clip cages of 10 and 30 mm in diameter attached to leaves of coffee plants (Coffea arabica L. cv. Acaia Cerrado), and on leaf sections placed over an agar film layer. Forty mealybug eggs were individually placed on the substrate and evaluated daily. The data were submitted to analysis of variance followed by the Test of Tukey (p≤ 0.005). Differences were detected in citrus mealybug nymphal development period and mortality depending on the used methodology. The shortest period and the lowest mortality were obtained using foliar sections maintained in agar-water which appears to be a viable methodology for mealybug studies in the laboratory. The excessive insect manipulation seems to be the main negative factor in mealybug development when using clip cages fixed to plant leaves.Várias metodologias têm sido empregadas em estudos de biologia de cochonilhas (Pseudococcidae) em laboratório e objetivou-se neste trabalho comparar três metodologias visando ao estabelecimento de um padrão. O desenvolvimento ninfal e a mortalidade da cochonilha Planococus citri (Risso, 1813) foram avaliados em gaiolas de PVC transparente de 10 mm e 30 mm de diâmetro fixadas em folhas de cafeeiro (Coffea arabica L. cv. Acaia Cerrado), e em secções foliares mantidas em ágar-água. Ovos da cochonilha foram individualizados sobre o substrato e seu desenvolvimento avaliado diariamente. Os dados foram submetidos à análise de variância, seguidos pelo Teste de Tukey a 5% de significância. Foram constatadas diferenças na duração do período ninfal e na mortalidade da cochonilha em função das três metodologias testadas. A menor duração do desenvolvimento e a menor mortalidade foram obtidas usando secções foliares mantidas em ágar-água, demonstrando que essa é uma metodologia viável para estudos de biologia de cochonilhas-farinhentas em laboratório. A excessiva manipulação do inseto parece ter sido o principal fator negativo no desenvolvimento da cochonilha, quando foram utilizadas gaiolas (10 e 30 mm de diâmetro) fixadas nas folhas das plantas de cafeeiro.Editora UFLA2008-12-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://coffeescience.ufla.br/index.php/Coffeescience/article/view/86Coffee Science - ISSN 1984-3909; Vol. 3 No. 2 (2008); 152-155Coffee Science; Vol. 3 Núm. 2 (2008); 152-155Coffee Science; v. 3 n. 2 (2008); 152-1551984-3909reponame:Coffee Science (Online)instname:Universidade Federal de Lavras (UFLA)instacron:UFLAporenghttps://coffeescience.ufla.br/index.php/Coffeescience/article/view/86/174https://coffeescience.ufla.br/index.php/Coffeescience/article/view/86/175Copyright (c) 2008 Coffee Science - ISSN 1984-3909https://creativecommons.org/info:eu-repo/semantics/openAccessSanta-Cecília, Lenira Viana CostaPrado, ErnestoBorges, Cristina MachadoCorrea, Lílian Roberta BatistaSouza, Brígida2013-02-23T12:54:18Zoai:coffeescience.ufla.br:article/86Revistahttps://coffeescience.ufla.br/index.php/CoffeesciencePUBhttps://coffeescience.ufla.br/index.php/Coffeescience/oaicoffeescience@dag.ufla.br||coffeescience@dag.ufla.br|| alvaro-cozadi@hotmail.com1984-39091809-6875opendoar:2024-05-21T19:53:30.410819Coffee Science (Online) - Universidade Federal de Lavras (UFLA)true
dc.title.none.fl_str_mv Methodology for biological studies of mealybugs (Hemiptera: Pseudococcidae)
Metodologia para estudos biológicos de cochonilhas (Hemiptera: Pseudococcidae)
title Methodology for biological studies of mealybugs (Hemiptera: Pseudococcidae)
spellingShingle Methodology for biological studies of mealybugs (Hemiptera: Pseudococcidae)
Santa-Cecília, Lenira Viana Costa
Coccoidea
development
biology
clip cages
leaf sections
Coccoidea
cochonilhas-farinhentas
biologia
gaiola
secções foliares
title_short Methodology for biological studies of mealybugs (Hemiptera: Pseudococcidae)
title_full Methodology for biological studies of mealybugs (Hemiptera: Pseudococcidae)
title_fullStr Methodology for biological studies of mealybugs (Hemiptera: Pseudococcidae)
title_full_unstemmed Methodology for biological studies of mealybugs (Hemiptera: Pseudococcidae)
title_sort Methodology for biological studies of mealybugs (Hemiptera: Pseudococcidae)
author Santa-Cecília, Lenira Viana Costa
author_facet Santa-Cecília, Lenira Viana Costa
Prado, Ernesto
Borges, Cristina Machado
Correa, Lílian Roberta Batista
Souza, Brígida
author_role author
author2 Prado, Ernesto
Borges, Cristina Machado
Correa, Lílian Roberta Batista
Souza, Brígida
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Santa-Cecília, Lenira Viana Costa
Prado, Ernesto
Borges, Cristina Machado
Correa, Lílian Roberta Batista
Souza, Brígida
dc.subject.por.fl_str_mv Coccoidea
development
biology
clip cages
leaf sections
Coccoidea
cochonilhas-farinhentas
biologia
gaiola
secções foliares
topic Coccoidea
development
biology
clip cages
leaf sections
Coccoidea
cochonilhas-farinhentas
biologia
gaiola
secções foliares
description Several methodologies have been used in biological studies of mealybugs (Pseudococcidae) in the laboratory. The objective of this work was to compare three methodologies in order to establish a pattern for development studies. The development nymphal period and mortality of the citrus mealybug, Planococcus citri (Risso, 1813), was evaluated in PVC clip cages of 10 and 30 mm in diameter attached to leaves of coffee plants (Coffea arabica L. cv. Acaia Cerrado), and on leaf sections placed over an agar film layer. Forty mealybug eggs were individually placed on the substrate and evaluated daily. The data were submitted to analysis of variance followed by the Test of Tukey (p≤ 0.005). Differences were detected in citrus mealybug nymphal development period and mortality depending on the used methodology. The shortest period and the lowest mortality were obtained using foliar sections maintained in agar-water which appears to be a viable methodology for mealybug studies in the laboratory. The excessive insect manipulation seems to be the main negative factor in mealybug development when using clip cages fixed to plant leaves.
publishDate 2008
dc.date.none.fl_str_mv 2008-12-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://coffeescience.ufla.br/index.php/Coffeescience/article/view/86
url https://coffeescience.ufla.br/index.php/Coffeescience/article/view/86
dc.language.iso.fl_str_mv por
eng
language por
eng
dc.relation.none.fl_str_mv https://coffeescience.ufla.br/index.php/Coffeescience/article/view/86/174
https://coffeescience.ufla.br/index.php/Coffeescience/article/view/86/175
dc.rights.driver.fl_str_mv Copyright (c) 2008 Coffee Science - ISSN 1984-3909
https://creativecommons.org/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2008 Coffee Science - ISSN 1984-3909
https://creativecommons.org/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Editora UFLA
publisher.none.fl_str_mv Editora UFLA
dc.source.none.fl_str_mv Coffee Science - ISSN 1984-3909; Vol. 3 No. 2 (2008); 152-155
Coffee Science; Vol. 3 Núm. 2 (2008); 152-155
Coffee Science; v. 3 n. 2 (2008); 152-155
1984-3909
reponame:Coffee Science (Online)
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Coffee Science (Online)
collection Coffee Science (Online)
repository.name.fl_str_mv Coffee Science (Online) - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv coffeescience@dag.ufla.br||coffeescience@dag.ufla.br|| alvaro-cozadi@hotmail.com
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