Embryogenic induction of torch ginger (Etlingera elatior) callus
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/38379 |
Resumo: | Torch ginger Etlingera elatior (Jack) R.M. Smith is a tropical herb-like, rhizome-like and perennial plant that possesses inflorescences that are both pretty and showy at different colour shades, and have a great visual appeal. Producing seedlings at a high enough level when done conventionally by the division of tussocks, has high costs and phytosanitary problems. The objective of this study was to evaluate the induction of somatic embryogenesis and its potential for subsequent plant regeneration in torch ginger. Rhizomes, leaves and roots from the seedlings grown in vitro were used as explant segments. The different explants were cultured on MS medium supplemented with 30 g L-1 sucrose, and it was solidified with 5.5 g L-1 of agar. The effect of 2.4-D and picloram concentrations of 0.0, 1.0, 2.0 and 4.0 mg L-1 was studied. Astructural analysis was performed by scanning electron microscopy (SEM). Cytochemical analysis were performed to detect nucleeous with carmine acetic, starch with lugol and lipids with Sudan III, and a flow cytometry analysis to verify the stability of ploidy in the callus. Results indicate that the culture medium with 2.4-D yielded the induction of embryogenic callus with the characteristics of the rhizome segments of the explant. Regarding the ultrastructural analysis of embryogenic callus characteristics, the cells showed a similar isodiametric form as somatic embryos in the globular stage and cytochemical analysis. The presence of callus cells in pro-embryogenic explants containing each of the regulators was confirmed. In the cytometry analysis, the average DNA content of callus ranged from 5.43 to 10.15 pg, and the coefficients of variation varied between 0.3 to 2.26. Callus induced with 2.4-D showed 86 % stability, and with picloram was 64 %. The callus formation was more evident after application of 2.4 D. This is the first study of embryogenic potential in cells of the torch ginger, and showed that 2,4-D induced callus with higher starch content and genetically stable. |
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Embryogenic induction of torch ginger (Etlingera elatior) callusInducción embriogénica en callo de jengibre antorcha (Etlingera elatior)ZingiberaceaeEtlingera elatiorViabilityViabilidadTorch ginger Etlingera elatior (Jack) R.M. Smith is a tropical herb-like, rhizome-like and perennial plant that possesses inflorescences that are both pretty and showy at different colour shades, and have a great visual appeal. Producing seedlings at a high enough level when done conventionally by the division of tussocks, has high costs and phytosanitary problems. The objective of this study was to evaluate the induction of somatic embryogenesis and its potential for subsequent plant regeneration in torch ginger. Rhizomes, leaves and roots from the seedlings grown in vitro were used as explant segments. The different explants were cultured on MS medium supplemented with 30 g L-1 sucrose, and it was solidified with 5.5 g L-1 of agar. The effect of 2.4-D and picloram concentrations of 0.0, 1.0, 2.0 and 4.0 mg L-1 was studied. Astructural analysis was performed by scanning electron microscopy (SEM). Cytochemical analysis were performed to detect nucleeous with carmine acetic, starch with lugol and lipids with Sudan III, and a flow cytometry analysis to verify the stability of ploidy in the callus. Results indicate that the culture medium with 2.4-D yielded the induction of embryogenic callus with the characteristics of the rhizome segments of the explant. Regarding the ultrastructural analysis of embryogenic callus characteristics, the cells showed a similar isodiametric form as somatic embryos in the globular stage and cytochemical analysis. The presence of callus cells in pro-embryogenic explants containing each of the regulators was confirmed. In the cytometry analysis, the average DNA content of callus ranged from 5.43 to 10.15 pg, and the coefficients of variation varied between 0.3 to 2.26. Callus induced with 2.4-D showed 86 % stability, and with picloram was 64 %. The callus formation was more evident after application of 2.4 D. This is the first study of embryogenic potential in cells of the torch ginger, and showed that 2,4-D induced callus with higher starch content and genetically stable.El jengibre antorcha Etlingera elatior (Jack) R. M. Smith es una planta tropical perenne, similar al rizoma, que tiene inflorescencias que son bellas y llamativas, de diferentes colores y matices y de un gran atractivo visual. La producción de plántulas en un nivel lo suficientemente alto cuando se hace convencionalmente utilizando la división de matas tiene altos costos y problemas fitosanitarios. El objetivo de este estudio fue evaluar la inducción de la embriogénesis somática y su uso potencial para la reproducción posterior de plantas de jengibre antorcha. Los rizomas, hojas y raíces de las plántulas cultivadas in vitro se utilizaron como segmentos de explantes. Los diferentes explantes se cultivaron en un medio MS al que se le agregaron 30 g L-1 de sacarosa y fue solidificado con 5.5 g de agar L-1. Se estudió el efecto del 2.4-D y picloram en concentraciones de 0.0, 1.0, 2.0 y 4.0 mg L-1. El análisis estructural se llevó a cabo por microscopía electrónica de barrido (SEM). Se realizaron análisis citoquímicos para detectar núcleos con carmín acético, almidón con lugol y lípidos con Sudán III; y un análisis de citometría de flujo para verificar la estabilidad de la ploidía en el callo. Los resultados indicaron que el medio de cultivo con 2.4-D produjo la inducción de callo embriogénico con las características de los segmentos de rizomas del explante. En el