Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/49029 |
Resumo: | Mating compatibility in ascomycetes is regulated by the mating-type locus. In this study, whole-genome sequence data were used to characterize the structural organization of the MAT loci in two heterothallic and one homothallic species of Clade 3 from Fusarium solani species complex - FSSC. Based on sequence analysis, we identified MAT1-1 idiomorph in Fusarium paranaense with three genes, MAT1-2 idiomorph in F. solani f. sp. piperis with two genes, and both MAT idiomorphs in F. striatum, homothallic species. The structural organization of the MAT1-1 locus in F. paranaense, like the MAT1-2 locus in F. striatum and F. solani f. sp. piperis was different to those of other FSSC-Clade 2 and Fusarium species. Moreover, the mating-type genes sequences in FSSC-Clade 3 were dissimilar resulting in a distinct amino acid profile. Despite these differences in the studied species, the process of sexual reproduction is not affected. Besides, we developed primers and applied in a reliable PCR protocol to MAT idiomorph identification. This paper presents the first investigation of the structural organization of the MAT locus from FSSC-Clade 3. The availability of MAT genes sequences can give support to downstream functional studies of individual mating-type transcripts. The MAT primers will be useful to assess the reproductive mode in other strains of FSSC-Clade 3, and to facilitate the application of the biological concept through sexual crosses. |
id |
UFLA_0fb1639eb3426636adc46d830e6ac0de |
---|---|
oai_identifier_str |
oai:localhost:1/49029 |
network_acronym_str |
UFLA |
network_name_str |
Repositório Institucional da UFLA |
repository_id_str |
|
spelling |
Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphsHeterothallicHomothallicMAT primersNeocosmosporaSexual reproductionWhole-genomeMating compatibility in ascomycetes is regulated by the mating-type locus. In this study, whole-genome sequence data were used to characterize the structural organization of the MAT loci in two heterothallic and one homothallic species of Clade 3 from Fusarium solani species complex - FSSC. Based on sequence analysis, we identified MAT1-1 idiomorph in Fusarium paranaense with three genes, MAT1-2 idiomorph in F. solani f. sp. piperis with two genes, and both MAT idiomorphs in F. striatum, homothallic species. The structural organization of the MAT1-1 locus in F. paranaense, like the MAT1-2 locus in F. striatum and F. solani f. sp. piperis was different to those of other FSSC-Clade 2 and Fusarium species. Moreover, the mating-type genes sequences in FSSC-Clade 3 were dissimilar resulting in a distinct amino acid profile. Despite these differences in the studied species, the process of sexual reproduction is not affected. Besides, we developed primers and applied in a reliable PCR protocol to MAT idiomorph identification. This paper presents the first investigation of the structural organization of the MAT locus from FSSC-Clade 3. The availability of MAT genes sequences can give support to downstream functional studies of individual mating-type transcripts. The MAT primers will be useful to assess the reproductive mode in other strains of FSSC-Clade 3, and to facilitate the application of the biological concept through sexual crosses.GUIMARÃES, S. S. C. et al. Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs. Current Research in Environmental & Applied Mycology, [S.l.], v. 11, n. 1, p. 159-170, 2021.Beijing Academy of Agriculture and Forestry Sciences, Institute of Plant and Environment Protection2022-01-26T00:40:36Z2022-01-26T00:40:36Z2021-05-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://repositorio.ufla.br/jspui/handle/1/49029Current Research in Environmental & Applied Mycology (CREAM)reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial-ShareAlike 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessGuimarães, S. S. C.Matteoli, F. P.Silva, F. P.Matos, K. S.Pfenning, L. H.eng2023-05-26T19:52:07Zoai:localhost:1/49029Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T19:52:07Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs |
title |
Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs |
spellingShingle |
Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs Guimarães, S. S. C. Heterothallic Homothallic MAT primers Neocosmospora Sexual reproduction Whole-genome |
title_short |
Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs |
title_full |
Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs |
title_fullStr |
Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs |
title_full_unstemmed |
Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs |
title_sort |
Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs |
author |
Guimarães, S. S. C. |
author_facet |
Guimarães, S. S. C. Matteoli, F. P. Silva, F. P. Matos, K. S. Pfenning, L. H. |
author_role |
author |
author2 |
Matteoli, F. P. Silva, F. P. Matos, K. S. Pfenning, L. H. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Guimarães, S. S. C. Matteoli, F. P. Silva, F. P. Matos, K. S. Pfenning, L. H. |
dc.subject.por.fl_str_mv |
Heterothallic Homothallic MAT primers Neocosmospora Sexual reproduction Whole-genome |
topic |
Heterothallic Homothallic MAT primers Neocosmospora Sexual reproduction Whole-genome |
description |
Mating compatibility in ascomycetes is regulated by the mating-type locus. In this study, whole-genome sequence data were used to characterize the structural organization of the MAT loci in two heterothallic and one homothallic species of Clade 3 from Fusarium solani species complex - FSSC. Based on sequence analysis, we identified MAT1-1 idiomorph in Fusarium paranaense with three genes, MAT1-2 idiomorph in F. solani f. sp. piperis with two genes, and both MAT idiomorphs in F. striatum, homothallic species. The structural organization of the MAT1-1 locus in F. paranaense, like the MAT1-2 locus in F. striatum and F. solani f. sp. piperis was different to those of other FSSC-Clade 2 and Fusarium species. Moreover, the mating-type genes sequences in FSSC-Clade 3 were dissimilar resulting in a distinct amino acid profile. Despite these differences in the studied species, the process of sexual reproduction is not affected. Besides, we developed primers and applied in a reliable PCR protocol to MAT idiomorph identification. This paper presents the first investigation of the structural organization of the MAT locus from FSSC-Clade 3. The availability of MAT genes sequences can give support to downstream functional studies of individual mating-type transcripts. The MAT primers will be useful to assess the reproductive mode in other strains of FSSC-Clade 3, and to facilitate the application of the biological concept through sexual crosses. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-05-24 2022-01-26T00:40:36Z 2022-01-26T00:40:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufla.br/jspui/handle/1/49029 |
url |
http://repositorio.ufla.br/jspui/handle/1/49029 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Beijing Academy of Agriculture and Forestry Sciences, Institute of Plant and Environment Protection |
publisher.none.fl_str_mv |
Beijing Academy of Agriculture and Forestry Sciences, Institute of Plant and Environment Protection |
dc.source.none.fl_str_mv |
Current Research in Environmental & Applied Mycology (CREAM) reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1815439282537496576 |