Different times to perform timed artificial insemination when using a P4/E2/eCG-based protocol in buffalo
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/42793 |
Resumo: | The aim of this study was to evaluate different times for timed artificial insemination (TAI) in buffalo submitted to a P4/E2/eCG-based protocol. In this study, 204 buffaloes were distributed into one of two groups (TAI56, n=103 and TAI64, n=101). At a random stage of the oestrous cycle (Day 0 = D0), in the morning (TAI56, a.m.) or afternoon (TAI64, p.m.), buffaloes received an intravaginal progesterone device (P4; 1.0 g) plus EB (2.0 mg i.m.). On D9 a.m. (TAI56) or p.m. (TAI64), the P4 was removed and buffaloes received PGF2a (0.53 mg i.m. sodium cloprostenol) and eCG (400 IU i.m.). On D10 a.m. (TAI56) or p.m. (TAI64), 24 h after P4 removal, buffaloes were treated with EB (1.0 mg i.m.). Buffaloes from TAI56 and TAI64 were inseminated 56 and 64 h after P4 removal (D11, p.m. and D12, a.m., respectively). Ultrasound examinations were performed on D0 to ascertain ovarian follicular status, at TAI to measure the diameter of the dominant follicle (DF) and D42 for pregnancy diagnosis. The statistical analysis was performed using the GLIMMIX procedure of SAS®. There was no difference between TAI56 and TAI64 for the diameter of the DF at TAI and the pregnancy per TAI. It was concluded that TAI 56 or 64 h after P4 removal did not affect fertility in buffaloes submitted to the induction of ovulation with EB. The present research supports that is possible to perform TAI at any time throughout the day in buffalo synchronized during the non-breeding season. |
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Different times to perform timed artificial insemination when using a P4/E2/eCG-based protocol in buffaloMomentos diferentes para executar a inseminação artificial em tempo fixo utilizando o protocolo à base de P4/E2/eCG em búfalasBuffalo - ReproductionAnoestrousArtificial inseminationOestradiol benzoateFollicleInduction of ovulationBúfalas - ReproduçãoAnestroBenzoato de oestradiolFolículoIndução da ovulaçãoInseminação artificialThe aim of this study was to evaluate different times for timed artificial insemination (TAI) in buffalo submitted to a P4/E2/eCG-based protocol. In this study, 204 buffaloes were distributed into one of two groups (TAI56, n=103 and TAI64, n=101). At a random stage of the oestrous cycle (Day 0 = D0), in the morning (TAI56, a.m.) or afternoon (TAI64, p.m.), buffaloes received an intravaginal progesterone device (P4; 1.0 g) plus EB (2.0 mg i.m.). On D9 a.m. (TAI56) or p.m. (TAI64), the P4 was removed and buffaloes received PGF2a (0.53 mg i.m. sodium cloprostenol) and eCG (400 IU i.m.). On D10 a.m. (TAI56) or p.m. (TAI64), 24 h after P4 removal, buffaloes were treated with EB (1.0 mg i.m.). Buffaloes from TAI56 and TAI64 were inseminated 56 and 64 h after P4 removal (D11, p.m. and D12, a.m., respectively). Ultrasound examinations were performed on D0 to ascertain ovarian follicular status, at TAI to measure the diameter of the dominant follicle (DF) and D42 for pregnancy diagnosis. The statistical analysis was performed using the GLIMMIX procedure of SAS®. There was no difference between TAI56 and TAI64 for the diameter of the DF at TAI and the pregnancy per TAI. It was concluded that TAI 56 or 64 h after P4 removal did not affect fertility in buffaloes submitted to the induction of ovulation with EB. The present research supports that is possible to perform TAI at any time throughout the day in buffalo synchronized during the non-breeding season.O objetivo deste estudo foi avaliar diferentes momentos para a realização da IATF em búfalas submetidas a um protocolo à base de P4/E2/eCG. Neste estudo, 204 búfalas foram distribuídas em um de dois grupos (IATF56, n=103 e IATF64, n=101). Em estágio aleatório do ciclo estral (Dia 0 = D0), pela manhã (IATF56, manhã) ou pela tarde (IATF64, tarde), as búfalas receberam um dispositivo intravaginal de progesterona (P4; 1,0 g) e BE (2,0 mg i.m.). No D9 pela manhã (IATF56) ou pela tarde (IATF64), a P4 foi removida e as búfalas receberam PGF2a (0,53 mg i.m. cloprostenol sódico) e eCG (400 UI i.m.). No D10 pela manhã (IATF56) ou pela tarde (IATF64), 24 h após a remoção da P4, as búfalas foram tratadas com BE (1,0 mg i.m.). As búfalas dos grupos IATF56 e IATF64 foram inseminadas 56 e 64 h após a remoção da P4 (D11, pela tarde e D12, pela manhã, respectivamente). Avaliações ultrassonográficas foram realizadas no D0 para verificar o status folicular ovariano, na IATF para medir o diâmetro do folículo dominante (FD) e no D42 para o diagnóstico de gestação. A análise estatística foi realizada utilizando o procedimento GLIMMIX do SAS®. Não houve diferença entre os grupos IATF56 e IATF64 no diâmetro do FD na IATF e na prenhez por IATF. Conclui-se que a IATF 56 ou 64 h após a remoção da P4 não afeta a fertilidade de búfalas submetidas à indução da ovulação com BE. A presente pesquisa evidencia que é possível realizar a IATF durante todo o dia em búfalas sincronizadas durante a estação reprodutiva desfavorável.Universidade Federal de Santa Maria2020-09-01T17:40:30Z2020-09-01T17:40:30Z2020-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCARVALHO, N. A. T. da et al. Different times