Molecular characterization of elephantgrass accessions through RAPD markers

Detalhes bibliográficos
Autor(a) principal: Passos, Leônidas Paixão
Data de Publicação: 2005
Outros Autores: Machado, Marco Antonio, Vidigal, Maria Coletta, Campos, Ana Lúcia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542005000300009
http://repositorio.ufla.br/jspui/handle/1/6069
Resumo: Elephantgrass pastures are limited by yield variations and reductions in forage quality and availability, thus making the search for genotypes with reduced seasonality a major concern. In order to verify the extent of genetic variability among contrasting cultivars, ten elephantgrass accessions were analyzed through DNA amplification by RAPD technique. A total of 160 DNA bands were generated with the use of 44 random primers and 23% of these bands were monomorphic for all accessions. Gel-obtained binary data (1 for presence and 0 for absence) were used for generating a genetic distance matrix, which was utilized in a UPGMA grouping analysis. Elephantgrass cultivars Cameroon and Vruckwona were the accessions mostly divergent from the others, with an average genetic distance of 0.34. The accessions with the lowest average genetic distances from the others were Pioneiro and CNPGL 27-5, both with a distance of 0.25. Overall, genetic distances ranged from 0.06 to 0.43, indicating little genetic variability for the set of accessions, despite the contrasting morphology of the studied genotypes.
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spelling Molecular characterization of elephantgrass accessions through RAPD markersCaracterização molecular de acessos de capim-elefante utilizando marcadores RAPDMolecular markersElephantgrassRAPDDNAMarcadores molecularesCapim-elefanteElephantgrass pastures are limited by yield variations and reductions in forage quality and availability, thus making the search for genotypes with reduced seasonality a major concern. In order to verify the extent of genetic variability among contrasting cultivars, ten elephantgrass accessions were analyzed through DNA amplification by RAPD technique. A total of 160 DNA bands were generated with the use of 44 random primers and 23% of these bands were monomorphic for all accessions. Gel-obtained binary data (1 for presence and 0 for absence) were used for generating a genetic distance matrix, which was utilized in a UPGMA grouping analysis. Elephantgrass cultivars Cameroon and Vruckwona were the accessions mostly divergent from the others, with an average genetic distance of 0.34. The accessions with the lowest average genetic distances from the others were Pioneiro and CNPGL 27-5, both with a distance of 0.25. Overall, genetic distances ranged from 0.06 to 0.43, indicating little genetic variability for the set of accessions, despite the contrasting morphology of the studied genotypes.O cultivo do capim-elefante é limitado por variações na produção de biomassa e por reduções na qualidade e disponibilidade de forragem, tornando prioritária a busca de genótipos com menor sazonalidade de produção. Para verificar a extensão da variabilidade genética entre cultivares contrastantes, 10 acessos foram analisados via amplificação do DNA pela técnica RAPD. Foram utilizados 44 primers aleatórios, que produziram um total de 160 bandas de DNA, sendo que 23% das bandas analisadas foram monomórficas para todos os acessos. Os dados binários, originados dos géis (1-presença, 0-ausência), foram utilizados para gerar uma matriz de distâncias genéticas, que foi utilizada para uma análise de agrupamento segundo o método da média das distâncias (UPGMA). Os acessos mais divergentes dos demais foram Cameroon e Vruckwona, com distâncias genéticas médias de 0,34. Os acessos com menores distâncias genéticas médias dos demais foram Pioneiro e CNPGL 27-5, ambos com uma distância genética média de 0,25. As distâncias genéticas, considerando todas as análises, variaram de 0,06 a 0,43, indicando uma variabilidade genética pouco acentuada, embora os acessos estudados sejam bastante contrastantes em relação à morfologia e fisiologia.Editora da Universidade Federal de Lavras2005-06-012015-04-30T13:33:29Z2015-04-30T13:33:29Z2015-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542005000300009PASSOS, L. P. et