Embryogenic induction of torch ginger (Etlingera elatior) callus

Detalhes bibliográficos
Autor(a) principal: Gomes-Dias, Gabrielen de M.
Data de Publicação: 2014
Outros Autores: Almendagna-Rodrigues, Filipe, Rodrigues-Soares, Dória, Pasqual, Moacir, Carvalho, A. Cristina Portugal-Pinto de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/38379
Resumo: Torch ginger Etlingera elatior (Jack) R.M. Smith is a tropical herb-like, rhizome-like and perennial plant that possesses inflorescences that are both pretty and showy at different colour shades, and have a great visual appeal. Producing seedlings at a high enough level when done conventionally by the division of tussocks, has high costs and phytosanitary problems. The objective of this study was to evaluate the induction of somatic embryogenesis and its potential for subsequent plant regeneration in torch ginger. Rhizomes, leaves and roots from the seedlings grown in vitro were used as explant segments. The different explants were cultured on MS medium supplemented with 30 g L-1 sucrose, and it was solidified with 5.5 g L-1 of agar. The effect of 2.4-D and picloram concentrations of 0.0, 1.0, 2.0 and 4.0 mg L-1 was studied. Astructural analysis was performed by scanning electron microscopy (SEM). Cytochemical analysis were performed to detect nucleeous with carmine acetic, starch with lugol and lipids with Sudan III, and a flow cytometry analysis to verify the stability of ploidy in the callus. Results indicate that the culture medium with 2.4-D yielded the induction of embryogenic callus with the characteristics of the rhizome segments of the explant. Regarding the ultrastructural analysis of embryogenic callus characteristics, the cells showed a similar isodiametric form as somatic embryos in the globular stage and cytochemical analysis. The presence of callus cells in pro-embryogenic explants containing each of the regulators was confirmed. In the cytometry analysis, the average DNA content of callus ranged from 5.43 to 10.15 pg, and the coefficients of variation varied between 0.3 to 2.26. Callus induced with 2.4-D showed 86 % stability, and with picloram was 64 %. The callus formation was more evident after application of 2.4 D. This is the first study of embryogenic potential in cells of the torch ginger, and showed that 2,4-D induced callus with higher starch content and genetically stable.
id UFLA_1e6da96505cd84d5c5a8c11e698c34a4
oai_identifier_str oai:localhost:1/38379
network_acronym_str UFLA
network_name_str Repositório Institucional da UFLA
repository_id_str
spelling Embryogenic induction of torch ginger (Etlingera elatior) callusInducción embriogénica en callo de jengibre antorcha (Etlingera elatior)ZingiberaceaeEtlingera elatiorViabilityViabilidadTorch ginger Etlingera elatior (Jack) R.M. Smith is a tropical herb-like, rhizome-like and perennial plant that possesses inflorescences that are both pretty and showy at different colour shades, and have a great visual appeal. Producing seedlings at a high enough level when done conventionally by the division of tussocks, has high costs and phytosanitary problems. The objective of this study was to evaluate the induction of somatic embryogenesis and its potential for subsequent plant regeneration in torch ginger. Rhizomes, leaves and roots from the seedlings grown in vitro were used as explant segments. The different explants were cultured on MS medium supplemented with 30 g L-1 sucrose, and it was solidified with 5.5 g L-1 of agar. The effect of 2.4-D and picloram concentrations of 0.0, 1.0, 2.0 and 4.0 mg L-1 was studied. Astructural analysis was performed by scanning electron microscopy (SEM). Cytochemical analysis were performed to detect nucleeous with carmine acetic, starch with lugol and lipids with Sudan III, and a flow cytometry analysis to verify the stability of ploidy in the callus. Results indicate that the culture medium with 2.4-D yielded the induction of embryogenic callus with the