Transcriptome analyses suggest that changes in fungal endophyte lifestyle could be involved in grapevine bud necrosis
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/42788 |
Resumo: | Bud necrosis (BN) is a common disorder that affects Vitis vinifera L. and reduces its potential yield. To minimize the losses caused by BN, the double pruning management was applied in Brazilian Southeast vineyards. In this management strategy plants are pruned at the winter to promote a vegetative cycle and then, at summer, to promote the reproductive cycle at optimal environmental conditions. To investigate the relationship of BN and the double pruning management RNA-seq libraries were sequenced from healthy and necrotic tissues at four different stages of the year. The comparison of differentially expressed genes in necrotic and non-necrotic tissues showed an enhanced expression of genes related to cell death possibly induced by endophytic microorganisms in the necrotic tissues. The de novo assembly, characterization and quantification of transcripts within the RNA-seq libraries showed that genes from the endophytic fungus Alternaria alternata, responsible for the production of toxic compounds were highly expressed under BN. Here we propose a model in which unfavorable conditions and reduced carbohydrate levels in buds can promote the switch from a biotrophic lifestyle to a necrotrophic lifestyle in the endophytic fungi, which seems to be involved in the development of BN. |
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Transcriptome analyses suggest that changes in fungal endophyte lifestyle could be involved in grapevine bud necrosisGrapevine - DiseasesDouble pruning managementRNA-seq librariesAlternaria alternataVideira - Doenças e pragasVideira - Dupla podaNecrose da gemaMicrorganismos endofíticosBud necrosis (BN) is a common disorder that affects Vitis vinifera L. and reduces its potential yield. To minimize the losses caused by BN, the double pruning management was applied in Brazilian Southeast vineyards. In this management strategy plants are pruned at the winter to promote a vegetative cycle and then, at summer, to promote the reproductive cycle at optimal environmental conditions. To investigate the relationship of BN and the double pruning management RNA-seq libraries were sequenced from healthy and necrotic tissues at four different stages of the year. The comparison of differentially expressed genes in necrotic and non-necrotic tissues showed an enhanced expression of genes related to cell death possibly induced by endophytic microorganisms in the necrotic tissues. The de novo assembly, characterization and quantification of transcripts within the RNA-seq libraries showed that genes from the endophytic fungus Alternaria alternata, responsible for the production of toxic compounds were highly expressed under BN. Here we propose a model in which unfavorable conditions and reduced carbohydrate levels in buds can promote the switch from a biotrophic lifestyle to a necrotrophic lifestyle in the endophytic fungi, which seems to be involved in the development of BN.Springer Nature2020-09-01T17:30:52Z2020-09-01T17:30:52Z2020-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfRIBEIRO, T. H. C. et al. Transcriptome analyses suggest that changes in fungal endophyte lifestyle could be involved in grapevine bud necrosis. Scientific Reports, [S. I.], v. 10, 2020. DOI: https://doi.org/10.1038/s41598-020-66500-0.http://repositorio.ufla.br/jspui/handle/1/42788Scientific Reportsreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessRibeiro, Thales Henrique CherubinoBrum, Christiane Noronha FernandesSouza, Claudia Rita deDias, Frederico Alcantara NovelliAlmeida Junior, Osmar deRegina, Murilo de AlbuquerqueOliveira, Kellen Kauanne Pimenta deReis, Gabriel Lasmar dosOliveira, Larissa MaiaFernandes, Fernanda de PaulaTorregrosa, LaurentSouza, Jorge Teodoro deChalfun Junior, Antonioeng2020-09-01T17:31:08Zoai:localhost:1/42788Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-09-01T17:31:08Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Transcriptome analyses suggest that changes in fungal endophyte lifestyle could be involved in grapevine bud necrosis |
title |
Transcriptome analyses suggest that changes in fungal endophyte lifestyle could be involved in grapevine bud necrosis |
spellingShingle |
Transcriptome analyses suggest that changes in fungal endophyte lifestyle could be involved in grapevine bud necrosis Ribeiro, Thales Henrique Cherubino Grapevine - Diseases Double pruning management RNA-seq libraries Alternaria alternata Videira - Doenças e pragas Videira - Dupla poda Necrose da gema Microrganismos endofíticos |
