Long term conservation of embryonic axes of genipap accessions
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/46788 |
Resumo: | The ex situ conservation of seeds in cryobanks demands previous studies of dehydration procedures for the adjustment of the adequate moisture that makes long-term conservation possible. The objective of this work was to evaluate the effect of dehydration time on the regenerative capacity of embryonic axes of two genipap accessions (Umbaúba and Núcleo Bandeirante). Seeds were submitted to desiccation in policarbonate box containing silica gel for 0, 12, 16 and 20 h at room temperature and after each period, seed moisture content was determined. Seeds were inoculated in germination medium and samples were inserted into cryotubes and immersed in liquid nitrogen (-196ºC). Desiccation in silica gel was efficient in reducing the moisture content in both accessions. Umbaúba accession presented 100% germination at all desiccation treatments before cryopreservation and Núcleo Bandeirante was more sensitive to desiccation with 10% germination in 20 h. Umbaúba accession showed a reduction of growth variables with increased desiccation time. The treatments tested did not affect the growth variables of Núcleo Bandeirante accession. The desiccation, although changes observed in the ultra-structures of embryonic axis, did not influence the regeneration percentage of cryopreserved accessions. |
id |
UFLA_029c0b295674a2d37f4048d81efdc874 |
---|---|
oai_identifier_str |
oai:localhost:1/46788 |
network_acronym_str |
UFLA |
network_name_str |
Repositório Institucional da UFLA |
repository_id_str |
|
spelling |
Long term conservation of embryonic axes of genipap accessionsConservação a longo prazo de eixos embrionários de acessos de jenipapeiroGenipa americana L.SeedTissue cultureCryopreservationRegenerationSementesCultura de tecidosCriopreservaçãoDessecaçãoThe ex situ conservation of seeds in cryobanks demands previous studies of dehydration procedures for the adjustment of the adequate moisture that makes long-term conservation possible. The objective of this work was to evaluate the effect of dehydration time on the regenerative capacity of embryonic axes of two genipap accessions (Umbaúba and Núcleo Bandeirante). Seeds were submitted to desiccation in policarbonate box containing silica gel for 0, 12, 16 and 20 h at room temperature and after each period, seed moisture content was determined. Seeds were inoculated in germination medium and samples were inserted into cryotubes and immersed in liquid nitrogen (-196ºC). Desiccation in silica gel was efficient in reducing the moisture content in both accessions. Umbaúba accession presented 100% germination at all desiccation treatments before cryopreservation and Núcleo Bandeirante was more sensitive to desiccation with 10% germination in 20 h. Umbaúba accession showed a reduction of growth variables with increased desiccation time. The treatments tested did not affect the growth variables of Núcleo Bandeirante accession. The desiccation, although changes observed in the ultra-structures of embryonic axis, did not influence the regeneration percentage of cryopreserved accessions.A conservação ex situ de sementes em bancos criogênicos exige estudos prévios de procedimentos de desidratação para o ajuste da umidade adequada que possibilita a conservação em longo prazo. O objetivo deste trabalho foi avaliar o efeito do tempo de desidratação sobre a capacidade regenerativa de eixos embrionários de dois acessos de jenipapo, Umbaúba e Núcleo Bandeirante. As sementes foram submetidas à dessecação em caixas de policarbonato contendo sílica gel por 0, 12, 16 e 20 h em temperatura ambiente e, após cada período, o teor de umidade da semente foi determinado. As sementes foram inoculadas no meio de germinação e amostras para cada período foram inseridas em criotubos e imersas em nitrogênio líquido (- 196ºC). A dessecação em sílica gel foi eficiente na redução do teor de umidade (% mc) de ambos os acessos. O acesso Umbaúba apresentou 100% de germinação em todos os tempos de dessecação antes da criopreservação e o Núcleo Bandeirante foi mais sensível à dessecação com 10% de germinação em 20 h. O acesso Umbaúba apresentou uma redução das variáveis de crescimento com o aumento do tempo de dessecação. Os tempos testados não afetaram as variáveis de crescimento do acesso Núcleo Bandeirante. O tempo de dessecação, apesar das alterações nas ultraestruturas do eixo embrionário, não influenciou a porcentagem de regeneração dos acessos