Avaliação do potencial antidiabético e vasodilatador dos extratos de Tournefortia paniculata Vent
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/48926 |
Resumo: | Tournefortia paniculata Vent, a synonym of Myriopus paniculatus (Cham.) Feuillet, is a plant known as marmelinho and popularly used as a diuretic and antidiabetic. Studies on its biological and phytochemical activities are scarce. Thus, this study aimed to characterize the chemical profile and the effect of Tournefortia panicuextracts on the α-glucosidase enzyme, protein glycation and vascular tone. The extracts were obtained by reflux, from the dried and pulverized, with solvents of different polarities: hexane, dichloromethane, ethyl acetate, methanol, and water. In the in vitro inhibition test of the α-glucosidase enzyme, the extracts (1mg/mL) that had the best performance were ethyl acetate (60.3 ±8%) and methanol (56.2 ±5.4%) in the other concentrations no inhibition was observed. All extracts (1mg/mL), except the aqueous extract, showed antiglycant activity: Hexane (82.5±7.5%), dichloromethane (84.3±0.88%), ethyl acetate (95.2± 1.8%) and methanol (89.7±5.6%). The evaluation of the relaxing potential after serotonin again showed that the most potent results were from the ethyl acetate (83.4 ±4.0%) and methanol (64.1 ±15.6%) extracts. The relation between the chemical profile and its biological activities was also evaluated through chromatographic profile by HPLC-DAD and principal component analysis (PCA). The PCA between the biological activities and the areas of the 10 major peaks of the chromatographic profiles indicated a positive correlation between the ethyl acetate and methanol extract and its biological effect. It is concluded that Tournefortia panicu, especially ethyl acetate and methanol extract, has potential for the prevention and treatment of diabetes, as well as for the treatment of cardiovascular diseases. |
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Avaliação do potencial antidiabético e vasodilatador dos extratos de Tournefortia paniculata VentEvaluation of the antidiabetic potential and vasodilator of the extracts of Tournefortia paniculata VentTournefortia paniculata VentMyriopus paniculatusInibição enzimáticaα-glicosidaseGlicação proteicaVasorelaxantesEnzyme inhibitionα-glycosidaseGlycationVasorelaxantManejo e Tratos CulturaisTournefortia paniculata Vent, a synonym of Myriopus paniculatus (Cham.) Feuillet, is a plant known as marmelinho and popularly used as a diuretic and antidiabetic. Studies on its biological and phytochemical activities are scarce. Thus, this study aimed to characterize the chemical profile and the effect of Tournefortia panicuextracts on the α-glucosidase enzyme, protein glycation and vascular tone. The extracts were obtained by reflux, from the dried and pulverized, with solvents of different polarities: hexane, dichloromethane, ethyl acetate, methanol, and water. In the in vitro inhibition test of the α-glucosidase enzyme, the extracts (1mg/mL) that had the best performance were ethyl acetate (60.3 ±8%) and methanol (56.2 ±5.4%) in the other concentrations no inhibition was observed. All extracts (1mg/mL), except the aqueous extract, showed antiglycant activity: Hexane (82.5±7.5%), dichloromethane (84.3±0.88%), ethyl acetate (95.2± 1.8%) and methanol (89.7±5.6%). The evaluation of the relaxing potential after serotonin again showed that the most potent results were from the ethyl acetate (83.4 ±4.0%) and methanol (64.1 ±15.6%) extracts. The relation between the chemical profile and its biological activities was also evaluated through chromatographic profile by HPLC-DAD and principal component analysis (PCA). The PCA between the biological activities and the areas of the 10 major peaks of the chromatographic profiles indicated a positive correlation between the ethyl acetate and methanol extract and its biological effect. It is concluded that Tournefortia panicu, especially ethyl acetate and methanol extract, has potential for the prevention and treatment of diabetes, as well as for the treatment of cardiovascular diseases.