to perform timed artificial insemination when using a P4/E2/eCG-based protocol in buffalo. Ciência Rural, Santa Maria, v. 50, n. 4, e20190784, 2020. DOI: https://doi.org/10.1590/0103-8478cr20190784.http://repositorio.ufla.br/jspui/handle/1/42793Ciência Ruralreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessCarvalho, Nelcio Antonio Tonizza deCarvalho, Júlia Gleyci Soares deSales, José Nélio de SousaMacari, Rodrigo CaronBaruselli, Pietro Sampaioeng2020-09-01T17:40:53Zoai:localhost:1/42793Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-09-01T17:40:53Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Different times to perform timed artificial insemination when using a P4/E2/eCG-based protocol in buffalo Momentos diferentes para executar a inseminação artificial em tempo fixo utilizando o protocolo à base de P4/E2/eCG em búfalas |
title |
Different times to perform timed artificial insemination when using a P4/E2/eCG-based protocol in buffalo |
spellingShingle |
Different times to perform timed artificial insemination when using a P4/E2/eCG-based protocol in buffalo Carvalho, Nelcio Antonio Tonizza de Buffalo - Reproduction Anoestrous Artificial insemination Oestradiol benzoate Follicle Induction of ovulation Búfalas - Reprodução Anestro Benzoato de oestradiol Folículo Indução da ovulação Inseminação artificial |
title_short |
Different times to perform timed artificial insemination when using a P4/E2/eCG-based protocol in buffalo |
title_full |
Different times to perform timed artificial insemination when using a P4/E2/eCG-based protocol in buffalo |
title_fullStr |
Different times to perform timed artificial insemination when using a P4/E2/eCG-based protocol in buffalo |
title_full_unstemmed |
Different times to perform timed artificial insemination when using a P4/E2/eCG-based protocol in buffalo |
title_sort |
Different times to perform timed artificial insemination when using a P4/E2/eCG-based protocol in buffalo |
author |
Carvalho, Nelcio Antonio Tonizza de |
author_facet |
Carvalho, Nelcio Antonio Tonizza de Carvalho, Júlia Gleyci Soares de Sales, José Nélio de Sousa Macari, Rodrigo Caron Baruselli, Pietro Sampaio |
author_role |
author |
author2 |
Carvalho, Júlia Gleyci Soares de Sales, José Nélio de Sousa Macari, Rodrigo Caron Baruselli, Pietro Sampaio |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Carvalho, Nelcio Antonio Tonizza de Carvalho, Júlia Gleyci Soares de Sales, José Nélio de Sousa Macari, Rodrigo Caron Baruselli, Pietro Sampaio |
dc.subject.por.fl_str_mv |
Buffalo - Reproduction Anoestrous Artificial insemination Oestradiol benzoate Follicle Induction of ovulation Búfalas - Reprodução Anestro Benzoato de oestradiol Folículo Indução da ovulação Inseminação artificial |
topic |
Buffalo - Reproduction Anoestrous Artificial insemination Oestradiol benzoate Follicle Induction of ovulation Búfalas - Reprodução Anestro Benzoato de oestradiol Folículo Indução da ovulação Inseminação artificial |
description |
The aim of this study was to evaluate different times for timed artificial insemination (TAI) in buffalo submitted to a P4/E2/eCG-based protocol. In this study, 204 buffaloes were distributed into one of two groups (TAI56, n=103 and TAI64, n=101). At a random stage of the oestrous cycle (Day 0 = D0), in the morning (TAI56, a.m.) or afternoon (TAI64, p.m.), buffaloes received an intravaginal progesterone device (P4; 1.0 g) plus EB (2.0 mg i.m.). On D9 a.m. (TAI56) or p.m. (TAI64), the P4 was removed and buffaloes received PGF2a (0.53 mg i.m. sodium cloprostenol) and eCG (400 IU i.m.). On D10 a.m. (TAI56) or p.m. (TAI64), 24 h after P4 removal, buffaloes were treated with EB (1.0 mg i.m.). Buffaloes from TAI56 and TAI64 were inseminated 56 and 64 h after P4 removal (D11, p.m. and D12, a.m., respectively). Ultrasound examinations were performed on D0 to ascertain ovarian follicular status, at TAI to measure the diameter of the dominant follicle (DF) and D42 for pregnancy diagnosis. The statistical analysis was performed using the GLIMMIX procedure of SAS®. There was no difference between TAI56 and TAI64 for the diameter of the DF at TAI and the pregnancy per TAI. It was concluded that TAI 56 or 64 h after P4 removal did not affect fertility in buffaloes submitted to the induction of ovulation with EB. The present research supports that is possible to perform TAI at any time throughout the day in buffalo synchronized during the non-breeding season. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09-01T17:40:30Z 2020-09-01T17:40:30Z 2020-04 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
CARVALHO, N. A. T. da et al. Different times to perform timed artificial insemination when using a P4/E2/eCG-based protocol in buffalo. Ciência Rural, Santa Maria, v. 50, n. 4, e20190784, 2020. DOI: https://doi.org/10.1590/0103-8478cr20190784. http://repositorio.ufla.br/jspui/handle/1/42793 |
identifier_str_mv |
CARVALHO, N. A. T. da et al. Different times to perform timed artificial insemination when using a P4/E2/eCG-based protocol in buffalo. Ciência Rural, Santa Maria, v. 50, n. 4, e20190784, 2020. DOI: https://doi.org/10.1590/0103-8478cr20190784. |
url |
http://repositorio.ufla.br/jspui/handle/1/42793 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835155099811840 |