Inhibition of proteases and phospholipases A2 from Bothrops atrox and Crotalus durissus terrificus snake venoms by ascorbic acid, vitamin E, and B-complex vitamins

Detalhes bibliográficos
Autor(a) principal: Oliveira, Carlos H. M.
Data de Publicação: 2016
Outros Autores: Simão, Anderson A., Trento, Marcus V. C., César, Pedro H. S., Marcussi, Silvana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/32522
Resumo: The enzyme inhibition by natural and/ or low-cost compounds may represent a valuable adjunct to traditional serotherapy performed in cases of snakebite, mainly with a view to mitigate the local effects of envenoming. The objective of this study was to evaluate possible interactions between vitamins and enzymes that comprise Bothrops atrox and Crotalus durissus terrificus venoms, in vitro. Proteolysis inhibition assays (substrates: azocasein, collagen, gelatin and fibrinogen), hemolysis, coagulation, hemagglutination were carried out using different proportions of vitamins in face of to inhibit minimum effective dose of each venom. The vitamins were responsible for reducing 100% of breaking azocasein by C.d.t. venom, thrombolysis induced by B. atrox and fibrinogenolysis induced by both venoms. It is suggested the presence of interactions between vitamin and the active site of enzymes, for example the interactions between hydrophobic regions present in the enzymes and vitamin E, as well as the inhibitions exercised by antioxidant mechanism.
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spelling Inhibition of proteases and phospholipases A2 from Bothrops atrox and Crotalus durissus terrificus snake venoms by ascorbic acid, vitamin E, and B-complex vitaminsAntiophidian propertiesAntioxidantsBlood cellsHemostatic processesNatural inhibitorsPropriedades antiofídicasAntioxidantesCélulas sanguíneasProcessos hemostáticosInibidores naturaisThe enzyme inhibition by natural and/ or low-cost compounds may represent a valuable adjunct to traditional serotherapy performed in cases of snakebite, mainly with a view to mitigate the local effects of envenoming. The objective of this study was to evaluate possible interactions between vitamins and enzymes that comprise Bothrops atrox and Crotalus durissus terrificus venoms, in vitro. Proteolysis inhibition assays (substrates: azocasein, collagen, gelatin and fibrinogen), hemolysis, coagulation, hemagglutination were carried out using different proportions of vitamins in face of to inhibit minimum effective dose of each venom. The vitamins were responsible for reducing 100% of breaking azocasein by C.d.t. venom, thrombolysis induced by B. atrox and fibrinogenolysis induced by both venoms. It is suggested the presence of interactions between vitamin and the active site of enzymes, for example the interactions between hydrophobic regions present in the enzymes and vitamin E, as well as the inhibitions exercised by antioxidant mechanism.A inibição enzimática por compostos naturais e/ou de baixo custo pode representar um valioso adjuvante ao tratamento soroterápico tradicional realizado em casos de ofidismo, com vistas a amenizar principalmente os efeitos locais do envenenamento. Objetivou-se avaliar in vitro possíveis interações entre vitaminas e enzimas presentes nas peçonhas de Bothrops atrox e Crotalus durissus terrificus. Ensaios de inibição de proteólise (substratos: azocaseína, colágeno, gelatina e fibrinogênio), hemólise, coagulação, hemaglutinação, foram realizados utilizando diferentes proporções de vitaminas em face de inibir à dose mínima efetiva de cada peçonha. As vitaminas foram responsáveis por reduzir 100% a quebra da azocaseína por peçonha de C.d.t., a trombólise induzida por B. atrox e a fibrinogenólise induzida por ambas as peçonhas. Sugere-se a presença de interações de vitaminas com o sítio ativo das enzimas, como por exemplo, interações entre regiões hidrofóbicas presentes nas enzimas e vitamina E, assim como, inibição de algumas atividades por mecanismo de ação antioxidante.Academia Brasileira de Ciências2019-01-25T10:35:33Z2019-01-25T10:35:33Z2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfOLIVEIRA, C. H. M. et al. Inhibition of proteases and phospholipases A2 from Bothrops atrox and Crotalus durissus terrificus snake venoms by ascorbic acid, vitamin E, and B-complex vitamins. Anais da Academia Brasileira de Ciências, v. 88, n. 3, p. 2005-2016, 2016. Suplemento 3.http://repositorio.ufla.br/jspui/handle/1/32522Anais da Academia Brasileira de Ciênciasreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessOliveira, Carlos H. M.Simão, Anderson A.Trento, Marcus V. C.César, Pedro H. S.Marcussi, Silvanaeng2019-01-25T10:35:34Zoai:localhost:1/32522Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-01-25T10:35:34Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Inhibition of proteases and phospholipases A2 from Bothrops atrox and Crotalus durissus terrificus snake venoms by ascorbic acid, vitamin E, and B-complex vitamins
title Inhibition of proteases and phospholipases A2 from Bothrops atrox and Crotalus durissus terrificus snake venoms by ascorbic acid, vitamin E, and B-complex vitamins
spellingShingle Inhibition of proteases and phospholipases A2 from Bothrops atrox and Crotalus durissus terrificus snake venoms by ascorbic acid, vitamin E, and B-complex vitamins
Oliveira, Carlos H. M.
