Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco

Detalhes bibliográficos
Autor(a) principal: Lemos, Roxane do Carmo
Data de Publicação: 2021
Tipo de documento: Tese
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/46351
Resumo: In some species, the most used alternative to accelerate the process of obtaining lines is by using doubled haploids (DH). The existing question is whether the process of induction and duplication of chromosomes occurs randomly or there is a restriction in the segregation. If this fact occurs, it would be a restriction on its use since the variability generated in the crossings would not be fully explored. This work was carried out with the purpose to compare the performance of tobacco lines (Nicotiana tabacum L.) obtained by conventional breeding method (CM), with the obtained by the DH methodology, using anther culture. Additionally, to verify the feasibility of routine employment of DH in a recurrent selection program (RS). For this purpose, three populations of tobacco from the Virginia varietal group were obtained by biparental crossing. Considering all populations, the number of lines evaluated was 190 DH and 194 CM. They were evaluated simultaneously, in one experiment per population at two locations. The traits assessed were green leaf yield (YLD), total alkaloid content (ALK), and sugar content (SU). The genetic and the phenotypic parameters were estimated by population. It was found that: the performance of the DH in comparison to CM differed between the traits. For those related to quality, ALK and SU, the performance was similar. For YLD, the genetic variation estimates and h2, among the DH was higher than those of CM. However, the opposite occurred for the mean parameter. Considering all lines, regardless of the crossing, the CM mean was 13.3% higher than the DH mean, for YLD. Also, the annual gain with the selection was greater for CM to this trait. The use of DH in a SR program will be efficient in comparison to CM if a large number of DH are obtained which must undergo a preliminary selection before the most intensive evaluation to identify the lines to be recombined.
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spelling Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobaccoDesempenho de linhagens duplo-haploides em relação às obtidas pelo método convencional de melhoramento no tabacoTabaco - Melhoramento genéticoTabaco - Duplo haplóidesSeleção recorrenteNicotiana tabacum L.Tobacco - Plant breedingTobacco - Double haploidsRecurring selectionMelhoramento VegetalIn some species, the most used alternative to accelerate the process of obtaining lines is by using doubled haploids (DH). The existing question is whether the process of induction and duplication of chromosomes occurs randomly or there is a restriction in the segregation. If this fact occurs, it would be a restriction on its use since the variability generated in the crossings would not be fully explored. This work was carried out with the purpose to compare the performance of tobacco lines (Nicotiana tabacum L.) obtained by conventional breeding method (CM), with the obtained by the DH methodology, using anther culture. Additionally, to verify the feasibility of routine employment of DH in a recurrent selection program (RS). For this purpose, three populations of tobacco from the Virginia varietal group were obtained by biparental crossing. Considering all populations, the number of lines evaluated was 190 DH and 194 CM. They were evaluated simultaneously, in one experiment per population at two locations. The traits assessed were green leaf yield (YLD), total alkaloid content (ALK), and sugar content (SU). The genetic and the phenotypic parameters were estimated by population. It was found that: the performance of the DH in comparison to CM differed between the traits. For those related to quality, ALK and SU, the performance was similar. For YLD, the genetic variation estimates and h2, among the DH was higher than those of CM. However, the opposite occurred for the mean parameter. Considering all lines, regardless of the crossing, the CM mean was 13.3% higher than the DH mean, for YLD. Also, the annual gain with the selection was greater for CM to this trait. The use of DH in a SR program will be efficient in comparison to CM if a large number of DH are obtained which must undergo a preliminary selection before the most intensive evaluation to identify the lines to be recombined.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Em algumas espécies, a alternativa mais utilizada para acelerar o processo de obtenção de linhagens é a utilização de duplo haploides (DH). A dúvida existente é se o processo de indução e duplicação dos cromossomos ocorre de forma aleatória ou se há restrição na segregação. Caso isso ocorra, seria uma limitação ao seu uso, uma vez que a variabilidade gerada nos cruzamentos não seria totalmente explorada. Este trabalho foi realizado com o objetivo de comparar o desempenho de linhagens de tabaco (Nicotiana tabacum L.) obtidas pelo método convencional de melhoramento (CM), com as obtidas pela metodologia DH por meio de cultura de anteras. Além disso, verificar a viabilidade do emprego rotineiro de DH em um programa de seleção recorrente (RS). Para isso, três populações de tabaco do grupo varietal Virginia foram obtidas por cruzamento biparental. Considerando todas as populações, o número de linhagens avaliadas foi de 190 DH e 194 CM. Elas foram avaliadas simultaneamente, em um experimento por população em dois locais. As características avaliadas foram produtividade de massa verde (YLD), teor de alcaloides totais (ALK) e teor de açúcar (SU). Os parâmetros genéticos e fenotípicos foram estimados por população e considerando todas as linhagens independente do cruzamento. Constatou-se que o desempenho do DH em comparação ao CM diferiu entre as características. Para aqueles relacionados à qualidade, ALK e SU, o desempenho foi semelhante. Para YLD, as estimativas de variação genética e h2 entre os DH foram maiores que as de CM. No entanto, o oposto ocorreu para a média. Considerando todas as linhagens, independente do cruzamento, a média do CM foi 13,3% maior do que a média do DH, para YLD. Além disso, o ganho anual com a seleção foi maior para CM para esta característica. O uso de DH em um programa de SR será eficiente em comparação ao MC se for obtido um grande número de DH que deve passar por uma seleção preliminar antes da avaliação mais intensiva para identificar as linhagens a serem recombinadas.Universidade Federal de LavrasPrograma de Pós-Graduação em Genética e Melhoramento de PlantasUFLAbrasilDepartamento de BiologiaRamalho, Magno Antonio PattoCarvalho, Bruna LineRezende, Camila Bastos RibeiroBerger, Irving JosephPádua, José Maria VilellaLemos, Roxane do Carmo2021-05-24T17:29:34Z2021-05-24T17:29:34Z2021-05-242021-04-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfLEMOS, R. do C. Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco. 2021. 63 p. Tese (Doutorado em Genética e Melhoramento de Plantas) – Universidade Federal de Lavras, Lavras, 2021.http://repositorio.ufla.br/jspui/handle/1/46351enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2021-05-24T17:29:34Zoai:localhost:1/46351Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2021-05-24T17:29:34Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco
Desempenho de linhagens duplo-haploides em relação às obtidas pelo método convencional de melhoramento no tabaco
title Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco
spellingShingle Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco
Lemos, Roxane do Carmo
Tabaco - Melhoramento genético
Tabaco - Duplo haplóides
Seleção recorrente
Nicotiana tabacum L.
