Culturable diazotrophic bacterial community associated with Agave sisalana P. plants from semi-arid regions in Brazil

Detalhes bibliográficos
Autor(a) principal: Santos, Adailson Feitoza de Jesus
Data de Publicação: 2019
Outros Autores: Moreira, Zayda Piedad Morales, Souza, Jorge Teodoro de, Oliveira, Lenaldo Muniz de, Barbosa, Heloiza Ramos, Silva, Eliane de Souza, Silva, Rafael Mota da, Soares, Ana Cristina Fermino
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/40573
Resumo: The aim of this study was to isolate, quantify, identify and characterize the culturable diazotrophic bacterial community associated with sisal (Agave sisalana P.) and assess its potential for plant growth promotion and tolerance to abiotic stress. Nitrogen-free Burk’s medium was used for bacteria isolation and quantification. Isolates carrying the nifH gene were typed by BOX-PCR and sequenced for the 16S rRNA region. Additionally, the bacteria was tested for growth promotion in maize plants and for salinity tolerance in cucumber seedlings. The representative isolates were identified as members of Pantoea, Rhizobium, Burkholderia, Leifsonia and Bacillus genera. All isolates were positive for at least two of the nine plant growth promoting (PGP) traits tested. All isolates showed positive traits for tolerance to abiotic stress. Leifsonia sp. (S1.5) significantly increased root length, stem length and total fresh matter, and it was involved in the reduction of deleterious salt effects (60 mM NaCl) in cucumber seedlings. Diazotrophic bacterial community associated with sisal plants is genetically diverse, exhibits several plant growth-promoting traits and shows potential to support plant growth in adverse environments (salinity).
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spelling Culturable diazotrophic bacterial community associated with Agave sisalana P. plants from semi-arid regions in BrazilComunidade bacteriana diazotrófica cultivável associada com Agave sisalana P. de regiões semi-áridas do BrasilAbiotic stressPlant growthRhizospheric bacteriaEstresse abitóticoPlantas - CrescimentoBactéria rizosféricaThe aim of this study was to isolate, quantify, identify and characterize the culturable diazotrophic bacterial community associated with sisal (Agave sisalana P.) and assess its potential for plant growth promotion and tolerance to abiotic stress. Nitrogen-free Burk’s medium was used for bacteria isolation and quantification. Isolates carrying the nifH gene were typed by BOX-PCR and sequenced for the 16S rRNA region. Additionally, the bacteria was tested for growth promotion in maize plants and for salinity tolerance in cucumber seedlings. The representative isolates were identified as members of Pantoea, Rhizobium, Burkholderia, Leifsonia and Bacillus genera. All isolates were positive for at least two of the nine plant growth promoting (PGP) traits tested. All isolates showed positive traits for tolerance to abiotic stress. Leifsonia sp. (S1.5) significantly increased root length, stem length and total fresh matter, and it was involved in the reduction of deleterious salt effects (60 mM NaCl) in cucumber seedlings. Diazotrophic bacterial community associated with sisal plants is genetically diverse, exhibits several plant growth-promoting traits and shows potential to support plant growth in adverse environments (salinity).O objetivo deste estudo foi isolar, quantificar, identificar e caracterizar a comunidade bacteriana diazotrófica cultivável associada com sisal (Agave sisalana P.) e avaliar o seu potencial para promoção de crescimento vegetal e tolerância a estresse abiótico. Para isolamento e quantificação das bactérias foi utilizado o meio de cultura Burk’s livre de nitrogênio. Isolados contendo o gene nifH foram caracterizados usando BOX-PCR e em seguida a região 16S rRNA foi sequenciada. As bactérias foram testadas para promoção de crescimento em plantas de milho e para indução de tolerância a salinidade em mudas de pepino. Os isolados representativos foram identificados como membros dos gêneros Pantoea, Rhizobium, Burkholderia, Leifsonia e Bacillus. Todos os isolados foram positivos para pelo menos duas das nove caractetísticas de promoção de crescimento vegetal (PCV) testadas. Todos os isolados apresentaram características positivas para tolerância a estresse abiótico. Leifsonia sp. (S1.5) aumentou significativamente o comprimento da raíz, caule e matéria fresca total e reduziu os efeitos deletérios do sal (60 mM) nas mudas de pepino. A comunidade bacteriana diazotrófica associada com plantas de sisal é geneticamente diversa, possui diversas características para promoção de crescimento e apresenta potencial para manter o cresicmento de plantas em condições adversas (salinidade).Universidade Federal Rural de Pernambuco2020-05-05T12:33:09Z2020-05-05T12:33:09Z2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSANTOS, A. F. de J. et al. Culturable diazotrophic bacterial community associated with Agave sisalana P. plants from semi-arid regions in Brazil. Revista Brasileira de Ciências Agrárias, Recife, v. 14, n. 3, 2019. Paginação irregular.http://repositorio.ufla.br/jspui/handle/1/40573Revista Brasileira de Ciências Agráriasreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessSantos, Adailson Feitoza de JesusMoreira, Zayda Piedad MoralesSouza, Jorge Teodoro deOliveira, Lenaldo Muniz deBarbosa, Heloiza RamosSilva, Eliane de SouzaSilva, Rafael Mota daSoares, Ana Cristina Ferminoeng2020-05-05T12:35:55Zoai:localhost:1/40573Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-05-05T12:35:55Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Culturable diazotrophic bacterial community associated with Agave sisalana P. plants from semi-arid regions in Brazil
