Preliminary results for genetic transformation of shoot tip of Eucalyptus saligna Sm. via Agrobacterium tumefaciens
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/37285 |
Resumo: | The regeneration of transgenic plants of Eucalypt is the largest difficulty for the genetic transformation of this genus; in addition a low rate of transformed plants is reached. The aim of this research was to evaluate acetosyringone (3',5'-Dimethoxy-4'-hydroxyacetophenone) on the co-culture medium during genetic transformation of shoot tips of Eucalyptus saligna via Agrobacterium tumefaciens and to promote the explants selection supposedly transformed. Shoot tip from multiple shoots was used as explants. These explants were pre-cultured during two days before the transformation. Strain EHA105 of A. tumefaciens harboring the plasmid pBI121 was used. The treatments were: 0 and 100 μM acetosyringone added to the co-culture, after co-culture the explants were cultured in multiplication medium supplemented with 250 mg.L-1 Cefotaxime® and each sub-culture the kanamycin levels were increased from 50 to 150 mg.L-1. The transient expression of the uidA gene in shoot tips was evaluated after the end of the co-culture (fifth day) and after seven days of culture on medium containing kanamycin. The presence of 100 μM acetosyringone at the co-culture of shoot tips of Eucalyptus saligna promoted higher transient expression of the uidA gene and retards toxic effects caused by kanamycin. |
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Preliminary results for genetic transformation of shoot tip of Eucalyptus saligna Sm. via Agrobacterium tumefaciensStrain EHA105GUS geneEucalyptβ-glucuronidaseThe regeneration of transgenic plants of Eucalypt is the largest difficulty for the genetic transformation of this genus; in addition a low rate of transformed plants is reached. The aim of this research was to evaluate acetosyringone (3',5'-Dimethoxy-4'-hydroxyacetophenone) on the co-culture medium during genetic transformation of shoot tips of Eucalyptus saligna via Agrobacterium tumefaciens and to promote the explants selection supposedly transformed. Shoot tip from multiple shoots was used as explants. These explants were pre-cultured during two days before the transformation. Strain EHA105 of A. tumefaciens harboring the plasmid pBI121 was used. The treatments were: 0 and 100 μM acetosyringone added to the co-culture, after co-culture the explants were cultured in multiplication medium supplemented with 250 mg.L-1 Cefotaxime® and each sub-culture the kanamycin levels were increased from 50 to 150 mg.L-1. The transient expression of the uidA gene in shoot tips was evaluated after the end of the co-culture (fifth day) and after seven days of culture on medium containing kanamycin. The presence of 100 μM acetosyringone at the co-culture of shoot tips of Eucalyptus saligna promoted higher transient expression of the uidA gene and retards toxic effects caused by kanamycin.A regeneração de plantas transgênicas de eucalipto representa a maior dificuldade para a transformação genética de plantas do gênero Eucalyptus, além de que taxas baixas de plantas transformadas são alcançadas. O objetivo desse trabalho foi avaliar a acetosiringona no meio de co-cultura durante a transformação genética de ápices caulinares de Eucalyptus saligna via Agrobacterium tumefaciens e promover a seleção dos supostos explantes transformados. Ápices caulinares originados de brotações múltiplas foram usados como explantes. Estes explantes foram pré-cultivados por dois dias antes da transformação. A linhagem EHA105 de A. tumefaciens contendo o plasmídeo pBI120 foi utilizada. Os tratamentos foram: 0 e 100 μM de acetosiringona adicionada ao meio de co-cultura, após a co-cultura os explantes foram cultivados em meio de multiplicação suplementado com 250 mg.L-1 de cefotaxima e a cada subcultivo a concentração de canamicina foi aumentada, de 50 até 150 mg.L-1. A expressão transiente do gene uidA nos ápices caulinares foi avaliada no final da co-cultura e após sete dias em meio com canamicina (agente seletivo). A presença de 100 μM de acetosiringona no meio de co-cultura dos ápices caulinares de Eucalyptus saligna promoveu maior expressão transiente do gene uidA e retardou a ação tóxica causada pela canamicina.Universidade Federal do Tocantins2019-10-17T18:48:38Z2019-10-17T18:48:38Z2011-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSILVA, A. L. L. da et al. Preliminary results for genetic transformation of shoot tip of Eucalyptus saligna Sm. via Agrobacterium tumefaciens. Journal of Biotechnology and Biodiversity, [S.l.], v. 2, n. 1, p. 1-6, Feb. 2011.http://repositorio.ufla.br/jspui/handle/1/37285Journal of Biotechnology and Biodiversityreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessSilva, André Luis Lopes daOliveira, YohanaCosta, Jefferson da LuzMudry, Clarissa de SouzaScheidt, Gessiel NewtonBrondani, Gilvano Eblingeng2019-10-17T18:49:30Zoai:localhost:1/37285Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-10-17T18:49:30Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Preliminary results for genetic transformation of shoot tip of Eucalyptus saligna Sm. via Agrobacterium tumefaciens |
