Micropropagation of Maclura tinctoria L.: an endangered woody species

Detalhes bibliográficos
Autor(a) principal: Gomes, Guilherme Augusto Canella
Data de Publicação: 2010
Outros Autores: Paiva, Renato, Herrera, Rairys Cravo, Paiva, Patrícia Duarte de Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/38731
Resumo: Some native species produce seeds with low germination percentage and in most cases with dormancy, which makes the appearance of new individuals by sexual propagation difficult. The Maclura tinctoria has been considered an endangered species due to the indiscriminate use of its wood and low rate of seed germination. In this context, the objective of the present study was to establish an in vitropropagation methodology for this species. Combinations of NAA + BAP, different concentrations of GA3 and combinations IBA + activated charcoal were evaluated for shoot induction, shoot growth and root formation, respectively. The results indicated that the maximum shoot formation was obtained when 5.37 µM NAA + 4.45 µM BAP was used. The use of >5.48 µM GA3 promoted shoot growth. Root formation was observed on explants inoculated in WPM with a pH adjusted to 7.0 and supplemented with 23.62 µM IBA + 4.7 g L-1 activated charcoal. The use of a 70% light screen for 7 days followed by the use of 50 and 30% light screens also for 7 days each provided 97% plantlet survival.
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spelling Micropropagation of Maclura tinctoria L.: an endangered woody speciesMicropropagação de Maclura Tinctoria L.: uma espécie lenhosa em extinçãoMaclura tinctoriaPropagation and acclimatizationPropagação e aclimatizaçãoSome native species produce seeds with low germination percentage and in most cases with dormancy, which makes the appearance of new individuals by sexual propagation difficult. The Maclura tinctoria has been considered an endangered species due to the indiscriminate use of its wood and low rate of seed germination. In this context, the objective of the present study was to establish an in vitropropagation methodology for this species. Combinations of NAA + BAP, different concentrations of GA3 and combinations IBA + activated charcoal were evaluated for shoot induction, shoot growth and root formation, respectively. The results indicated that the maximum shoot formation was obtained when 5.37 µM NAA + 4.45 µM BAP was used. The use of >5.48 µM GA3 promoted shoot growth. Root formation was observed on explants inoculated in WPM with a pH adjusted to 7.0 and supplemented with 23.62 µM IBA + 4.7 g L-1 activated charcoal. The use of a 70% light screen for 7 days followed by the use of 50 and 30% light screens also for 7 days each provided 97% plantlet survival.Algumas espécies nativas produzem sementes com baixa porcentagem de germinação e, na maioria dos casos, dormência que pode dificultar o aparecimento de novos indivíduos, por meio da propagação sexuada. A Maclura tinctoria tem sido considerada como ameaçada de extinção devido ao uso indiscriminado de sua madeira e à baixa taxa de germinação de suas sementes. Nesse contexto, o objetivo deste estudo foi estabelecer uma metodologia de propagação in vitro para a espécie. Combinações de ANA + BAP, diferentes concentrações de GA3 e combinações de AIB + carvão ativado foram avaliadas na indução de brotações, alongamento caulinar e indução de enraizamento, respectivamente. Os resultados indicaram que a máxima formação de brotações foi obtida quando 5,37 mM NAA + 4,45 mM BAP foram utilizados. O crescimento das brotações foi observado com 5,48 mM GA3. Para a formação de raízes, foi indicado o uso do meio WPM, com pH ajustado para 7,0, suplementado com 23,62 mM AIB e 4,7 g L-1 de carvão ativado. O uso de sombrite 70% por sete dias, seguido da utilização de sombrite 50 e 30%, também por sete dias cada, promoveu 97% de sobrevivência de plantas.Sociedade de Investigações Florestais (SIF)2020-01-27T18:36:46Z2020-01-27T18:36:46Z2010-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfGOMES, G. A. C. et