title_short |
Transcriptome analyses suggest that changes in fungal endophyte lifestyle could be involved in grapevine bud necrosis |
title_full |
Transcriptome analyses suggest that changes in fungal endophyte lifestyle could be involved in grapevine bud necrosis |
title_fullStr |
Transcriptome analyses suggest that changes in fungal endophyte lifestyle could be involved in grapevine bud necrosis |
title_full_unstemmed |
Transcriptome analyses suggest that changes in fungal endophyte lifestyle could be involved in grapevine bud necrosis |
title_sort |
Transcriptome analyses suggest that changes in fungal endophyte lifestyle could be involved in grapevine bud necrosis |
author |
Ribeiro, Thales Henrique Cherubino |
author_facet |
Ribeiro, Thales Henrique Cherubino Brum, Christiane Noronha Fernandes Souza, Claudia Rita de Dias, Frederico Alcantara Novelli Almeida Junior, Osmar de Regina, Murilo de Albuquerque Oliveira, Kellen Kauanne Pimenta de Reis, Gabriel Lasmar dos Oliveira, Larissa Maia Fernandes, Fernanda de Paula Torregrosa, Laurent Souza, Jorge Teodoro de Chalfun Junior, Antonio |
author_role |
author |
author2 |
Brum, Christiane Noronha Fernandes Souza, Claudia Rita de Dias, Frederico Alcantara Novelli Almeida Junior, Osmar de Regina, Murilo de Albuquerque Oliveira, Kellen Kauanne Pimenta de Reis, Gabriel Lasmar dos Oliveira, Larissa Maia Fernandes, Fernanda de Paula Torregrosa, Laurent Souza, Jorge Teodoro de Chalfun Junior, Antonio |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ribeiro, Thales Henrique Cherubino Brum, Christiane Noronha Fernandes Souza, Claudia Rita de Dias, Frederico Alcantara Novelli Almeida Junior, Osmar de Regina, Murilo de Albuquerque Oliveira, Kellen Kauanne Pimenta de Reis, Gabriel Lasmar dos Oliveira, Larissa Maia Fernandes, Fernanda de Paula Torregrosa, Laurent Souza, Jorge Teodoro de Chalfun Junior, Antonio |
dc.subject.por.fl_str_mv |
Grapevine - Diseases Double pruning management RNA-seq libraries Alternaria alternata Videira - Doenças e pragas Videira - Dupla poda Necrose da gema Microrganismos endofíticos |
topic |
Grapevine - Diseases Double pruning management RNA-seq libraries Alternaria alternata Videira - Doenças e pragas Videira - Dupla poda Necrose da gema Microrganismos endofíticos |
description |
Bud necrosis (BN) is a common disorder that affects Vitis vinifera L. and reduces its potential yield. To minimize the losses caused by BN, the double pruning management was applied in Brazilian Southeast vineyards. In this management strategy plants are pruned at the winter to promote a vegetative cycle and then, at summer, to promote the reproductive cycle at optimal environmental conditions. To investigate the relationship of BN and the double pruning management RNA-seq libraries were sequenced from healthy and necrotic tissues at four different stages of the year. The comparison of differentially expressed genes in necrotic and non-necrotic tissues showed an enhanced expression of genes related to cell death possibly induced by endophytic microorganisms in the necrotic tissues. The de novo assembly, characterization and quantification of transcripts within the RNA-seq libraries showed that genes from the endophytic fungus Alternaria alternata, responsible for the production of toxic compounds were highly expressed under BN. Here we propose a model in which unfavorable conditions and reduced carbohydrate levels in buds can promote the switch from a biotrophic lifestyle to a necrotrophic lifestyle in the endophytic fungi, which seems to be involved in the development of BN. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09-01T17:30:52Z 2020-09-01T17:30:52Z 2020-06 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
RIBEIRO, T. H. C. et al. Transcriptome analyses suggest that changes in fungal endophyte lifestyle could be involved in grapevine bud necrosis. Scientific Reports, [S. I.], v. 10, 2020. DOI: https://doi.org/10.1038/s41598-020-66500-0. http://repositorio.ufla.br/jspui/handle/1/42788 |
identifier_str_mv |
RIBEIRO, T. H. C. et al. Transcriptome analyses suggest that changes in fungal endophyte lifestyle could be involved in grapevine bud necrosis. Scientific Reports, [S. I.], v. 10, 2020. DOI: https://doi.org/10.1038/s41598-020-66500-0. |
url |
http://repositorio.ufla.br/jspui/handle/1/42788 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Nature |
publisher.none.fl_str_mv |
Springer Nature |
dc.source.none.fl_str_mv |
Scientific Reports reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835123653017600 |