Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro

Detalhes bibliográficos
Autor(a) principal: Müller, Alexandra
Data de Publicação: 2017
Outros Autores: Schneider, Jannis F., Degrossoli, Adriana, Lupilova, Nataliya, Dick, Tobias P., Leichert, Lars I.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/29313
Resumo: This article contains representative fluorescence excitation spectra of roGFP2-based probes used for ratiometric analysis of redox changes as presented in the article "Systematic in vitro assessment of responses of roGFP2-based probes to physiologically relevant oxidant species". The recombinant probes roGFP2, roGFP2-Orp1, and Grx1-roGFP2 were exposed to various oxidative and nitrosative species, including hydrogen peroxide (H2O2), aldrithiol-2 (AT-2), glutathione disulfide (GSSG), hypochlorous acid (HOCl), S-nitrosoglutathione (GSNO), peroxynitrite (ONOO−), potassium polysulfide (K2Sx), spermine NONOate (SperNO), and diethyl amino NONOate (DeaNO) at different molar ratios. Fluorescence excitation spectra of the probes were recorded in the excitation wavelength range between 350 and 500 nm and for a total of 60 min. Analysis and interpretation of the data is presented in an associated article.
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spelling Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitroGenetically encoded redox probesPolysulfidesHypochlorous acid (HOCl)PeroxynitriteGlutathioneHydrogen peroxide (H2O2)NitricoxideThis article contains representative fluorescence excitation spectra of roGFP2-based probes used for ratiometric analysis of redox changes as presented in the article "Systematic in vitro assessment of responses of roGFP2-based probes to physiologically relevant oxidant species". The recombinant probes roGFP2, roGFP2-Orp1, and Grx1-roGFP2 were exposed to various oxidative and nitrosative species, including hydrogen peroxide (H2O2), aldrithiol-2 (AT-2), glutathione disulfide (GSSG), hypochlorous acid (HOCl), S-nitrosoglutathione (GSNO), peroxynitrite (ONOO−), potassium polysulfide (K2Sx), spermine NONOate (SperNO), and diethyl amino NONOate (DeaNO) at different molar ratios. Fluorescence excitation spectra of the probes were recorded in the excitation wavelength range between 350 and 500 nm and for a total of 60 min. Analysis and interpretation of the data is presented in an associated article.Elsevier2018-05-25T11:22:15Z2018-05-25T11:22:15Z2017-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMÜLLER, A. et al. Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro. Data in Brief, [S.l.], v. 11, p. 617-627, Apr. 2017.http://repositorio.ufla.br/jspui/handle/1/29313Data in Briefreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessMüller, AlexandraSchneider, Jannis F.Degrossoli, AdrianaLupilova, NataliyaDick, Tobias P.Leichert, Lars I.eng2018-05-25T11:23:36Zoai:localhost:1/29313Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2018-05-25T11:23:36Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro
title Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro
spellingShingle Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro
Müller, Alexandra
Genetically encoded redox probes
Polysulfides
Hypochlorous acid (HOCl)
Peroxynitrite
Glutathione
Hydrogen peroxide (H2O2)
Nitricoxide
title_short Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro
title_full Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro
title_fullStr Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro
title_full_unstemmed Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro
title_sort Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro
author Müller, Alexandra
author_facet Müller, Alexandra
Schneider, Jannis F.
Degrossoli, Adriana
Lupilova, Nataliya
Dick, Tobias P.
Leichert, Lars I.
author_role author
author2 Schneider, Jannis F.
Degrossoli, Adriana
Lupilova, Nataliya
Dick, Tobias P.
Leichert, Lars I.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Müller, Alexandra
Schneider, Jannis F.
Degrossoli, Adriana
Lupilova, Nataliya
Dick, Tobias P.
Leichert, Lars I.
dc.subject.por.fl_str_mv Genetically encoded redox probes
Polysulfides
Hypochlorous acid (HOCl)
Peroxynitrite
Glutathione
Hydrogen peroxide (H2O2)
Nitricoxide
topic Genetically encoded redox probes
Polysulfides
Hypochlorous acid (HOCl)
Peroxynitrite
Glutathione
Hydrogen peroxide (H2O2)
Nitricoxide
description This article contains representative fluorescence excitation spectra of roGFP2-based probes used for ratiometric analysis of redox changes as presented in the article "Systematic in vitro assessment of responses of roGFP2-based probes to physiologically relevant oxidant species". The recombinant probes roGFP2, roGFP2-Orp1, and Grx1-roGFP2 were exposed to various oxidative and nitrosative species, including hydrogen peroxide (H2O2), aldrithiol-2 (AT-2), glutathione disulfide (GSSG), hypochlorous acid (HOCl), S-nitrosoglutathione (GSNO), peroxynitrite (ONOO−), potassium polysulfide (K2Sx), spermine NONOate (SperNO), and diethyl amino NONOate (DeaNO) at different molar ratios. Fluorescence excitation spectra of the probes were recorded in the excitation wavelength range between 350 and 500 nm and for a total of 60 min. Analysis and interpretation of the data is presented in an associated article.
publishDate 2017
dc.date.none.fl_str_mv 2017-04
2018-05-25T11:22:15Z
2018-05-25T11:22:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv MÜLLER, A. et al. Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro. Data in Brief, [S.l.], v. 11, p. 617-627, Apr. 2017.
http://repositorio.ufla.br/jspui/handle/1/29313
identifier_str_mv MÜLLER, A. et al. Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro. Data in Brief, [S.l.], v. 11, p. 617-627, Apr. 2017.
url http://repositorio.ufla.br/jspui/handle/1/29313
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Data in Brief
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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