Tobacco - Plant breeding
Tobacco - Double haploids
Recurring selection
Melhoramento Vegetal
title_short Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco
title_full Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco
title_fullStr Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco
title_full_unstemmed Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco
title_sort Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco
author Lemos, Roxane do Carmo
author_facet Lemos, Roxane do Carmo
author_role author
dc.contributor.none.fl_str_mv Ramalho, Magno Antonio Patto
Carvalho, Bruna Line
Rezende, Camila Bastos Ribeiro
Berger, Irving Joseph
Pádua, José Maria Vilella
dc.contributor.author.fl_str_mv Lemos, Roxane do Carmo
dc.subject.por.fl_str_mv Tabaco - Melhoramento genético
Tabaco - Duplo haplóides
Seleção recorrente
Nicotiana tabacum L.
Tobacco - Plant breeding
Tobacco - Double haploids
Recurring selection
Melhoramento Vegetal
topic Tabaco - Melhoramento genético
Tabaco - Duplo haplóides
Seleção recorrente
Nicotiana tabacum L.
Tobacco - Plant breeding
Tobacco - Double haploids
Recurring selection
Melhoramento Vegetal
description In some species, the most used alternative to accelerate the process of obtaining lines is by using doubled haploids (DH). The existing question is whether the process of induction and duplication of chromosomes occurs randomly or there is a restriction in the segregation. If this fact occurs, it would be a restriction on its use since the variability generated in the crossings would not be fully explored. This work was carried out with the purpose to compare the performance of tobacco lines (Nicotiana tabacum L.) obtained by conventional breeding method (CM), with the obtained by the DH methodology, using anther culture. Additionally, to verify the feasibility of routine employment of DH in a recurrent selection program (RS). For this purpose, three populations of tobacco from the Virginia varietal group were obtained by biparental crossing. Considering all populations, the number of lines evaluated was 190 DH and 194 CM. They were evaluated simultaneously, in one experiment per population at two locations. The traits assessed were green leaf yield (YLD), total alkaloid content (ALK), and sugar content (SU). The genetic and the phenotypic parameters were estimated by population. It was found that: the performance of the DH in comparison to CM differed between the traits. For those related to quality, ALK and SU, the performance was similar. For YLD, the genetic variation estimates and h2, among the DH was higher than those of CM. However, the opposite occurred for the mean parameter. Considering all lines, regardless of the crossing, the CM mean was 13.3% higher than the DH mean, for YLD. Also, the annual gain with the selection was greater for CM to this trait. The use of DH in a SR program will be efficient in comparison to CM if a large number of DH are obtained which must undergo a preliminary selection before the most intensive evaluation to identify the lines to be recombined.
publishDate 2021
dc.date.none.fl_str_mv 2021-05-24T17:29:34Z
2021-05-24T17:29:34Z
2021-05-24
2021-04-16
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv LEMOS, R. do C. Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco. 2021. 63 p. Tese (Doutorado em Genética e Melhoramento de Plantas) – Universidade Federal de Lavras, Lavras, 2021.
http://repositorio.ufla.br/jspui/handle/1/46351
identifier_str_mv LEMOS, R. do C. Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco. 2021. 63 p. Tese (Doutorado em Genética e Melhoramento de Plantas) – Universidade Federal de Lavras, Lavras, 2021.
url http://repositorio.ufla.br/jspui/handle/1/46351
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Lavras
Programa de Pós-Graduação em Genética e Melhoramento de Plantas
UFLA
brasil
Departamento de Biologia
publisher.none.fl_str_mv Universidade Federal de Lavras
Programa de Pós-Graduação em Genética e Melhoramento de Plantas
UFLA
brasil
Departamento de Biologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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