Genetic diversity between and within populations of Handroanthus heptaphyllus (Vell.) Mattos using microsatellite markers

Detalhes bibliográficos
Autor(a) principal: Mori, Neide Tomita
Data de Publicação: 2016
Outros Autores: Moraes, Mario Luiz Teixeira de, Morita, Caroline Midori, Mori, Edson Seizo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/14262
Resumo: Handroanthus heptaphyllus (Vell.) Mattos, popularly known as ipê-roxo, is a species of the family Bignoneaceae much appreciated for its beauty, excellent quality wood which is used for making medicinal products and also in reforestation programs of degraded areas, as well as landscaping and restoration. The aim of this study was to investigate the genetic diversity between and within populations of H. heptaphyllus using microsatellite markers. The 192 seedlings were produced from seeds collected on 30 trees into the two populations of natural forest fragments in Botucatu region, São Paulo, Brazil. Eight microsatellite loci were analyzed, with allelic polymorphism varying from six alleles for locus TAU22 to 14 alleles for loci TAU12, TAU30, and TAU31, with an expected mean number of alleles per locus ( e A ) of 4.9. The mean expected heterozygosity ( e H ) for the two populations was 0.785, the mean observed heterozygosity ( o H ) was 0.609, and the fi xation index (F) was low between populations, with a mean of 0.222. The gene differentiation between the two populations ( ST G ’) was 0.100. We concluded that the higher genetic diversity is within populations; therefore, as far as germplasm collection programs in Botucatu region are concerned, it is recommended that a larger sampling of individuals should be considered within populations, thereby providing good genetic representativeness. The populations have enough genetic diversity to support genetic improvement and germplasm preservation programs.
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spelling Genetic diversity between and within populations of Handroanthus heptaphyllus (Vell.) Mattos using microsatellite markersDiversidade genética entre e dentro de populações de Handroanthus heptaphyllus (Vell.) Mattos por marcadores microssatélitesGermplasmMolecular markersGenetic diversityGermoplasmaMarcadores molecularesDiversidade genéticaHandroanthus heptaphyllus (Vell.) Mattos, popularly known as ipê-roxo, is a species of the family Bignoneaceae much appreciated for its beauty, excellent quality wood which is used for making medicinal products and also in reforestation programs of degraded areas, as well as landscaping and restoration. The aim of this study was to investigate the genetic diversity between and within populations of H. heptaphyllus using microsatellite markers. The 192 seedlings were produced from seeds collected on 30 trees into the two populations of natural forest fragments in Botucatu region, São Paulo, Brazil. Eight microsatellite loci were analyzed, with allelic polymorphism varying from six alleles for locus TAU22 to 14 alleles for loci TAU12, TAU30, and TAU31, with an expected mean number of alleles per locus ( e A ) of 4.9. The mean expected heterozygosity ( e H ) for the two populations was 0.785, the mean observed heterozygosity ( o H ) was 0.609, and the fi xation index (F) was low between populations, with a mean of 0.222. The gene differentiation between the two populations ( ST G ’) was 0.100. We concluded that the higher genetic diversity is within populations; therefore, as far as germplasm collection programs in Botucatu region are concerned, it is recommended that a larger sampling of individuals should be considered within populations, thereby providing good genetic representativeness. The populations have enough genetic diversity to support genetic improvement and germplasm preservation programs.Handroanthus heptaphyllus (Vell.) Mattos, popularmente conhecida por ipê-roxo, é uma espécie pertencente à família Bignoneaceae, muito apreciada por sua beleza, madeira de excelente qualidade e utilizada em produtos medicinais e programas de reflorestamento de áreas degradadas, paisagismo e restauração. Neste trabalho, objetivou-se estudar a diversidade genética entre e dentro das populações de H. heptaphyllus por meio de marcadores microssatélites. Foram estudadas 192 plântulas, formadas a partir de sementes colhidas de duas populações, em um total de 30 árvores, de fragmentos florestais naturais na região de Botucatu - SP. Foram analisados oito locos microssatélites, com polimorfismo alélico, variando de seis alelos para o loco TAU22 a 14 alelos para os locos TAU12, TAU30 e TAU31, com número efetivo médio de alelos por loco ( e A ) igual a 4,9. As médias para a heterozigosidade esperada ( e H ), para as duas populações foi de 0,785, a heterozigosidade observada ( o H ) foi de 0,609 e o índice de fixação (F) variou pouco entre as populações, com média de 0,222. O valor médio da divergência genética entre as duas populações (ST G’) foi de 0,100. Conclui-se que a maior diversidade genética ocorre dentro das populações; portanto, em programa de coleta de germoplasma, para a região de Botucatu, é recomendado realizar uma maior amostragem de indivíduos dentro das populações, o que possibilitaria uma boa representatividade genética. As populações estudadas possuem diversidade genética para subsidiar programas de melhoramento genético e conservação de germoplasma.Universidade Federal de Lavras (UFLA)2016-04-042017-08-01T20:14:04Z2017-08-01T20:14:04Z2017-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfMORI, N. T. et