characteristics of the rhizome segments of the explant. Regarding the ultrastructural analysis of embryogenic callus characteristics, the cells showed a similar isodiametric form as somatic embryos in the globular stage and cytochemical analysis. The presence of callus cells in pro-embryogenic explants containing each of the regulators was confirmed. In the cytometry analysis, the average DNA content of callus ranged from 5.43 to 10.15 pg, and the coefficients of variation varied between 0.3 to 2.26. Callus induced with 2.4-D showed 86 % stability, and with picloram was 64 %. The callus formation was more evident after application of 2.4 D. This is the first study of embryogenic potential in cells of the torch ginger, and showed that 2,4-D induced callus with higher starch content and genetically stable.El jengibre antorcha Etlingera elatior (Jack) R. M. Smith es una planta tropical perenne, similar al rizoma, que tiene inflorescencias que son bellas y llamativas, de diferentes colores y matices y de un gran atractivo visual. La producción de plántulas en un nivel lo suficientemente alto cuando se hace convencionalmente utilizando la división de matas tiene altos costos y problemas fitosanitarios. El objetivo de este estudio fue evaluar la inducción de la embriogénesis somática y su uso potencial para la reproducción posterior de plantas de jengibre antorcha. Los rizomas, hojas y raíces de las plántulas cultivadas in vitro se utilizaron como segmentos de explantes. Los diferentes explantes se cultivaron en un medio MS al que se le agregaron 30 g L-1 de sacarosa y fue solidificado con 5.5 g de agar L-1. Se estudió el efecto del 2.4-D y picloram en concentraciones de 0.0, 1.0, 2.0 y 4.0 mg L-1. El análisis estructural se llevó a cabo por microscopía electrónica de barrido (SEM). Se realizaron análisis citoquímicos para detectar núcleos con carmín acético, almidón con lugol y lípidos con Sudán III; y un análisis de citometría de flujo para verificar la estabilidad de la ploidía en el callo. Los resultados indicaron que el medio de cultivo con 2.4-D produjo la inducción de callo embriogénico con las características de los segmentos de rizomas del explante. En el análisis ultraestructural de las características del callo embriogénico, las células mostraron una forma isodiamétrica similar a embriones somáticos en etapa globular y análisis citoquímico. Se confirmó la presencia de células de callo en explantes pro- embriogénicos conteniendo cada uno de los reguladores. En el análisis de citometría, el contenido medio de ADN del callo varió de 5.43 a 10.15 pg, y los coeficientes de variación oscilaron entre 0.3 y 2.26. El 2.4-D indujo callos con estabilidad de 86 %, y con picloram fue 64 %. La formación de callo fue más evidente después de aplicar 2.4-D. Éste es el primer estudio del potencial embriogénico de células del jengibre antorcha, y mostró que el 2.4-D indujo la formación de callo con mayor contenido de almidón y genéticamente estable.Colegio de Postgraduados2019-12-30T18:18:42Z2019-12-30T18:18:42Z2014-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfGOMES-DÍAS, G. de M. et al. Embryogenic induction of torch ginger (Etlingera elatior) callus. Agrociencia, Texcoco, v. 48, n. 2, p. 173-184, Feb./Mar. 2014.http://repositorio.ufla.br/jspui/handle/1/38379Agrocienciareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessGomes-Dias, Gabrielen de M.Almendagna-Rodrigues, FilipeRodrigues-Soares, DóriaPasqual, MoacirCarvalho, A. Cristina Portugal-Pinto deeng2023-05-26T18:42:15Zoai:localhost:1/38379Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:42:15Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Embryogenic induction of torch ginger (Etlingera elatior) callus
Inducción embriogénica en callo de jengibre antorcha (Etlingera elatior)
title Embryogenic induction of torch ginger (Etlingera elatior) callus
spellingShingle Embryogenic induction of torch ginger (Etlingera elatior) callus
Gomes-Dias, Gabrielen de M.