criopreservados.Scientia Plena2021-07-20T17:45:14Z2021-07-20T17:45:14Z2020-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfNASCIMENTO, C. M. do et al. Long term conservation of embryonic axes of genipap accessions. Scientia Plena, [S. I.], v. 16, n. 2, fev. 2020. DOI: 10.14808/sci.plena.2020.020201.http://repositorio.ufla.br/jspui/handle/1/46788Scientia Plenareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessNascimento, Cyntia Maia doOliveira, Leila Albuquerque Resende deSilva, Ana Veruska Cruz daCastro, Evaristo Mauro deLédo, Ana da Silvaeng2021-07-20T17:46:28Zoai:localhost:1/46788Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2021-07-20T17:46:28Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Long term conservation of embryonic axes of genipap accessions Conservação a longo prazo de eixos embrionários de acessos de jenipapeiro |
title |
Long term conservation of embryonic axes of genipap accessions |
spellingShingle |
Long term conservation of embryonic axes of genipap accessions Nascimento, Cyntia Maia do Genipa americana L. Seed Tissue culture Cryopreservation Regeneration Sementes Cultura de tecidos Criopreservação Dessecação |
title_short |
Long term conservation of embryonic axes of genipap accessions |
title_full |
Long term conservation of embryonic axes of genipap accessions |
title_fullStr |
Long term conservation of embryonic axes of genipap accessions |
title_full_unstemmed |
Long term conservation of embryonic axes of genipap accessions |
title_sort |
Long term conservation of embryonic axes of genipap accessions |
author |
Nascimento, Cyntia Maia do |
author_facet |
Nascimento, Cyntia Maia do Oliveira, Leila Albuquerque Resende de Silva, Ana Veruska Cruz da Castro, Evaristo Mauro de Lédo, Ana da Silva |
author_role |
author |
author2 |
Oliveira, Leila Albuquerque Resende de Silva, Ana Veruska Cruz da Castro, Evaristo Mauro de Lédo, Ana da Silva |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Nascimento, Cyntia Maia do Oliveira, Leila Albuquerque Resende de Silva, Ana Veruska Cruz da Castro, Evaristo Mauro de Lédo, Ana da Silva |
dc.subject.por.fl_str_mv |
Genipa americana L. Seed Tissue culture Cryopreservation Regeneration Sementes Cultura de tecidos Criopreservação Dessecação |
topic |
Genipa americana L. Seed Tissue culture Cryopreservation Regeneration Sementes Cultura de tecidos Criopreservação Dessecação |
description |
The ex situ conservation of seeds in cryobanks demands previous studies of dehydration procedures for the adjustment of the adequate moisture that makes long-term conservation possible. The objective of this work was to evaluate the effect of dehydration time on the regenerative capacity of embryonic axes of two genipap accessions (Umbaúba and Núcleo Bandeirante). Seeds were submitted to desiccation in policarbonate box containing silica gel for 0, 12, 16 and 20 h at room temperature and after each period, seed moisture content was determined. Seeds were inoculated in germination medium and samples were inserted into cryotubes and immersed in liquid nitrogen (-196ºC). Desiccation in silica gel was efficient in reducing the moisture content in both accessions. Umbaúba accession presented 100% germination at all desiccation treatments before cryopreservation and Núcleo Bandeirante was more sensitive to desiccation with 10% germination in 20 h. Umbaúba accession showed a reduction of growth variables with increased desiccation time. The treatments tested did not affect the growth variables of Núcleo Bandeirante accession. The desiccation, although changes observed in the ultra-structures of embryonic axis, did not influence the regeneration percentage of cryopreserved accessions. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-03 2021-07-20T17:45:14Z 2021-07-20T17:45:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
NASCIMENTO, C. M. do et al. Long term conservation of embryonic axes of genipap accessions. Scientia Plena, [S. I.], v. 16, n. 2, fev. 2020. DOI: 10.14808/sci.plena.2020.020201. http://repositorio.ufla.br/jspui/handle/1/46788 |
identifier_str_mv |
NASCIMENTO, C. M. do et al. Long term conservation of embryonic axes of genipap accessions. Scientia Plena, [S. I.], v. 16, n. 2, fev. 2020. DOI: 10.14808/sci.plena.2020.020201. |
url |
http://repositorio.ufla.br/jspui/handle/1/46788 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Scientia Plena |
publisher.none.fl_str_mv |
Scientia Plena |
dc.source.none.fl_str_mv |
Scientia Plena reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835204685922304 |