Occurrence of Pseudomonas syringae pv. garcae in coffee seeds
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/29979 |
Resumo: | Given the damage and losses caused by bacterial blight of coffee (Pseudomonas syringae pv. garcae) (PSG) in coffee (Coffea arabica) seedling nurseries and lack of knowledge of the origin of the initial inoculum, the goal of this study was to determine whether coffee seeds can contain viable inoculum and spread bacterial inoculum. Seeds from plants with symptoms of bacterial blight of coffee were immersed in sterilized saline solution, and the crude extract obtained was inoculated in leaves of coffee seedlings. Leaves of plants inoculated with seed extract from symptomatic plants had symptoms of bacterial blight similar to those on leaves inoculated with reference isolate PSG. Seedlings inoculated with seed extract from asymptomatic plants showed no symptoms. Symptomatic leaves were submitted to exudate tests, and the etiologic agent was isolated in culture medium. The isolates with colonies morphologically similar to reference isolate PSG were selected and tested for hypersensitive reaction (HR) and pathogenicity to coffee seedlings. The isolates positive for HR and pathogenic to coffee also showed biochemical and molecular patterns equal to those of reference isolate PSG by the rep-PCR technique using Box primer. Thus, coffee seeds from plants with symptoms of bacterial blight can contain viable inoculum of P. syringae pv. garcae. |
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Occurrence of Pseudomonas syringae pv. garcae in coffee seedsBacterial blightCoffea arabicaInitial inoculumSeedling nurserySeed healthFerrugem bacterianaInóculo inicialViveiro de mudasSaúde das sementesGiven the damage and losses caused by bacterial blight of coffee (Pseudomonas syringae pv. garcae) (PSG) in coffee (Coffea arabica) seedling nurseries and lack of knowledge of the origin of the initial inoculum, the goal of this study was to determine whether coffee seeds can contain viable inoculum and spread bacterial inoculum. Seeds from plants with symptoms of bacterial blight of coffee were immersed in sterilized saline solution, and the crude extract obtained was inoculated in leaves of coffee seedlings. Leaves of plants inoculated with seed extract from symptomatic plants had symptoms of bacterial blight similar to those on leaves inoculated with reference isolate PSG. Seedlings inoculated with seed extract from asymptomatic plants showed no symptoms. Symptomatic leaves were submitted to exudate tests, and the etiologic agent was isolated in culture medium. The isolates with colonies morphologically similar to reference isolate PSG were selected and tested for hypersensitive reaction (HR) and pathogenicity to coffee seedlings. The isolates positive for HR and pathogenic to coffee also showed biochemical and molecular patterns equal to those of reference isolate PSG by the rep-PCR technique using Box primer. Thus, coffee seeds from plants with symptoms of bacterial blight can contain viable inoculum of P. syringae pv. garcae.Southern Cross Publishing2018-08-14T19:42:44Z2018-08-14T19:42:44Z2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBELAN, L. L. et al. Occurrence of Pseudomonas syringae pv. garcae in coffee seeds. Australian Journal of Crop Science, [S. l.], v. 10, n. 7, p. 1015-1021, July 2016.http://repositorio.ufla.br/jspui/handle/1/29979Australian Journal of Crop Sciencereponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessBelan, Leônidas LeoniPozza, Edson AmpélioFreitas, Marcelo Loran de OliveiraRaimundi, Melina KorresSouza, Ricardo Magela deMachado, José da Cruzeng2023-05-26T19:51:20Zoai:localhost:1/29979Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T19:51:20Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Occurrence of Pseudomonas syringae pv. garcae in coffee seeds |
title |
Occurrence of Pseudomonas syringae pv. garcae in coffee seeds |
spellingShingle |
Occurrence of Pseudomonas syringae pv. garcae in coffee seeds Belan, Leônidas Leoni Bacterial blight Coffea arabica Initial inoculum Seedling nursery Seed health Ferrugem bacteriana Inóculo inicial Viveiro de mudas Saúde das sementes |
title_short |
Occurrence of Pseudomonas syringae pv. garcae in coffee seeds |
title_full |
Occurrence of Pseudomonas syringae pv. garcae in coffee seeds |
title_fullStr |
Occurrence of Pseudomonas syringae pv. garcae in coffee seeds |
title_full_unstemmed |
Occurrence of Pseudomonas syringae pv. garcae in coffee seeds |
title_sort |
Occurrence of Pseudomonas syringae pv. garcae in coffee seeds |
author |
Belan, Leônidas Leoni |
author_facet |
Belan, Leônidas Leoni Pozza, Edson Ampélio Freitas, Marcelo Loran de Oliveira Raimundi, Melina Korres Souza, Ricardo Magela de Machado, José da Cruz |
author_role |
author |
author2 |
Pozza, Edson Ampélio Freitas, Marcelo Loran de Oliveira Raimundi, Melina Korres Souza, Ricardo Magela de Machado, José da Cruz |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Belan, Leônidas Leoni Pozza, Edson Ampélio Freitas, Marcelo Loran de Oliveira Raimundi, Melina Korres Souza, Ricardo Magela de Machado, José da Cruz |
dc.subject.por.fl_str_mv |
Bacterial blight Coffea arabica Initial inoculum Seedling nursery Seed health Ferrugem bacteriana Inóculo inicial Viveiro de mudas Saúde das sementes |
topic |
Bacterial blight Coffea arabica Initial inoculum Seedling nursery Seed health Ferrugem bacteriana Inóculo inicial Viveiro de mudas Saúde das sementes |
description |
Given the damage and losses caused by bacterial blight of coffee (Pseudomonas syringae pv. garcae) (PSG) in coffee (Coffea arabica) seedling nurseries and lack of knowledge of the origin of the initial inoculum, the goal of this study was to determine whether coffee seeds can contain viable inoculum and spread bacterial inoculum. Seeds from plants with symptoms of bacterial blight of coffee were immersed in sterilized saline solution, and the crude extract obtained was inoculated in leaves of coffee seedlings. Leaves of plants inoculated with seed extract from symptomatic plants had symptoms of bacterial blight similar to those on leaves inoculated with reference isolate PSG. Seedlings inoculated with seed extract from asymptomatic plants showed no symptoms. Symptomatic leaves were submitted to exudate tests, and the etiologic agent was isolated in culture medium. The isolates with colonies morphologically similar to reference isolate PSG were selected and tested for hypersensitive reaction (HR) and pathogenicity to coffee seedlings. The isolates positive for HR and pathogenic to coffee also showed biochemical and molecular patterns equal to those of reference isolate PSG by the rep-PCR technique using Box primer. Thus, coffee seeds from plants with symptoms of bacterial blight can contain viable inoculum of P. syringae pv. garcae. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2018-08-14T19:42:44Z 2018-08-14T19:42:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
BELAN, L. L. et al. Occurrence of Pseudomonas syringae pv. garcae in coffee seeds. Australian Journal of Crop Science, [S. l.], v. 10, n. 7, p. 1015-1021, July 2016. http://repositorio.ufla.br/jspui/handle/1/29979 |
identifier_str_mv |
BELAN, L. L. et al. Occurrence of Pseudomonas syringae pv. garcae in coffee seeds. Australian Journal of Crop Science, [S. l.], v. 10, n. 7, p. 1015-1021, July 2016. |
url |
http://repositorio.ufla.br/jspui/handle/1/29979 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Southern Cross Publishing |
publisher.none.fl_str_mv |
Southern Cross Publishing |
dc.source.none.fl_str_mv |
Australian Journal of Crop Science reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1815439366035603456 |