Occurrence of Pseudomonas syringae pv. garcae in coffee seeds

Detalhes bibliográficos
Autor(a) principal: Belan, Leônidas Leoni
Data de Publicação: 2016
Outros Autores: Pozza, Edson Ampélio, Freitas, Marcelo Loran de Oliveira, Raimundi, Melina Korres, Souza, Ricardo Magela de, Machado, José da Cruz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/29979
Resumo: Given the damage and losses caused by bacterial blight of coffee (Pseudomonas syringae pv. garcae) (PSG) in coffee (Coffea arabica) seedling nurseries and lack of knowledge of the origin of the initial inoculum, the goal of this study was to determine whether coffee seeds can contain viable inoculum and spread bacterial inoculum. Seeds from plants with symptoms of bacterial blight of coffee were immersed in sterilized saline solution, and the crude extract obtained was inoculated in leaves of coffee seedlings. Leaves of plants inoculated with seed extract from symptomatic plants had symptoms of bacterial blight similar to those on leaves inoculated with reference isolate PSG. Seedlings inoculated with seed extract from asymptomatic plants showed no symptoms. Symptomatic leaves were submitted to exudate tests, and the etiologic agent was isolated in culture medium. The isolates with colonies morphologically similar to reference isolate PSG were selected and tested for hypersensitive reaction (HR) and pathogenicity to coffee seedlings. The isolates positive for HR and pathogenic to coffee also showed biochemical and molecular patterns equal to those of reference isolate PSG by the rep-PCR technique using Box primer. Thus, coffee seeds from plants with symptoms of bacterial blight can contain viable inoculum of P. syringae pv. garcae.
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spelling Occurrence of Pseudomonas syringae pv. garcae in coffee seedsBacterial blightCoffea arabicaInitial inoculumSeedling nurserySeed healthFerrugem bacterianaInóculo inicialViveiro de mudasSaúde das sementesGiven the damage and losses caused by bacterial blight of coffee (Pseudomonas syringae pv. garcae) (PSG) in coffee (Coffea arabica) seedling nurseries and lack of knowledge of the origin of the initial inoculum, the goal of this study was to determine whether coffee seeds can contain viable inoculum and spread bacterial inoculum. Seeds from plants with symptoms of bacterial blight of coffee were immersed in sterilized saline solution, and the crude extract obtained was inoculated in leaves of coffee seedlings. Leaves of plants inoculated with seed extract from symptomatic plants had symptoms of bacterial blight similar to those on leaves inoculated with reference isolate PSG. Seedlings inoculated with seed extract from asymptomatic plants showed no symptoms. Symptomatic leaves were submitted to exudate tests, and the etiologic agent was isolated in culture medium. The isolates with colonies morphologically similar to reference isolate PSG were selected and tested for hypersensitive reaction (HR) and pathogenicity to coffee seedlings. The isolates positive for HR and pathogenic to coffee also showed biochemical and molecular patterns equal to those of reference isolate PSG by the rep-PCR technique using Box primer. Thus, coffee seeds from plants with symptoms of bacterial blight can contain viable inoculum of P. syringae pv. garcae.Southern Cross Publishing2018-08-14T19:42:44Z2018-08-14T19:42:44Z2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBELAN, L. L. et al. Occurrence of Pseudomonas syringae pv. garcae in coffee seeds. Australian Journal of Crop Science, [S. l.], v. 10, n. 7, p. 1015-1021, July 2016.http://repositorio.ufla.br/jspui/handle/1/29979Australian Journal of Crop Sciencereponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessBelan, Leônidas LeoniPozza, Edson AmpélioFreitas, Marcelo Loran de OliveiraRaimundi, Melina KorresSouza, Ricardo Magela deMachado, José da Cruzeng2023-05-26T19:51:20Zoai:localhost:1/29979Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T19:51:20Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Occurrence of Pseudomonas syringae pv. garcae in coffee seeds
