Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy

Detalhes bibliográficos
Autor(a) principal: Silva, Daiani M.
Data de Publicação: 2011
Outros Autores: Batista, Luís R., Rezende, Elisângela F., Fungaro, Maria Helena P., Sartori, Daniele, Alves, Eduardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/9714
Resumo: In spite of the taxonomy of the Aspergillus species of the Nigri Section being regarded as troublesome, a number of methods have been proposed to aid in the classification of this Section. This work aimed to distinguish Aspergillus species of the Nigri Section from foods, grains and caves on the basis in Polyphasic Taxonomy by utilizing morphologic and physiologic characters, and sequencing of ß-tubulin and calmodulin genes. The morphologic identification proved useful for some species, such as A. carbonarius and Aspergillus sp UFLA DCA 01, despite not having been totally effective in elucidating species related to A. niger. The isolation of the species of the Nigri Section on Creatine Sucrose Agar (CREA) enabled to distinguish the Aspergillus sp species, which was characterized by the lack of sporulation and by the production of sclerotia. Scanning Electron microscopy (SEM) allowed distinguishing the species into two distinct groups. The production of Ochratoxin A (OTA) was only found in the A. carbonarius and A. niger species. The sequencing of -tubulin gene was efficient in differing most of the Aspergillus species from the Nigri Section with the exception of Aspergillus UFLA DCA 01, which could not be distinguished from A. costaricaensis. This species is morphologically similar to A. costaricaencis for its low sporulation capacity and high sclerotia production, but it differs morphologically from A. costaricaensis for its conidial ornamentation and size of vesicles. Equally, based on partial calmodulin gene sequence data Aspergillus UFLA DCA 01 differs from A. costaricaensis.
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spelling Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomyPolyphasic taxonomyβ-tubulin geneAspergillus spp morphologyIn spite of the taxonomy of the Aspergillus species of the Nigri Section being regarded as troublesome, a number of methods have been proposed to aid in the classification of this Section. This work aimed to distinguish Aspergillus species of the Nigri Section from foods, grains and caves on the basis in Polyphasic Taxonomy by utilizing morphologic and physiologic characters, and sequencing of ß-tubulin and calmodulin genes. The morphologic identification proved useful for some species, such as A. carbonarius and Aspergillus sp UFLA DCA 01, despite not having been totally effective in elucidating species related to A. niger. The isolation of the species of the Nigri Section on Creatine Sucrose Agar (CREA) enabled to distinguish the Aspergillus sp species, which was characterized by the lack of sporulation and by the production of sclerotia. Scanning Electron microscopy (SEM) allowed distinguishing the species into two distinct groups. The production of Ochratoxin A (OTA) was only found in the A. carbonarius and A. niger species. The sequencing of -tubulin gene was efficient in differing most of the Aspergillus species from the Nigri Section with the exception of Aspergillus UFLA DCA 01, which could not be distinguished from A. costaricaensis. This species is morphologically similar to A. costaricaencis for its low sporulation capacity and high sclerotia production, but it differs morphologically from A. costaricaensis for its conidial ornamentation and size of vesicles. Equally, based on partial calmodulin gene sequence data Aspergillus UFLA DCA 01 differs from A. costaricaensis.Sociedade Brasileira de Microbiologia2015-07-24T17:49:59Z2015-07-24T17:49:59Z2011-01-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSILVA, D. M. et al. Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy. Brazilian Journal of Microbiology, São Paulo, v. 42, n. 2, p. 761-773, Apr. 2011.http://repositorio.ufla.br/jspui/handle/1/9714Brazilian Journal of Microbiologyreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLASilva, Daiani M.Batista, Luís R.Rezende, Elisângela F.Fungaro, Maria Helena P.Sartori, DanieleAlves, Eduardoinfo:eu-repo/semantics/openAccesseng2023-05-26T19:52:48Zoai:localhost:1/9714Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T19:52:48Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy
title Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy
spellingShingle Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy
Silva, Daiani M.
Polyphasic taxonomy
β-tubulin gene
Aspergillus spp morphology
title_short Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy
title_full Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy
title_fullStr Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy
title_full_unstemmed Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy
title_sort Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy
author Silva, Daiani M.
author_facet Silva, Daiani M.
Batista, Luís R.
Rezende, Elisângela F.
Fungaro, Maria Helena P.
Sartori, Daniele
Alves, Eduardo
author_role author
author2 Batista, Luís R.
Rezende, Elisângela F.
Fungaro, Maria Helena P.
Sartori, Daniele
Alves, Eduardo
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Daiani M.
Batista, Luís R.
Rezende, Elisângela F.
Fungaro, Maria Helena P.
Sartori, Daniele
Alves, Eduardo
dc.subject.por.fl_str_mv Polyphasic taxonomy
β-tubulin gene
Aspergillus spp morphology
topic Polyphasic taxonomy
β-tubulin gene
Aspergillus spp morphology
description In spite of the taxonomy of the Aspergillus species of the Nigri Section being regarded as troublesome, a number of methods have been proposed to aid in the classification of this Section. This work aimed to distinguish Aspergillus species of the Nigri Section from foods, grains and caves on the basis in Polyphasic Taxonomy by utilizing morphologic and physiologic characters, and sequencing of ß-tubulin and calmodulin genes. The morphologic identification proved useful for some species, such as A. carbonarius and Aspergillus sp UFLA DCA 01, despite not having been totally effective in elucidating species related to A. niger. The isolation of the species of the Nigri Section on Creatine Sucrose Agar (CREA) enabled to distinguish the Aspergillus sp species, which was characterized by the lack of sporulation and by the production of sclerotia. Scanning Electron microscopy (SEM) allowed distinguishing the species into two distinct groups. The production of Ochratoxin A (OTA) was only found in the A. carbonarius and A. niger species. The sequencing of -tubulin gene was efficient in differing most of the Aspergillus species from the Nigri Section with the exception of Aspergillus UFLA DCA 01, which could not be distinguished from A. costaricaensis. This species is morphologically similar to A. costaricaencis for its low sporulation capacity and high sclerotia production, but it differs morphologically from A. costaricaensis for its conidial ornamentation and size of vesicles. Equally, based on partial calmodulin gene sequence data Aspergillus UFLA DCA 01 differs from A. costaricaensis.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-13
2015-07-24T17:49:59Z
2015-07-24T17:49:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv SILVA, D. M. et al. Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy. Brazilian Journal of Microbiology, São Paulo, v. 42, n. 2, p. 761-773, Apr. 2011.
http://repositorio.ufla.br/jspui/handle/1/9714
identifier_str_mv SILVA, D. M. et al. Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy. Brazilian Journal of Microbiology, São Paulo, v. 42, n. 2, p. 761-773, Apr. 2011.
url http://repositorio.ufla.br/jspui/handle/1/9714
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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