al. Genetic diversity between and within populations of Handroanthus heptaphyllus (Vell.) Mattos using microsatellite markers. CERNE, Lavras, v. 18, n. 1, p. 9-15, jan./mar. 2012.http://repositorio.ufla.br/jspui/handle/1/142622317-63420104-7760reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAenghttp://www.cerne.ufla.br/site/index.php/CERNE/article/view/793/575Copyright (c) 2016 CERNEhttp://creativecommons.org/licenses/by/4.0/Attribution 4.0 Internationalinfo:eu-repo/semantics/openAccessMori, Neide TomitaMoraes, Mario Luiz Teixeira deMorita, Caroline MidoriMori, Edson Seizo2021-06-07T00:33:55Zoai:localhost:1/14262Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2021-06-07T00:33:55Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Genetic diversity between and within populations of Handroanthus heptaphyllus (Vell.) Mattos using microsatellite markers
Diversidade genética entre e dentro de populações de Handroanthus heptaphyllus (Vell.) Mattos por marcadores microssatélites
title Genetic diversity between and within populations of Handroanthus heptaphyllus (Vell.) Mattos using microsatellite markers
spellingShingle Genetic diversity between and within populations of Handroanthus heptaphyllus (Vell.) Mattos using microsatellite markers
Mori, Neide Tomita
Germplasm
Molecular markers
Genetic diversity
Germoplasma
Marcadores moleculares
Diversidade genética
title_short Genetic diversity between and within populations of Handroanthus heptaphyllus (Vell.) Mattos using microsatellite markers
title_full Genetic diversity between and within populations of Handroanthus heptaphyllus (Vell.) Mattos using microsatellite markers
title_fullStr Genetic diversity between and within populations of Handroanthus heptaphyllus (Vell.) Mattos using microsatellite markers
title_full_unstemmed Genetic diversity between and within populations of Handroanthus heptaphyllus (Vell.) Mattos using microsatellite markers
title_sort Genetic diversity between and within populations of Handroanthus heptaphyllus (Vell.) Mattos using microsatellite markers
author Mori, Neide Tomita
author_facet Mori, Neide Tomita
Moraes, Mario Luiz Teixeira de
Morita, Caroline Midori
Mori, Edson Seizo
author_role author
author2 Moraes, Mario Luiz Teixeira de
Morita, Caroline Midori
Mori, Edson Seizo
author2_role author
author
author
dc.contributor.author.fl_str_mv Mori, Neide Tomita
Moraes, Mario Luiz Teixeira de
Morita, Caroline Midori
Mori, Edson Seizo
dc.subject.por.fl_str_mv Germplasm
Molecular markers
Genetic diversity
Germoplasma
Marcadores moleculares
Diversidade genética
topic Germplasm
Molecular markers
Genetic diversity
Germoplasma
Marcadores moleculares
Diversidade genética
description Handroanthus heptaphyllus (Vell.) Mattos, popularly known as ipê-roxo, is a species of the family Bignoneaceae much appreciated for its beauty, excellent quality wood which is used for making medicinal products and also in reforestation programs of degraded areas, as well as landscaping and restoration. The aim of this study was to investigate the genetic diversity between and within populations of H. heptaphyllus using microsatellite markers. The 192 seedlings were produced from seeds collected on 30 trees into the two populations of natural forest fragments in Botucatu region, São Paulo, Brazil. Eight microsatellite loci were analyzed, with allelic polymorphism varying from six alleles for locus TAU22 to 14 alleles for loci TAU12, TAU30, and TAU31, with an expected mean number of alleles per locus ( e A ) of 4.9. The mean expected heterozygosity ( e H ) for the two populations was 0.785, the mean observed heterozygosity ( o H ) was 0.609, and the fi xation index (F) was low between populations, with a mean of 0.222. The gene differentiation between the two populations ( ST G ’) was 0.100. We concluded that the higher genetic diversity is within populations; therefore, as far as germplasm collection programs in Botucatu region are concerned, it is recommended that a larger sampling of individuals should be considered within populations, thereby providing good genetic representativeness. The populations have enough genetic diversity to support genetic improvement and germplasm preservation programs.
publishDate 2016
dc.date.none.fl_str_mv 2016-04-04
2017-08-01T20:14:04Z
2017-08-01T20:14:04Z
2017-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv MORI, N. T. et al. Genetic diversity between and within populations of Handroanthus heptaphyllus (Vell.) Mattos using microsatellite markers. CERNE, Lavras, v. 18, n. 1, p. 9-15, jan./mar. 2012.
http://repositorio.ufla.br/jspui/handle/1/14262
identifier_str_mv MORI, N. T. et al. Genetic diversity between and within populations of Handroanthus heptaphyllus (Vell.) Mattos using microsatellite markers. CERNE, Lavras, v. 18, n. 1, p. 9-15, jan./mar. 2012.
url http://repositorio.ufla.br/jspui/handle/1/14262
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.cerne.ufla.br/site/index.php/CERNE/article/view/793/575
dc.rights.driver.fl_str_mv Copyright (c) 2016 CERNE
http://creativecommons.org/licenses/by/4.0/
Attribution 4.0 International
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2016 CERNE
http://creativecommons.org/licenses/by/4.0/
Attribution 4.0 International
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Lavras (UFLA)
publisher.none.fl_str_mv Universidade Federal de Lavras (UFLA)
dc.source.none.fl_str_mv 2317-6342
0104-7760
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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