Physiological performance and expression of isozymes in maize seeds subjected to water stress
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/28284 |
Resumo: | Early characterization of genotypes, through the previous assessment of physiological quality of their seeds and their enzymatic systems, can provide parameters capable of aiding in the selection of new maize cultivars tolerant to water stress. Thus, this study was aimed at assessing seed performance of five different maize lines subjected to four different water stress levels. The physiological quality of seeds germinated under the osmotic potentials of 0.0, -0.3, -0.6 and -0.9 MPa was assessed by germination test, number of strong normal seedlings and T50, as well as by the expression of the isozymes superoxide dismutase (SOD), catalase (CAT), glutamate oxaloacetate transaminase (GOT), esterase (EST), malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), and of heat-resistant proteins. The osmotic potential of -0.9 MPa affected the physiological quality and vigor of seeds of all maize lines assessed. The highest values for the germination percent, in the lowest osmotic potentials assessed, were found in the seeds of line 63. However, seeds of line 91 were superior in regard to vigor. Increased expression of the enzymes SOD and CAT, considered as enzymes of the antioxidant system, was observed in seeds of lines 44 and 91. Thus, line 91 was considered as promising for tolerance to water stress. |
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Physiological performance and expression of isozymes in maize seeds subjected to water stressDesempenho fisiológico e expressão de isoenzimas em sementes de milho submetidas ao estresse hídricoPhysiological qualityWater deficitDrought toleranceQualidade fisiológicaDéficit hídricoTolerância à secaEarly characterization of genotypes, through the previous assessment of physiological quality of their seeds and their enzymatic systems, can provide parameters capable of aiding in the selection of new maize cultivars tolerant to water stress. Thus, this study was aimed at assessing seed performance of five different maize lines subjected to four different water stress levels. The physiological quality of seeds germinated under the osmotic potentials of 0.0, -0.3, -0.6 and -0.9 MPa was assessed by germination test, number of strong normal seedlings and T50, as well as by the expression of the isozymes superoxide dismutase (SOD), catalase (CAT), glutamate oxaloacetate transaminase (GOT), esterase (EST), malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), and of heat-resistant proteins. The osmotic potential of -0.9 MPa affected the physiological quality and vigor of seeds of all maize lines assessed. The highest values for the germination percent, in the lowest osmotic potentials assessed, were found in the seeds of line 63. However, seeds of line 91 were superior in regard to vigor. Increased expression of the enzymes SOD and CAT, considered as enzymes of the antioxidant system, was observed in seeds of lines 44 and 91. Thus, line 91 was considered as promising for tolerance to water stress.A caracterização precoce de genótipos, por meio de avaliação prévia da qualidade fisiológica de suas sementes e seus sistemas enzimáticos, pode fornecer parâmetros capazes de auxiliar na seleção de novas cultivares de milho tolerantes ao estresse hídrico. Assim, o objetivo deste estudo foi avaliar o desempenho das sementes de cinco linhagens de milho submetidas à quatro níveis de estresse hídrico. Para isso, a qualidade fisiológica das sementes, germinadas sob os potenciais osmóticos de 0,0; -0,3; -0,6 e -0,9 MPa, foi avaliada pelo teste de germinação, número de plântulas normais fortes e T50, bem como pela expressão das isoenzimas superóxido dismutase (SOD), catalase (CAT), glutamato oxalacetato transaminase (GOT), esterase (EST), malato desidrogenase (MDH), álcool desidrogenase (ADH) e de proteínas resistentes ao calor. O potencial osmótico de -0,9 MPa afetou a qualidade fisiológica, bem como o vigor das sementes de todas as linhagens avaliadas. Os maiores valores para a porcentagem de germinação, nos menores potenciais osmóticos avaliados, foram observados nas sementes da linhagem 63. No entanto, sementes da linhagem 91 foram superiores quanto ao vigor. Maior expressão das enzimas SOD e CAT, consideradas como enzimas do sistema antioxidante, foram observadas em sementes das linhagens 44 e 91. Assim, a linhagem 91 é considerada como linhagem promissora quanto à tolerância ao estresse hídrico.Associação Brasileira de Tecnologia de Sementes2017-12-18T12:27:24Z2017-12-18T12:27:24Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfABREU, V. M. de et al. Physiological performance and expression of isozymes in maize seeds subjected to water stress. Journal