Estabelecimento in vitro de Peltophorum dubium ((Spreng.) Taub.) em função das concentrações do meio MS

Detalhes bibliográficos
Autor(a) principal: Flôres, Andressa Vasconcelos
Data de Publicação: 2015
Outros Autores: Reiniger, Lia Rejane Silveira, Curti, Aline Ritter, Paim, Aline, Bassan, Josiana Scherer, Cunha, Ana Catarina Monteiro Carvalho Mori da
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/14289
Resumo: The objective of this study was to evaluate the influence of several concentrations of MS medium on in vitro establishment of Peltophorum dubium shoot apical segments (80-100 mm) isolated from seedlings germinated under aseptic conditions. The salts concentrations 50, 75, 100, 125 and 150% of MS medium, were supplemented with 30 g L-1 sucrose, 100 mg L-1 of myo-inositol, 7 g L-1 agar and pH adjusted to 5.7. The experimental design was completely randomized, with 20 replications, each consisting of a vial containing one explant. The cultures were maintained for 60 days in culture room under 16-hour photoperiod, light intensity of 20 μmol m-2 s-1 and temperature of 25±2°C. There was no effect of different concentrations of MS medium for most variables. High average of in vitro survival (98%) and establishment (98%) were observed. The largest number of leaves (2.8) occurred at a dilution of 75%, decreasing from this point. At the same concentration the highest callus induction was observed, which was decreasing until not be observed under 125% of original composition; at 150% there was, again, the presence of calli. It was not observed rooting of explants during in vitro cultivation. Considering the set of variables and, additionally, that the presence of calli is not desirable, it is recommended the use of the original concentration of salts from the MS nutrient medium for the in vitro establishment of P. dubium shoot apicals segments.
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spelling Estabelecimento in vitro de Peltophorum dubium ((Spreng.) Taub.) em função das concentrações do meio MSIn vitro establishment of Peltophorum dubium ((Spreng.) Taub.) in function of concentrations of the MS mediumCultura de tecidosSegmentos apicais caulinaresEspécie florestalTissue cultureShoot apical segmentsForestry speciesThe objective of this study was to evaluate the influence of several concentrations of MS medium on in vitro establishment of Peltophorum dubium shoot apical segments (80-100 mm) isolated from seedlings germinated under aseptic conditions. The salts concentrations 50, 75, 100, 125 and 150% of MS medium, were supplemented with 30 g L-1 sucrose, 100 mg L-1 of myo-inositol, 7 g L-1 agar and pH adjusted to 5.7. The experimental design was completely randomized, with 20 replications, each consisting of a vial containing one explant. The cultures were maintained for 60 days in culture room under 16-hour photoperiod, light intensity of 20 μmol m-2 s-1 and temperature of 25±2°C. There was no effect of different concentrations of MS medium for most variables. High average of in vitro survival (98%) and establishment (98%) were observed. The largest number of leaves (2.8) occurred at a dilution of 75%, decreasing from this point. At the same concentration the highest callus induction was observed, which was decreasing until not be observed under 125% of original composition; at 150% there was, again, the presence of calli. It was not observed rooting of explants during in vitro cultivation. Considering the set of variables and, additionally, that the presence of calli is not desirable, it is recommended the use of the original concentration of salts from the MS nutrient medium for the in vitro establishment of P. dubium shoot apicals segments.Neste trabalho, objetivou-se verificar a influência de diferentes concentrações do meio MS no estabelecimento in vitrode segmentos apicais caulinares (80-100 mm) de canafístula isolados, a partir de plântulas germinadas em condições assépticas. As concentrações de sais de 50, 75, 100, 125 e 150% do meio de cultivo MS, foram suplementadas com 30 g L-1 de sacarose, 100 mg L-1 de mio-inositol, 7 g L-1 de ágar e o pH, ajustado para 5,7. O delineamento experimental foi o inteiramente casualizado, com 20 repetições, cada uma consistindo de um frasco contendo um explante. As culturas foram mantidas por 60 dias em sala de cultivo sob fotoperíodo de 16 horas, intensidade luminosa de 20 μmol m-2 s-1 e temperatura de 25±2°C. Não houve efeito das diferentes concentrações do meio MS para a maioria das variáveis, sendo observadas elevadas médias gerais de sobrevivência (98%) e de estabelecimento (98%) in vitro. O maior número de folhas (2,8) ocorreu na diluição de 75%, com redução a partir dessa diluição. Nessa mesma concentração, houve a máxima calogênese, que foi diminuindo até não ser observada sob 125% da composição original; a 150% houve, novamente, presença de calos. Não ocorreu enraizamento de explantes durante o cultivo in vitro. Considerando-se o conjunto das variáveis avaliadas e, adicionalmente, que a presença de calos não é desejável, recomenda-se o emprego da concentração original de sais do meio de cultivo MS no estabelecimento in vitro de segmentos apicais caulinares de canafístulaUniversidade Federal de Lavras (UFLA)2015-05-122017-08-01T20:14:11Z2017-08-01T20:14:11Z2017-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfFLÔRES, A. V. et