Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000500016 http://repositorio.ufla.br/jspui/handle/1/5956 |
Resumo: | The study was carried out in order to evaluate the effect of temperature and in vitor stigma receptivity on regeneration of orange cultivar (Valência, Pêra and Natal) pollen. Two experiments were carried out, in the first the ideal temperature of germination was assessed. Pollen was obtained from flowers at the balloon stage and inoculated in culture medium with 10 g L-1 agar, 200 mg L-1 boric acid, 100 g L-1 sucrose, 800 mg L-1 calcium nitrate, pH adjusted to 6,5 and incubated in a BOD chamber at temperatures of 23, 24, 25, 26 and 27ºC during a 24-hour photoperiod. After 12 hours of incubation, the best temperature for pollen grain germination was 25ºC for all varieties. In a second experiment, in order to test the receptivity of the stigma, flowers were collected at different flowering stages: small bud, balloon and open flower. The stigmas were by immersion exposed to hydrogen peroxide (perioxidase reaction), 3% for 3 minutes. Through the technique of Zeisler (1938), better results were detected at the balloon stage with 80 to 100% receptivity, while for the open flower the receptivity presented maximum indexes. |
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Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperaturesCitrus sinensisPalinologytissue cultureThe study was carried out in order to evaluate the effect of temperature and in vitor stigma receptivity on regeneration of orange cultivar (Valência, Pêra and Natal) pollen. Two experiments were carried out, in the first the ideal temperature of germination was assessed. Pollen was obtained from flowers at the balloon stage and inoculated in culture medium with 10 g L-1 agar, 200 mg L-1 boric acid, 100 g L-1 sucrose, 800 mg L-1 calcium nitrate, pH adjusted to 6,5 and incubated in a BOD chamber at temperatures of 23, 24, 25, 26 and 27ºC during a 24-hour photoperiod. After 12 hours of incubation, the best temperature for pollen grain germination was 25ºC for all varieties. In a second experiment, in order to test the receptivity of the stigma, flowers were collected at different flowering stages: small bud, balloon and open flower. The stigmas were by immersion exposed to hydrogen peroxide (perioxidase reaction), 3% for 3 minutes. Through the technique of Zeisler (1938), better results were detected at the balloon stage with 80 to 100% receptivity, while for the open flower the receptivity presented maximum indexes.Editora da Universidade Federal de Lavras2004-10-012015-04-30T13:33:14Z2015-04-30T13:33:14Z2015-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000500016http://repositorio.ufla.br/jspui/handle/1/5956Ciência e Agrotecnologia v.28 n.5 2004reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAPio,Leila Aparecida SallesRamos,José DarlanPasqual,MoacirSantos,Flavia CarvalhoJunqueira,Keize Pereiraenginfo:eu-repo/semantics/openAccess2015-04-30T13:33:14Zoai:localhost:1/5956Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2015-04-30T13:33:14Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures |
title |
Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures |
spellingShingle |
Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures Pio,Leila Aparecida Salles Citrus sinensis Palinology tissue culture |
title_short |
Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures |
title_full |
Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures |
title_fullStr |
Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures |
title_full_unstemmed |
Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures |
title_sort |
Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures |
author |
Pio,Leila Aparecida Salles |
author_facet |
Pio,Leila Aparecida Salles Ramos,José Darlan Pasqual,Moacir Santos,Flavia Carvalho Junqueira,Keize Pereira |
author_role |
author |
author2 |
Ramos,José Darlan Pasqual,Moacir Santos,Flavia Carvalho Junqueira,Keize Pereira |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Pio,Leila Aparecida Salles Ramos,José Darlan Pasqual,Moacir Santos,Flavia Carvalho Junqueira,Keize Pereira |
dc.subject.por.fl_str_mv |
Citrus sinensis Palinology tissue culture |
topic |
Citrus sinensis Palinology tissue culture |
description |
The study was carried out in order to evaluate the effect of temperature and in vitor stigma receptivity on regeneration of orange cultivar (Valência, Pêra and Natal) pollen. Two experiments were carried out, in the first the ideal temperature of germination was assessed. Pollen was obtained from flowers at the balloon stage and inoculated in culture medium with 10 g L-1 agar, 200 mg L-1 boric acid, 100 g L-1 sucrose, 800 mg L-1 calcium nitrate, pH adjusted to 6,5 and incubated in a BOD chamber at temperatures of 23, 24, 25, 26 and 27ºC during a 24-hour photoperiod. After 12 hours of incubation, the best temperature for pollen grain germination was 25ºC for all varieties. In a second experiment, in order to test the receptivity of the stigma, flowers were collected at different flowering stages: small bud, balloon and open flower. The stigmas were by immersion exposed to hydrogen peroxide (perioxidase reaction), 3% for 3 minutes. Through the technique of Zeisler (1938), better results were detected at the balloon stage with 80 to 100% receptivity, while for the open flower the receptivity presented maximum indexes. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-10-01 2015-04-30T13:33:14Z 2015-04-30T13:33:14Z 2015-04-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000500016 http://repositorio.ufla.br/jspui/handle/1/5956 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000500016 http://repositorio.ufla.br/jspui/handle/1/5956 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
dc.source.none.fl_str_mv |
Ciência e Agrotecnologia v.28 n.5 2004 reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1784549986132819968 |