Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Tese |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/46351 |
Resumo: | In some species, the most used alternative to accelerate the process of obtaining lines is by using doubled haploids (DH). The existing question is whether the process of induction and duplication of chromosomes occurs randomly or there is a restriction in the segregation. If this fact occurs, it would be a restriction on its use since the variability generated in the crossings would not be fully explored. This work was carried out with the purpose to compare the performance of tobacco lines (Nicotiana tabacum L.) obtained by conventional breeding method (CM), with the obtained by the DH methodology, using anther culture. Additionally, to verify the feasibility of routine employment of DH in a recurrent selection program (RS). For this purpose, three populations of tobacco from the Virginia varietal group were obtained by biparental crossing. Considering all populations, the number of lines evaluated was 190 DH and 194 CM. They were evaluated simultaneously, in one experiment per population at two locations. The traits assessed were green leaf yield (YLD), total alkaloid content (ALK), and sugar content (SU). The genetic and the phenotypic parameters were estimated by population. It was found that: the performance of the DH in comparison to CM differed between the traits. For those related to quality, ALK and SU, the performance was similar. For YLD, the genetic variation estimates and h2, among the DH was higher than those of CM. However, the opposite occurred for the mean parameter. Considering all lines, regardless of the crossing, the CM mean was 13.3% higher than the DH mean, for YLD. Also, the annual gain with the selection was greater for CM to this trait. The use of DH in a SR program will be efficient in comparison to CM if a large number of DH are obtained which must undergo a preliminary selection before the most intensive evaluation to identify the lines to be recombined. |
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Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobaccoDesempenho de linhagens duplo-haploides em relação às obtidas pelo método convencional de melhoramento no tabacoTabaco - Melhoramento genéticoTabaco - Duplo haplóidesSeleção recorrenteNicotiana tabacum L.Tobacco - Plant breedingTobacco - Double haploidsRecurring selectionMelhoramento VegetalIn some species, the most used alternative to accelerate the process of obtaining lines is by using doubled haploids (DH). The existing question is whether the process of induction and duplication of chromosomes occurs randomly or there is a restriction in the segregation. If this fact occurs, it would be a restriction on its use since the variability generated in the crossings would not be fully explored. This work was carried out with the purpose to compare the performance of tobacco lines (Nicotiana tabacum L.) obtained by conventional breeding method (CM), with the obtained by the DH methodology, using anther culture. Additionally, to verify the feasibility of routine employment of DH in a recurrent selection program (RS). For this purpose, three populations of tobacco from the Virginia varietal group were obtained by biparental crossing. Considering all populations, the number of lines evaluated was 190 DH and 194 CM. They were evaluated simultaneously, in one experiment per population at two locations. The traits assessed were green leaf yield (YLD), total alkaloid content (ALK), and sugar content (SU). The genetic and the phenotypic parameters were estimated by population. It was found that: the performance of the DH in comparison to CM differed between the traits. For those related to quality, ALK and SU, the performance was similar. For YLD, the genetic variation estimates and h2, among the DH was higher than those of CM. However, the opposite occurred for the mean parameter. Considering all lines, regardless of the crossing, the CM mean was 13.3% higher than the DH mean, for YLD. Also, the annual gain with the selection was greater for CM to this trait. The use of DH in a SR program will be efficient in comparison to CM if a large number of DH are obtained which must undergo a preliminary selection before the most intensive evaluation to identify the lines to be recombined.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Em algumas espécies, a alternativa mais utilizada para acelerar o processo de obtenção de linhagens é a utilização de duplo haploides (DH). A dúvida existente é se o processo de indução e duplicação dos cromossomos ocorre de forma aleatória ou se há restrição na segregação. Caso isso ocorra, seria uma limitação ao seu uso, uma vez que a variabilidade gerada nos cruzamentos não seria totalmente explorada. Este trabalho foi realizado com o objetivo de comparar o desempenho de linhagens de tabaco (Nicotiana tabacum L.) obtidas pelo método convencional de melhoramento (CM), com as obtidas pela metodologia DH por meio de cultura de anteras. Além disso, verificar a viabilidade do emprego rotineiro de DH em um programa de seleção recorrente (RS). Para isso, três populações de tabaco do grupo varietal Virginia foram obtidas por cruzamento biparental. Considerando todas as populações, o número de linhagens avaliadas foi de 190 DH e 194 CM. Elas foram avaliadas simultaneamente, em um experimento por população em dois locais. As características avaliadas foram produtividade de massa verde (YLD), teor de alcaloides totais (ALK) e teor de açúcar (SU). Os parâmetros genéticos e fenotípicos foram estimados por população e considerando todas as linhagens independente do cruzamento. Constatou-se que o desempenho do DH em comparação ao CM diferiu entre as características. Para aqueles relacionados à qualidade, ALK e SU, o desempenho foi semelhante. Para YLD, as estimativas de variação genética