Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee

Detalhes bibliográficos
Autor(a) principal: Pereira, Gabriella Santos
Data de Publicação: 2011
Outros Autores: Padilha, Lilian, Von Pinho, Edila Vilela Resende, Teixeira, Rita de Kássia Siqueira, Carvalho, Carlos Henrique Siqueira de, Maluf, Mirian Peres, Carvalho, Bruna Line de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/28243
Resumo: The objective of this work was to analyze coffee (Coffea arabica) genotypes resistant to the coffee leaf miner (Leucoptera coffeella) using microsatellite markers. Sixty-six loci were evaluated, of which 63 were obtained from the Brazilian Coffee Expressed Sequence Tag (EST) database. These loci were amplified in bulks of individuals from F5 progenies of 'Siriema' (C. arabica x C. racemosa) resistant and susceptible to the insect, in eight samples of C. racemosa, and in a F6 population of 'Siriema' with 91 individuals segregating for resistance to the leaf miner. Polymorphisms were verified for two simple sequence repeat (SSR) loci in bulks of the susceptible progenies. The two polymorphic alleles were present in around 70% of the susceptible genotypes in F5 and in approximately 90% of the susceptible individuals in F6. However, the polymorphic EST-SSR markers among populations contrasting for resistance to leaf miner were not correlated to the evaluated characteristics. SSR markers show inter- and intraspecific polymorphism in C. arabica and C. racemosa.
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spelling Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffeeMarcadores microssatélites na análise de resistência ao bicho-mineiro em café arábicaCoffea arabica L.Leucoptera coffeellaEST-SSRGenotypesGenótiposThe objective of this work was to analyze coffee (Coffea arabica) genotypes resistant to the coffee leaf miner (Leucoptera coffeella) using microsatellite markers. Sixty-six loci were evaluated, of which 63 were obtained from the Brazilian Coffee Expressed Sequence Tag (EST) database. These loci were amplified in bulks of individuals from F5 progenies of 'Siriema' (C. arabica x C. racemosa) resistant and susceptible to the insect, in eight samples of C. racemosa, and in a F6 population of 'Siriema' with 91 individuals segregating for resistance to the leaf miner. Polymorphisms were verified for two simple sequence repeat (SSR) loci in bulks of the susceptible progenies. The two polymorphic alleles were present in around 70% of the susceptible genotypes in F5 and in approximately 90% of the susceptible individuals in F6. However, the polymorphic EST-SSR markers among populations contrasting for resistance to leaf miner were not correlated to the evaluated characteristics. SSR markers show inter- and intraspecific polymorphism in C. arabica and C. racemosa.O objetivo deste trabalho foi analisar genótipos de cafeeiro (Coffea arabica) resistentes ao bicho-mineiro (Leucoptera coffeella), com uso de marcadores microssatélites. Foram avaliados 66 locos, dos quais 63 foram obtidos do banco de sequências expressas (EST) de café. Esses locos foram amplificados em "bulks" de progênies F5 de 'Siriema' (C. arabica x C. racemosa) resistentes e suscetíveis ao ataque da praga, em oito acessos de C. racemosa, e em uma população F6 de 'Siriema' com 91 indivíduos que segregam para resistência ao bicho-mineiro. Os polimorfismos foram verificados em dois locos de marcadores microssatélites (SSR), nos "bulks" das progênies suscetíveis. Os dois alelos polimórficos estiveram presentes em torno de 70% dos genótipos suscetíveis em F5 e em, aproximadamente, 90% dos indivíduos suscetíveis em F6. Entretanto, os marcadores EST-SSR polimórficos entre populações contrastantes para a resistência ao bicho-mineiro não foram correlacionados às características avaliadas. Os marcadores SSR apresentam polimorfismo inter e intraespecífico em C. arabica e C. racemosa.Embrapa Informação Tecnológica Pesquisa Agropecuária Brasileira2017-12-12T12:31:56Z2017-12-12T12:31:56Z2011-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfPEREIRA, G. S. et al. Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 46, n. 12, Dec. 2011.http://repositorio.ufla.br/jspui/handle/1/28243Pesquisa Agropecuária Brasileirareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessPereira, Gabriella SantosPadilha, LilianVon Pinho, Edila Vilela ResendeTeixeira, Rita de Kássia SiqueiraCarvalho, Carlos Henrique Siqueira deMaluf, Mirian PeresCarvalho, Bruna Line depor2017-12-12T12:31:57Zoai:localhost:1/28243Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2017-12-12T12:31:57Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee
Marcadores microssatélites na análise de resistência ao bicho-mineiro em café arábica
title Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee
spellingShingle Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee
Pereira, Gabriella Santos
Coffea arabica L.
Leucoptera coffeella
EST-SSR
Genotypes
Genótipos
title_short Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee
title_full Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee
title_fullStr Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee
title_full_unstemmed Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee
title_sort Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee
author Pereira, Gabriella Santos
author_facet Pereira, Gabriella Santos
Padilha, Lilian
Von Pinho, Edila Vilela Resende
Teixeira, Rita de Kássia Siqueira
Carvalho, Carlos Henrique Siqueira de
Maluf, Mirian Peres
Carvalho, Bruna Line de
author_role author
author2 Padilha, Lilian
Von Pinho, Edila Vilela Resende
Teixeira, Rita de Kássia Siqueira
Carvalho, Carlos Henrique Siqueira de
Maluf, Mirian Peres
Carvalho, Bruna Line de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira, Gabriella Santos
Padilha, Lilian
Von Pinho, Edila Vilela Resende
Teixeira, Rita de Kássia Siqueira
Carvalho, Carlos Henrique Siqueira de
Maluf, Mirian Peres
Carvalho, Bruna Line de
dc.subject.por.fl_str_mv Coffea arabica L.
Leucoptera coffeella
EST-SSR
Genotypes
Genótipos
topic Coffea arabica L.
Leucoptera coffeella
EST-SSR
Genotypes
Genótipos
description The objective of this work was to analyze coffee (Coffea arabica) genotypes resistant to the coffee leaf miner (Leucoptera coffeella) using microsatellite markers. Sixty-six loci were evaluated, of which 63 were obtained from the Brazilian Coffee Expressed Sequence Tag (EST) database. These loci were amplified in bulks of individuals from F5 progenies of 'Siriema' (C. arabica x C. racemosa) resistant and susceptible to the insect, in eight samples of C. racemosa, and in a F6 population of 'Siriema' with 91 individuals segregating for resistance to the leaf miner. Polymorphisms were verified for two simple sequence repeat (SSR) loci in bulks of the susceptible progenies. The two polymorphic alleles were present in around 70% of the susceptible genotypes in F5 and in approximately 90% of the susceptible individuals in F6. However, the polymorphic EST-SSR markers among populations contrasting for resistance to leaf miner were not correlated to the evaluated characteristics. SSR markers show inter- and intraspecific polymorphism in C. arabica and C. racemosa.
publishDate 2011
dc.date.none.fl_str_mv 2011-12
2017-12-12T12:31:56Z
2017-12-12T12:31:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv PEREIRA, G. S. et al. Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 46, n. 12, Dec. 2011.
http://repositorio.ufla.br/jspui/handle/1/28243
identifier_str_mv PEREIRA, G. S. et al. Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 46, n. 12, Dec. 2011.
url http://repositorio.ufla.br/jspui/handle/1/28243
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Embrapa Informação Tecnológica Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Embrapa Informação Tecnológica Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuária Brasileira
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1815438948985470976

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