al. Micropropagation of Maclura tinctoria L.: an endangered woody species. Revista Árvore, Viçosa, MG, v. 34, n. 1, p. 25-30, 2010. DOI: 10.1590/S0100-67622010000100003.http://repositorio.ufla.br/jspui/handle/1/38731Revista Árvorereponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessGomes, Guilherme Augusto CanellaPaiva, RenatoHerrera, Rairys CravoPaiva, Patrícia Duarte de Oliveiraeng2023-05-26T18:51:38Zoai:localhost:1/38731Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:51:38Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Micropropagation of Maclura tinctoria L.: an endangered woody species
Micropropagação de Maclura Tinctoria L.: uma espécie lenhosa em extinção
title Micropropagation of Maclura tinctoria L.: an endangered woody species
spellingShingle Micropropagation of Maclura tinctoria L.: an endangered woody species
Gomes, Guilherme Augusto Canella
Maclura tinctoria
Propagation and acclimatization
Propagação e aclimatização
title_short Micropropagation of Maclura tinctoria L.: an endangered woody species
title_full Micropropagation of Maclura tinctoria L.: an endangered woody species
title_fullStr Micropropagation of Maclura tinctoria L.: an endangered woody species
title_full_unstemmed Micropropagation of Maclura tinctoria L.: an endangered woody species
title_sort Micropropagation of Maclura tinctoria L.: an endangered woody species
author Gomes, Guilherme Augusto Canella
author_facet Gomes, Guilherme Augusto Canella
Paiva, Renato
Herrera, Rairys Cravo
Paiva, Patrícia Duarte de Oliveira
author_role author
author2 Paiva, Renato
Herrera, Rairys Cravo
Paiva, Patrícia Duarte de Oliveira
author2_role author
author
author
dc.contributor.author.fl_str_mv Gomes, Guilherme Augusto Canella
Paiva, Renato
Herrera, Rairys Cravo
Paiva, Patrícia Duarte de Oliveira
dc.subject.por.fl_str_mv Maclura tinctoria
Propagation and acclimatization
Propagação e aclimatização
topic Maclura tinctoria
Propagation and acclimatization
Propagação e aclimatização
description Some native species produce seeds with low germination percentage and in most cases with dormancy, which makes the appearance of new individuals by sexual propagation difficult. The Maclura tinctoria has been considered an endangered species due to the indiscriminate use of its wood and low rate of seed germination. In this context, the objective of the present study was to establish an in vitropropagation methodology for this species. Combinations of NAA + BAP, different concentrations of GA3 and combinations IBA + activated charcoal were evaluated for shoot induction, shoot growth and root formation, respectively. The results indicated that the maximum shoot formation was obtained when 5.37 µM NAA + 4.45 µM BAP was used. The use of >5.48 µM GA3 promoted shoot growth. Root formation was observed on explants inoculated in WPM with a pH adjusted to 7.0 and supplemented with 23.62 µM IBA + 4.7 g L-1 activated charcoal. The use of a 70% light screen for 7 days followed by the use of 50 and 30% light screens also for 7 days each provided 97% plantlet survival.
publishDate 2010
dc.date.none.fl_str_mv 2010-02
2020-01-27T18:36:46Z
2020-01-27T18:36:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv GOMES, G. A. C. et al. Micropropagation of Maclura tinctoria L.: an endangered woody species. Revista Árvore, Viçosa, MG, v. 34, n. 1, p. 25-30, 2010. DOI: 10.1590/S0100-67622010000100003.
http://repositorio.ufla.br/jspui/handle/1/38731
identifier_str_mv GOMES, G. A. C. et al. Micropropagation of Maclura tinctoria L.: an endangered woody species. Revista Árvore, Viçosa, MG, v. 34, n. 1, p. 25-30, 2010. DOI: 10.1590/S0100-67622010000100003.
url http://repositorio.ufla.br/jspui/handle/1/38731
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade de Investigações Florestais (SIF)
publisher.none.fl_str_mv Sociedade de Investigações Florestais (SIF)
dc.source.none.fl_str_mv Revista Árvore
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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