Atividades antioxidante e fungitóxica do óleo essencial de capim-limão e do citral

Detalhes bibliográficos
Autor(a) principal: Guimarães, Luiz Gustavo de Lima
Data de Publicação: 2011
Outros Autores: Cardoso, Maria das Graças, Sousa, Paulo Estevão de, Andrade, Juliana de, Vieira, Sara Silveira
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/41893
Resumo: Given the importance of essential oils, this study evaluated the antioxidant activity and fungitoxic effects of lemongrass essential oil and its main constituent citral on mycelial inhibition of phytopathogens Fusarium oxysporum cubense, Colletotrichum gloeosporioides, Bipolaris sp. and Alternaria alternata. The antioxidant activity was evaluated by employing the DPPH free radical reduction method and oxidation tests of the system β-carotene/linoleic acid. For each method, a completely randomized design in a 4 x 5 factorial arrangement was used with four compounds (essential oil, citral, thymol and α-tocopherol) and five concentrations (5; 10; 25; 50 and 100 Mg L-1), with three replicates. The tests to determine fungitoxic activities were performed by applying the in vitro fumigation test on mycelial inhibition of studied phytopathogens. The treatments were arranged in a completely randomized design with three replicates and a factorial arrangement. The essential oil and citral showed little antioxidant activity by the methodology that measures the reduction of the stable radical DPPH; however, by the methodology that employs the emulsified β-carotene/linoleic acid system, these compounds showed significant antioxidant activities. At fungitoxic activities, the essential oil and citral showed greater activity against the phytopathogen Alternaria alternata. When compared to the values of IC50, the lowest values presented by the essential (75.83 µg mL-1) and citral (58.24 µg mL-1) oils were found for Bipolaris sp. Citral caused the greatest mycelial inhibition on all phytopathogens.
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spelling Atividades antioxidante e fungitóxica do óleo essencial de capim-limão e do citralAntioxidant and fungitoxic activities of the lemongrass essential oil and citralCymbopogon citratusFitopatógenosAtividade biológicaMetabólitos secundáriosPhytopathogensBiological activitySecondary metabolitesGiven the importance of essential oils, this study evaluated the antioxidant activity and fungitoxic effects of lemongrass essential oil and its main constituent citral on mycelial inhibition of phytopathogens Fusarium oxysporum cubense, Colletotrichum gloeosporioides, Bipolaris sp. and Alternaria alternata. The antioxidant activity was evaluated by employing the DPPH free radical reduction method and oxidation tests of the system β-carotene/linoleic acid. For each method, a completely randomized design in a 4 x 5 factorial arrangement was used with four compounds (essential oil, citral, thymol and α-tocopherol) and five concentrations (5; 10; 25; 50 and 100 Mg L-1), with three replicates. The tests to determine fungitoxic activities were performed by applying the in vitro fumigation test on mycelial inhibition of studied phytopathogens. The treatments were arranged in a completely randomized design with three replicates and a factorial arrangement. The essential oil and citral showed little antioxidant activity by the methodology that measures the reduction of the stable radical DPPH; however, by the methodology that employs the emulsified β-carotene/linoleic acid system, these compounds showed significant antioxidant activities. At fungitoxic activities, the essential oil and citral showed greater activity against the phytopathogen Alternaria alternata. When compared to the values of IC50, the lowest values presented by the essential (75.83 µg mL-1) and citral (58.24 µg mL-1) oils were found for Bipolaris sp. Citral caused the greatest mycelial inhibition on all phytopathogens.Tendo em vista a importância dos óleos essenciais, objetivou-se, com este trabalho, avaliar a atividade antioxidante e os efeitos fungitóxicos do óleo essencial de capim-limão e do seu constituinte majoritário citral sobre a inibição micelial dos fitopatógenos Fusarium oxysporum cubense, Colletotrichum gloeosporioides, Bipolaris sp. e Alternaria alternata. A atividade antioxidante foi realizada, empregando os métodos de redução do radical livre DPPH e o ensaio de oxidação do sistema b-caroteno/ácido linoléico. Para cada metodologia, utilizou-se o delineamento inteiramente casualizado em esquema fatorial de 4 x 5, sendo quatro compostos (óleo essencial, citral, timol e α-tocoferol) e cinco concentrações (5; 10; 25; 50 e 100 µg L-1), com três repetições Os ensaios para verificação das atividades fungitóxicas foram realizados aplicando o teste de fumigação in vitro sobre a inibição micelial dos fungos fitopatogênicos estudados. Os tratamentos foram dispostos de forma inteiramente casualizada, com três repetições e em esquema fatorial com arranjo variável entre os fitopatógenos. O óleo essencial e o citral apresentaram pequena atividade antioxidante perante a metodologia que avalia a redução do radical estável DPPH, no entanto, perante a metodologia que emprega o sistema emulsificado β-caroteno/ácido linoléico estes compostos apresentaram atividades antioxidantes significativas. Nas atividades fungitóxicas, o óleo essencial e o citral apresentaram uma maior atividade sobre o fitopatógeno Alternaria alternata. Em relação aos valores de IC50 os menores valores apresentados pelo óleo essencial (75,83 µg mL-1) e pelo citral (58,24 µg mL-1) foram sobre o Bipolaris sp. O citral causou as maiores inibições miceliais, para todos os fitopatógenos.Universidade Federal do Ceará (UFC)2020-07-13T10:52:22Z2020-07-13T10:52:22Z2011-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfGUIMARÃES, L. G. de L. et al. Atividades antioxidante e fungitóxica do óleo essencial de capim-limão e do citral. Revista Ciência Agronômica, Fortaleza, v. 42, n. 2, p. 464-472, abr./jun. 2011. DOI: 10.1590/S1806-66902011000200028.http://repositorio.ufla.br/jspui/handle/1/41893Revista Ciência Agronômicareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessGuimarães, Luiz Gustavo de LimaCardoso, Maria das GraçasSousa, Paulo Estevão deAndrade, Juliana deVieira, Sara Silveirapor2020-07-13T10:52:22Zoai:localhost:1/41893Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-07-13T10:52:22Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Atividades antioxidante e fungitóxica do óleo essencial de capim-limão e do citral
Antioxidant and fungitoxic activities of the lemongrass essential oil and citral
title Atividades antioxidante e fungitóxica do óleo essencial de capim-limão e do citral
spellingShingle Atividades antioxidante e fungitóxica do óleo essencial de capim-limão e do citral
Guimarães, Luiz Gustavo de Lima
Cymbopogon citratus
Fitopatógenos
Atividade biológica
Metabólitos secundários
Phytopathogens
Biological activity
Secondary metabolites
title_short Atividades antioxidante e fungitóxica do óleo essencial de capim-limão e do citral
title_full Atividades antioxidante e fungitóxica do óleo essencial de capim-limão e do citral
title_fullStr Atividades antioxidante e fungitóxica do óleo essencial de capim-limão e do citral
title_full_unstemmed Atividades antioxidante e fungitóxica do óleo essencial de capim-limão e do citral
title_sort Atividades antioxidante e fungitóxica do óleo essencial de capim-limão e do citral
author Guimarães, Luiz Gustavo de Lima
author_facet Guimarães, Luiz Gustavo de Lima
Cardoso, Maria das Graças
Sousa, Paulo Estevão de
Andrade, Juliana de
Vieira, Sara Silveira
author_role author
author2 Cardoso, Maria das Graças
Sousa, Paulo Estevão de
Andrade, Juliana de
Vieira, Sara Silveira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Guimarães, Luiz Gustavo de Lima
Cardoso, Maria das Graças
Sousa, Paulo Estevão de
Andrade, Juliana de
Vieira, Sara Silveira
dc.subject.por.fl_str_mv Cymbopogon citratus
Fitopatógenos
Atividade biológica
Metabólitos secundários
Phytopathogens
Biological activity
Secondary metabolites
topic Cymbopogon citratus
Fitopatógenos
Atividade biológica
Metabólitos secundários
Phytopathogens
Biological activity
Secondary metabolites
description Given the importance of essential oils, this study evaluated the antioxidant activity and fungitoxic effects of lemongrass essential oil and its main constituent citral on mycelial inhibition of phytopathogens Fusarium oxysporum cubense, Colletotrichum gloeosporioides, Bipolaris sp. and Alternaria alternata. The antioxidant activity was evaluated by employing the DPPH free radical reduction method and oxidation tests of the system β-carotene/linoleic acid. For each method, a completely randomized design in a 4 x 5 factorial arrangement was used with four compounds (essential oil, citral, thymol and α-tocopherol) and five concentrations (5; 10; 25; 50 and 100 Mg L-1), with three replicates. The tests to determine fungitoxic activities were performed by applying the in vitro fumigation test on mycelial inhibition of studied phytopathogens. The treatments were arranged in a completely randomized design with three replicates and a factorial arrangement. The essential oil and citral showed little antioxidant activity by the methodology that measures the reduction of the stable radical DPPH; however, by the methodology that employs the emulsified β-carotene/linoleic acid system, these compounds showed significant antioxidant activities. At fungitoxic activities, the essential oil and citral showed greater activity against the phytopathogen Alternaria alternata. When compared to the values of IC50, the lowest values presented by the essential (75.83 µg mL-1) and citral (58.24 µg mL-1) oils were found for Bipolaris sp. Citral caused the greatest mycelial inhibition on all phytopathogens.
publishDate 2011
dc.date.none.fl_str_mv 2011-06
2020-07-13T10:52:22Z
2020-07-13T10:52:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv GUIMARÃES, L. G. de L. et al. Atividades antioxidante e fungitóxica do óleo essencial de capim-limão e do citral. Revista Ciência Agronômica, Fortaleza, v. 42, n. 2, p. 464-472, abr./jun. 2011. DOI: 10.1590/S1806-66902011000200028.
http://repositorio.ufla.br/jspui/handle/1/41893
identifier_str_mv GUIMARÃES, L. G. de L. et al. Atividades antioxidante e fungitóxica do óleo essencial de capim-limão e do citral. Revista Ciência Agronômica, Fortaleza, v. 42, n. 2, p. 464-472, abr./jun. 2011. DOI: 10.1590/S1806-66902011000200028.
url http://repositorio.ufla.br/jspui/handle/1/41893
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do Ceará (UFC)
publisher.none.fl_str_mv Universidade Federal do Ceará (UFC)
dc.source.none.fl_str_mv Revista Ciência Agronômica
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
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instname_str Universidade Federal de Lavras (UFLA)
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reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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