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Tournefortia paniculata Vent, sinonímia de Myriopus paniculatus (Cham.) Feuillet, é uma planta conhecida como marmelinho e utilizada popularmente como diurética e antidiabética. Os estudos sobre suas atividades biológicas e fitoquímica são escassos. Visto isso, objetivouse avaliar o perfil químico e o efeito dos extratos de Tournefortia paniculata sobre a enzima α- glicosidase, a glicação proteica e tônus vascular. Os extratos foram obtidos por refluxo, a partir das folhas secas e pulverizadas, com solventes de polaridades crescente, sendo eles: hexano, diclorometano, acetato de etila, metanol e água. No teste de inibição in vitro da enzima α- glicosidase os extratos (1mg/mL) que obtiveram melhor desempenho foram acetato de etila (60,3 ±8%) e metanol (56,2 ±5,4%). Em outras concentrações não foi observada inibição. Todos os extratos (1mg/mL), com exceção do aquoso, apresentaram atividade antiglicante: hexano (82,5±7,5%), diclorometano (84,3±0,88%), acetato de etila (95,2±1,8%) e metanol (89,7±5,6%). A avaliação do potencial relaxante após serotonina mostrou novamente que os resultados mais potentes foram dos extratos acetato de etila (83,4 ±4,0%) e metanol (64,1 ±15,6%). Também foi avaliada a relação entre o perfil químico e suas atividades biológicas através de análises de perfis cromatográficos por CLAE-DAD e de componentes principais (PCA). Os perfis cromatográficos dos extratos hexânico, diclometânico e acetato de etila foram mais complexos que os metanólico e aquoso. Correlação positiva entre picos majoritários dos extratos em acetato de etila e metanol e as atividades biológicas avaliadas foi observada na análise de componentes principais. Conclui-se que os extratos em acetato de etila e metanólico de Tournefortia paniculata possui potencial de prevenção e tratamento do diabetes, assim como no tratamento de doenças cardiovasculares.Universidade Federal de LavrasPrograma de Pós-Graduação em Plantas Medicinais Aromáticas e CondimentaresUFLAbrasilDepartamento de AgriculturaPereira, Aline CarvalhoBertolucci, Suzan Kelly VilelaPereira, Aline CarvalhoBertolucci, Suzan Kelly VilelaCosta, Eduardo DamascenoBittencourt, Wanderley José MantovaniCarvalho, Alexandre Alves deGonelli, Thalita2022-01-20T16:47:25Z2022-01-20T16:47:25Z2022-01-202021-10-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfGONELLI, T. Avaliação do potencial antidiabético e vasodilatador dos extratos de Tournefortia paniculata Vent. 2021. 62 p. Dissertação (Mestrado em Plantas Medicinais, Aromáticas e Condimentares) – Universidade Federal de Lavras, Lavras, 2021.http://repositorio.ufla.br/jspui/handle/1/48926porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2023-04-12T13:06:44Zoai:localhost:1/48926Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-04-12T13:06:44Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Avaliação do potencial antidiabético e vasodilatador dos extratos de Tournefortia paniculata Vent Evaluation of the antidiabetic potential and vasodilator of the extracts of Tournefortia paniculata Vent |
title |
Avaliação do potencial antidiabético e vasodilatador dos extratos de Tournefortia paniculata Vent |
spellingShingle |
Avaliação do potencial antidiabético e vasodilatador dos extratos de Tournefortia paniculata Vent Gonelli, Thalita Tournefortia paniculata Vent Myriopus paniculatus Inibição enzimática α-glicosidase Glicação proteica Vasorelaxantes Enzyme inhibition α-glycosidase Glycation Vasorelaxant Manejo e Tratos Culturais |
title_short |
Avaliação do potencial antidiabético e vasodilatador dos extratos de Tournefortia paniculata Vent |
title_full |
Avaliação do potencial antidiabético e vasodilatador dos extratos de Tournefortia paniculata Vent |
title_fullStr |
Avaliação do potencial antidiabético e vasodilatador dos extratos de Tournefortia paniculata Vent |
title_full_unstemmed |
Avaliação do potencial antidiabético e vasodilatador dos extratos de Tournefortia paniculata Vent |
title_sort |
