Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/29313 |
Resumo: | This article contains representative fluorescence excitation spectra of roGFP2-based probes used for ratiometric analysis of redox changes as presented in the article "Systematic in vitro assessment of responses of roGFP2-based probes to physiologically relevant oxidant species". The recombinant probes roGFP2, roGFP2-Orp1, and Grx1-roGFP2 were exposed to various oxidative and nitrosative species, including hydrogen peroxide (H2O2), aldrithiol-2 (AT-2), glutathione disulfide (GSSG), hypochlorous acid (HOCl), S-nitrosoglutathione (GSNO), peroxynitrite (ONOO−), potassium polysulfide (K2Sx), spermine NONOate (SperNO), and diethyl amino NONOate (DeaNO) at different molar ratios. Fluorescence excitation spectra of the probes were recorded in the excitation wavelength range between 350 and 500 nm and for a total of 60 min. Analysis and interpretation of the data is presented in an associated article. |
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Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitroGenetically encoded redox probesPolysulfidesHypochlorous acid (HOCl)PeroxynitriteGlutathioneHydrogen peroxide (H2O2)NitricoxideThis article contains representative fluorescence excitation spectra of roGFP2-based probes used for ratiometric analysis of redox changes as presented in the article "Systematic in vitro assessment of responses of roGFP2-based probes to physiologically relevant oxidant species". The recombinant probes roGFP2, roGFP2-Orp1, and Grx1-roGFP2 were exposed to various oxidative and nitrosative species, including hydrogen peroxide (H2O2), aldrithiol-2 (AT-2), glutathione disulfide (GSSG), hypochlorous acid (HOCl), S-nitrosoglutathione (GSNO), peroxynitrite (ONOO−), potassium polysulfide (K2Sx), spermine NONOate (SperNO), and diethyl amino NONOate (DeaNO) at different molar ratios. Fluorescence excitation spectra of the probes were recorded in the excitation wavelength range between 350 and 500 nm and for a total of 60 min. Analysis and interpretation of the data is presented in an associated article.Elsevier2018-05-25T11:22:15Z2018-05-25T11:22:15Z2017-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMÜLLER, A. et al. Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro. Data in Brief, [S.l.], v. 11, p. 617-627, Apr. 2017.http://repositorio.ufla.br/jspui/handle/1/29313Data in Briefreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessMüller, AlexandraSchneider, Jannis F.Degrossoli, AdrianaLupilova, NataliyaDick, Tobias P.Leichert, Lars I.eng2018-05-25T11:23:36Zoai:localhost:1/29313Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2018-05-25T11:23:36Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro |
title |
Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro |
spellingShingle |
Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro Müller, Alexandra Genetically encoded redox probes Polysulfides Hypochlorous acid (HOCl) Peroxynitrite Glutathione Hydrogen peroxide (H2O2) Nitricoxide |
title_short |
Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro |
title_full |
Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro |
title_fullStr |
Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro |
title_full_unstemmed |
Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro |
title_sort |
Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro |
author |
Müller, Alexandra |
author_facet |
Müller, Alexandra Schneider, Jannis F. Degrossoli, Adriana Lupilova, Nataliya Dick, Tobias P. Leichert, Lars I. |
author_role |
author |
author2 |
Schneider, Jannis F. Degrossoli, Adriana Lupilova, Nataliya Dick, Tobias P. Leichert, Lars I. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Müller, Alexandra Schneider, Jannis F. Degrossoli, Adriana Lupilova, Nataliya Dick, Tobias P. Leichert, Lars I. |
dc.subject.por.fl_str_mv |
Genetically encoded redox probes Polysulfides Hypochlorous acid (HOCl) Peroxynitrite Glutathione Hydrogen peroxide (H2O2) Nitricoxide |
topic |
Genetically encoded redox probes Polysulfides Hypochlorous acid (HOCl) Peroxynitrite Glutathione Hydrogen peroxide (H2O2) Nitricoxide |
description |
This article contains representative fluorescence excitation spectra of roGFP2-based probes used for ratiometric analysis of redox changes as presented in the article "Systematic in vitro assessment of responses of roGFP2-based probes to physiologically relevant oxidant species". The recombinant probes roGFP2, roGFP2-Orp1, and Grx1-roGFP2 were exposed to various oxidative and nitrosative species, including hydrogen peroxide (H2O2), aldrithiol-2 (AT-2), glutathione disulfide (GSSG), hypochlorous acid (HOCl), S-nitrosoglutathione (GSNO), peroxynitrite (ONOO−), potassium polysulfide (K2Sx), spermine NONOate (SperNO), and diethyl amino NONOate (DeaNO) at different molar ratios. Fluorescence excitation spectra of the probes were recorded in the excitation wavelength range between 350 and 500 nm and for a total of 60 min. Analysis and interpretation of the data is presented in an associated article. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-04 2018-05-25T11:22:15Z 2018-05-25T11:22:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
MÜLLER, A. et al. Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro. Data in Brief, [S.l.], v. 11, p. 617-627, Apr. 2017. http://repositorio.ufla.br/jspui/handle/1/29313 |
identifier_str_mv |
MÜLLER, A. et al. Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro. Data in Brief, [S.l.], v. 11, p. 617-627, Apr. 2017. |
url |
http://repositorio.ufla.br/jspui/handle/1/29313 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
Data in Brief reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1784549956198072320 |