Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda

Detalhes bibliográficos
Autor(a) principal: Costa, Maria L. M.
Data de Publicação: 2014
Outros Autores: Lana, Ubiraci G. P., Barros, Emerson C., Paiva, Luciano V., Valicente, Fernando H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/41410
Resumo: Bacillus thuringiensis (Bt) endotoxins (Cyt and Cry) have been extensively explored for biological control of fall armyworm, Spodoptera frugiperda, an important pest in major corn-producing countries of America. Five hundred Bt isolates with different toxicities against S. frugiperda were characterized by PCR for the presence of cyt genes (cyt1, cyt2, cyt1Aa, cyt1Ab, cyt2B and cyt2Ba), and the effect of insecticidal proteins Cry1Ba, Cry1Ca, Cry1Da and Cyt on S. frugiperda larvae were evaluated. Six isolates showed the presence of cyt genes, three isolates harbored two gene families (cyt1 and cyt2), and three isolates harbored only one of the cyt gene families (cyt1 and cyt2). It was not possible to correlate the presence/absence of cyt genes with toxicity against S. frugiperda. In this study, cyt genes were present in toxic and nontoxic isolates to this insect pest. Bioassays against S. frugiperda larvae showed that only Cry1Ca protein had toxicity, with 77.08% of mortality. Synergism among Cry and Cry proteins used in this study against S. frugiperda was not observed.
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spelling Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperdaBacillus thuringiensisCyt genesEntomopathogenic bacteriaCyt proteinsCry proteinsBacillus thuringiensis (Bt) endotoxins (Cyt and Cry) have been extensively explored for biological control of fall armyworm, Spodoptera frugiperda, an important pest in major corn-producing countries of America. Five hundred Bt isolates with different toxicities against S. frugiperda were characterized by PCR for the presence of cyt genes (cyt1, cyt2, cyt1Aa, cyt1Ab, cyt2B and cyt2Ba), and the effect of insecticidal proteins Cry1Ba, Cry1Ca, Cry1Da and Cyt on S. frugiperda larvae were evaluated. Six isolates showed the presence of cyt genes, three isolates harbored two gene families (cyt1 and cyt2), and three isolates harbored only one of the cyt gene families (cyt1 and cyt2). It was not possible to correlate the presence/absence of cyt genes with toxicity against S. frugiperda. In this study, cyt genes were present in toxic and nontoxic isolates to this insect pest. Bioassays against S. frugiperda larvae showed that only Cry1Ca protein had toxicity, with 77.08% of mortality. Synergism among Cry and Cry proteins used in this study against S. frugiperda was not observed.Canadian Center of Science and Education (CCSE)2020-06-14T02:13:07Z2020-06-14T02:13:07Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCOSTA, M. L. M. et al. Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda. Journal of Agricultural Science, [S.l.], v. 6, n. 7, p. 1-10, 2014. DOI: 10.5539/jas.v6n7p128.http://repositorio.ufla.br/jspui/handle/1/41410Journal of Agricultural Science (JAS)reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessCosta, Maria L. M.Lana, Ubiraci G. P.Barros, Emerson C.Paiva, Luciano V.Valicente, Fernando H.eng2020-06-14T02:13:07Zoai:localhost:1/41410Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-06-14T02:13:07Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda
title Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda
spellingShingle Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda
Costa, Maria L. M.
Bacillus thuringiensis
Cyt genes
Entomopathogenic bacteria
Cyt proteins
Cry proteins
title_short Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda
title_full Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda
title_fullStr Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda
title_full_unstemmed Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda
title_sort Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda
author Costa, Maria L. M.
author_facet Costa, Maria L. M.
Lana, Ubiraci G. P.
Barros, Emerson C.
Paiva, Luciano V.
Valicente, Fernando H.
author_role author
author2 Lana, Ubiraci G. P.
Barros, Emerson C.
Paiva, Luciano V.
Valicente, Fernando H.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Costa, Maria L. M.
Lana, Ubiraci G. P.
Barros, Emerson C.
Paiva, Luciano V.
Valicente, Fernando H.
dc.subject.por.fl_str_mv Bacillus thuringiensis
Cyt genes
Entomopathogenic bacteria
Cyt proteins
Cry proteins
topic Bacillus thuringiensis
Cyt genes
Entomopathogenic bacteria
Cyt proteins
Cry proteins
description Bacillus thuringiensis (Bt) endotoxins (Cyt and Cry) have been extensively explored for biological control of fall armyworm, Spodoptera frugiperda, an important pest in major corn-producing countries of America. Five hundred Bt isolates with different toxicities against S. frugiperda were characterized by PCR for the presence of cyt genes (cyt1, cyt2, cyt1Aa, cyt1Ab, cyt2B and cyt2Ba), and the effect of insecticidal proteins Cry1Ba, Cry1Ca, Cry1Da and Cyt on S. frugiperda larvae were evaluated. Six isolates showed the presence of cyt genes, three isolates harbored two gene families (cyt1 and cyt2), and three isolates harbored only one of the cyt gene families (cyt1 and cyt2). It was not possible to correlate the presence/absence of cyt genes with toxicity against S. frugiperda. In this study, cyt genes were present in toxic and nontoxic isolates to this insect pest. Bioassays against S. frugiperda larvae showed that only Cry1Ca protein had toxicity, with 77.08% of mortality. Synergism among Cry and Cry proteins used in this study against S. frugiperda was not observed.
publishDate 2014
dc.date.none.fl_str_mv 2014
2020-06-14T02:13:07Z
2020-06-14T02:13:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv COSTA, M. L. M. et al. Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda. Journal of Agricultural Science, [S.l.], v. 6, n. 7, p. 1-10, 2014. DOI: 10.5539/jas.v6n7p128.
http://repositorio.ufla.br/jspui/handle/1/41410
identifier_str_mv COSTA, M. L. M. et al. Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda. Journal of Agricultural Science, [S.l.], v. 6, n. 7, p. 1-10, 2014. DOI: 10.5539/jas.v6n7p128.
url http://repositorio.ufla.br/jspui/handle/1/41410
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Canadian Center of Science and Education (CCSE)
publisher.none.fl_str_mv Canadian Center of Science and Education (CCSE)
dc.source.none.fl_str_mv Journal of Agricultural Science (JAS)
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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