Optimization of culture conditions for tannase production by Aspergillus sp. GM4 in solid state fermentation

Detalhes bibliográficos
Autor(a) principal: Souza, Patrícia Nirlane da Costa
Data de Publicação: 2015
Outros Autores: Maia, Natália da Costa, Guimarães, Luís Henrique Souza, Resende, Mário Lúcio Vilela de, Cardoso, Patrícia Gomes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/39935
Resumo: Tannase is an industrially important enzyme produced by a large number of microorganisms. This study analyzed the production of tannase by Aspergillus sp. GM4 under solid-state fermentation (SSF) using different vegetable leaves (mango, jamun and coffee) and agricultural residues (coffee husks, rice husks and wheat bran). Among the substrates used jamun leaves yielded high tannase production. The Plackett-Burman design was conducted to evaluate the effects of 12 independent variables on the production of tannase under SSF using jamun leaves as substrate. Among these variables, incubation time, potassium nitrate and tannic acid had significant effects on enzyme production. A lower incubation time was fixed and supplementation with potassium nitrate and tannic acid were optimized using the Central Composite Design. The best conditions for tannase production were: incubation time of 2 days; tannic acid at 1.53% (w w-1) and potassium nitrate at 2.71% (w w-1). After the optimization process, tannase production increased 4.65-fold, which showed that the statistical experimental design offers a practicable approach to the implementation of optimization of tannase production.
id UFLA_2b6b8050262662f526821348ce308240
oai_identifier_str oai:localhost:1/39935
network_acronym_str UFLA
network_name_str Repositório Institucional da UFLA
repository_id_str
spelling Optimization of culture conditions for tannase production by Aspergillus sp. GM4 in solid state fermentationOtimização das condições de cultivo para produção de tanase por Aspergillus sp. GM4 em fermentação em estado sólidoSurface response methodologyEnzymeJamunAgricultural residuesMetodologia de superfície de respostaEnzimaJambolãoResíduos agrícolasTannase is an industrially important enzyme produced by a large number of microorganisms. This study analyzed the production of tannase by Aspergillus sp. GM4 under solid-state fermentation (SSF) using different vegetable leaves (mango, jamun and coffee) and agricultural residues (coffee husks, rice husks and wheat bran). Among the substrates used jamun leaves yielded high tannase production. The Plackett-Burman design was conducted to evaluate the effects of 12 independent variables on the production of tannase under SSF using jamun leaves as substrate. Among these variables, incubation time, potassium nitrate and tannic acid had significant effects on enzyme production. A lower incubation time was fixed and supplementation with potassium nitrate and tannic acid were optimized using the Central Composite Design. The best conditions for tannase production were: incubation time of 2 days; tannic acid at 1.53% (w w-1) and potassium nitrate at 2.71% (w w-1). After the optimization process, tannase production increased 4.65-fold, which showed that the statistical experimental design offers a practicable approach to the implementation of optimization of tannase production.Tanase é uma enzima industrialmente importante produzida por um grande número de microrganismos. Este estudo analisou a produção de tanase por Aspergillus sp. GM4 em fermentação em estado sólido (FES) utilizando diferentes vegetais como folhas de manga, de jambolão, de café e resíduos agrícolas, como a casca de café, casca de arroz e farelo de trigo. Entre os substratos utilizados, as folhas jambolão renderam alta produção de tanase. O planejamento de Plackett-Burman foi conduzido para avaliar os efeitos de 12 variáveis independentes sobre a produção de tanase em FES usando folhas jambolão como substrato. Entre estas variáveis, tiveram efeitos significativos na produção da enzima o tempo de incubação, o nitrato de potássio e o ácido tânico. O menor tempo de incubação foi fixado e a suplementação de nitrato de potássio e ácido tânico foi otimizada utilizando o planejamento composto central rotacional. As melhores condições para a produção de tanase foram o tempo de incubação de dois dias, a concentração de ácido tânico de 1,53% (g g-1) e de nitrato de potássio 2,71% (g gw-1). Após o processo de otimização, a produção tanase aumentou 4,65 vezes, o que mostrou que o delineamento experimental foi um método viável para a otimização da produção de tanase.Editora da Universidade Estadual de Maringá (EDUEM)2020-04-09T21:11:12Z2020-04-09T21:11:12Z2015-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSOUZA, P. N. da C. et al. Optimization of culture conditions for tannase production by Aspergillus sp. GM4 in solid state fermentation. Acta Scientiarum. Biological Sciences, Maringá, v. 37, n. 1, p. 23-30, Jan./Mar. 2015. DOI: 10.4025/actascibiolsci.v37i1.22731.http://repositorio.ufla.br/jspui/handle/1/39935Acta Scientiarum. Biological Sciencesreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessSouza, Patrícia Nirlane da CostaMaia, Natália da CostaGuimarães, Luís Henrique SouzaResende, Mário Lúcio Vilela deCardoso, Patrícia Gomeseng2020-04-09T21:11:12Zoai:localhost:1/39935Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-04-09T21:11:12Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Optimization of culture conditions for tannase production by Aspergillus sp. GM4 in solid state fermentation
