Heat-resistant protein expression during germination of maize seeds under water stress
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/30234 |
Resumo: | Low water availability is one of the factors that limit agricultural crop development, and hence the development of genotypes with increased water stress tolerance is a challenge in plant breeding programs. Heat-resistant proteins have been widely studied, and are reported to participate in various developmental processes and to accumulate in response to stress. This study aimed to evaluate heat-resistant protein expression under water stress conditions during the germination of maize seed inbreed lines differing in their water stress tolerance. Maize seed lines 91 and 64 were soaked in 0, -0.3, -0.6, and -0.9 MPa water potential for 0, 6, 12, 18, and 24 h. Line 91 is considered more water stress-tolerant than line 64. The analysis of heat-resistant protein expression was made by gel electrophoresis and spectrophotometry. In general, higher expression of heat-resistant proteins was observed in seeds from line 64 subjected to shorter soaking periods and lower water potentials. However, in the water stress-tolerant line 91, a higher expression was observed in seeds that were subjected to -0.3 and -0.6 MPa water potentials. In the absence of water stress, heat-resistant protein expression was reduced with increasing soaking period. Thus, there was a difference in heat-resistant protein expression among the seed lines differing in water stress tolerance. Increased heatresistant protein expression was observed in seeds from line 91 when subjected to water stress conditions for longer soaking periods. |
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Heat-resistant protein expression during germination of maize seeds under water stressZea maysLate embryogenesisProtein abundanceHeat shockLow water availability is one of the factors that limit agricultural crop development, and hence the development of genotypes with increased water stress tolerance is a challenge in plant breeding programs. Heat-resistant proteins have been widely studied, and are reported to participate in various developmental processes and to accumulate in response to stress. This study aimed to evaluate heat-resistant protein expression under water stress conditions during the germination of maize seed inbreed lines differing in their water stress tolerance. Maize seed lines 91 and 64 were soaked in 0, -0.3, -0.6, and -0.9 MPa water potential for 0, 6, 12, 18, and 24 h. Line 91 is considered more water stress-tolerant than line 64. The analysis of heat-resistant protein expression was made by gel electrophoresis and spectrophotometry. In general, higher expression of heat-resistant proteins was observed in seeds from line 64 subjected to shorter soaking periods and lower water potentials. However, in the water stress-tolerant line 91, a higher expression was observed in seeds that were subjected to -0.3 and -0.6 MPa water potentials. In the absence of water stress, heat-resistant protein expression was reduced with increasing soaking period. Thus, there was a difference in heat-resistant protein expression among the seed lines differing in water stress tolerance. Increased heatresistant protein expression was observed in seeds from line 91 when subjected to water stress conditions for longer soaking periods.Fundação de Pesquisas Científicas de Ribeirão Preto2018-08-29T13:31:35Z2018-08-29T13:31:35Z2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfABREU, V. M. et al. Heat-resistant protein expression during germination of maize seeds under water stress. Genetics and Molecular Research, Ribeirão Preto, v. 15, n. 3, 2016.http://repositorio.ufla.br/jspui/handle/1/30234Genetics and Molecular Researchreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-ShareAlike 4.0 Internationalhttp://creativecommons.org/licenses/by-sa/4.0/info:eu-repo/semantics/openAccessAbreu, V. M.Silva Neta, I. C.Von Pinho, Édila Vilela de ResendeNaves, G. M. F.Guimarães, R. M.Santos, H. O.Von Pinho, R. G.eng2018-08-29T13:32:29Zoai:localhost:1/30234Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2018-08-29T13:32:29Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Heat-resistant protein expression during germination of maize seeds under water stress |
title |
Heat-resistant protein expression during germination of maize seeds under water stress |
spellingShingle |
Heat-resistant protein expression during germination of maize seeds under water stress Abreu, V. M. Zea mays Late embryogenesis Protein abundance Heat shock |
title_short |
Heat-resistant protein expression during germination of maize seeds under water stress |
title_full |
Heat-resistant protein expression during germination of maize seeds under water stress |
title_fullStr |
Heat-resistant protein expression during germination of maize seeds under water stress |
title_full_unstemmed |
Heat-resistant protein expression during germination of maize seeds under water stress |
title_sort |
Heat-resistant protein expression during germination of maize seeds under water stress |
author |
Abreu, V. M. |
author_facet |
Abreu, V. M. Silva Neta, I. C. Von Pinho, Édila Vilela de Resende Naves, G. M. F. Guimarães, R. M. Santos, H. O. Von Pinho, R. G. |
author_role |
author |
author2 |
Silva Neta, I. C. Von Pinho, Édila Vilela de Resende Naves, G. M. F. Guimarães, R. M. Santos, H. O. Von Pinho, R. G. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Abreu, V. M. Silva Neta, I. C. Von Pinho, Édila Vilela de Resende Naves, G. M. F. Guimarães, R. M. Santos, H. O. Von Pinho, R. G. |
dc.subject.por.fl_str_mv |
Zea mays Late embryogenesis Protein abundance Heat shock |
topic |
Zea mays Late embryogenesis Protein abundance Heat shock |
description |
Low water availability is one of the factors that limit agricultural crop development, and hence the development of genotypes with increased water stress tolerance is a challenge in plant breeding programs. Heat-resistant proteins have been widely studied, and are reported to participate in various developmental processes and to accumulate in response to stress. This study aimed to evaluate heat-resistant protein expression under water stress conditions during the germination of maize seed inbreed lines differing in their water stress tolerance. Maize seed lines 91 and 64 were soaked in 0, -0.3, -0.6, and -0.9 MPa water potential for 0, 6, 12, 18, and 24 h. Line 91 is considered more water stress-tolerant than line 64. The analysis of heat-resistant protein expression was made by gel electrophoresis and spectrophotometry. In general, higher expression of heat-resistant proteins was observed in seeds from line 64 subjected to shorter soaking periods and lower water potentials. However, in the water stress-tolerant line 91, a higher expression was observed in seeds that were subjected to -0.3 and -0.6 MPa water potentials. In the absence of water stress, heat-resistant protein expression was reduced with increasing soaking period. Thus, there was a difference in heat-resistant protein expression among the seed lines differing in water stress tolerance. Increased heatresistant protein expression was observed in seeds from line 91 when subjected to water stress conditions for longer soaking periods. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2018-08-29T13:31:35Z 2018-08-29T13:31:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
ABREU, V. M. et al. Heat-resistant protein expression during germination of maize seeds under water stress. Genetics and Molecular Research, Ribeirão Preto, v. 15, n. 3, 2016. http://repositorio.ufla.br/jspui/handle/1/30234 |
identifier_str_mv |
ABREU, V. M. et al. Heat-resistant protein expression during germination of maize seeds under water stress. Genetics and Molecular Research, Ribeirão Preto, v. 15, n. 3, 2016. |
url |
http://repositorio.ufla.br/jspui/handle/1/30234 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution-ShareAlike 4.0 International http://creativecommons.org/licenses/by-sa/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-ShareAlike 4.0 International http://creativecommons.org/licenses/by-sa/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Fundação de Pesquisas Científicas de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação de Pesquisas Científicas de Ribeirão Preto |
dc.source.none.fl_str_mv |
Genetics and Molecular Research reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835052053102592 |