Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds

Detalhes bibliográficos
Autor(a) principal: Kawasaki, Vivian Hikari
Data de Publicação: 2013
Outros Autores: Machado, José da Cruz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/38401
Resumo: The objective in this work was to improve the existing methodology for the detection of Sclerotinia sclerotiorum in dry bean and soybean seeds through the use of water restrictors in place of 2,4-dichlorophenoxyacetic acid, in PDA substrate containing bromophenol blue and antibiotics (Neon-S). Firstly, the effects of the water restrictors mannitol, sodium chloride, and polyethyleneglycol (PEG6000) at different osmotic potentials were evaluated on the mycelial growth of S. sclerotiorum isolates and on the radicle protrusion of seeds of the species studied. The use of water restrictors with osmotic potentials of up to -0.5 MPa for PEG and -1.0 MPa for mannitol and sodium chloride did not affect the mycelial growth of the isolates tested, and proved to be satisfactory to inhibit the radicle protrusion of the seeds tested. In the second stage of this work, alternating light and continuous darkness were tested in the detection of fungus in the seeds of both species by the modified Neon method. The methodology composed by solid medium PDA, bromophenol blue (100 ppm), chloramphenicol (50 ppm) and water restrictor mannitol (-1.0 MPa) at a temperature of 20 °C with incubation in darkness, proved to be effective and reliable in the detection of that pathogen in routine analysis laboratory.
id UFLA_30b388c774d4824b20c3d41916206eb5
oai_identifier_str oai:localhost:1/38401
network_acronym_str UFLA
network_name_str Repositório Institucional da UFLA
repository_id_str
spelling Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seedsEstabelecimento de um método semi-seletivo para detecção de Sclerotinia sclerotiorum em sementes de feijão e sojaSeed pathologySeed health testsWhite moldPhaseolus vulgarisGlycine maxPatologia de sementesMofo brancoThe objective in this work was to improve the existing methodology for the detection of Sclerotinia sclerotiorum in dry bean and soybean seeds through the use of water restrictors in place of 2,4-dichlorophenoxyacetic acid, in PDA substrate containing bromophenol blue and antibiotics (Neon-S). Firstly, the effects of the water restrictors mannitol, sodium chloride, and polyethyleneglycol (PEG6000) at different osmotic potentials were evaluated on the mycelial growth of S. sclerotiorum isolates and on the radicle protrusion of seeds of the species studied. The use of water restrictors with osmotic potentials of up to -0.5 MPa for PEG and -1.0 MPa for mannitol and sodium chloride did not affect the mycelial growth of the isolates tested, and proved to be satisfactory to inhibit the radicle protrusion of the seeds tested. In the second stage of this work, alternating light and continuous darkness were tested in the detection of fungus in the seeds of both species by the modified Neon method. The methodology composed by solid medium PDA, bromophenol blue (100 ppm), chloramphenicol (50 ppm) and water restrictor mannitol (-1.0 MPa) at a temperature of 20 °C with incubation in darkness, proved to be effective and reliable in the detection of that pathogen in routine analysis laboratory.O objetivo neste trabalho foi definir uma metodologia de detecção de Sclerotinia sclerotiorum em sementes de feijão e soja por meio da utilização de restritores hídricos, em substituição ao uso de ácido 2,4-diclorofenoxiacético, em substrato agar sólido contendo azul de bromofenol e antibióticos (Neon-S). Primeiramente, foram avaliados os efeitos dos restritores hídricos manitol, cloreto de sódio e polietilenoglicol, em diferentes potenciais osmóticos, no crescimento micelial de isolados de Sclerotinia sclerotiorum e na protrusão das radículas das sementes em estudo. O emprego de restritores hídricos com potenciais osmóticos acima de -0.5 MPa para PEG e de -1.0 MPa para manitol e cloreto de sódio não afetou o crescimento micelial dos isolados testados e proporcionou inibição satisfatória da protrusão radicular das sementes em estudo. Na segunda etapa deste trabalho, os regimes alternância de luz e escuro contínuo foram testados na detecção do referido fungo nas sementes em estudo. A metodologia de detecção semi seletiva de S. sclerotiorum com substrato BDA, azul de bromofenol (100ppm), cloranfenicol (50 ppm) e o restritor hídrico manitol (-1.0 MPa), sob temperatura de 20 °C, incubação na ausência de luz, revelou-se mais eficaz e seguro para a detecção do referido patógeno em análises de sanidade de rotina.Associação Brasileira de Tecnologia de Sementes (ABRATES)2020-01-06T12:25:50Z2020-01-06T12:25:50Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfKAWASAKI, V. H.; MACHADO, J. da C. Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds. Journal of Seed Science, Londrina, v. 35, n. 4, p. 435-442, 2013.http://repositorio.ufla.br/jspui/handle/1/38401Journal of Seed Sciencereponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessKawasaki, Vivian HikariMachado, José da Cruzeng2023-05-26T19:56:21Zoai:localhost:1/38401Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T19:56:21Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds
Estabelecimento de um método semi-seletivo para detecção de Sclerotinia sclerotiorum em sementes de feijão e soja
title Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds
spellingShingle Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds
Kawasaki, Vivian Hikari
Seed pathology
Seed health tests
White mold
Phaseolus vulgaris
Glycine max
Patologia de sementes
Mofo branco
title_short Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds
title_full Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds
title_fullStr Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds
title_full_unstemmed Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds
title_sort Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds
author Kawasaki, Vivian Hikari
author_facet Kawasaki, Vivian Hikari
Machado, José da Cruz
author_role author
author2 Machado, José da Cruz
author2_role author
dc.contributor.author.fl_str_mv Kawasaki, Vivian Hikari
Machado, José da Cruz
dc.subject.por.fl_str_mv Seed pathology
Seed health tests
White mold
Phaseolus vulgaris
Glycine max
Patologia de sementes
Mofo branco
topic Seed pathology
Seed health tests
White mold
Phaseolus vulgaris
Glycine max
Patologia de sementes
Mofo branco
description The objective in this work was to improve the existing methodology for the detection of Sclerotinia sclerotiorum in dry bean and soybean seeds through the use of water restrictors in place of 2,4-dichlorophenoxyacetic acid, in PDA substrate containing bromophenol blue and antibiotics (Neon-S). Firstly, the effects of the water restrictors mannitol, sodium chloride, and polyethyleneglycol (PEG6000) at different osmotic potentials were evaluated on the mycelial growth of S. sclerotiorum isolates and on the radicle protrusion of seeds of the species studied. The use of water restrictors with osmotic potentials of up to -0.5 MPa for PEG and -1.0 MPa for mannitol and sodium chloride did not affect the mycelial growth of the isolates tested, and proved to be satisfactory to inhibit the radicle protrusion of the seeds tested. In the second stage of this work, alternating light and continuous darkness were tested in the detection of fungus in the seeds of both species by the modified Neon method. The methodology composed by solid medium PDA, bromophenol blue (100 ppm), chloramphenicol (50 ppm) and water restrictor mannitol (-1.0 MPa) at a temperature of 20 °C with incubation in darkness, proved to be effective and reliable in the detection of that pathogen in routine analysis laboratory.
publishDate 2013
dc.date.none.fl_str_mv 2013
2020-01-06T12:25:50Z
2020-01-06T12:25:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv KAWASAKI, V. H.; MACHADO, J. da C. Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds. Journal of Seed Science, Londrina, v. 35, n. 4, p. 435-442, 2013.
http://repositorio.ufla.br/jspui/handle/1/38401
identifier_str_mv KAWASAKI, V. H.; MACHADO, J. da C. Establishment of a semi-selective method for the detection of sclerotinia sclerotiorum in dry bean and soybean seeds. Journal of Seed Science, Londrina, v. 35, n. 4, p. 435-442, 2013.
url http://repositorio.ufla.br/jspui/handle/1/38401
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Tecnologia de Sementes (ABRATES)
publisher.none.fl_str_mv Associação Brasileira de Tecnologia de Sementes (ABRATES)
dc.source.none.fl_str_mv Journal of Seed Science
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1807835199672680448