Infecção por Mycobacterium sp. em herbívoros selvagens de cativeiro no Rio Grande do Sul: estudo retrospectivo e detecção imuno-histoquímica (2003-2015)

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Vanessa L.
Data de Publicação: 2017
Outros Autores: Souza, Suyene O., Casagrande, Renata A., Wouters, Angelica T. B., Wouters, Flademir, Rolim, Veronica M., Santos, Elisandro O., Driemeier, David
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/29856
Resumo: Tuberculosis is a debilitating infecto-contagious disease, caused by an acid-fast bacillus (AFB) that belong to different species of the Mycobacterium tuberculosis complex (MTC). Mycobacteriosis are important diseases in veterinary medicine because of their zoonotic potential and worldwide distribution, affecting all classes of vertebrates. In wild animals the mycobacteriosis have been reported mainly as a problem in captivity. There are also reports in free-ranging wildlife, endangering and hampering tuberculosis erradication programs in animal production. The diagnosis of the disease in wildlife is usually postmortem, because the tuberculin test is not standardized for wildlife species, and also it is not reliable for screening. The postmortem diagnosis is based on macroscopic findings, microscopic detection of AFB at Ziehl-Neelsen staining (ZN), and mainly isolation and identification of the agent. However, only gross and microscopic exams do not allow to distinguish the species of Mycobacterium involved. The immunostaining with polyclonal anti-Mycobacterium tuberculosis confirms tuberculosis infection, but is not specific; there may be marking of other mycobacteria. The aim of this study was to describe the histologic findings, of ZN staining and immunohistochemistry technique (IHC) of 13 cases of wildlife herbivores diagnosed with tuberculosis in the Setor de Patologia Veterinária of the Universidade Federal do Rio Grande do Sul (SPV-UFRGS) during the 2003- 2015 period. Formalin fixed paraffin embedded tissues were recut, stained with hematoxylin and eosin and ZN, and samples were submitted to the IHC (anti-M. tuberculosis marking, streptavidin-biotin peroxidase method). All animals included were adults living in captivity and belonged to the following species: llama (5/13), sambar deer (4/13), camel (1/13), red deer (1/13), Brazilian tapir (1/13) and Nilgai antelope (1/13). The IHC revealed immunostaining of accentuated (4/13), moderate (4/13) and discrete (4/13) intensity, except in a case with insufficient material. The histological features, findings in ZN staining and IHC in the wild herbivores with tuberculosis lesions allowed the diagnosis of infection with Mycobacterium sp., turning into fast and efficient methods of diagnosis, which can help to prevent the spread of this disease in animals from the same herd.
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spelling Infecção por Mycobacterium sp. em herbívoros selvagens de cativeiro no Rio Grande do Sul: estudo retrospectivo e detecção imuno-histoquímica (2003-2015)Mycobacterium sp.TuberculosisHerbivoresCamelidsDeerHistopathologyImmunohistochemistryDiagnosisTuberculoseHerbívoroCamelídeoCervídeoHistopatologiaImuno-histoquímicaTuberculosis is a debilitating infecto-contagious disease, caused by an acid-fast bacillus (AFB) that belong to different species of the Mycobacterium tuberculosis complex (MTC). Mycobacteriosis are important diseases in veterinary medicine because of their zoonotic potential and worldwide distribution, affecting all classes of vertebrates. In wild animals the mycobacteriosis have been reported mainly as a problem in captivity. There are also reports in free-ranging wildlife, endangering and hampering tuberculosis erradication programs in animal production. The diagnosis of the disease in wildlife is usually postmortem, because the tuberculin test is not standardized for wildlife species, and also it is not reliable for screening. The postmortem diagnosis is based on macroscopic findings, microscopic detection of AFB at Ziehl-Neelsen staining (ZN), and mainly isolation and identification of the agent. However, only gross and microscopic exams do not allow to distinguish the species of Mycobacterium involved. The immunostaining with polyclonal anti-Mycobacterium tuberculosis confirms tuberculosis infection, but is not specific; there may be marking of other mycobacteria. The aim of this study was to describe the histologic findings, of ZN staining and immunohistochemistry technique (IHC) of 13 cases of wildlife herbivores diagnosed with tuberculosis in the Setor de Patologia Veterinária of the Universidade Federal do Rio Grande do Sul (SPV-UFRGS) during the 2003- 2015 period. Formalin fixed paraffin embedded tissues were recut, stained with hematoxylin and eosin and ZN, and samples were submitted to the IHC (anti-M. tuberculosis marking, streptavidin-biotin peroxidase method). All animals included were adults living in captivity and belonged to the following species: llama (5/13), sambar deer (4/13), camel (1/13), red deer (1/13), Brazilian tapir (1/13) and Nilgai antelope (1/13). The IHC revealed immunostaining of accentuated (4/13), moderate (4/13) and discrete (4/13) intensity, except in a case with insufficient material. The histological features, findings in ZN staining and IHC in the wild herbivores with tuberculosis lesions allowed the diagnosis of infection with Mycobacterium sp., turning into fast and efficient methods of diagnosis, which can help to prevent the spread of this disease in animals from the same herd.A tuberculose é uma enfermidade infectocontagiosa, debilitante, causada por bacilos álcool-ácido resistentes (BAAR), pertencentes ao complexo Mycobacterium tuberculosis (CMT). As micobacterioses têm importância em Medicina Veterinária devido ao seu potencial zoonótico e sua distribuição mundial, afetando todas as classes de vertebrados. Em animais selvagens as micobacterioses têm sido um problema relatado principalmente em cativeiro. Contudo, há relatos de sua ocorrência também em animais de vida livre, colocando em risco e dificultando os programas de erradicação da tuberculose em animais de produção. O diagnóstico nas espécies selvagens em geral é post mortem, uma vez que o teste de tuberculina não está padronizado para essas espécies, assim como não é confiável para triagem. São consideradas para o diagnóstico lesões de necropsia, observação microscópica de BAAR na coloração de Ziehl-Neelsen (ZN) e, principalmente, isolamento e identificação do agente. No entanto, apenas os achados morfológicos macro e microscópicos não permitem distinguir a espécie de Mycobacterium envolvida. A técnica de imuno-histoquímica (IHQ) com anticorpo policlonal anti- -M. tuberculosis confirma a infecção pelo CMT, mas não é específica, pois pode ocorrer marcação de outras micobactérias. As características histológicas, os achados na coloração de ZN e na IHQ de 13 casos de herbívoros selvagens diagnosticados com tuberculose no Setor de Patologia Veterinária da Universidade Federal do Rio Grande do Sul (SPV-UFRGS) no período de 2003 a 2015 são descritos. A partir das amostras em blocos de parafina foram confeccionadas novas lâminas histológicas, coradas com hematoxilina e eosina (HE) e ZN. Cortes foram submetidos à técnica de IHQ para detecção do CMT. Todos os animais eram adultos, provenientes de cativeiro e incluíram lhama (5/13), cervo sambar (4/13), camelo (1/13), cervo vermelho (1/13), anta brasileira (1/13) e antílope Nilgai (1/13). Na IHQ observou-se imunomarcação acentuada (4/13), moderada (4/13) ou discreta (4/13), exceto em um caso, em que não havia quantidade suficiente de material. As características histológicas, bem como os achados na coloração de ZN e na técnica de IHQ confirmaram o diagnóstico de infecção por Mycobacterium sp. e foram considerados métodos rápidos e eficientes, de forma que podem ajudar na prevenção da disseminação da doença em animais.Colégio Brasileiro de Patologia Animal (CBPA) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)2018-08-01T19:21:22Z2018-08-01T19:21:22Z2017-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfRIBEIRO, V. L. et al. Infecção por mycobacterium sp. em herbívoros selvagens de cativeiro no Rio Grande do Sul: estudo retrospectivo e detecção imuno-histoquímica (2003-2015). Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 1, p. 58-65, jan. 2017.http://repositorio.ufla.br/jspui/handle/1/29856Pesquisa Veterinária Brasileirareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessRibeiro, Vanessa L.Souza, Suyene O.Casagrande, Renata A.Wouters, Angelica T. B.Wouters, FlademirRolim, Veronica M.Santos, Elisandro O.Driemeier, Davidpor2018-08-01T19:21:22Zoai:localhost:1/29856Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2018-08-01T19:21:22Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Infecção por Mycobacterium sp. em herbívoros selvagens de cativeiro no Rio Grande do Sul: estudo retrospectivo e detecção imuno-histoquímica (2003-2015)
title Infecção por Mycobacterium sp. em herbívoros selvagens de cativeiro no Rio Grande do Sul: estudo retrospectivo e detecção imuno-histoquímica (2003-2015)
spellingShingle Infecção por Mycobacterium sp. em herbívoros selvagens de cativeiro no Rio Grande do Sul: estudo retrospectivo e detecção imuno-histoquímica (2003-2015)
Ribeiro, Vanessa L.
Mycobacterium sp.
Tuberculosis
Herbivores
Camelids
Deer
Histopathology
Immunohistochemistry
Diagnosis
Tuberculose
Herbívoro
Camelídeo
Cervídeo
Histopatologia
Imuno-histoquímica
title_short Infecção por Mycobacterium sp. em herbívoros selvagens de cativeiro no Rio Grande do Sul: estudo retrospectivo e detecção imuno-histoquímica (2003-2015)
title_full Infecção por Mycobacterium sp. em herbívoros selvagens de cativeiro no Rio Grande do Sul: estudo retrospectivo e detecção imuno-histoquímica (2003-2015)
title_fullStr Infecção por Mycobacterium sp. em herbívoros selvagens de cativeiro no Rio Grande do Sul: estudo retrospectivo e detecção imuno-histoquímica (2003-2015)
title_full_unstemmed Infecção por Mycobacterium sp. em herbívoros selvagens de cativeiro no Rio Grande do Sul: estudo retrospectivo e detecção imuno-histoquímica (2003-2015)
title_sort Infecção por Mycobacterium sp. em herbívoros selvagens de cativeiro no Rio Grande do Sul: estudo retrospectivo e detecção imuno-histoquímica (2003-2015)
author Ribeiro, Vanessa L.
author_facet Ribeiro, Vanessa L.
