Methods of asepsis for in vitro establishment and germination of Eucalyptus grandis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/37293 |
Resumo: | Technical and scientific studies have been conducted for the cultivation of Eucalyptus, with special attention to large-scale clonal propagation. However, in some studies are necessary to obtain aseptic material from seeds, and as far as we are aware efficient protocols of asepsis for this purpose are still not available. Therefore, this study aimed to define a protocol for sterilization of seeds of Eucalyptus grandis for in vitro establishment and germination. Four methods of sterilization were tested using different application sequences of the disinfectants, as benomyl fungicide, hydroalcoholic solution (70% v/v) and sodium hypochlorite (NaOCl). The experiment was conducted in completely randomized design with five repetitions. The percentage of establishment and contamination were evaluated at the end of the experiment. Washing with sterile water and 70% alcohol for 30 s, 2.5% sodium hypochlorite for 20 min, 1% benomyl for 20 min and three washes with sterile water was the best method found, accounting to 87.5% of establishment. |
id |
UFLA_3dc94bec81da8b1be3cafbb74949e04a |
---|---|
oai_identifier_str |
oai:localhost:1/37293 |
network_acronym_str |
UFLA |
network_name_str |
Repositório Institucional da UFLA |
repository_id_str |
|
spelling |
Methods of asepsis for in vitro establishment and germination of Eucalyptus grandisMétodos de assepsia para estabelecimento in vitro e germinação de Eucalyptus grandisDisinfestation of seedsWater-alcohol solutionSodium hypochloriteIn vitro cultureTechnical and scientific studies have been conducted for the cultivation of Eucalyptus, with special attention to large-scale clonal propagation. However, in some studies are necessary to obtain aseptic material from seeds, and as far as we are aware efficient protocols of asepsis for this purpose are still not available. Therefore, this study aimed to define a protocol for sterilization of seeds of Eucalyptus grandis for in vitro establishment and germination. Four methods of sterilization were tested using different application sequences of the disinfectants, as benomyl fungicide, hydroalcoholic solution (70% v/v) and sodium hypochlorite (NaOCl). The experiment was conducted in completely randomized design with five repetitions. The percentage of establishment and contamination were evaluated at the end of the experiment. Washing with sterile water and 70% alcohol for 30 s, 2.5% sodium hypochlorite for 20 min, 1% benomyl for 20 min and three washes with sterile water was the best method found, accounting to 87.5% of establishment.2019-10-18T16:12:33Z2019-10-18T16:12:33Z2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfALCÂNTARA, B. K. et al. Methods of asepsis for in vitro establishment and germination of Eucalyptus grandis. Journal of Biotechnology and Biodiversity, [S.l.], v. 2, n. 3, 2011.http://repositorio.ufla.br/jspui/handle/1/37293Journal of Biotechnology and Biodiversityreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessAlcântara, Berenice KussumotoBrondani, Gilvano EblingGonçalves, Antônio NatalAlmeida, Marcílio deAzevedo, Ricardo Antuneseng2019-10-18T16:12:57Zoai:localhost:1/37293Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-10-18T16:12:57Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Methods of asepsis for in vitro establishment and germination of Eucalyptus grandis Métodos de assepsia para estabelecimento in vitro e germinação de Eucalyptus grandis |
title |
Methods of asepsis for in vitro establishment and germination of Eucalyptus grandis |
spellingShingle |
Methods of asepsis for in vitro establishment and germination of Eucalyptus grandis Alcântara, Berenice Kussumoto Disinfestation of seeds Water-alcohol solution Sodium hypochlorite In vitro culture |
title_short |
Methods of asepsis for in vitro establishment and germination of Eucalyptus grandis |
title_full |
Methods of asepsis for in vitro establishment and germination of Eucalyptus grandis |
title_fullStr |
Methods of asepsis for in vitro establishment and germination of Eucalyptus grandis |
title_full_unstemmed |
Methods of asepsis for in vitro establishment and germination of Eucalyptus grandis |
title_sort |
Methods of asepsis for in vitro establishment and germination of Eucalyptus grandis |
author |
Alcântara, Berenice Kussumoto |
author_facet |
Alcântara, Berenice Kussumoto Brondani, Gilvano Ebling Gonçalves, Antônio Natal Almeida, Marcílio de Azevedo, Ricardo Antunes |
author_role |
author |
author2 |
Brondani, Gilvano Ebling Gonçalves, Antônio Natal Almeida, Marcílio de Azevedo, Ricardo Antunes |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Alcântara, Berenice Kussumoto Brondani, Gilvano Ebling Gonçalves, Antônio Natal Almeida, Marcílio de Azevedo, Ricardo Antunes |
dc.subject.por.fl_str_mv |
Disinfestation of seeds Water-alcohol solution Sodium hypochlorite In vitro culture |
topic |
Disinfestation of seeds Water-alcohol solution Sodium hypochlorite In vitro culture |
description |
Technical and scientific studies have been conducted for the cultivation of Eucalyptus, with special attention to large-scale clonal propagation. However, in some studies are necessary to obtain aseptic material from seeds, and as far as we are aware efficient protocols of asepsis for this purpose are still not available. Therefore, this study aimed to define a protocol for sterilization of seeds of Eucalyptus grandis for in vitro establishment and germination. Four methods of sterilization were tested using different application sequences of the disinfectants, as benomyl fungicide, hydroalcoholic solution (70% v/v) and sodium hypochlorite (NaOCl). The experiment was conducted in completely randomized design with five repetitions. The percentage of establishment and contamination were evaluated at the end of the experiment. Washing with sterile water and 70% alcohol for 30 s, 2.5% sodium hypochlorite for 20 min, 1% benomyl for 20 min and three washes with sterile water was the best method found, accounting to 87.5% of establishment. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 2019-10-18T16:12:33Z 2019-10-18T16:12:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
ALCÂNTARA, B. K. et al. Methods of asepsis for in vitro establishment and germination of Eucalyptus grandis. Journal of Biotechnology and Biodiversity, [S.l.], v. 2, n. 3, 2011. http://repositorio.ufla.br/jspui/handle/1/37293 |
identifier_str_mv |
ALCÂNTARA, B. K. et al. Methods of asepsis for in vitro establishment and germination of Eucalyptus grandis. Journal of Biotechnology and Biodiversity, [S.l.], v. 2, n. 3, 2011. |
url |
http://repositorio.ufla.br/jspui/handle/1/37293 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Journal of Biotechnology and Biodiversity reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835150590935040 |