al. Molecular characterization of elephantgrass accessions through RAPD markers. Ciência e Agrotecnologia, Lavras, v. 29, n. 3, p. 568-574, maio/jun. 2005.http://repositorio.ufla.br/jspui/handle/1/6069Ciência e Agrotecnologia v.29 n.3 2005reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAPassos, Leônidas PaixãoMachado, Marco AntonioVidigal, Maria ColettaCampos, Ana Lúciaenginfo:eu-repo/semantics/openAccess2016-07-21T19:09:25Zoai:localhost:1/6069Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2016-07-21T19:09:25Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Molecular characterization of elephantgrass accessions through RAPD markers
Caracterização molecular de acessos de capim-elefante utilizando marcadores RAPD
title Molecular characterization of elephantgrass accessions through RAPD markers
spellingShingle Molecular characterization of elephantgrass accessions through RAPD markers
Passos, Leônidas Paixão
Molecular markers
Elephantgrass
RAPD
DNA
Marcadores moleculares
Capim-elefante
title_short Molecular characterization of elephantgrass accessions through RAPD markers
title_full Molecular characterization of elephantgrass accessions through RAPD markers
title_fullStr Molecular characterization of elephantgrass accessions through RAPD markers
title_full_unstemmed Molecular characterization of elephantgrass accessions through RAPD markers
title_sort Molecular characterization of elephantgrass accessions through RAPD markers
author Passos, Leônidas Paixão
author_facet Passos, Leônidas Paixão
Machado, Marco Antonio
Vidigal, Maria Coletta
Campos, Ana Lúcia
author_role author
author2 Machado, Marco Antonio
Vidigal, Maria Coletta
Campos, Ana Lúcia
author2_role author
author
author
dc.contributor.author.fl_str_mv Passos, Leônidas Paixão
Machado, Marco Antonio
Vidigal, Maria Coletta
Campos, Ana Lúcia
dc.subject.por.fl_str_mv Molecular markers
Elephantgrass
RAPD
DNA
Marcadores moleculares
Capim-elefante
topic Molecular markers
Elephantgrass
RAPD
DNA
Marcadores moleculares
Capim-elefante
description Elephantgrass pastures are limited by yield variations and reductions in forage quality and availability, thus making the search for genotypes with reduced seasonality a major concern. In order to verify the extent of genetic variability among contrasting cultivars, ten elephantgrass accessions were analyzed through DNA amplification by RAPD technique. A total of 160 DNA bands were generated with the use of 44 random primers and 23% of these bands were monomorphic for all accessions. Gel-obtained binary data (1 for presence and 0 for absence) were used for generating a genetic distance matrix, which was utilized in a UPGMA grouping analysis. Elephantgrass cultivars Cameroon and Vruckwona were the accessions mostly divergent from the others, with an average genetic distance of 0.34. The accessions with the lowest average genetic distances from the others were Pioneiro and CNPGL 27-5, both with a distance of 0.25. Overall, genetic distances ranged from 0.06 to 0.43, indicating little genetic variability for the set of accessions, despite the contrasting morphology of the studied genotypes.
publishDate 2005
dc.date.none.fl_str_mv 2005-06-01
2015-04-30T13:33:29Z
2015-04-30T13:33:29Z
2015-04-30
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542005000300009
PASSOS, L. P. et al. Molecular characterization of elephantgrass accessions through RAPD markers. Ciência e Agrotecnologia, Lavras, v. 29, n. 3, p. 568-574, maio/jun. 2005.
http://repositorio.ufla.br/jspui/handle/1/6069
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542005000300009
http://repositorio.ufla.br/jspui/handle/1/6069
identifier_str_mv PASSOS, L. P. et al. Molecular characterization of elephantgrass accessions through RAPD markers. Ciência e Agrotecnologia, Lavras, v. 29, n. 3, p. 568-574, maio/jun. 2005.
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Editora da Universidade Federal de Lavras
publisher.none.fl_str_mv Editora da Universidade Federal de Lavras
dc.source.none.fl_str_mv Ciência e Agrotecnologia v.29 n.3 2005
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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