Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/28543 |
Resumo: | Oil palm is one of the most economically valuable oil seed plants, but the expansion of plantations has been limited by availability of seedlings, as the conventional propagation is through seeds, which have low germination rates. One possible solution for the large-scale production is the use of somatic embryogenesis. The aim of this study was evaluate the effects auxins 2,4-D and picloram on the induction of pro-embryogenic masses in E.guineenesis hybrid leaf explants and characterize, regarding embryogenic characteristics, with cytochemical and ultrastructural analisys. Specifically, in vitro plantlets leaves fragments were inoculated in Y3 culture medium supplemented by 2.4-D or picloram at different concentrations (0.0, 1.0, 3.0, 6.0 and 9.0 mg l-1). After 90 days the presence/ absence of cell masses were evaluated. Both growth regulators efficiently induced cellular masses regardless of the concentrations applied. As the cell masses were not homogeneously formed, they were classified according to color and shape into four types: TYPE 1 - elongated and translucent, TYPE 2 – uneven and translucent, TYPE 3 - globular and beige TYPE 4 – globular and white. Based on the anatomical and ultrastructural features, TYPE 2, 3 and 4 cell masses were considered to have the highest embryogenic potential and therefore may be most suited to large-scale vegetative propagation of oil palm |
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Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic massesEmbryogenic potentialGrowth regulatorsTissue cultureTransmission electron microscopyOil palm is one of the most economically valuable oil seed plants, but the expansion of plantations has been limited by availability of seedlings, as the conventional propagation is through seeds, which have low germination rates. One possible solution for the large-scale production is the use of somatic embryogenesis. The aim of this study was evaluate the effects auxins 2,4-D and picloram on the induction of pro-embryogenic masses in E.guineenesis hybrid leaf explants and characterize, regarding embryogenic characteristics, with cytochemical and ultrastructural analisys. Specifically, in vitro plantlets leaves fragments were inoculated in Y3 culture medium supplemented by 2.4-D or picloram at different concentrations (0.0, 1.0, 3.0, 6.0 and 9.0 mg l-1). After 90 days the presence/ absence of cell masses were evaluated. Both growth regulators efficiently induced cellular masses regardless of the concentrations applied. As the cell masses were not homogeneously formed, they were classified according to color and shape into four types: TYPE 1 - elongated and translucent, TYPE 2 – uneven and translucent, TYPE 3 - globular and beige TYPE 4 – globular and white. Based on the anatomical and ultrastructural features, TYPE 2, 3 and 4 cell masses were considered to have the highest embryogenic potential and therefore may be most suited to large-scale vegetative propagation of oil palmO dendezeiro é uma das oleoginosas mais importante economicamente, mas a expansão das plantações tem sido limitada pela disponibilidade de mudas, como a propagação convencional é através de sementes, que têm baixas taxas de germinação. Uma possível solução para produção em larga escala é por meio da embriogênese somática. O objetivo deste trabalho foi avaliar os efeitos das auxinas 2,4-D e picloram na indução de massas pró-embriogênicas em explantes foliares de Elaeis guineenesis híbrido Tenera e caracterizá-las, considerando as características embriogênicas, com analises citoquímicas e ultraestruturais. Para indução das massas pró- embriogênicas, fragmentos foliares de plântulas in vitro foram inoculados em meio de cultivo Y3 suplementado com 2,4-D ou picloram em diferentes concentrações (0,0; 1,0; 3,0; 6,0 e 9,0 mg.L–1). Após 90 dias foram avaliadas presença e ausência das massas celulares. Ambos reguladores de crescimento foram eficientes na indução destas massas, independentemente, das concentrações utilizadas. Como as massas celulares não eram homogêneas, estas foram classificadas quanto à cor e ao formato em quatro tipos: TIPO 1 – translúcido e alongado, TIPO 2 – translúcido e aquoso, TIPO 3 – bege e globular e TIPO 4 - branco e globular). De acordo com as características anatômicas e ultraestruturais os as massas celulares dos TIPOS 2, 3 e 4, foram consideradas com maior potencial embriogênico e portanto podem ser as mais promissoras para a propagação vegetativa em larga escala para o dendezeiro.Academia Brasileira de Ciências2018-02-06T15:35:56Z2018-02-06T15:35:56Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfPÁDUA, M. S. et al. Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 85, n. 4, p. 1545-1556, 2013.http://repositorio.ufla.br/jspui/handle/1/28543Anais da Academia Brasileira de Ciênciasreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessPádua, Marlúcia SouzaPaiva, Luciano VilelaAlves, EduardoLabory, Claudia Regina GontijoStein, Vanessa Cristinaeng2018-02-06T15:35:56Zoai:localhost:1/28543Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2018-02-06T15:35:56Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses |
title |
Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses |
spellingShingle |
Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses Pádua, Marlúcia Souza Embryogenic potential Growth regulators Tissue culture Transmission electron microscopy |
title_short |
Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses |
title_full |
Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses |
title_fullStr |
Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses |
title_full_unstemmed |
Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses |
title_sort |
Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses |
author |
Pádua, Marlúcia Souza |
author_facet |
Pádua, Marlúcia Souza Paiva, Luciano Vilela Alves, Eduardo Labory, Claudia Regina Gontijo Stein, Vanessa Cristina |
author_role |
author |
author2 |
Paiva, Luciano Vilela Alves, Eduardo Labory, Claudia Regina Gontijo Stein, Vanessa Cristina |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Pádua, Marlúcia Souza Paiva, Luciano Vilela Alves, Eduardo Labory, Claudia Regina Gontijo Stein, Vanessa Cristina |
dc.subject.por.fl_str_mv |
Embryogenic potential Growth regulators Tissue culture Transmission electron microscopy |
topic |
Embryogenic potential Growth regulators Tissue culture Transmission electron microscopy |
description |
Oil palm is one of the most economically valuable oil seed plants, but the expansion of plantations has been limited by availability of seedlings, as the conventional propagation is through seeds, which have low germination rates. One possible solution for the large-scale production is the use of somatic embryogenesis. The aim of this study was evaluate the effects auxins 2,4-D and picloram on the induction of pro-embryogenic masses in E.guineenesis hybrid leaf explants and characterize, regarding embryogenic characteristics, with cytochemical and ultrastructural analisys. Specifically, in vitro plantlets leaves fragments were inoculated in Y3 culture medium supplemented by 2.4-D or picloram at different concentrations (0.0, 1.0, 3.0, 6.0 and 9.0 mg l-1). After 90 days the presence/ absence of cell masses were evaluated. Both growth regulators efficiently induced cellular masses regardless of the concentrations applied. As the cell masses were not homogeneously formed, they were classified according to color and shape into four types: TYPE 1 - elongated and translucent, TYPE 2 – uneven and translucent, TYPE 3 - globular and beige TYPE 4 – globular and white. Based on the anatomical and ultrastructural features, TYPE 2, 3 and 4 cell masses were considered to have the highest embryogenic potential and therefore may be most suited to large-scale vegetative propagation of oil palm |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2018-02-06T15:35:56Z 2018-02-06T15:35:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
PÁDUA, M. S. et al. Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 85, n. 4, p. 1545-1556, 2013. http://repositorio.ufla.br/jspui/handle/1/28543 |
identifier_str_mv |
PÁDUA, M. S. et al. Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 85, n. 4, p. 1545-1556, 2013. |
url |
http://repositorio.ufla.br/jspui/handle/1/28543 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1807835090143674368 |