Microenxertia de Selenicereus megalanthus em diferentes porta-enxertos de pitaia

Detalhes bibliográficos
Autor(a) principal: Moreira, Renata Amato
Data de Publicação: 2018
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/29341
Resumo: Pitaia has aroused the planting interest of Brazilian producers and the researchers' interest in improving their production system, due to their rusticity, precocity of production and high economic returns. The objective of this research was to evaluate the viability of yellow pitaia (Selenicereus megalanthus) micrografting on differents rootstocks. Five treatments were used, consisting of white pitaia rootstocks (Hylocereus undatus), red pitaias (Hylocereus polyrhizus) Cebra and Orejana varieties and yellow pitaia (Selenicereus megalanthus), four replicates of five plants, in a total of 100 plants. For evaluation, after 30 days of cultivation, ten plants of each treatment were evaluated for the length and diameter of scions and rootstocks, root length, percentage of "glue" of the micrografts, as well as the fresh mass of the plants. Subsequently, the micrografted plants were taken to the greenhouse to be acclimatized and at 90 days, phytotechnical analyzes were performed, measuring the length and diameter of the scion and rootstock. Flow cytometric analyzes were also performed, which were conducted before and after the micrografting procedure. Before micrografting, 4 plants (cladodes) of each pitaias established in vitro were sampled and after micrografting, 3 plants per treatment of both the scion and the rootstock were evaluated to verify possible changes in their DNA index. After 30 days of in vitro culture of the micrografted plants, longitudinal cuts were also made in the region of insertion of the scion with the rootstock, aiming to verify the presence of the connections of vessels and tissues between them, through histological cuts. Comparing the DNA indices, there was endoreduplication in all treatments and when the yellow pitaia was grafted onto the red Orejana variety rootstock, the genetic material index of the scion was not affected. Considering phytotechnical, genetic and anatomical characteristics, it is feasible the micrografting of yellow pitaia.
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spelling Microenxertia de Selenicereus megalanthus em diferentes porta-enxertos de pitaiaMicrografting of Selenicereus megalanthus in differents rootstocks of pitaiaEnxertoPitaiaMicropropagação de plantasPropagação vegetativaScionPitayaPlant micropropagationVegetative propagationFitotecniaPitaia has aroused the planting interest of Brazilian producers and the researchers' interest in improving their production system, due to their rusticity, precocity of production and high economic returns. The objective of this research was to evaluate the viability of yellow pitaia (Selenicereus megalanthus) micrografting on differents rootstocks. Five treatments were used, consisting of white pitaia rootstocks (Hylocereus undatus), red pitaias (Hylocereus polyrhizus) Cebra and Orejana varieties and yellow pitaia (Selenicereus megalanthus), four replicates of five plants, in a total of 100 plants. For evaluation, after 30 days of cultivation, ten plants of each treatment were evaluated for the length and diameter of scions and rootstocks, root length, percentage of "glue" of the micrografts, as well as the fresh mass of the plants. Subsequently, the micrografted plants were taken to the greenhouse to be acclimatized and at 90 days, phytotechnical analyzes were performed, measuring the length and diameter of the scion and rootstock. Flow cytometric analyzes were also performed, which were conducted before and after the micrografting procedure. Before micrografting, 4 plants (cladodes) of each pitaias established in vitro were sampled and after micrografting, 3 plants per treatment of both the scion and the rootstock were evaluated to verify possible changes in their DNA index. After 30 days of in vitro culture of the micrografted plants, longitudinal cuts were also made in the region of insertion of the scion with the rootstock, aiming to verify the presence of the connections of vessels and tissues between them, through histological cuts. Comparing the DNA indices, there was endoreduplication in all treatments and when the yellow pitaia was grafted onto the red Orejana variety rootstock, the genetic material index of the scion was not affected. Considering phytotechnical, genetic and anatomical characteristics, it is feasible the micrografting of yellow pitaia.