Otimização das condições de cultivo para produção de tanase por Aspergillus sp. GM4 em fermentação em estado sólido
title Optimization of culture conditions for tannase production by Aspergillus sp. GM4 in solid state fermentation
spellingShingle Optimization of culture conditions for tannase production by Aspergillus sp. GM4 in solid state fermentation
Souza, Patrícia Nirlane da Costa
Surface response methodology
Enzyme
Jamun
Agricultural residues
Metodologia de superfície de resposta
Enzima
Jambolão
Resíduos agrícolas
title_short Optimization of culture conditions for tannase production by Aspergillus sp. GM4 in solid state fermentation
title_full Optimization of culture conditions for tannase production by Aspergillus sp. GM4 in solid state fermentation
title_fullStr Optimization of culture conditions for tannase production by Aspergillus sp. GM4 in solid state fermentation
title_full_unstemmed Optimization of culture conditions for tannase production by Aspergillus sp. GM4 in solid state fermentation
title_sort Optimization of culture conditions for tannase production by Aspergillus sp. GM4 in solid state fermentation
author Souza, Patrícia Nirlane da Costa
author_facet Souza, Patrícia Nirlane da Costa
Maia, Natália da Costa
Guimarães, Luís Henrique Souza
Resende, Mário Lúcio Vilela de
Cardoso, Patrícia Gomes
author_role author
author2 Maia, Natália da Costa
Guimarães, Luís Henrique Souza
Resende, Mário Lúcio Vilela de
Cardoso, Patrícia Gomes
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Souza, Patrícia Nirlane da Costa
Maia, Natália da Costa
Guimarães, Luís Henrique Souza
Resende, Mário Lúcio Vilela de
Cardoso, Patrícia Gomes
dc.subject.por.fl_str_mv Surface response methodology
Enzyme
Jamun
Agricultural residues
Metodologia de superfície de resposta
Enzima
Jambolão
Resíduos agrícolas
topic Surface response methodology
Enzyme
Jamun
Agricultural residues
Metodologia de superfície de resposta
Enzima
Jambolão
Resíduos agrícolas
description Tannase is an industrially important enzyme produced by a large number of microorganisms. This study analyzed the production of tannase by Aspergillus sp. GM4 under solid-state fermentation (SSF) using different vegetable leaves (mango, jamun and coffee) and agricultural residues (coffee husks, rice husks and wheat bran). Among the substrates used jamun leaves yielded high tannase production. The Plackett-Burman design was conducted to evaluate the effects of 12 independent variables on the production of tannase under SSF using jamun leaves as substrate. Among these variables, incubation time, potassium nitrate and tannic acid had significant effects on enzyme production. A lower incubation time was fixed and supplementation with potassium nitrate and tannic acid were optimized using the Central Composite Design. The best conditions for tannase production were: incubation time of 2 days; tannic acid at 1.53% (w w-1) and potassium nitrate at 2.71% (w w-1). After the optimization process, tannase production increased 4.65-fold, which showed that the statistical experimental design offers a practicable approach to the implementation of optimization of tannase production.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
2020-04-09T21:11:12Z
2020-04-09T21:11:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv SOUZA, P. N. da C. et al. Optimization of culture conditions for tannase production by Aspergillus sp. GM4 in solid state fermentation. Acta Scientiarum. Biological Sciences, Maringá, v. 37, n. 1, p. 23-30, Jan./Mar. 2015. DOI: 10.4025/actascibiolsci.v37i1.22731.
http://repositorio.ufla.br/jspui/handle/1/39935
identifier_str_mv SOUZA, P. N. da C. et al. Optimization of culture conditions for tannase production by Aspergillus sp. GM4 in solid state fermentation. Acta Scientiarum. Biological Sciences, Maringá, v. 37, n. 1, p. 23-30, Jan./Mar. 2015. DOI: 10.4025/actascibiolsci.v37i1.22731.
url http://repositorio.ufla.br/jspui/handle/1/39935
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Editora da Universidade Estadual de Maringá (EDUEM)
publisher.none.fl_str_mv Editora da Universidade Estadual de Maringá (EDUEM)
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1823242048007831552

Registros relacionados