Reação de clones de batata tolerantes ao calor à pinta preta (Alternaria spp.), podridão mole (Pectobacterium carotovorum), PVY e PVX
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/12996 |
Resumo: | The potato crop (Solanum tuberosum L.) in Brazil is affected by several biotic and abiotic stresses that limit the productive potential of cultivars used by the producers. Among these factors tolerance to high temperatures deserves to be highlighted, since most cultivars used in the country were improved in temperate regions. In addition to high temperatures another limiting factor is the increased inoculum pressure that occur in Brazil when compared to temperate countries. Among the diseases that affect the potato crop the most important are early blight (Alternaria grandis), soft rot (Pectobacterium carotovorum) and PVY and PVX viruses. This work aimed to evaluate the resistance of clones previously selected for tolerance to high temperatures, to the diseases: early blight, soft rot, PVY and PVX. In addition, clones were evaluated in the field for their agronomic performance. A total of 57 clones of the UFLA potato breeding program were used. Early blight resistance was evaluated in vitro by inoculating mycelial discs onto plantlets obtained from tuber buds. Resistance to soft rot was evaluated by inoculating bacterial suspension in the concentration of 10 7 UFC’s in holes of 6 mm in diameter and 1.5 cm deep made directly into the tubers. Resistance to PVY and PVX viruses were evaluated using molecular markers for the Ryadg and Rx1 alleles, respectively. Genotypes resistant or susceptible to each disease were used as controls. The experimental clones were evaluated in two growing seasons in the field for agronomic traits, in the winter of 2015 and in the dry season of 2016, in a randomized complete block design. More productive and responsive clones than the cultivars were found in the field experiments, demonstrating the greater tolerance to heat stress and responsiveness to mild temperatures. We identified clones that showed good agronomic performance and presented resistance to one or more diseases, and the most outstanding were GRO 01-24, GMR 15-38, GMR 03-54, GMR 11-60. We suggest their use in future breeding programs to increase heat tolerance and resistance levels to the diseases evaluated. |
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Reação de clones de batata tolerantes ao calor à pinta preta (Alternaria spp.), podridão mole (Pectobacterium carotovorum), PVY e PVXReaction of potato clones tolerant to heat stress to early blight (Alternaria spp.), soft rot (Pectobacterium carotovorum), PVY and PVXSolanum tuberosum L.Batata - Melhoramento genéticoBatata - Tolerância ao calorBatata - Resistência a doençasMelhoramento VegetalThe potato crop (Solanum tuberosum L.) in Brazil is affected by several biotic and abiotic stresses that limit the productive potential of cultivars used by the producers. Among these factors tolerance to high temperatures deserves to be highlighted, since most cultivars used in the country were improved in temperate regions. In addition to high temperatures another limiting factor is the increased inoculum pressure that occur in Brazil when compared to temperate countries. Among the diseases that affect the potato crop the most important are early blight (Alternaria grandis), soft rot (Pectobacterium carotovorum) and PVY and PVX viruses. This work aimed to evaluate the resistance of clones previously selected for tolerance to high temperatures, to the diseases: early blight, soft rot, PVY and PVX. In addition, clones were evaluated in the field for their agronomic performance. A total of 57 clones of the UFLA potato breeding program were used. Early blight resistance was evaluated in vitro by inoculating mycelial discs onto plantlets obtained from tuber buds. Resistance to soft rot was evaluated by inoculating bacterial suspension in the concentration of 10 7 UFC’s in holes of 6 mm in diameter and 1.5 cm deep made directly into the tubers. Resistance to PVY and PVX viruses were evaluated using molecular markers for the Ryadg and Rx1 alleles, respectively. Genotypes resistant or susceptible to each disease were used as controls. The experimental clones were evaluated in two growing seasons in the field for agronomic traits, in the winter of 2015 and in the dry season of 2016, in a randomized complete block design. More productive and responsive clones than the cultivars were found in the field experiments, demonstrating the greater tolerance to heat stress and responsiveness to mild temperatures. We identified clones that showed good agronomic performance and presented resistance to one or more diseases, and the most outstanding were GRO 01-24, GMR 15-38, GMR 03-54, GMR 11-60. We suggest their use in future breeding programs to increase heat tolerance and resistance levels to the diseases evaluated.