Avaliação do potencial antidiabético e vasodilatador dos extratos de Tournefortia paniculata Vent |
author |
Gonelli, Thalita |
author_facet |
Gonelli, Thalita |
author_role |
author |
dc.contributor.none.fl_str_mv |
Pereira, Aline Carvalho Bertolucci, Suzan Kelly Vilela Pereira, Aline Carvalho Bertolucci, Suzan Kelly Vilela Costa, Eduardo Damasceno Bittencourt, Wanderley José Mantovani Carvalho, Alexandre Alves de |
dc.contributor.author.fl_str_mv |
Gonelli, Thalita |
dc.subject.por.fl_str_mv |
Tournefortia paniculata Vent Myriopus paniculatus Inibição enzimática α-glicosidase Glicação proteica Vasorelaxantes Enzyme inhibition α-glycosidase Glycation Vasorelaxant Manejo e Tratos Culturais |
topic |
Tournefortia paniculata Vent Myriopus paniculatus Inibição enzimática α-glicosidase Glicação proteica Vasorelaxantes Enzyme inhibition α-glycosidase Glycation Vasorelaxant Manejo e Tratos Culturais |
description |
Tournefortia paniculata Vent, a synonym of Myriopus paniculatus (Cham.) Feuillet, is a plant known as marmelinho and popularly used as a diuretic and antidiabetic. Studies on its biological and phytochemical activities are scarce. Thus, this study aimed to characterize the chemical profile and the effect of Tournefortia panicuextracts on the α-glucosidase enzyme, protein glycation and vascular tone. The extracts were obtained by reflux, from the dried and pulverized, with solvents of different polarities: hexane, dichloromethane, ethyl acetate, methanol, and water. In the in vitro inhibition test of the α-glucosidase enzyme, the extracts (1mg/mL) that had the best performance were ethyl acetate (60.3 ±8%) and methanol (56.2 ±5.4%) in the other concentrations no inhibition was observed. All extracts (1mg/mL), except the aqueous extract, showed antiglycant activity: Hexane (82.5±7.5%), dichloromethane (84.3±0.88%), ethyl acetate (95.2± 1.8%) and methanol (89.7±5.6%). The evaluation of the relaxing potential after serotonin again showed that the most potent results were from the ethyl acetate (83.4 ±4.0%) and methanol (64.1 ±15.6%) extracts. The relation between the chemical profile and its biological activities was also evaluated through chromatographic profile by HPLC-DAD and principal component analysis (PCA). The PCA between the biological activities and the areas of the 10 major peaks of the chromatographic profiles indicated a positive correlation between the ethyl acetate and methanol extract and its biological effect. It is concluded that Tournefortia panicu, especially ethyl acetate and methanol extract, has potential for the prevention and treatment of diabetes, as well as for the treatment of cardiovascular diseases. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-19 2022-01-20T16:47:25Z 2022-01-20T16:47:25Z 2022-01-20 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
GONELLI, T. Avaliação do potencial antidiabético e vasodilatador dos extratos de Tournefortia paniculata Vent. 2021. 62 p. Dissertação (Mestrado em Plantas Medicinais, Aromáticas e Condimentares) – Universidade Federal de Lavras, Lavras, 2021. http://repositorio.ufla.br/jspui/handle/1/48926 |
identifier_str_mv |
GONELLI, T. Avaliação do potencial antidiabético e vasodilatador dos extratos de Tournefortia paniculata Vent. 2021. 62 p. Dissertação (Mestrado em Plantas Medicinais, Aromáticas e Condimentares) – Universidade Federal de Lavras, Lavras, 2021. |
url |
http://repositorio.ufla.br/jspui/handle/1/48926 |
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por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Plantas Medicinais Aromáticas e Condimentares UFLA brasil Departamento de Agricultura |
publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Plantas Medicinais Aromáticas e Condimentares UFLA brasil Departamento de Agricultura |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
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UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
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Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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