Comunidade bacteriana diazotrófica cultivável associada com Agave sisalana P. de regiões semi-áridas do Brasil
title Culturable diazotrophic bacterial community associated with Agave sisalana P. plants from semi-arid regions in Brazil
spellingShingle Culturable diazotrophic bacterial community associated with Agave sisalana P. plants from semi-arid regions in Brazil
Santos, Adailson Feitoza de Jesus
Abiotic stress
Plant growth
Rhizospheric bacteria
Estresse abitótico
Plantas - Crescimento
Bactéria rizosférica
title_short Culturable diazotrophic bacterial community associated with Agave sisalana P. plants from semi-arid regions in Brazil
title_full Culturable diazotrophic bacterial community associated with Agave sisalana P. plants from semi-arid regions in Brazil
title_fullStr Culturable diazotrophic bacterial community associated with Agave sisalana P. plants from semi-arid regions in Brazil
title_full_unstemmed Culturable diazotrophic bacterial community associated with Agave sisalana P. plants from semi-arid regions in Brazil
title_sort Culturable diazotrophic bacterial community associated with Agave sisalana P. plants from semi-arid regions in Brazil
author Santos, Adailson Feitoza de Jesus
author_facet Santos, Adailson Feitoza de Jesus
Moreira, Zayda Piedad Morales
Souza, Jorge Teodoro de
Oliveira, Lenaldo Muniz de
Barbosa, Heloiza Ramos
Silva, Eliane de Souza
Silva, Rafael Mota da
Soares, Ana Cristina Fermino
author_role author
author2 Moreira, Zayda Piedad Morales
Souza, Jorge Teodoro de
Oliveira, Lenaldo Muniz de
Barbosa, Heloiza Ramos
Silva, Eliane de Souza
Silva, Rafael Mota da
Soares, Ana Cristina Fermino
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Adailson Feitoza de Jesus
Moreira, Zayda Piedad Morales
Souza, Jorge Teodoro de
Oliveira, Lenaldo Muniz de
Barbosa, Heloiza Ramos
Silva, Eliane de Souza
Silva, Rafael Mota da
Soares, Ana Cristina Fermino
dc.subject.por.fl_str_mv Abiotic stress
Plant growth
Rhizospheric bacteria
Estresse abitótico
Plantas - Crescimento
Bactéria rizosférica
topic Abiotic stress
Plant growth
Rhizospheric bacteria
Estresse abitótico
Plantas - Crescimento
Bactéria rizosférica
description The aim of this study was to isolate, quantify, identify and characterize the culturable diazotrophic bacterial community associated with sisal (Agave sisalana P.) and assess its potential for plant growth promotion and tolerance to abiotic stress. Nitrogen-free Burk’s medium was used for bacteria isolation and quantification. Isolates carrying the nifH gene were typed by BOX-PCR and sequenced for the 16S rRNA region. Additionally, the bacteria was tested for growth promotion in maize plants and for salinity tolerance in cucumber seedlings. The representative isolates were identified as members of Pantoea, Rhizobium, Burkholderia, Leifsonia and Bacillus genera. All isolates were positive for at least two of the nine plant growth promoting (PGP) traits tested. All isolates showed positive traits for tolerance to abiotic stress. Leifsonia sp. (S1.5) significantly increased root length, stem length and total fresh matter, and it was involved in the reduction of deleterious salt effects (60 mM NaCl) in cucumber seedlings. Diazotrophic bacterial community associated with sisal plants is genetically diverse, exhibits several plant growth-promoting traits and shows potential to support plant growth in adverse environments (salinity).
publishDate 2019
dc.date.none.fl_str_mv 2019
2020-05-05T12:33:09Z
2020-05-05T12:33:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv SANTOS, A. F. de J. et al. Culturable diazotrophic bacterial community associated with Agave sisalana P. plants from semi-arid regions in Brazil. Revista Brasileira de Ciências Agrárias, Recife, v. 14, n. 3, 2019. Paginação irregular.
http://repositorio.ufla.br/jspui/handle/1/40573
identifier_str_mv SANTOS, A. F. de J. et al. Culturable diazotrophic bacterial community associated with Agave sisalana P. plants from semi-arid regions in Brazil. Revista Brasileira de Ciências Agrárias, Recife, v. 14, n. 3, 2019. Paginação irregular.
url http://repositorio.ufla.br/jspui/handle/1/40573
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
dc.source.none.fl_str_mv Revista Brasileira de Ciências Agrárias
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
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institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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