Souza, Suyene O.
Casagrande, Renata A.
Wouters, Angelica T. B.
Wouters, Flademir
Rolim, Veronica M.
Santos, Elisandro O.
Driemeier, David
author_role author
author2 Souza, Suyene O.
Casagrande, Renata A.
Wouters, Angelica T. B.
Wouters, Flademir
Rolim, Veronica M.
Santos, Elisandro O.
Driemeier, David
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ribeiro, Vanessa L.
Souza, Suyene O.
Casagrande, Renata A.
Wouters, Angelica T. B.
Wouters, Flademir
Rolim, Veronica M.
Santos, Elisandro O.
Driemeier, David
dc.subject.por.fl_str_mv Mycobacterium sp.
Tuberculosis
Herbivores
Camelids
Deer
Histopathology
Immunohistochemistry
Diagnosis
Tuberculose
Herbívoro
Camelídeo
Cervídeo
Histopatologia
Imuno-histoquímica
topic Mycobacterium sp.
Tuberculosis
Herbivores
Camelids
Deer
Histopathology
Immunohistochemistry
Diagnosis
Tuberculose
Herbívoro
Camelídeo
Cervídeo
Histopatologia
Imuno-histoquímica
description Tuberculosis is a debilitating infecto-contagious disease, caused by an acid-fast bacillus (AFB) that belong to different species of the Mycobacterium tuberculosis complex (MTC). Mycobacteriosis are important diseases in veterinary medicine because of their zoonotic potential and worldwide distribution, affecting all classes of vertebrates. In wild animals the mycobacteriosis have been reported mainly as a problem in captivity. There are also reports in free-ranging wildlife, endangering and hampering tuberculosis erradication programs in animal production. The diagnosis of the disease in wildlife is usually postmortem, because the tuberculin test is not standardized for wildlife species, and also it is not reliable for screening. The postmortem diagnosis is based on macroscopic findings, microscopic detection of AFB at Ziehl-Neelsen staining (ZN), and mainly isolation and identification of the agent. However, only gross and microscopic exams do not allow to distinguish the species of Mycobacterium involved. The immunostaining with polyclonal anti-Mycobacterium tuberculosis confirms tuberculosis infection, but is not specific; there may be marking of other mycobacteria. The aim of this study was to describe the histologic findings, of ZN staining and immunohistochemistry technique (IHC) of 13 cases of wildlife herbivores diagnosed with tuberculosis in the Setor de Patologia Veterinária of the Universidade Federal do Rio Grande do Sul (SPV-UFRGS) during the 2003- 2015 period. Formalin fixed paraffin embedded tissues were recut, stained with hematoxylin and eosin and ZN, and samples were submitted to the IHC (anti-M. tuberculosis marking, streptavidin-biotin peroxidase method). All animals included were adults living in captivity and belonged to the following species: llama (5/13), sambar deer (4/13), camel (1/13), red deer (1/13), Brazilian tapir (1/13) and Nilgai antelope (1/13). The IHC revealed immunostaining of accentuated (4/13), moderate (4/13) and discrete (4/13) intensity, except in a case with insufficient material. The histological features, findings in ZN staining and IHC in the wild herbivores with tuberculosis lesions allowed the diagnosis of infection with Mycobacterium sp., turning into fast and efficient methods of diagnosis, which can help to prevent the spread of this disease in animals from the same herd.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
2018-08-01T19:21:22Z
2018-08-01T19:21:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv RIBEIRO, V. L. et al. Infecção por mycobacterium sp. em herbívoros selvagens de cativeiro no Rio Grande do Sul: estudo retrospectivo e detecção imuno-histoquímica (2003-2015). Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 1, p. 58-65, jan. 2017.
http://repositorio.ufla.br/jspui/handle/1/29856
identifier_str_mv RIBEIRO, V. L. et al. Infecção por mycobacterium sp. em herbívoros selvagens de cativeiro no Rio Grande do Sul: estudo retrospectivo e detecção imuno-histoquímica (2003-2015). Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 1, p. 58-65, jan. 2017.
url http://repositorio.ufla.br/jspui/handle/1/29856
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dc.rights.driver.fl_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
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rights_invalid_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal (CBPA) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal (CBPA) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira
reponame:Repositório Institucional da UFLA
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