Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/33620 |
Resumo: | This study aimed to isolate and identify endophytic fungi from the forage grass P. maximum and evaluate their ability to inhibit the growth of plant pathogenic fungi. One sample from P. purpureum grass was also included. Surface disinfected stem fragments were used for endophytic fungal isolation. One hundred and twenty-six endophytic fungi were isolated, of which 118 were from P. maximum and eight from P. purpureum. Morphological characteristics and internal transcribed spacer (ITS) and 18S (NS) sequence comparisons identified most isolated endophytic fungi as belonging to the phylum Ascomycota, with Sarocladium being the dominant genus. The isolates were subjected to in vitro antagonism tests against pathogenic fungi, and 31 endophytic fungi inhibited the growth of Bipolaris maydis, Penicillium expansum, and Sclerotinia minor. The results expand our knowledge of the diversity of endophytes associated with tropical grasses and suggest that they may represent new sources of antifungal metabolites for biocontrol and biotechnological purposes. |
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Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potentialAntagonismGrassesMolecular identificationPlant pathogensrDNAThis study aimed to isolate and identify endophytic fungi from the forage grass P. maximum and evaluate their ability to inhibit the growth of plant pathogenic fungi. One sample from P. purpureum grass was also included. Surface disinfected stem fragments were used for endophytic fungal isolation. One hundred and twenty-six endophytic fungi were isolated, of which 118 were from P. maximum and eight from P. purpureum. Morphological characteristics and internal transcribed spacer (ITS) and 18S (NS) sequence comparisons identified most isolated endophytic fungi as belonging to the phylum Ascomycota, with Sarocladium being the dominant genus. The isolates were subjected to in vitro antagonism tests against pathogenic fungi, and 31 endophytic fungi inhibited the growth of Bipolaris maydis, Penicillium expansum, and Sclerotinia minor. The results expand our knowledge of the diversity of endophytes associated with tropical grasses and suggest that they may represent new sources of antifungal metabolites for biocontrol and biotechnological purposes.Universidade Federal de Viçosa (UFV), Departamento de Zootecnia (DZO)2019-04-17T21:03:56Z2019-04-17T21:03:56Z2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMAIA, N. da C. et al. Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential. Revista Brasileira de Zootecnia, Viçosa, MG, v. 47, p. 1-6, 2018. DOI: 10.1590/rbz4720170183.http://repositorio.ufla.br/jspui/handle/1/33620Revista Brasileira de Zootecnia (RBZ)reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessMaia, Natália da CostaSouza, Patrícia Nirlane da CostaGodinho, Bárbara Temponi VilarinoMoreira, Silvino IntraAbreu, Lucas Magalhães deJank, LianaCardoso, Patrícia Gomeseng2019-04-17T21:03:56Zoai:localhost:1/33620Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-04-17T21:03:56Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential |
title |
Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential |
spellingShingle |
Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential Maia, Natália da Costa Antagonism Grasses Molecular identification Plant pathogens rDNA |
title_short |
Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential |
title_full |
Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential |
title_fullStr |
Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential |
title_full_unstemmed |
Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential |
title_sort |
Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential |
author |
Maia, Natália da Costa |
author_facet |
Maia, Natália da Costa Souza, Patrícia Nirlane da Costa Godinho, Bárbara Temponi Vilarino Moreira, Silvino Intra Abreu, Lucas Magalhães de Jank, Liana Cardoso, Patrícia Gomes |
author_role |
author |
author2 |
Souza, Patrícia Nirlane da Costa Godinho, Bárbara Temponi Vilarino Moreira, Silvino Intra Abreu, Lucas Magalhães de Jank, Liana Cardoso, Patrícia Gomes |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Maia, Natália da Costa Souza, Patrícia Nirlane da Costa Godinho, Bárbara Temponi Vilarino Moreira, Silvino Intra Abreu, Lucas Magalhães de Jank, Liana Cardoso, Patrícia Gomes |
dc.subject.por.fl_str_mv |
Antagonism Grasses Molecular identification Plant pathogens rDNA |
topic |
Antagonism Grasses Molecular identification Plant pathogens rDNA |
description |
This study aimed to isolate and identify endophytic fungi from the forage grass P. maximum and evaluate their ability to inhibit the growth of plant pathogenic fungi. One sample from P. purpureum grass was also included. Surface disinfected stem fragments were used for endophytic fungal isolation. One hundred and twenty-six endophytic fungi were isolated, of which 118 were from P. maximum and eight from P. purpureum. Morphological characteristics and internal transcribed spacer (ITS) and 18S (NS) sequence comparisons identified most isolated endophytic fungi as belonging to the phylum Ascomycota, with Sarocladium being the dominant genus. The isolates were subjected to in vitro antagonism tests against pathogenic fungi, and 31 endophytic fungi inhibited the growth of Bipolaris maydis, Penicillium expansum, and Sclerotinia minor. The results expand our knowledge of the diversity of endophytes associated with tropical grasses and suggest that they may represent new sources of antifungal metabolites for biocontrol and biotechnological purposes. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2019-04-17T21:03:56Z 2019-04-17T21:03:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
MAIA, N. da C. et al. Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential. Revista Brasileira de Zootecnia, Viçosa, MG, v. 47, p. 1-6, 2018. DOI: 10.1590/rbz4720170183. http://repositorio.ufla.br/jspui/handle/1/33620 |
identifier_str_mv |
MAIA, N. da C. et al. Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential. Revista Brasileira de Zootecnia, Viçosa, MG, v. 47, p. 1-6, 2018. DOI: 10.1590/rbz4720170183. |
url |
http://repositorio.ufla.br/jspui/handle/1/33620 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Viçosa (UFV), Departamento de Zootecnia (DZO) |
publisher.none.fl_str_mv |
Universidade Federal de Viçosa (UFV), Departamento de Zootecnia (DZO) |
dc.source.none.fl_str_mv |
Revista Brasileira de Zootecnia (RBZ) reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1784550092035850240 |