Zingiberaceae
Etlingera elatior
Viability
Viabilidad
title_short Embryogenic induction of torch ginger (Etlingera elatior) callus
title_full Embryogenic induction of torch ginger (Etlingera elatior) callus
title_fullStr Embryogenic induction of torch ginger (Etlingera elatior) callus
title_full_unstemmed Embryogenic induction of torch ginger (Etlingera elatior) callus
title_sort Embryogenic induction of torch ginger (Etlingera elatior) callus
author Gomes-Dias, Gabrielen de M.
author_facet Gomes-Dias, Gabrielen de M.
Almendagna-Rodrigues, Filipe
Rodrigues-Soares, Dória
Pasqual, Moacir
Carvalho, A. Cristina Portugal-Pinto de
author_role author
author2 Almendagna-Rodrigues, Filipe
Rodrigues-Soares, Dória
Pasqual, Moacir
Carvalho, A. Cristina Portugal-Pinto de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Gomes-Dias, Gabrielen de M.
Almendagna-Rodrigues, Filipe
Rodrigues-Soares, Dória
Pasqual, Moacir
Carvalho, A. Cristina Portugal-Pinto de
dc.subject.por.fl_str_mv Zingiberaceae
Etlingera elatior
Viability
Viabilidad
topic Zingiberaceae
Etlingera elatior
Viability
Viabilidad
description Torch ginger Etlingera elatior (Jack) R.M. Smith is a tropical herb-like, rhizome-like and perennial plant that possesses inflorescences that are both pretty and showy at different colour shades, and have a great visual appeal. Producing seedlings at a high enough level when done conventionally by the division of tussocks, has high costs and phytosanitary problems. The objective of this study was to evaluate the induction of somatic embryogenesis and its potential for subsequent plant regeneration in torch ginger. Rhizomes, leaves and roots from the seedlings grown in vitro were used as explant segments. The different explants were cultured on MS medium supplemented with 30 g L-1 sucrose, and it was solidified with 5.5 g L-1 of agar. The effect of 2.4-D and picloram concentrations of 0.0, 1.0, 2.0 and 4.0 mg L-1 was studied. Astructural analysis was performed by scanning electron microscopy (SEM). Cytochemical analysis were performed to detect nucleeous with carmine acetic, starch with lugol and lipids with Sudan III, and a flow cytometry analysis to verify the stability of ploidy in the callus. Results indicate that the culture medium with 2.4-D yielded the induction of embryogenic callus with the characteristics of the rhizome segments of the explant. Regarding the ultrastructural analysis of embryogenic callus characteristics, the cells showed a similar isodiametric form as somatic embryos in the globular stage and cytochemical analysis. The presence of callus cells in pro-embryogenic explants containing each of the regulators was confirmed. In the cytometry analysis, the average DNA content of callus ranged from 5.43 to 10.15 pg, and the coefficients of variation varied between 0.3 to 2.26. Callus induced with 2.4-D showed 86 % stability, and with picloram was 64 %. The callus formation was more evident after application of 2.4 D. This is the first study of embryogenic potential in cells of the torch ginger, and showed that 2,4-D induced callus with higher starch content and genetically stable.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
2019-12-30T18:18:42Z
2019-12-30T18:18:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv GOMES-DÍAS, G. de M. et al. Embryogenic induction of torch ginger (Etlingera elatior) callus. Agrociencia, Texcoco, v. 48, n. 2, p. 173-184, Feb./Mar. 2014.
http://repositorio.ufla.br/jspui/handle/1/38379
identifier_str_mv GOMES-DÍAS, G. de M. et al. Embryogenic induction of torch ginger (Etlingera elatior) callus. Agrociencia, Texcoco, v. 48, n. 2, p. 173-184, Feb./Mar. 2014.
url http://repositorio.ufla.br/jspui/handle/1/38379
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Colegio de Postgraduados
publisher.none.fl_str_mv Colegio de Postgraduados
dc.source.none.fl_str_mv Agrociencia
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1815439064997822464