al. Estabelecimento in vitro de Peltophorum dubium ((Spreng.) Taub) em função das concentrações do meio MS. CERNE, Lavras, v. 17, n. 4, p. 549-553, out./dez. 2011.http://repositorio.ufla.br/jspui/handle/1/142892317-63420104-7760reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAporhttp://www.cerne.ufla.br/site/index.php/CERNE/article/view/87/69Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessFlôres, Andressa VasconcelosReiniger, Lia Rejane SilveiraCurti, Aline RitterPaim, AlineBassan, Josiana SchererCunha, Ana Catarina Monteiro Carvalho Mori da2021-06-01T22:40:38Zoai:localhost:1/14289Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2021-06-01T22:40:38Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Estabelecimento in vitro de Peltophorum dubium ((Spreng.) Taub.) em função das concentrações do meio MS
In vitro establishment of Peltophorum dubium ((Spreng.) Taub.) in function of concentrations of the MS medium
title Estabelecimento in vitro de Peltophorum dubium ((Spreng.) Taub.) em função das concentrações do meio MS
spellingShingle Estabelecimento in vitro de Peltophorum dubium ((Spreng.) Taub.) em função das concentrações do meio MS
Flôres, Andressa Vasconcelos
Cultura de tecidos
Segmentos apicais caulinares
Espécie florestal
Tissue culture
Shoot apical segments
Forestry species
title_short Estabelecimento in vitro de Peltophorum dubium ((Spreng.) Taub.) em função das concentrações do meio MS
title_full Estabelecimento in vitro de Peltophorum dubium ((Spreng.) Taub.) em função das concentrações do meio MS
title_fullStr Estabelecimento in vitro de Peltophorum dubium ((Spreng.) Taub.) em função das concentrações do meio MS
title_full_unstemmed Estabelecimento in vitro de Peltophorum dubium ((Spreng.) Taub.) em função das concentrações do meio MS
title_sort Estabelecimento in vitro de Peltophorum dubium ((Spreng.) Taub.) em função das concentrações do meio MS
author Flôres, Andressa Vasconcelos
author_facet Flôres, Andressa Vasconcelos
Reiniger, Lia Rejane Silveira
Curti, Aline Ritter
Paim, Aline
Bassan, Josiana Scherer
Cunha, Ana Catarina Monteiro Carvalho Mori da
author_role author
author2 Reiniger, Lia Rejane Silveira
Curti, Aline Ritter
Paim, Aline
Bassan, Josiana Scherer
Cunha, Ana Catarina Monteiro Carvalho Mori da
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Flôres, Andressa Vasconcelos
Reiniger, Lia Rejane Silveira
Curti, Aline Ritter
Paim, Aline
Bassan, Josiana Scherer
Cunha, Ana Catarina Monteiro Carvalho Mori da
dc.subject.por.fl_str_mv Cultura de tecidos
Segmentos apicais caulinares
Espécie florestal
Tissue culture
Shoot apical segments
Forestry species
topic Cultura de tecidos
Segmentos apicais caulinares
Espécie florestal
Tissue culture
Shoot apical segments
Forestry species
description The objective of this study was to evaluate the influence of several concentrations of MS medium on in vitro establishment of Peltophorum dubium shoot apical segments (80-100 mm) isolated from seedlings germinated under aseptic conditions. The salts concentrations 50, 75, 100, 125 and 150% of MS medium, were supplemented with 30 g L-1 sucrose, 100 mg L-1 of myo-inositol, 7 g L-1 agar and pH adjusted to 5.7. The experimental design was completely randomized, with 20 replications, each consisting of a vial containing one explant. The cultures were maintained for 60 days in culture room under 16-hour photoperiod, light intensity of 20 μmol m-2 s-1 and temperature of 25±2°C. There was no effect of different concentrations of MS medium for most variables. High average of in vitro survival (98%) and establishment (98%) were observed. The largest number of leaves (2.8) occurred at a dilution of 75%, decreasing from this point. At the same concentration the highest callus induction was observed, which was decreasing until not be observed under 125% of original composition; at 150% there was, again, the presence of calli. It was not observed rooting of explants during in vitro cultivation. Considering the set of variables and, additionally, that the presence of calli is not desirable, it is recommended the use of the original concentration of salts from the MS nutrient medium for the in vitro establishment of P. dubium shoot apicals segments.
publishDate 2015
dc.date.none.fl_str_mv 2015-05-12
2017-08-01T20:14:11Z
2017-08-01T20:14:11Z
2017-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv FLÔRES, A. V. et al. Estabelecimento in vitro de Peltophorum dubium ((Spreng.) Taub) em função das concentrações do meio MS. CERNE, Lavras, v. 17, n. 4, p. 549-553, out./dez. 2011.
http://repositorio.ufla.br/jspui/handle/1/14289
identifier_str_mv FLÔRES, A. V. et al. Estabelecimento in vitro de Peltophorum dubium ((Spreng.) Taub) em função das concentrações do meio MS. CERNE, Lavras, v. 17, n. 4, p. 549-553, out./dez. 2011.
url http://repositorio.ufla.br/jspui/handle/1/14289
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://www.cerne.ufla.br/site/index.php/CERNE/article/view/87/69
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Lavras (UFLA)
publisher.none.fl_str_mv Universidade Federal de Lavras (UFLA)
dc.source.none.fl_str_mv 2317-6342
0104-7760
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
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instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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