e h2 entre os DH foram maiores que as de CM. No entanto, o oposto ocorreu para a média. Considerando todas as linhagens, independente do cruzamento, a média do CM foi 13,3% maior do que a média do DH, para YLD. Além disso, o ganho anual com a seleção foi maior para CM para esta característica. O uso de DH em um programa de SR será eficiente em comparação ao MC se for obtido um grande número de DH que deve passar por uma seleção preliminar antes da avaliação mais intensiva para identificar as linhagens a serem recombinadas.Universidade Federal de LavrasPrograma de Pós-Graduação em Genética e Melhoramento de PlantasUFLAbrasilDepartamento de BiologiaRamalho, Magno Antonio PattoCarvalho, Bruna LineRezende, Camila Bastos RibeiroBerger, Irving JosephPádua, José Maria VilellaLemos, Roxane do Carmo2021-05-24T17:29:34Z2021-05-24T17:29:34Z2021-05-242021-04-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfLEMOS, R. do C. Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco. 2021. 63 p. Tese (Doutorado em Genética e Melhoramento de Plantas) – Universidade Federal de Lavras, Lavras, 2021.http://repositorio.ufla.br/jspui/handle/1/46351enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2021-05-24T17:29:34Zoai:localhost:1/46351Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2021-05-24T17:29:34Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco Desempenho de linhagens duplo-haploides em relação às obtidas pelo método convencional de melhoramento no tabaco |
title |
Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco |
spellingShingle |
Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco Lemos, Roxane do Carmo Tabaco - Melhoramento genético Tabaco - Duplo haplóides Seleção recorrente Nicotiana tabacum L. Tobacco - Plant breeding Tobacco - Double haploids Recurring selection Melhoramento Vegetal |
title_short |
Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco |
title_full |
Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco |
title_fullStr |
Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco |
title_full_unstemmed |
Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco |
title_sort |
Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco |
author |
Lemos, Roxane do Carmo |
author_facet |
Lemos, Roxane do Carmo |
author_role |
author |
dc.contributor.none.fl_str_mv |
Ramalho, Magno Antonio Patto Carvalho, Bruna Line Rezende, Camila Bastos Ribeiro Berger, Irving Joseph Pádua, José Maria Vilella |
dc.contributor.author.fl_str_mv |
Lemos, Roxane do Carmo |
dc.subject.por.fl_str_mv |
Tabaco - Melhoramento genético Tabaco - Duplo haplóides Seleção recorrente Nicotiana tabacum L. Tobacco - Plant breeding Tobacco - Double haploids Recurring selection Melhoramento Vegetal |
topic |
Tabaco - Melhoramento genético Tabaco - Duplo haplóides Seleção recorrente Nicotiana tabacum L. Tobacco - Plant breeding Tobacco - Double haploids Recurring selection Melhoramento Vegetal |
description |
In some species, the most used alternative to accelerate the process of obtaining lines is by using doubled haploids (DH). The existing question is whether the process of induction and duplication of chromosomes occurs randomly or there is a restriction in the segregation. If this fact occurs, it would be a restriction on its use since the variability generated in the crossings would not be fully explored. This work was carried out with the purpose to compare the performance of tobacco lines (Nicotiana tabacum L.) obtained by conventional breeding method (CM), with the obtained by the DH methodology, using anther culture. Additionally, to verify the feasibility of routine employment of DH in a recurrent selection program (RS). For this purpose, three populations of tobacco from the Virginia varietal group were obtained by biparental crossing. Considering all populations, the number of lines evaluated was 190 DH and 194 CM. They were evaluated simultaneously, in one experiment per population at two locations. The traits assessed were green leaf yield (YLD), total alkaloid content (ALK), and sugar content (SU). The genetic and the phenotypic parameters were estimated by population. It was found that: the performance of the DH in comparison to CM differed between the traits. For those related to quality, ALK and SU, the performance was similar. For YLD, the genetic variation estimates and h2, among the DH was higher than those of CM. However, the opposite occurred for the mean parameter. Considering all lines, regardless of the crossing, the CM mean was 13.3% higher than the DH mean, for YLD. Also, the annual gain with the selection was greater for CM to this trait. The use of DH in a SR program will be efficient in comparison to CM if a large number of DH are obtained which must undergo a preliminary selection before the most intensive evaluation to identify the lines to be recombined. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-05-24T17:29:34Z 2021-05-24T17:29:34Z 2021-05-24 2021-04-16 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
LEMOS, R. do C. Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco. 2021. 63 p. Tese (Doutorado em Genética e Melhoramento de Plantas) – Universidade Federal de Lavras, Lavras, 2021. http://repositorio.ufla.br/jspui/handle/1/46351 |
identifier_str_mv |
LEMOS, R. do C. Performance of doubled haploid lines in relation to those obtained by the conventional breeding method in tobacco. 2021. 63 p. Tese (Doutorado em Genética e Melhoramento de Plantas) – Universidade Federal de Lavras, Lavras, 2021. |
url |
http://repositorio.ufla.br/jspui/handle/1/46351 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Genética e Melhoramento de Plantas UFLA brasil Departamento de Biologia |
publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Genética e Melhoramento de Plantas UFLA brasil Departamento de Biologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1815439067035205632 |