Crescimento e aspectos sintomatológicos na aclimatização de Ipê-roxo

• Autor(a) principal: Martins, João Paulo Rodrigues
• Data de Publicação: 2015
• Outros Autores: Santos, Breno Régis, Barbosa, Sandro, Máximo, Wesley Pires Flausino, Beijo, Luiz Alberto, Paiva, Renato
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UFLA
• Texto Completo: http://repositorio.ufla.br/jspui/handle/1/14841
• topic: Handroanthus impetiginosus; Pré-aclimatização; Cultura de tecidos; Vedação; Ipê-roxo; Pre-acclimatization; Tissue culture; Seal

This work evaluated the influence of seal type and substrate type on pre-acclimatization, and symptomatological aspects relating to acclimatization of ipê-roxo. For the pre-acclimatization, ipê-roxo plants obtained through an in vitro zygotic embryo culture were aseptically inoculated in test tubes with different types of seal (cotton plug, plastic cap + parafilm and plastic lid) and different substrates (agar, vermiculite and Plantmax®) supplemented with a WPM culture medium with 1 gL-1 of activated charcoal, 10 mgL-1 of citric acid and 30 gL-1 of sucrose added. For the acclimatization, in vitro ipê-roxo plants were transplanted into 56-cm3 plastic tubes containing vermiculite and Plantmax® at a 2:1 ratio which had been previously autoclaved at 121oC and 1 atm for 20 minutes. Once transplanted, the plants were irrigated with different concentrations of the MS medium (25%, 50%, 75%, 100% and 150%), using distilled water as control. After 30 days, the effects of seal type and substrate type on pre-acclimatization were examined. Cotton can be recommended where Plantmax® or vermiculite is used as substrate. In the acclimatization it was noted that where plants were irrigated with distilled water and concentrations < 50% they showed symptoms of nutritional deficiency. Similar symptoms were observed when using MS medium at a concentration equal to or greater than 100%. The 75% MS concentration was found to be the most effective at maintaining the nutritional vigor of ipê-roxo seedlings in the process of acclimatization.