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)A cultura da batata (Solanum tuberosum L.) no Brasil é acometida por diversos fatores bióticos e abióticos que limitam o potencial produtivo das cultivares utilizadas pelos produtores. Entre esses fatores a tolerância às altas temperaturas merece destaque, pois a maioria das cultivares utilizadas no país foram melhoradas em países de clima temperado. Além das altas temperaturas outro fator limitante é a maior pressão de patógenos que ocorrem no país, quando comparado aos países temperados. Entre as doenças que acometem a cultura da batata pode-se destacar a pinta preta (Alternaria grandis), a podridão mole (Pectobacterium carotovorum) e as viroses PVY e PVX. Este trabalho objetivou avaliar a resistência de clones previamente selecionados sob condições de altas temperaturas, às doenças: pinta preta, podridão mole, PVY e PVX. Além disso, os clones foram avaliados em campo quanto ao seu desempenho agronômico. Foram utilizados 57 clones do programa de melhoramento de batata da UFLA. A resistência à pinta preta foi avaliada in vitro inoculando discos de micélio em plântulas obtidas por meio das brotações dos tubérculos. A resistência a podridão mole foi avaliada por meio da inoculação de suspensão de bactérias, na concentração de 10 7 UFC’s em orifícios de 6 mm de diâmetro e 1,5 cm de profundidade feitos diretamente nos tubérculos. A resistência às viroses PVY e PVX foi analisada utilizando marcadores moleculares, para a detecção da presença dos alelos Ryadg e Rx1, respectivamente. Como testemunhas foram utilizados genótipos sabidamente resistentes ou suscetíveis para cada doença. Os clones experimentais foram avaliados em duas safras no campo para avaliação dos caracteres agronômicos, na safra de inverno de 2015 e na safra da seca de 2016, em delineamento de blocos completos casualizados. Foram encontrados clones mais produtivos e responsivos que as cultivares, nos experimentos de campo, demonstrando a maior tolerância ao calor e responsividade a temperaturas amenas. Foi possível identificar clones que apresentaram bom desempenho agronômico e resistência à uma ou mais doenças merecendo destaque os clones GRO 01-24, GMR 15-38, GMR 03-54, GMR 11-60. Sugere-se a utilização destes clones como genitores em programas de melhoramento objetivando aumentar a tolerância ao estresse de calor bem como dos níveis de resistência às doenças avaliadas.Universidade Federal de LavrasPrograma de Pós-Graduação em Genética e Melhoramento de PlantasUFLAbrasilDepartamento de BiologiaPinto, César Augusto Brasil PereiraRibeiro, Silvia Regina Rodrigues de PaulaRibeiro, Guilherme Henrique Martins RodriguesSouza, Ricardo Magela deChagas, Rafael Ravaneli2017-05-18T17:23:35Z2017-05-18T17:23:35Z2017-05-172017-02-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCHAGAS, R. R. Reação de clones de batata tolerantes ao calor à pinta preta (Alternaria spp.), podridão mole (Pectobacterium carotovorum), PVY e PVX. 2017. 66 p. Dissertação (Mestrado em Genética e Melhoramento de Plantas)-Universidade Federal de Lavras, Lavras, 2017.http://repositorio.ufla.br/jspui/handle/1/12996porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2017-05-18T17:23:35Zoai:localhost:1/12996Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2017-05-18T17:23:35Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Reação de clones de batata tolerantes ao calor à pinta preta (Alternaria spp.), podridão mole (Pectobacterium carotovorum), PVY e PVX Reaction of potato clones tolerant to heat stress to early blight (Alternaria spp.), soft rot (Pectobacterium carotovorum), PVY and PVX |
title |
Reação de clones de batata tolerantes ao calor à pinta preta (Alternaria spp.), podridão mole (Pectobacterium carotovorum), PVY e PVX |
spellingShingle |
Reação de clones de batata tolerantes ao calor à pinta preta (Alternaria spp.), podridão mole (Pectobacterium carotovorum), PVY e PVX Chagas, Rafael Ravaneli Solanum tuberosum L. Batata - Melhoramento genético Batata - Tolerância ao calor Batata - Resistência a doenças Melhoramento Vegetal |
title_short |
Reação de clones de batata tolerantes ao calor à pinta preta (Alternaria spp.), podridão mole (Pectobacterium carotovorum), PVY e PVX |
