Chalcone analogues on the control of Meloidogyne incognita and genomic studies of the biocontrol agent Bacillus velezensis

Detalhes bibliográficos
Autor(a) principal: Silva, Fabíola de Jesus
Data de Publicação: 2020
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/41083
Resumo: Root-knot nematodes, Meloidogyne spp., are economically important plant pathogens and distributed worldwide. Among the strategies used to control these pathogens are chemical nematicides. However, the overuse of these toxic molecules in agriculture causes serious environmental problems. Hence the need for sustainable strategies, such as the use of natural compounds and biological agents. Chalcones are intermediate and final products in the flavonoid biosynthesis route. They have a wide spectrum of important biological activities. In this work, the activity of 12 chalcone analogues against the root-knot nematode Meloidogyne incognita was studied. Three of them showed strong nematicidal and nematostatic action against second stage juveniles of M. incognita. Chalcone (1E,4E)-1,5-di(4-nitrophenyl)-2-butylpenta-1,4-dien-3-one (compound 6) showed higher nematicidal activity than the commercial nematicide Carbofuran, and when applied in infested tomato plants, it reduced the number of nematode galls and eggs by 51% and 68%, respectively. In in silico study, this chalcone presumably acts by inhibiting the cytochrome P450 enzyme, which is important in the oxidation of various substances in nematode physiology. Also in this work, we studied the complete genomic sequence of the strain Bacillus velezensis UFLA258, which is a biological control agent of plant pathogens, since its obtaining, followed by assembly and annotation. In addition, using a comparative genomic approach, in silico evaluations were performed with all complete B. velezensis genomes available in the database, plus genomes of nearby species Bacillus amyloliquefaciens and Bacillus siamensis. Thus, the B. velezensis UFLA258 genome consists of a single chromosome of 3.95 Mbp in length, with an average GC content of 46.69%. It contains 3,949 protein coding genes and 27 RNA genes. Analysis based on mean nucleotide identity (ANI), DNA-DNA hybridization (dDDH), and complete sequence phylogeny of the rpoB gene confirmed that 19 strains deposited in the database as B. amyloliquefaciens were indeed B. velezensis. Although these species are phylogenetically close, combined analyzes of various genomic traits, such as the presence of biosynthetic genes encoding secondary metabolites, CRISPr/Cas arrays, ANI, dDDH, and other strain information, including source isolation, have allowed their unambiguous classification like these three species. This genomic analysis extends knowledge about related species, B. velezensis, B. amyloliquefaciens and B. siamensis, with an emphasis on taxonomic status.
id UFLA_4f365ba265abc3a7838a825eb8a97ca8
oai_identifier_str oai:localhost:1/41083
network_acronym_str UFLA
network_name_str Repositório Institucional da UFLA
repository_id_str
spelling Chalcone analogues on the control of Meloidogyne incognita and genomic studies of the biocontrol agent Bacillus velezensisNematoide das galhasChalconasNematicidas químicosPatógenos vegetais - Controle biológicoSequenciamento de genomaRoot-knot nematodesChemical nematicidesPlant pathogens - Biological controlGenome sequencingFitopatologiaRoot-knot nematodes, Meloidogyne spp., are economically important plant pathogens and distributed worldwide. Among the strategies used to control these pathogens are chemical nematicides. However, the overuse of these toxic molecules in agriculture causes serious environmental problems. Hence the need for sustainable strategies, such as the use of natural compounds and biological agents. Chalcones are intermediate and final products in the flavonoid biosynthesis route. They have a wide spectrum of important biological activities. In this work, the activity of 12 chalcone analogues against the root-knot nematode Meloidogyne incognita was studied. Three of them showed strong nematicidal and nematostatic action against second stage juveniles of M. incognita. Chalcone (1E,4E)-1,5-di(4-nitrophenyl)-2-butylpenta-1,4-dien-3-one (compound 6) showed higher nematicidal activity than the commercial nematicide Carbofuran, and when applied in infested tomato plants, it reduced the number of nematode galls and eggs by 51% and 68%, respectively. In in silico study, this chalcone presumably acts by inhibiting the cytochrome P450 enzyme, which is important in the oxidation of various substances in nematode physiology. Also in this work, we studied the complete genomic sequence of the strain Bacillus velezensis UFLA258, which is a biological control agent of plant pathogens, since its obtaining, followed by assembly and annotation. In addition, using a comparative genomic approach, in silico evaluations were performed with all complete B. velezensis genomes available in the database, plus genomes of nearby species Bacillus amyloliquefaciens and Bacillus siamensis. Thus, the B. velezensis UFLA258 genome consists of a single chromosome of 3.95 Mbp in length, with an average