Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines

Detalhes bibliográficos
Autor(a) principal: Marques, Thaís Lima
Data de Publicação: 2020
Outros Autores: Von Pinho, Renzo Garcia, Von Pinho, Édila Vilela de Resende, Paniago, Bruno da Costa, Freitas, Natália Chagas, Santos, Heloisa Oliveira dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/39914
Resumo: The objective of this study was to evaluate gene expression related to water deficit tolerance in maize lines. For this, lines previously classified as tolerant (91-T and 32-T) and non-tolerant (24-NT and 57-NT) to water deficit were used. The seeds of the four lines were evaluated for emergence and emergence speed index, and the seedlings were evaluated for root and shoot length under two conditions of water availability (70 and 10% substrate water retention capacity). In transcript analysis, the expression of several genes associated with water deficit tolerance, ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A, was evaluated by qRT-PCR for the 91-T and 57-NT lines. It can be concluded that soil water deficiency did not reduce root development. However, the shoot length was significantly lower under dry conditions. Through transcript analysis, the genes ZmDBP3 and ZmAN13 were identified as potential markers for the early selection of maize lines tolerant to water deficit.
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spelling Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize linesAbiotic stressZea maysqRT-PCRWater deficitMilhoAnálise fisiológicaExpressão gênicaEstresse abióticoDéficit hídricoThe objective of this study was to evaluate gene expression related to water deficit tolerance in maize lines. For this, lines previously classified as tolerant (91-T and 32-T) and non-tolerant (24-NT and 57-NT) to water deficit were used. The seeds of the four lines were evaluated for emergence and emergence speed index, and the seedlings were evaluated for root and shoot length under two conditions of water availability (70 and 10% substrate water retention capacity). In transcript analysis, the expression of several genes associated with water deficit tolerance, ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A, was evaluated by qRT-PCR for the 91-T and 57-NT lines. It can be concluded that soil water deficiency did not reduce root development. However, the shoot length was significantly lower under dry conditions. Through transcript analysis, the genes ZmDBP3 and ZmAN13 were identified as potential markers for the early selection of maize lines tolerant to water deficit.Universidade Estadual de Maringá2020-04-08T18:21:01Z2020-04-08T18:21:01Z2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMARQUES, T. L. et al. Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines. Acta Scientiarum. Agronomy, Maringá, v. 42, 2020. Paginação irregular.http://repositorio.ufla.br/jspui/handle/1/39914Acta Scientiarum. Agronomyreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessMarques, Thaís LimaVon Pinho, Renzo GarciaVon Pinho, Édila Vilela de ResendePaniago, Bruno da CostaFreitas, Natália ChagasSantos, Heloisa Oliveira doseng2023-05-26T18:43:54Zoai:localhost:1/39914Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:43:54Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines
title Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines
spellingShingle Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines
Marques, Thaís Lima
Abiotic stress
Zea mays
qRT-PCR
Water deficit
Milho
Análise fisiológica
Expressão gênica
Estresse abiótico
Déficit hídrico
title_short Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines
title_full Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines
title_fullStr Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines
title_full_unstemmed Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines
title_sort Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines
author Marques, Thaís Lima
author_facet Marques, Thaís Lima
Von Pinho, Renzo Garcia
Von Pinho, Édila Vilela de Resende
Paniago, Bruno da Costa
Freitas, Natália Chagas
Santos, Heloisa Oliveira dos
author_role author
author2 Von Pinho, Renzo Garcia
Von Pinho, Édila Vilela de Resende
Paniago, Bruno da Costa
Freitas, Natália Chagas
Santos, Heloisa Oliveira dos
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Marques, Thaís Lima
Von Pinho, Renzo Garcia
Von Pinho, Édila Vilela de Resende
Paniago, Bruno da Costa
Freitas, Natália Chagas
Santos, Heloisa Oliveira dos
dc.subject.por.fl_str_mv Abiotic stress
Zea mays
qRT-PCR
Water deficit
Milho
Análise fisiológica
Expressão gênica
Estresse abiótico
Déficit hídrico
topic Abiotic stress
Zea mays
qRT-PCR
Water deficit
Milho
Análise fisiológica
Expressão gênica
Estresse abiótico
Déficit hídrico
description The objective of this study was to evaluate gene expression related to water deficit tolerance in maize lines. For this, lines previously classified as tolerant (91-T and 32-T) and non-tolerant (24-NT and 57-NT) to water deficit were used. The seeds of the four lines were evaluated for emergence and emergence speed index, and the seedlings were evaluated for root and shoot length under two conditions of water availability (70 and 10% substrate water retention capacity). In transcript analysis, the expression of several genes associated with water deficit tolerance, ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A, was evaluated by qRT-PCR for the 91-T and 57-NT lines. It can be concluded that soil water deficiency did not reduce root development. However, the shoot length was significantly lower under dry conditions. Through transcript analysis, the genes ZmDBP3 and ZmAN13 were identified as potential markers for the early selection of maize lines tolerant to water deficit.
publishDate 2020
dc.date.none.fl_str_mv 2020-04-08T18:21:01Z
2020-04-08T18:21:01Z
2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv MARQUES, T. L. et al. Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines. Acta Scientiarum. Agronomy, Maringá, v. 42, 2020. Paginação irregular.
http://repositorio.ufla.br/jspui/handle/1/39914
identifier_str_mv MARQUES, T. L. et al. Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines. Acta Scientiarum. Agronomy, Maringá, v. 42, 2020. Paginação irregular.
url http://repositorio.ufla.br/jspui/handle/1/39914
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual de Maringá
publisher.none.fl_str_mv Universidade Estadual de Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Agronomy
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1807835208593965056

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