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)A pitaia vem despertando o interesse de plantio dos produtores brasileiros e o interesse dos pesquisadores na melhoria do seu sistema de produção devido à sua rusticidade, precocidade de produção e alto retorno econômico. Assim, objetivou-se avaliar a viabilidade da microenxertia de pitaia amarela (Selenicereus megalanthus) sobre diferentes porta-enxertos. Utilizou-se cinco tratamentos, constituídos pelos porta-enxertos da pitaia branca (Hylocereus undatus), saborosa (Selenicereus setaceus), pitaias vermelhas (Hylocereus polyrhizus), variedades Cebra, Orejana e pitaia amarela(Selenicereus megalanthus), contendo quatro repetições de cinco plantas, num total de 100 plantas. Para avaliação considerou-se, após 30 dias de cultivo, dez plantas de cada tratamento avaliadas quanto ao comprimento e diâmetro dos enxertos e porta-enxertos, comprimento de raízes, porcentagem de “pegamento” dos microenxertos, bem como a massa fresca das plantas. Posteriormente, as plantas microenxertadas foram levadas para a casa de vegetação para serem aclimatizadas e aos 90 dias, foram feitas análises fitotécnicas, medindo-se o comprimento e diâmetro do enxerto e porta-enxerto. Procedeu-se também análises de citometria de fluxo, as quais foram conduzidas antes e depois do procedimento de microenxertia. Antes da microenxertia foram amostradas 4 plantas (cladódios) de cada pitaia estabelecida in vitro e após a microenxertia, avaliou-se 3 plantas por tratamento, tanto do enxerto quanto do porta-enxerto, para verificação de possíveis mudanças no índice de DNA das mesmas. Após 30 dias de cultivo in vitro das plantas microenxertadas, também foram realizados cortes longitudinais na região de inserção do enxerto com o porta-enxerto visando verificar a presença das conexões de vasos e tecidos entre eles, por meio de cortes histológicos. Comparando os índices de DNA, houve endoreduplicação em todos os tratamentos e, quando a pitaia amarela foi enxertada sobre o porta-enxerto da vermelha variedade Orejana, o índice de material genético do enxerto não foi afetado. Considerando características fitotecnicas, genéticas e anatômicas, é viável a microenxertia de pitaia amarela.Universidade Federal de LavrasAgronomia/FitotecniaUFLAbrasilDepartamento de AgriculturaPio, Leila Aparecida SallesSantos, Dalilhia Nazaré dosSantos, Dalilhia Nazaré dosCosta, Ana ClaudiaGuimarães, Paulo Henrique SalesMoreira, Renata Amato2018-06-06T14:01:31Z2018-06-06T14:01:31Z2018-06-042018-04-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfMOREIRA, R. A. Microenxertia de Selenicereus megalanthus em diferentes porta-enxertos de pitaia. 2018. 45 p. Dissertação (Mestrado em Agronomia/Fitotecnia)–Universidade Federal de Lavras, Lavras, 2018.http://repositorio.ufla.br/jspui/handle/1/29341porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2018-06-06T14:01:31Zoai:localhost:1/29341Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2018-06-06T14:01:31Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Microenxertia de Selenicereus megalanthus em diferentes porta-enxertos de pitaia
Micrografting of Selenicereus megalanthus in differents rootstocks of pitaia
title Microenxertia de Selenicereus megalanthus em diferentes porta-enxertos de pitaia
spellingShingle Microenxertia de Selenicereus megalanthus em diferentes porta-enxertos de pitaia
Moreira, Renata Amato
Enxerto
Pitaia
Micropropagação de plantas
Propagação vegetativa
Scion
Pitaya
Plant micropropagation
Vegetative propagation
Fitotecnia
