Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs

Detalhes bibliográficos
Autor(a) principal: Guimarães, S. S. C.
Data de Publicação: 2021
Outros Autores: Matteoli, F. P., Silva, F. P., Matos, K. S., Pfenning, L. H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/49029
Resumo: Mating compatibility in ascomycetes is regulated by the mating-type locus. In this study, whole-genome sequence data were used to characterize the structural organization of the MAT loci in two heterothallic and one homothallic species of Clade 3 from Fusarium solani species complex - FSSC. Based on sequence analysis, we identified MAT1-1 idiomorph in Fusarium paranaense with three genes, MAT1-2 idiomorph in F. solani f. sp. piperis with two genes, and both MAT idiomorphs in F. striatum, homothallic species. The structural organization of the MAT1-1 locus in F. paranaense, like the MAT1-2 locus in F. striatum and F. solani f. sp. piperis was different to those of other FSSC-Clade 2 and Fusarium species. Moreover, the mating-type genes sequences in FSSC-Clade 3 were dissimilar resulting in a distinct amino acid profile. Despite these differences in the studied species, the process of sexual reproduction is not affected. Besides, we developed primers and applied in a reliable PCR protocol to MAT idiomorph identification. This paper presents the first investigation of the structural organization of the MAT locus from FSSC-Clade 3. The availability of MAT genes sequences can give support to downstream functional studies of individual mating-type transcripts. The MAT primers will be useful to assess the reproductive mode in other strains of FSSC-Clade 3, and to facilitate the application of the biological concept through sexual crosses.
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spelling Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphsHeterothallicHomothallicMAT primersNeocosmosporaSexual reproductionWhole-genomeMating compatibility in ascomycetes is regulated by the mating-type locus. In this study, whole-genome sequence data were used to characterize the structural organization of the MAT loci in two heterothallic and one homothallic species of Clade 3 from Fusarium solani species complex - FSSC. Based on sequence analysis, we identified MAT1-1 idiomorph in Fusarium paranaense with three genes, MAT1-2 idiomorph in F. solani f. sp. piperis with two genes, and both MAT idiomorphs in F. striatum, homothallic species. The structural organization of the MAT1-1 locus in F. paranaense, like the MAT1-2 locus in F. striatum and F. solani f. sp. piperis was different to those of other FSSC-Clade 2 and Fusarium species. Moreover, the mating-type genes sequences in FSSC-Clade 3 were dissimilar resulting in a distinct amino acid profile. Despite these differences in the studied species, the process of sexual reproduction is not affected. Besides, we developed primers and applied in a reliable PCR protocol to MAT idiomorph identification. This paper presents the first investigation of the structural organization of the MAT locus from FSSC-Clade 3. The availability of MAT genes sequences can give support to downstream functional studies of individual mating-type transcripts. The MAT primers will be useful to assess the reproductive mode in other strains of FSSC-Clade 3, and to facilitate the application of the biological concept through sexual crosses.GUIMARÃES, S. S. C. et al. Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs. Current Research in Environmental & Applied Mycology, [S.l.], v. 11, n. 1, p. 159-170, 2021.Beijing Academy of Agriculture and Forestry Sciences, Institute of Plant and Environment Protection2022-01-26T00:40:36Z2022-01-26T00:40:36Z2021-05-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://repositorio.ufla.br/jspui/handle/1/49029Current Research in Environmental & Applied Mycology (CREAM)reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial-ShareAlike 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessGuimarães, S. S. C.Matteoli, F. P.Silva, F. P.Matos, K. S.Pfenning, L. H.eng2023-05-26T19:52:07Zoai:localhost:1/49029Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T19:52:07Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs
title Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs
spellingShingle Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs
Guimarães, S. S. C.
Heterothallic
Homothallic
MAT primers
Neocosmospora
Sexual reproduction
Whole-genome
title_short Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs
title_full Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs
title_fullStr Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs
title_full_unstemmed Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs
title_sort Structural organization of the mating-type locus in Clade 3 of Fusarium solani species complex and development of PCR protocol for identification of MAT idiomorphs
author Guimarães, S. S. C.
author_facet Guimarães, S. S. C.
Matteoli, F. P.
Silva, F. P.
Matos, K. S.
Pfenning, L. H.
author_role author
author2 Matteoli, F. P.
Silva, F. P.
Matos, K. S.
Pfenning, L. H.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Guimarães, S. S. C.
Matteoli, F. P.
Silva, F. P.
Matos, K. S.
Pfenning, L. H.
dc.subject.por.fl_str_mv Heterothallic
Homothallic
MAT primers
Neocosmospora
Sexual reproduction
Whole-genome
topic Heterothallic
Homothallic
MAT primers
Neocosmospora
Sexual reproduction
Whole-genome
description Mating compatibility in ascomycetes is regulated by the mating-type locus. In this study, whole-genome sequence data were used to characterize the structural organization of the MAT loci in two heterothallic and one homothallic species of Clade 3 from Fusarium solani species complex - FSSC. Based on sequence analysis, we identified MAT1-1 idiomorph in Fusarium paranaense with three genes, MAT1-2 idiomorph in F. solani f. sp. piperis with two genes, and both MAT idiomorphs in F. striatum, homothallic species. The structural organization of the MAT1-1 locus in F. paranaense, like the MAT1-2 locus in F. striatum and F. solani f. sp. piperis was different to those of other FSSC-Clade 2 and Fusarium species. Moreover, the mating-type genes sequences in FSSC-Clade 3 were dissimilar resulting in a distinct amino acid profile. Despite these differences in the studied species, the process of sexual reproduction is not affected. Besides, we developed primers and applied in a reliable PCR protocol to MAT idiomorph identification. This paper presents the first investigation of the structural organization of the MAT locus from FSSC-Clade 3. The availability of MAT genes sequences can give support to downstream functional studies of individual mating-type transcripts. The MAT primers will be useful to assess the reproductive mode in other strains of FSSC-Clade 3, and to facilitate the application of the biological concept through sexual crosses.
publishDate 2021
dc.date.none.fl_str_mv 2021-05-24
2022-01-26T00:40:36Z
2022-01-26T00:40:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufla.br/jspui/handle/1/49029
url http://repositorio.ufla.br/jspui/handle/1/49029
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution-NonCommercial-ShareAlike 4.0 International
http://creativecommons.org/licenses/by-nc-sa/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-ShareAlike 4.0 International
http://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Beijing Academy of Agriculture and Forestry Sciences, Institute of Plant and Environment Protection
publisher.none.fl_str_mv Beijing Academy of Agriculture and Forestry Sciences, Institute of Plant and Environment Protection
dc.source.none.fl_str_mv Current Research in Environmental & Applied Mycology (CREAM)
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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