análisis ultraestructural de las características del callo embriogénico, las células mostraron una forma isodiamétrica similar a embriones somáticos en etapa globular y análisis citoquímico. Se confirmó la presencia de células de callo en explantes pro- embriogénicos conteniendo cada uno de los reguladores. En el análisis de citometría, el contenido medio de ADN del callo varió de 5.43 a 10.15 pg, y los coeficientes de variación oscilaron entre 0.3 y 2.26. El 2.4-D indujo callos con estabilidad de 86 %, y con picloram fue 64 %. La formación de callo fue más evidente después de aplicar 2.4-D. Éste es el primer estudio del potencial embriogénico de células del jengibre antorcha, y mostró que el 2.4-D indujo la formación de callo con mayor contenido de almidón y genéticamente estable.Colegio de Postgraduados2019-12-30T18:18:42Z2019-12-30T18:18:42Z2014-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfGOMES-DÍAS, G. de M. et al. Embryogenic induction of torch ginger (Etlingera elatior) callus. Agrociencia, Texcoco, v. 48, n. 2, p. 173-184, Feb./Mar. 2014.http://repositorio.ufla.br/jspui/handle/1/38379Agrocienciareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessGomes-Dias, Gabrielen de M.Almendagna-Rodrigues, FilipeRodrigues-Soares, DóriaPasqual, MoacirCarvalho, A. Cristina Portugal-Pinto deeng2023-05-26T18:42:15Zoai:localhost:1/38379Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:42:15Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Embryogenic induction of torch ginger (Etlingera elatior) callus Inducción embriogénica en callo de jengibre antorcha (Etlingera elatior) |
title |
Embryogenic induction of torch ginger (Etlingera elatior) callus |
spellingShingle |
Embryogenic induction of torch ginger (Etlingera elatior) callus Gomes-Dias, Gabrielen de M. Zingiberaceae Etlingera elatior Viability Viabilidad |
title_short |
Embryogenic induction of torch ginger (Etlingera elatior) callus |
title_full |
Embryogenic induction of torch ginger (Etlingera elatior) callus |
title_fullStr |
Embryogenic induction of torch ginger (Etlingera elatior) callus |
title_full_unstemmed |
Embryogenic induction of torch ginger (Etlingera elatior) callus |
title_sort |
Embryogenic induction of torch ginger (Etlingera elatior) callus |
author |
Gomes-Dias, Gabrielen de M. |
author_facet |
Gomes-Dias, Gabrielen de M. Almendagna-Rodrigues, Filipe Rodrigues-Soares, Dória Pasqual, Moacir Carvalho, A. Cristina Portugal-Pinto de |
author_role |
author |
author2 |
Almendagna-Rodrigues, Filipe Rodrigues-Soares, Dória Pasqual, Moacir Carvalho, A. Cristina Portugal-Pinto de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Gomes-Dias, Gabrielen de M. Almendagna-Rodrigues, Filipe Rodrigues-Soares, Dória Pasqual, Moacir Carvalho, A. Cristina Portugal-Pinto de |
dc.subject.por.fl_str_mv |
Zingiberaceae Etlingera elatior Viability Viabilidad |
topic |
Zingiberaceae Etlingera elatior Viability Viabilidad |
description |
Torch ginger Etlingera elatior (Jack) R.M. Smith is a tropical herb-like, rhizome-like and perennial plant that possesses inflorescences that are both pretty and showy at different colour shades, and have a great visual appeal. Producing seedlings at a high enough level when done conventionally by the division of tussocks, has high costs and phytosanitary problems. The objective of this study was to evaluate the induction of somatic embryogenesis and its potential for subsequent plant regeneration in torch ginger. Rhizomes, leaves and roots from the seedlings grown in vitro were used as explant segments. The different explants were cultured on MS medium supplemented with 30 g L-1 sucrose, and it was solidified with 5.5 g L-1 of agar. The effect of 2.4-D and picloram concentrations of 0.0, 1.0, 2.0 and 4.0 mg L-1 was studied. Astructural analysis was performed by scanning electron microscopy (SEM). Cytochemical analysis were performed to detect nucleeous with carmine acetic, starch with lugol and lipids with Sudan III, and a flow cytometry analysis to verify the stability of ploidy in the callus. Results indicate that the culture medium with 2.4-D yielded the induction of embryogenic callus with the characteristics of the rhizome segments of the explant. Regarding the ultrastructural analysis of embryogenic callus characteristics, the cells showed a similar isodiametric form as somatic embryos in the globular stage and cytochemical analysis. The presence of callus cells in pro-embryogenic explants containing each of the regulators was confirmed. In the cytometry analysis, the average DNA content of callus ranged from 5.43 to 10.15 pg, and the coefficients of variation varied between 0.3 to 2.26. Callus induced with 2.4-D showed 86 % stability, and with picloram was 64 %. The callus formation was more evident after application of 2.4 D. This is the first study of embryogenic potential in cells of the torch ginger, and showed that 2,4-D induced callus with higher starch content and genetically stable. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-03 2019-12-30T18:18:42Z 2019-12-30T18:18:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
GOMES-DÍAS, G. de M. et al. Embryogenic induction of torch ginger (Etlingera elatior) callus. Agrociencia, Texcoco, v. 48, n. 2, p. 173-184, Feb./Mar. 2014. http://repositorio.ufla.br/jspui/handle/1/38379 |
identifier_str_mv |
GOMES-DÍAS, G. de M. et al. Embryogenic induction of torch ginger (Etlingera elatior) callus. Agrociencia, Texcoco, v. 48, n. 2, p. 173-184, Feb./Mar. 2014. |
url |
http://repositorio.ufla.br/jspui/handle/1/38379 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Colegio de Postgraduados |
publisher.none.fl_str_mv |
Colegio de Postgraduados |
dc.source.none.fl_str_mv |
Agrociencia reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1815439064997822464 |