title_short Microenxertia de Selenicereus megalanthus em diferentes porta-enxertos de pitaia
title_full Microenxertia de Selenicereus megalanthus em diferentes porta-enxertos de pitaia
title_fullStr Microenxertia de Selenicereus megalanthus em diferentes porta-enxertos de pitaia
title_full_unstemmed Microenxertia de Selenicereus megalanthus em diferentes porta-enxertos de pitaia
title_sort Microenxertia de Selenicereus megalanthus em diferentes porta-enxertos de pitaia
author Moreira, Renata Amato
author_facet Moreira, Renata Amato
author_role author
dc.contributor.none.fl_str_mv Pio, Leila Aparecida Salles
Santos, Dalilhia Nazaré dos
Santos, Dalilhia Nazaré dos
Costa, Ana Claudia
Guimarães, Paulo Henrique Sales
dc.contributor.author.fl_str_mv Moreira, Renata Amato
dc.subject.por.fl_str_mv Enxerto
Pitaia
Micropropagação de plantas
Propagação vegetativa
Scion
Pitaya
Plant micropropagation
Vegetative propagation
Fitotecnia
topic Enxerto
Pitaia
Micropropagação de plantas
Propagação vegetativa
Scion
Pitaya
Plant micropropagation
Vegetative propagation
Fitotecnia
description Pitaia has aroused the planting interest of Brazilian producers and the researchers' interest in improving their production system, due to their rusticity, precocity of production and high economic returns. The objective of this research was to evaluate the viability of yellow pitaia (Selenicereus megalanthus) micrografting on differents rootstocks. Five treatments were used, consisting of white pitaia rootstocks (Hylocereus undatus), red pitaias (Hylocereus polyrhizus) Cebra and Orejana varieties and yellow pitaia (Selenicereus megalanthus), four replicates of five plants, in a total of 100 plants. For evaluation, after 30 days of cultivation, ten plants of each treatment were evaluated for the length and diameter of scions and rootstocks, root length, percentage of "glue" of the micrografts, as well as the fresh mass of the plants. Subsequently, the micrografted plants were taken to the greenhouse to be acclimatized and at 90 days, phytotechnical analyzes were performed, measuring the length and diameter of the scion and rootstock. Flow cytometric analyzes were also performed, which were conducted before and after the micrografting procedure. Before micrografting, 4 plants (cladodes) of each pitaias established in vitro were sampled and after micrografting, 3 plants per treatment of both the scion and the rootstock were evaluated to verify possible changes in their DNA index. After 30 days of in vitro culture of the micrografted plants, longitudinal cuts were also made in the region of insertion of the scion with the rootstock, aiming to verify the presence of the connections of vessels and tissues between them, through histological cuts. Comparing the DNA indices, there was endoreduplication in all treatments and when the yellow pitaia was grafted onto the red Orejana variety rootstock, the genetic material index of the scion was not affected. Considering phytotechnical, genetic and anatomical characteristics, it is feasible the micrografting of yellow pitaia.
publishDate 2018
dc.date.none.fl_str_mv 2018-06-06T14:01:31Z
2018-06-06T14:01:31Z
2018-06-04
2018-04-27
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv MOREIRA, R. A. Microenxertia de Selenicereus megalanthus em diferentes porta-enxertos de pitaia. 2018. 45 p. Dissertação (Mestrado em Agronomia/Fitotecnia)–Universidade Federal de Lavras, Lavras, 2018.
http://repositorio.ufla.br/jspui/handle/1/29341
identifier_str_mv MOREIRA, R. A. Microenxertia de Selenicereus megalanthus em diferentes porta-enxertos de pitaia. 2018. 45 p. Dissertação (Mestrado em Agronomia/Fitotecnia)–Universidade Federal de Lavras, Lavras, 2018.
url http://repositorio.ufla.br/jspui/handle/1/29341
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Lavras
Agronomia/Fitotecnia
UFLA
brasil
Departamento de Agricultura
publisher.none.fl_str_mv Universidade Federal de Lavras
Agronomia/Fitotecnia
UFLA
brasil
Departamento de Agricultura
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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