Influence of soil temperature and moisture on the infectivity of entomopathogenic nematodes (Rhabditida: Heterorhabditidae, Steinernematidae) against larvae of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)

• Autor(a) principal: Rohde, Cristhiane
• Data de Publicação: 2010
• Outros Autores: Moino Junior, Alcides, Silva, Marco Aurélio Tramontim da, Carvalho, Fabiano Duarte, Ferreira, Cleidson Soares
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFLA
• Texto Completo: http://repositorio.ufla.br/jspui/handle/1/11683
• topic: Nematóides – Patogênese; Frutas – Doenças e pragas - Controle biológico; Pragas – Controle biológico - Efeito da umidade do solo; Pragas – Controle biológico - Efeito da temperatura; Nematoda – Pathogenesis; Fruit – Disseases and pests - Biological control; Pests – Biological control - Effect of soil moisture; Pests – Biological control - Effect of temperature; Heterorhabditis sp.; Steinernema carpocapsae; Ceratitis capitata

The Mediterranean fruit fly, Ceratitis capitata (Wiedemann), is considered one of the main pests that affect fruit production in the world. This insect spends part of its life cycle in the soil, making it a target for entomopathogenic nematodes. This work aimed at evaluating the influence of soil temperature and moisture on the infectivity of Heterorhabditis sp. RSC01 and Steinernema carpocapsae ALL to third-instars of C. capitata, and to compare the efficiency of these isolates at five different soil temperatures (19, 22, 25, 28, and 31°C) and three levels of relative soil moisture (100, 75, and 50% of field capacity). Ten C. capitata larvae were transferred to plastic jars (12 cm × 6 cm) containing 100 g soil, followed by the application of an aqueous suspension containing 125 infective juveniles (IJ)/cm2. In the control treatment, 3 ml of distilled water was applied. Mortality evaluations were made five days later and were confirmed by observations of the characteristic symptoms and cadaver dissection. The infectivity was directly proportional to temperature increase, with maximum percent mortality of 86.7% and 80.0% for S. carpocapsae and Heterorhabditis sp., respectively, at 31°C. At 25°C, the highest mortality for both species was obtained at 75% of field capacity (96.7% and 26.7% for S. carpocapsae and Heterorhabditis sp., respectively).