title Occurrence of Pseudomonas syringae pv. garcae in coffee seeds
spellingShingle Occurrence of Pseudomonas syringae pv. garcae in coffee seeds
Belan, Leônidas Leoni
Bacterial blight
Coffea arabica
Initial inoculum
Seedling nursery
Seed health
Ferrugem bacteriana
Inóculo inicial
Viveiro de mudas
Saúde das sementes
title_short Occurrence of Pseudomonas syringae pv. garcae in coffee seeds
title_full Occurrence of Pseudomonas syringae pv. garcae in coffee seeds
title_fullStr Occurrence of Pseudomonas syringae pv. garcae in coffee seeds
title_full_unstemmed Occurrence of Pseudomonas syringae pv. garcae in coffee seeds
title_sort Occurrence of Pseudomonas syringae pv. garcae in coffee seeds
author Belan, Leônidas Leoni
author_facet Belan, Leônidas Leoni
Pozza, Edson Ampélio
Freitas, Marcelo Loran de Oliveira
Raimundi, Melina Korres
Souza, Ricardo Magela de
Machado, José da Cruz
author_role author
author2 Pozza, Edson Ampélio
Freitas, Marcelo Loran de Oliveira
Raimundi, Melina Korres
Souza, Ricardo Magela de
Machado, José da Cruz
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Belan, Leônidas Leoni
Pozza, Edson Ampélio
Freitas, Marcelo Loran de Oliveira
Raimundi, Melina Korres
Souza, Ricardo Magela de
Machado, José da Cruz
dc.subject.por.fl_str_mv Bacterial blight
Coffea arabica
Initial inoculum
Seedling nursery
Seed health
Ferrugem bacteriana
Inóculo inicial
Viveiro de mudas
Saúde das sementes
topic Bacterial blight
Coffea arabica
Initial inoculum
Seedling nursery
Seed health
Ferrugem bacteriana
Inóculo inicial
Viveiro de mudas
Saúde das sementes
description Given the damage and losses caused by bacterial blight of coffee (Pseudomonas syringae pv. garcae) (PSG) in coffee (Coffea arabica) seedling nurseries and lack of knowledge of the origin of the initial inoculum, the goal of this study was to determine whether coffee seeds can contain viable inoculum and spread bacterial inoculum. Seeds from plants with symptoms of bacterial blight of coffee were immersed in sterilized saline solution, and the crude extract obtained was inoculated in leaves of coffee seedlings. Leaves of plants inoculated with seed extract from symptomatic plants had symptoms of bacterial blight similar to those on leaves inoculated with reference isolate PSG. Seedlings inoculated with seed extract from asymptomatic plants showed no symptoms. Symptomatic leaves were submitted to exudate tests, and the etiologic agent was isolated in culture medium. The isolates with colonies morphologically similar to reference isolate PSG were selected and tested for hypersensitive reaction (HR) and pathogenicity to coffee seedlings. The isolates positive for HR and pathogenic to coffee also showed biochemical and molecular patterns equal to those of reference isolate PSG by the rep-PCR technique using Box primer. Thus, coffee seeds from plants with symptoms of bacterial blight can contain viable inoculum of P. syringae pv. garcae.
publishDate 2016
dc.date.none.fl_str_mv 2016
2018-08-14T19:42:44Z
2018-08-14T19:42:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv BELAN, L. L. et al. Occurrence of Pseudomonas syringae pv. garcae in coffee seeds. Australian Journal of Crop Science, [S. l.], v. 10, n. 7, p. 1015-1021, July 2016.
http://repositorio.ufla.br/jspui/handle/1/29979
identifier_str_mv BELAN, L. L. et al. Occurrence of Pseudomonas syringae pv. garcae in coffee seeds. Australian Journal of Crop Science, [S. l.], v. 10, n. 7, p. 1015-1021, July 2016.
url http://repositorio.ufla.br/jspui/handle/1/29979
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Southern Cross Publishing
publisher.none.fl_str_mv Southern Cross Publishing
dc.source.none.fl_str_mv Australian Journal of Crop Science
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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