Assessment of the occurrence and richness of arbuscular mycorrhizal fungal spores by direct analysis of field samples and trap culture - a comparative study

Detalhes bibliográficos
Autor(a) principal: Leal, Patrícia L.
Data de Publicação: 2018
Outros Autores: Carvalho, Teotonio Soares de, Siqueira, José Oswaldo, Moreira, Fatima Maria Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/30333
Resumo: In this work, we hypothesized that two spore-based methods, direct analysis of field samples and trap cultures, simultaneously used for assessment of occurrence and species richness of arbuscular mycorrhizal fungi (AMF) may vary in their efficiency according to the environmental conditions and the total AMF species richness of the evaluated ecosystem. The performance of both methods was analyzed based on two datasets: 1) a complete site x species matrix compiled from two studies in different land uses in the Amazon using direct analysis of field samples and trap cultures. 2) Total number of AMF morphotypes detected by both methods in published manuscripts across several ecosystems. From dataset 1, direct analysis of field samples revealed 57 morphotypes, whereas only 21 of these were detected by trap culture. Community variation (beta diversity) analysis revealed that field samples are far more sensitive in detecting shifts in AMF community composition among land uses than trap cultures in the Amazon region, with the combined results of both methods being not better than that obtained only by direct analysis of field samples. Analysis of dataset 2 showed that the relative performance of trap cultures, using direct analysis of field sample as reference, was inversely related to the total observed AMF species richness.
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spelling Assessment of the occurrence and richness of arbuscular mycorrhizal fungal spores by direct analysis of field samples and trap culture - a comparative studyAlpha and beta diversityDiversity assessment methodsGlomeromycotaSpore countsTaxonomyDiversidade alfa e betaMétodos de avaliação de diversidadeContagens de esporosTaxonomiaIn this work, we hypothesized that two spore-based methods, direct analysis of field samples and trap cultures, simultaneously used for assessment of occurrence and species richness of arbuscular mycorrhizal fungi (AMF) may vary in their efficiency according to the environmental conditions and the total AMF species richness of the evaluated ecosystem. The performance of both methods was analyzed based on two datasets: 1) a complete site x species matrix compiled from two studies in different land uses in the Amazon using direct analysis of field samples and trap cultures. 2) Total number of AMF morphotypes detected by both methods in published manuscripts across several ecosystems. From dataset 1, direct analysis of field samples revealed 57 morphotypes, whereas only 21 of these were detected by trap culture. Community variation (beta diversity) analysis revealed that field samples are far more sensitive in detecting shifts in AMF community composition among land uses than trap cultures in the Amazon region, with the combined results of both methods being not better than that obtained only by direct analysis of field samples. Analysis of dataset 2 showed that the relative performance of trap cultures, using direct analysis of field sample as reference, was inversely related to the total observed AMF species richness.Academia Brasileira de Ciências2018-08-31T12:59:37Z2018-08-31T12:59:37Z2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfLEAL, P. L. et al. Assessment of the occurrence and richness of arbuscular mycorrhizal fungal spores by direct analysis of field samples and trap culture - a comparative study. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 90, n. 2, p. 2359-2373, 2018. Suplemento 1.http://repositorio.ufla.br/jspui/handle/1/30333Anais da Academia Brasileira de Ciênciasreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessLeal, Patrícia L.Carvalho, Teotonio Soares deSiqueira, José OswaldoMoreira, Fatima Maria Souzaeng2018-08-31T12:59:37Zoai:localhost:1/30333Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2018-08-31T12:59:37Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Assessment of the occurrence and richness of arbuscular mycorrhizal fungal spores by direct analysis of field samples and trap culture - a comparative study
title Assessment of the occurrence and richness of arbuscular mycorrhizal fungal spores by direct analysis of field samples and trap culture - a comparative study
spellingShingle Assessment of the occurrence and richness of arbuscular mycorrhizal fungal spores by direct analysis of field samples and trap culture - a comparative study
Leal, Patrícia L.
Alpha and beta diversity
Diversity assessment methods
Glomeromycota
Spore counts
Taxonomy
Diversidade alfa e beta
Métodos de avaliação de diversidade
Contagens de esporos
Taxonomia
title_short Assessment of the occurrence and richness of arbuscular mycorrhizal fungal spores by direct analysis of field samples and trap culture - a comparative study
title_full Assessment of the occurrence and richness of arbuscular mycorrhizal fungal spores by direct analysis of field samples and trap culture - a comparative study
title_fullStr Assessment of the occurrence and richness of arbuscular mycorrhizal fungal spores by direct analysis of field samples and trap culture - a comparative study
title_full_unstemmed Assessment of the occurrence and richness of arbuscular mycorrhizal fungal spores by direct analysis of field samples and trap culture - a comparative study
title_sort Assessment of the occurrence and richness of arbuscular mycorrhizal fungal spores by direct analysis of field samples and trap culture - a comparative study
author Leal, Patrícia L.
author_facet Leal, Patrícia L.
Carvalho, Teotonio Soares de
Siqueira, José Oswaldo
Moreira, Fatima Maria Souza
author_role author
author2 Carvalho, Teotonio Soares de
Siqueira, José Oswaldo
Moreira, Fatima Maria Souza
author2_role author
author
author
dc.contributor.author.fl_str_mv Leal, Patrícia L.
Carvalho, Teotonio Soares de
Siqueira, José Oswaldo
Moreira, Fatima Maria Souza
dc.subject.por.fl_str_mv Alpha and beta diversity
Diversity assessment methods
Glomeromycota
Spore counts
Taxonomy
Diversidade alfa e beta
Métodos de avaliação de diversidade
Contagens de esporos
Taxonomia
topic Alpha and beta diversity
Diversity assessment methods
Glomeromycota
Spore counts
Taxonomy
Diversidade alfa e beta
Métodos de avaliação de diversidade
Contagens de esporos
Taxonomia
description In this work, we hypothesized that two spore-based methods, direct analysis of field samples and trap cultures, simultaneously used for assessment of occurrence and species richness of arbuscular mycorrhizal fungi (AMF) may vary in their efficiency according to the environmental conditions and the total AMF species richness of the evaluated ecosystem. The performance of both methods was analyzed based on two datasets: 1) a complete site x species matrix compiled from two studies in different land uses in the Amazon using direct analysis of field samples and trap cultures. 2) Total number of AMF morphotypes detected by both methods in published manuscripts across several ecosystems. From dataset 1, direct analysis of field samples revealed 57 morphotypes, whereas only 21 of these were detected by trap culture. Community variation (beta diversity) analysis revealed that field samples are far more sensitive in detecting shifts in AMF community composition among land uses than trap cultures in the Amazon region, with the combined results of both methods being not better than that obtained only by direct analysis of field samples. Analysis of dataset 2 showed that the relative performance of trap cultures, using direct analysis of field sample as reference, was inversely related to the total observed AMF species richness.
publishDate 2018
dc.date.none.fl_str_mv 2018-08-31T12:59:37Z
2018-08-31T12:59:37Z
2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv LEAL, P. L. et al. Assessment of the occurrence and richness of arbuscular mycorrhizal fungal spores by direct analysis of field samples and trap culture - a comparative study. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 90, n. 2, p. 2359-2373, 2018. Suplemento 1.
http://repositorio.ufla.br/jspui/handle/1/30333
identifier_str_mv LEAL, P. L. et al. Assessment of the occurrence and richness of arbuscular mycorrhizal fungal spores by direct analysis of field samples and trap culture - a comparative study. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 90, n. 2, p. 2359-2373, 2018. Suplemento 1.
url http://repositorio.ufla.br/jspui/handle/1/30333
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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