Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/38401 |
Resumo: | The objective in this work was to improve the existing methodology for the detection of Sclerotinia sclerotiorum in dry bean and soybean seeds through the use of water restrictors in place of 2,4-dichlorophenoxyacetic acid, in PDA substrate containing bromophenol blue and antibiotics (Neon-S). Firstly, the effects of the water restrictors mannitol, sodium chloride, and polyethyleneglycol (PEG6000) at different osmotic potentials were evaluated on the mycelial growth of S. sclerotiorum isolates and on the radicle protrusion of seeds of the species studied. The use of water restrictors with osmotic potentials of up to -0.5 MPa for PEG and -1.0 MPa for mannitol and sodium chloride did not affect the mycelial growth of the isolates tested, and proved to be satisfactory to inhibit the radicle protrusion of the seeds tested. In the second stage of this work, alternating light and continuous darkness were tested in the detection of fungus in the seeds of both species by the modified Neon method. The methodology composed by solid medium PDA, bromophenol blue (100 ppm), chloramphenicol (50 ppm) and water restrictor mannitol (-1.0 MPa) at a temperature of 20 °C with incubation in darkness, proved to be effective and reliable in the detection of that pathogen in routine analysis laboratory. |
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Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seedsEstabelecimento de um método semi-seletivo para detecção de Sclerotinia sclerotiorum em sementes de feijão e sojaSeed pathologySeed health testsWhite moldPhaseolus vulgarisGlycine maxPatologia de sementesMofo brancoThe objective in this work was to improve the existing methodology for the detection of Sclerotinia sclerotiorum in dry bean and soybean seeds through the use of water restrictors in place of 2,4-dichlorophenoxyacetic acid, in PDA substrate containing bromophenol blue and antibiotics (Neon-S). Firstly, the effects of the water restrictors mannitol, sodium chloride, and polyethyleneglycol (PEG6000) at different osmotic potentials were evaluated on the mycelial growth of S. sclerotiorum isolates and on the radicle protrusion of seeds of the species studied. The use of water restrictors with osmotic potentials of up to -0.5 MPa for PEG and -1.0 MPa for mannitol and sodium chloride did not affect the mycelial growth of the isolates tested, and proved to be satisfactory to inhibit the radicle protrusion of the seeds tested. In the second stage of this work, alternating light and continuous darkness were tested in the detection of fungus in the seeds of both species by the modified Neon method. The methodology composed by solid medium PDA, bromophenol blue (100 ppm), chloramphenicol (50 ppm) and water restrictor mannitol (-1.0 MPa) at a temperature of 20 °C with incubation in darkness, proved to be effective and reliable in the detection of that pathogen in routine analysis laboratory.O objetivo neste trabalho foi definir uma metodologia de detecção de Sclerotinia sclerotiorum em sementes de feijão e soja por meio da utilização de restritores hídricos, em substituição ao uso de ácido 2,4-diclorofenoxiacético, em substrato agar sólido contendo azul de bromofenol e antibióticos (Neon-S). Primeiramente, foram avaliados os efeitos dos restritores hídricos manitol, cloreto de sódio e polietilenoglicol, em diferentes potenciais osmóticos, no crescimento micelial de isolados de Sclerotinia sclerotiorum e na protrusão das radículas das sementes em estudo. O emprego de restritores hídricos com potenciais osmóticos acima de -0.5 MPa para PEG e de -1.0 MPa para manitol e cloreto de sódio não afetou o crescimento micelial dos isolados testados e proporcionou inibição satisfatória da protrusão radicular das sementes em estudo. Na segunda etapa deste trabalho, os regimes alternância de luz e escuro contínuo foram testados na detecção do referido fungo nas sementes em estudo. A metodologia de detecção semi seletiva de S. sclerotiorum com substrato BDA, azul de bromofenol (100ppm), cloranfenicol (50 ppm) e o restritor hídrico manitol (-1.0 MPa), sob temperatura de 20 °C, incubação na ausência de luz, revelou-se mais eficaz e seguro para a detecção do referido patógeno em análises de sanidade de rotina.Associação Brasileira de Tecnologia de Sementes (ABRATES)2020-01-06T12:25:50Z2020-01-06T12:25:50Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfKAWASAKI, V. H.; MACHADO, J. da C. Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds. Journal of Seed Science, Londrina, v. 35, n. 4, p. 435-442, 2013.http://repositorio.ufla.br/jspui/handle/1/38401Journal of Seed Sciencereponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessKawasaki, Vivian HikariMachado, José da Cruzeng2023-05-26T19:56:21Zoai:localhost:1/38401Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T19:56:21Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds Estabelecimento de um método semi-seletivo para detecção de Sclerotinia sclerotiorum em sementes de feijão e soja |
title |
Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds |
spellingShingle |
Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds Kawasaki, Vivian Hikari Seed pathology Seed health tests White mold Phaseolus vulgaris Glycine max Patologia de sementes Mofo branco |
title_short |
Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds |
title_full |
Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds |
title_fullStr |
Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds |
title_full_unstemmed |
Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds |
title_sort |
Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds |
author |
Kawasaki, Vivian Hikari |
author_facet |
Kawasaki, Vivian Hikari Machado, José da Cruz |
author_role |
author |
author2 |
Machado, José da Cruz |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Kawasaki, Vivian Hikari Machado, José da Cruz |
dc.subject.por.fl_str_mv |
Seed pathology Seed health tests White mold Phaseolus vulgaris Glycine max Patologia de sementes Mofo branco |
topic |
Seed pathology Seed health tests White mold Phaseolus vulgaris Glycine max Patologia de sementes Mofo branco |
description |
The objective in this work was to improve the existing methodology for the detection of Sclerotinia sclerotiorum in dry bean and soybean seeds through the use of water restrictors in place of 2,4-dichlorophenoxyacetic acid, in PDA substrate containing bromophenol blue and antibiotics (Neon-S). Firstly, the effects of the water restrictors mannitol, sodium chloride, and polyethyleneglycol (PEG6000) at different osmotic potentials were evaluated on the mycelial growth of S. sclerotiorum isolates and on the radicle protrusion of seeds of the species studied. The use of water restrictors with osmotic potentials of up to -0.5 MPa for PEG and -1.0 MPa for mannitol and sodium chloride did not affect the mycelial growth of the isolates tested, and proved to be satisfactory to inhibit the radicle protrusion of the seeds tested. In the second stage of this work, alternating light and continuous darkness were tested in the detection of fungus in the seeds of both species by the modified Neon method. The methodology composed by solid medium PDA, bromophenol blue (100 ppm), chloramphenicol (50 ppm) and water restrictor mannitol (-1.0 MPa) at a temperature of 20 °C with incubation in darkness, proved to be effective and reliable in the detection of that pathogen in routine analysis laboratory. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2020-01-06T12:25:50Z 2020-01-06T12:25:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
KAWASAKI, V. H.; MACHADO, J. da C. Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds. Journal of Seed Science, Londrina, v. 35, n. 4, p. 435-442, 2013. http://repositorio.ufla.br/jspui/handle/1/38401 |
identifier_str_mv |
KAWASAKI, V. H.; MACHADO, J. da C. Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds. Journal of Seed Science, Londrina, v. 35, n. 4, p. 435-442, 2013. |
url |
http://repositorio.ufla.br/jspui/handle/1/38401 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Tecnologia de Sementes (ABRATES) |
publisher.none.fl_str_mv |
Associação Brasileira de Tecnologia de Sementes (ABRATES) |
dc.source.none.fl_str_mv |
Journal of Seed Science reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835199672680448 |