of Seed Science, Londrina, v. 36, n. 1, p. 40-47, 2014.http://repositorio.ufla.br/jspui/handle/1/28284Journal of Seed Sciencereponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessAbreu, Viviane Maria deVon Pinho, Édila Vilela de ResendeVon Pinho, Renzo GarciaNaves, Glória Maria de FreitasSilva Neta, Izabel CostaGuimarães, Renato MendesCarvalho, Marcela Rezende deeng2023-05-26T18:42:36Zoai:localhost:1/28284Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:42:36Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Physiological performance and expression of isozymes in maize seeds subjected to water stress Desempenho fisiológico e expressão de isoenzimas em sementes de milho submetidas ao estresse hídrico |
title |
Physiological performance and expression of isozymes in maize seeds subjected to water stress |
spellingShingle |
Physiological performance and expression of isozymes in maize seeds subjected to water stress Abreu, Viviane Maria de Physiological quality Water deficit Drought tolerance Qualidade fisiológica Déficit hídrico Tolerância à seca |
title_short |
Physiological performance and expression of isozymes in maize seeds subjected to water stress |
title_full |
Physiological performance and expression of isozymes in maize seeds subjected to water stress |
title_fullStr |
Physiological performance and expression of isozymes in maize seeds subjected to water stress |
title_full_unstemmed |
Physiological performance and expression of isozymes in maize seeds subjected to water stress |
title_sort |
Physiological performance and expression of isozymes in maize seeds subjected to water stress |
author |
Abreu, Viviane Maria de |
author_facet |
Abreu, Viviane Maria de Von Pinho, Édila Vilela de Resende Von Pinho, Renzo Garcia Naves, Glória Maria de Freitas Silva Neta, Izabel Costa Guimarães, Renato Mendes Carvalho, Marcela Rezende de |
author_role |
author |
author2 |
Von Pinho, Édila Vilela de Resende Von Pinho, Renzo Garcia Naves, Glória Maria de Freitas Silva Neta, Izabel Costa Guimarães, Renato Mendes Carvalho, Marcela Rezende de |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Abreu, Viviane Maria de Von Pinho, Édila Vilela de Resende Von Pinho, Renzo Garcia Naves, Glória Maria de Freitas Silva Neta, Izabel Costa Guimarães, Renato Mendes Carvalho, Marcela Rezende de |
dc.subject.por.fl_str_mv |
Physiological quality Water deficit Drought tolerance Qualidade fisiológica Déficit hídrico Tolerância à seca |
topic |
Physiological quality Water deficit Drought tolerance Qualidade fisiológica Déficit hídrico Tolerância à seca |
description |
Early characterization of genotypes, through the previous assessment of physiological quality of their seeds and their enzymatic systems, can provide parameters capable of aiding in the selection of new maize cultivars tolerant to water stress. Thus, this study was aimed at assessing seed performance of five different maize lines subjected to four different water stress levels. The physiological quality of seeds germinated under the osmotic potentials of 0.0, -0.3, -0.6 and -0.9 MPa was assessed by germination test, number of strong normal seedlings and T50, as well as by the expression of the isozymes superoxide dismutase (SOD), catalase (CAT), glutamate oxaloacetate transaminase (GOT), esterase (EST), malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), and of heat-resistant proteins. The osmotic potential of -0.9 MPa affected the physiological quality and vigor of seeds of all maize lines assessed. The highest values for the germination percent, in the lowest osmotic potentials assessed, were found in the seeds of line 63. However, seeds of line 91 were superior in regard to vigor. Increased expression of the enzymes SOD and CAT, considered as enzymes of the antioxidant system, was observed in seeds of lines 44 and 91. Thus, line 91 was considered as promising for tolerance to water stress. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2017-12-18T12:27:24Z 2017-12-18T12:27:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
ABREU, V. M. de et al. Physiological performance and expression of isozymes in maize seeds subjected to water stress. Journal of Seed Science, Londrina, v. 36, n. 1, p. 40-47, 2014. http://repositorio.ufla.br/jspui/handle/1/28284 |
identifier_str_mv |
ABREU, V. M. de et al. Physiological performance and expression of isozymes in maize seeds subjected to water stress. Journal of Seed Science, Londrina, v. 36, n. 1, p. 40-47, 2014. |
url |
http://repositorio.ufla.br/jspui/handle/1/28284 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Tecnologia de Sementes |
publisher.none.fl_str_mv |
Associação Brasileira de Tecnologia de Sementes |
dc.source.none.fl_str_mv |
Journal of Seed Science reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835155198377984 |