Antiophidian properties
Antioxidants
Blood cells
Hemostatic processes
Natural inhibitors
Propriedades antiofídicas
Antioxidantes
Células sanguíneas
Processos hemostáticos
Inibidores naturais
title_short Inhibition of proteases and phospholipases A2 from Bothrops atrox and Crotalus durissus terrificus snake venoms by ascorbic acid, vitamin E, and B-complex vitamins
title_full Inhibition of proteases and phospholipases A2 from Bothrops atrox and Crotalus durissus terrificus snake venoms by ascorbic acid, vitamin E, and B-complex vitamins
title_fullStr Inhibition of proteases and phospholipases A2 from Bothrops atrox and Crotalus durissus terrificus snake venoms by ascorbic acid, vitamin E, and B-complex vitamins
title_full_unstemmed Inhibition of proteases and phospholipases A2 from Bothrops atrox and Crotalus durissus terrificus snake venoms by ascorbic acid, vitamin E, and B-complex vitamins
title_sort Inhibition of proteases and phospholipases A2 from Bothrops atrox and Crotalus durissus terrificus snake venoms by ascorbic acid, vitamin E, and B-complex vitamins
author Oliveira, Carlos H. M.
author_facet Oliveira, Carlos H. M.
Simão, Anderson A.
Trento, Marcus V. C.
César, Pedro H. S.
Marcussi, Silvana
author_role author
author2 Simão, Anderson A.
Trento, Marcus V. C.
César, Pedro H. S.
Marcussi, Silvana
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Carlos H. M.
Simão, Anderson A.
Trento, Marcus V. C.
César, Pedro H. S.
Marcussi, Silvana
dc.subject.por.fl_str_mv Antiophidian properties
Antioxidants
Blood cells
Hemostatic processes
Natural inhibitors
Propriedades antiofídicas
Antioxidantes
Células sanguíneas
Processos hemostáticos
Inibidores naturais
topic Antiophidian properties
Antioxidants
Blood cells
Hemostatic processes
Natural inhibitors
Propriedades antiofídicas
Antioxidantes
Células sanguíneas
Processos hemostáticos
Inibidores naturais
description The enzyme inhibition by natural and/ or low-cost compounds may represent a valuable adjunct to traditional serotherapy performed in cases of snakebite, mainly with a view to mitigate the local effects of envenoming. The objective of this study was to evaluate possible interactions between vitamins and enzymes that comprise Bothrops atrox and Crotalus durissus terrificus venoms, in vitro. Proteolysis inhibition assays (substrates: azocasein, collagen, gelatin and fibrinogen), hemolysis, coagulation, hemagglutination were carried out using different proportions of vitamins in face of to inhibit minimum effective dose of each venom. The vitamins were responsible for reducing 100% of breaking azocasein by C.d.t. venom, thrombolysis induced by B. atrox and fibrinogenolysis induced by both venoms. It is suggested the presence of interactions between vitamin and the active site of enzymes, for example the interactions between hydrophobic regions present in the enzymes and vitamin E, as well as the inhibitions exercised by antioxidant mechanism.
publishDate 2016
dc.date.none.fl_str_mv 2016
2019-01-25T10:35:33Z
2019-01-25T10:35:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv OLIVEIRA, C. H. M. et al. Inhibition of proteases and phospholipases A2 from Bothrops atrox and Crotalus durissus terrificus snake venoms by ascorbic acid, vitamin E, and B-complex vitamins. Anais da Academia Brasileira de Ciências, v. 88, n. 3, p. 2005-2016, 2016. Suplemento 3.
http://repositorio.ufla.br/jspui/handle/1/32522
identifier_str_mv OLIVEIRA, C. H. M. et al. Inhibition of proteases and phospholipases A2 from Bothrops atrox and Crotalus durissus terrificus snake venoms by ascorbic acid, vitamin E, and B-complex vitamins. Anais da Academia Brasileira de Ciências, v. 88, n. 3, p. 2005-2016, 2016. Suplemento 3.
url http://repositorio.ufla.br/jspui/handle/1/32522
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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