title |
Preliminary results for genetic transformation of shoot tip of Eucalyptus saligna Sm. via Agrobacterium tumefaciens |
spellingShingle |
Preliminary results for genetic transformation of shoot tip of Eucalyptus saligna Sm. via Agrobacterium tumefaciens Silva, André Luis Lopes da Strain EHA105 GUS gene Eucalypt β-glucuronidase |
title_short |
Preliminary results for genetic transformation of shoot tip of Eucalyptus saligna Sm. via Agrobacterium tumefaciens |
title_full |
Preliminary results for genetic transformation of shoot tip of Eucalyptus saligna Sm. via Agrobacterium tumefaciens |
title_fullStr |
Preliminary results for genetic transformation of shoot tip of Eucalyptus saligna Sm. via Agrobacterium tumefaciens |
title_full_unstemmed |
Preliminary results for genetic transformation of shoot tip of Eucalyptus saligna Sm. via Agrobacterium tumefaciens |
title_sort |
Preliminary results for genetic transformation of shoot tip of Eucalyptus saligna Sm. via Agrobacterium tumefaciens |
author |
Silva, André Luis Lopes da |
author_facet |
Silva, André Luis Lopes da Oliveira, Yohana Costa, Jefferson da Luz Mudry, Clarissa de Souza Scheidt, Gessiel Newton Brondani, Gilvano Ebling |
author_role |
author |
author2 |
Oliveira, Yohana Costa, Jefferson da Luz Mudry, Clarissa de Souza Scheidt, Gessiel Newton Brondani, Gilvano Ebling |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Silva, André Luis Lopes da Oliveira, Yohana Costa, Jefferson da Luz Mudry, Clarissa de Souza Scheidt, Gessiel Newton Brondani, Gilvano Ebling |
dc.subject.por.fl_str_mv |
Strain EHA105 GUS gene Eucalypt β-glucuronidase |
topic |
Strain EHA105 GUS gene Eucalypt β-glucuronidase |
description |
The regeneration of transgenic plants of Eucalypt is the largest difficulty for the genetic transformation of this genus; in addition a low rate of transformed plants is reached. The aim of this research was to evaluate acetosyringone (3',5'-Dimethoxy-4'-hydroxyacetophenone) on the co-culture medium during genetic transformation of shoot tips of Eucalyptus saligna via Agrobacterium tumefaciens and to promote the explants selection supposedly transformed. Shoot tip from multiple shoots was used as explants. These explants were pre-cultured during two days before the transformation. Strain EHA105 of A. tumefaciens harboring the plasmid pBI121 was used. The treatments were: 0 and 100 μM acetosyringone added to the co-culture, after co-culture the explants were cultured in multiplication medium supplemented with 250 mg.L-1 Cefotaxime® and each sub-culture the kanamycin levels were increased from 50 to 150 mg.L-1. The transient expression of the uidA gene in shoot tips was evaluated after the end of the co-culture (fifth day) and after seven days of culture on medium containing kanamycin. The presence of 100 μM acetosyringone at the co-culture of shoot tips of Eucalyptus saligna promoted higher transient expression of the uidA gene and retards toxic effects caused by kanamycin. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-02 2019-10-17T18:48:38Z 2019-10-17T18:48:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SILVA, A. L. L. da et al. Preliminary results for genetic transformation of shoot tip of Eucalyptus saligna Sm. via Agrobacterium tumefaciens. Journal of Biotechnology and Biodiversity, [S.l.], v. 2, n. 1, p. 1-6, Feb. 2011. http://repositorio.ufla.br/jspui/handle/1/37285 |
identifier_str_mv |
SILVA, A. L. L. da et al. Preliminary results for genetic transformation of shoot tip of Eucalyptus saligna Sm. via Agrobacterium tumefaciens. Journal of Biotechnology and Biodiversity, [S.l.], v. 2, n. 1, p. 1-6, Feb. 2011. |
url |
http://repositorio.ufla.br/jspui/handle/1/37285 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal do Tocantins |
publisher.none.fl_str_mv |
Universidade Federal do Tocantins |
dc.source.none.fl_str_mv |
Journal of Biotechnology and Biodiversity reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1823242081074675712 |