GC content of 46.69%. It contains 3,949 protein coding genes and 27 RNA genes. Analysis based on mean nucleotide identity (ANI), DNA-DNA hybridization (dDDH), and complete sequence phylogeny of the rpoB gene confirmed that 19 strains deposited in the database as B. amyloliquefaciens were indeed B. velezensis. Although these species are phylogenetically close, combined analyzes of various genomic traits, such as the presence of biosynthetic genes encoding secondary metabolites, CRISPr/Cas arrays, ANI, dDDH, and other strain information, including source isolation, have allowed their unambiguous classification like these three species. This genomic analysis extends knowledge about related species, B. velezensis, B. amyloliquefaciens and B. siamensis, with an emphasis on taxonomic status.Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Os nematoides das galhas, Meloidogyne spp., são patógenos vegetais economicamente importantes e distribuídos em todo o mundo. Dentre as estratégias utilizadas para o controle desses patógenos estão os nematicidas químicos. No entanto, o uso excessivo dessas moléculas tóxicas na agricultura causam sérios problemas ambientais. Daí a necessidade de estratégias sustentáveis, como por exemplo, o uso de compostos naturais e agentes biológicos. As chalconas são produtos intermediários e finais na rota de biossintese dos flavonoides. Elas possuem um amplo espectro de atividades biológicas importantes. Neste trabalho, estudou-se a atividade de 12 análogos de chalconas contra o nematoide das galhas Meloidogyne incognita. Três deles mostraram forte ação nematicida e nematostática contra juvenis de segundo estádio de M. incognita. A chalcona (1E,4E)-1,5-di(4-nitrofenil)-2-butilpenta-1,4-dien-3-ona (composto 6) apresentou maior atividade nematicida do que o nematicida comercial Carbofuran, e quando aplicada em tomateiros infestados, reduziu o número de galhas e ovos do nematoides em 51% e 68%, respectivamente. Em estudo in silico, esta chalcona atua, presumivelmente, inibindo a enzima citocromo P450, que é importante na oxidação de diversas substâncias na fisiologia do nematoide. Ainda neste trabalho, estudou-se o genoma completo da estirpe Bacillus velezensis UFLA258, que é um agente de controle biológico de patógenos vegetais, desde a sua obtenção, seguida da montagem e anotação. Adicionalmente, usando uma abordagem genômica comparativa, realizaram-se avaliações in silico com todos os genomas completos de B. velezensis disponíveis no banco de dados, mais os genomas completos das espécies próximas Bacillus amyloliquefaciens e Bacillus siamensis. Assim, o genoma de B. velezensis UFLA258 consiste em um único cromossomo de 3,95 Mbp de comprimento, com um teor médio de GC de 46,69%. Contém 3.949 genes codificadores de proteína e 27 genes de RNA. Análises baseadas na identidade média dos nucleotídieos (ANI), hibridização DNA-DNA (dDDH) e filogenia com sequências completas do gene rpoB confirmaram que 19 cepas depositadas no banco de dados como B. amyloliquefaciens eram de fato B. velezensis. Embora essas espécies sejam filogeneticamente próximas, as análises combinadas de várias características genômicas, como presença de genes biossintéticos codificadores de metabólitos secundários, arranjos CRISPr/Cas, ANI, dDDH, e outras informações sobre as cepas, incluindo fonte de isolamento, permitiram sua classificação inequívoca como estas três espécies. Esta análise genômica amplia o conhecimento sobre as espécies aparentadas, B. velezensis, B. amyloliquefaciens e B. siamensis, com ênfase no status taxonômico.Universidade Federal de LavrasPrograma de Pós-Graduação em Agronomia/FitopatologiaUFLAbrasilDepartamento de FitopatologiaCampos, Vicente PauloBarros, Aline FerreiraBarros, Aline FerreiraMagalhães, Valter CruzSalgado, Sonia Maria de LimaOliveira, Denilson Ferreira deSilva, Fabíola de Jesus2020-05-20T13:01:08Z2020-05-20T13:01:08Z2020-05-202020-03-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfSILVA, F. de J. Chalcone analogues on the control of Meloidogyne incognita and genomic studies of the biocontrol agent Bacillus velezensis. 2020. 86 p. Tese (Doutorado em Agronomia/Fitopatologia)-Universidade Federal de Lavras, Lavras, 2020.http://repositorio.ufla.br/jspui/handle/1/41083porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2023-05-11T18:24:19Zoai:localhost:1/41083Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-11T18:24:19Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Chalcone analogues on the control of Meloidogyne incognita and genomic studies of the biocontrol agent Bacillus velezensis
title Chalcone analogues on the control of Meloidogyne incognita and genomic studies of the biocontrol agent Bacillus velezensis
spellingShingle Chalcone analogues on the control of Meloidogyne incognita and genomic studies of the biocontrol agent Bacillus velezensis
Silva, Fabíola de Jesus
Nematoide das galhas
Chalconas
Nematicidas químicos
Patógenos vegetais - Controle biológico
Sequenciamento de genoma
Root-knot nematodes
Chemical nematicides
Plant pathogens - Biological control
Genome sequencing
Fitopatologia