title_full |
Reação de clones de batata tolerantes ao calor à pinta preta (Alternaria spp.), podridão mole (Pectobacterium carotovorum), PVY e PVX |
title_fullStr |
Reação de clones de batata tolerantes ao calor à pinta preta (Alternaria spp.), podridão mole (Pectobacterium carotovorum), PVY e PVX |
title_full_unstemmed |
Reação de clones de batata tolerantes ao calor à pinta preta (Alternaria spp.), podridão mole (Pectobacterium carotovorum), PVY e PVX |
title_sort |
Reação de clones de batata tolerantes ao calor à pinta preta (Alternaria spp.), podridão mole (Pectobacterium carotovorum), PVY e PVX |
author |
Chagas, Rafael Ravaneli |
author_facet |
Chagas, Rafael Ravaneli |
author_role |
author |
dc.contributor.none.fl_str_mv |
Pinto, César Augusto Brasil Pereira Ribeiro, Silvia Regina Rodrigues de Paula Ribeiro, Guilherme Henrique Martins Rodrigues Souza, Ricardo Magela de |
dc.contributor.author.fl_str_mv |
Chagas, Rafael Ravaneli |
dc.subject.por.fl_str_mv |
Solanum tuberosum L. Batata - Melhoramento genético Batata - Tolerância ao calor Batata - Resistência a doenças Melhoramento Vegetal |
topic |
Solanum tuberosum L. Batata - Melhoramento genético Batata - Tolerância ao calor Batata - Resistência a doenças Melhoramento Vegetal |
description |
The potato crop (Solanum tuberosum L.) in Brazil is affected by several biotic and abiotic stresses that limit the productive potential of cultivars used by the producers. Among these factors tolerance to high temperatures deserves to be highlighted, since most cultivars used in the country were improved in temperate regions. In addition to high temperatures another limiting factor is the increased inoculum pressure that occur in Brazil when compared to temperate countries. Among the diseases that affect the potato crop the most important are early blight (Alternaria grandis), soft rot (Pectobacterium carotovorum) and PVY and PVX viruses. This work aimed to evaluate the resistance of clones previously selected for tolerance to high temperatures, to the diseases: early blight, soft rot, PVY and PVX. In addition, clones were evaluated in the field for their agronomic performance. A total of 57 clones of the UFLA potato breeding program were used. Early blight resistance was evaluated in vitro by inoculating mycelial discs onto plantlets obtained from tuber buds. Resistance to soft rot was evaluated by inoculating bacterial suspension in the concentration of 10 7 UFC’s in holes of 6 mm in diameter and 1.5 cm deep made directly into the tubers. Resistance to PVY and PVX viruses were evaluated using molecular markers for the Ryadg and Rx1 alleles, respectively. Genotypes resistant or susceptible to each disease were used as controls. The experimental clones were evaluated in two growing seasons in the field for agronomic traits, in the winter of 2015 and in the dry season of 2016, in a randomized complete block design. More productive and responsive clones than the cultivars were found in the field experiments, demonstrating the greater tolerance to heat stress and responsiveness to mild temperatures. We identified clones that showed good agronomic performance and presented resistance to one or more diseases, and the most outstanding were GRO 01-24, GMR 15-38, GMR 03-54, GMR 11-60. We suggest their use in future breeding programs to increase heat tolerance and resistance levels to the diseases evaluated. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-18T17:23:35Z 2017-05-18T17:23:35Z 2017-05-17 2017-02-24 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
CHAGAS, R. R. Reação de clones de batata tolerantes ao calor à pinta preta (Alternaria spp.), podridão mole (Pectobacterium carotovorum), PVY e PVX. 2017. 66 p. Dissertação (Mestrado em Genética e Melhoramento de Plantas)-Universidade Federal de Lavras, Lavras, 2017. http://repositorio.ufla.br/jspui/handle/1/12996 |
identifier_str_mv |
CHAGAS, R. R. Reação de clones de batata tolerantes ao calor à pinta preta (Alternaria spp.), podridão mole (Pectobacterium carotovorum), PVY e PVX. 2017. 66 p. Dissertação (Mestrado em Genética e Melhoramento de Plantas)-Universidade Federal de Lavras, Lavras, 2017. |
url |
http://repositorio.ufla.br/jspui/handle/1/12996 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Genética e Melhoramento de Plantas UFLA brasil Departamento de Biologia |
publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Genética e Melhoramento de Plantas UFLA brasil Departamento de Biologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1807835068515745792 |