title_short Chalcone analogues on the control of Meloidogyne incognita and genomic studies of the biocontrol agent Bacillus velezensis
title_full Chalcone analogues on the control of Meloidogyne incognita and genomic studies of the biocontrol agent Bacillus velezensis
title_fullStr Chalcone analogues on the control of Meloidogyne incognita and genomic studies of the biocontrol agent Bacillus velezensis
title_full_unstemmed Chalcone analogues on the control of Meloidogyne incognita and genomic studies of the biocontrol agent Bacillus velezensis
title_sort Chalcone analogues on the control of Meloidogyne incognita and genomic studies of the biocontrol agent Bacillus velezensis
author Silva, Fabíola de Jesus
author_facet Silva, Fabíola de Jesus
author_role author
dc.contributor.none.fl_str_mv Campos, Vicente Paulo
Barros, Aline Ferreira
Barros, Aline Ferreira
Magalhães, Valter Cruz
Salgado, Sonia Maria de Lima
Oliveira, Denilson Ferreira de
dc.contributor.author.fl_str_mv Silva, Fabíola de Jesus
dc.subject.por.fl_str_mv Nematoide das galhas
Chalconas
Nematicidas químicos
Patógenos vegetais - Controle biológico
Sequenciamento de genoma
Root-knot nematodes
Chemical nematicides
Plant pathogens - Biological control
Genome sequencing
Fitopatologia
topic Nematoide das galhas
Chalconas
Nematicidas químicos
Patógenos vegetais - Controle biológico
Sequenciamento de genoma
Root-knot nematodes
Chemical nematicides
Plant pathogens - Biological control
Genome sequencing
Fitopatologia
description Root-knot nematodes, Meloidogyne spp., are economically important plant pathogens and distributed worldwide. Among the strategies used to control these pathogens are chemical nematicides. However, the overuse of these toxic molecules in agriculture causes serious environmental problems. Hence the need for sustainable strategies, such as the use of natural compounds and biological agents. Chalcones are intermediate and final products in the flavonoid biosynthesis route. They have a wide spectrum of important biological activities. In this work, the activity of 12 chalcone analogues against the root-knot nematode Meloidogyne incognita was studied. Three of them showed strong nematicidal and nematostatic action against second stage juveniles of M. incognita. Chalcone (1E,4E)-1,5-di(4-nitrophenyl)-2-butylpenta-1,4-dien-3-one (compound 6) showed higher nematicidal activity than the commercial nematicide Carbofuran, and when applied in infested tomato plants, it reduced the number of nematode galls and eggs by 51% and 68%, respectively. In in silico study, this chalcone presumably acts by inhibiting the cytochrome P450 enzyme, which is important in the oxidation of various substances in nematode physiology. Also in this work, we studied the complete genomic sequence of the strain Bacillus velezensis UFLA258, which is a biological control agent of plant pathogens, since its obtaining, followed by assembly and annotation. In addition, using a comparative genomic approach, in silico evaluations were performed with all complete B. velezensis genomes available in the database, plus genomes of nearby species Bacillus amyloliquefaciens and Bacillus siamensis. Thus, the B. velezensis UFLA258 genome consists of a single chromosome of 3.95 Mbp in length, with an average GC content of 46.69%. It contains 3,949 protein coding genes and 27 RNA genes. Analysis based on mean nucleotide identity (ANI), DNA-DNA hybridization (dDDH), and complete sequence phylogeny of the rpoB gene confirmed that 19 strains deposited in the database as B. amyloliquefaciens were indeed B. velezensis. Although these species are phylogenetically close, combined analyzes of various genomic traits, such as the presence of biosynthetic genes encoding secondary metabolites, CRISPr/Cas arrays, ANI, dDDH, and other strain information, including source isolation, have allowed their unambiguous classification like these three species. This genomic analysis extends knowledge about related species, B. velezensis, B. amyloliquefaciens and B. siamensis, with an emphasis on taxonomic status.
publishDate 2020
dc.date.none.fl_str_mv 2020-05-20T13:01:08Z
2020-05-20T13:01:08Z
2020-05-20
2020-03-05
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv SILVA, F. de J. Chalcone analogues on the control of Meloidogyne incognita and genomic studies of the biocontrol agent Bacillus velezensis. 2020. 86 p. Tese (Doutorado em Agronomia/Fitopatologia)-Universidade Federal de Lavras, Lavras, 2020.
http://repositorio.ufla.br/jspui/handle/1/41083
identifier_str_mv SILVA, F. de J. Chalcone analogues on the control of Meloidogyne incognita and genomic studies of the biocontrol agent Bacillus velezensis. 2020. 86 p. Tese (Doutorado em Agronomia/Fitopatologia)-Universidade Federal de Lavras, Lavras, 2020.
url http://repositorio.ufla.br/jspui/handle/1/41083
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Lavras
Programa de Pós-Graduação em Agronomia/Fitopatologia
UFLA
brasil
Departamento de Fitopatologia
publisher.none.fl_str_mv Universidade Federal de Lavras
Programa de Pós-Graduação em Agronomia/Fitopatologia
UFLA
brasil
Departamento de Fitopatologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1815439139549478912