Nuclear DNA content and chromosome number in Brachiaria spp. genotypes

Detalhes bibliográficos
Autor(a) principal: Timbó, Ana Luiza de Oliveira
Data de Publicação: 2014
Outros Autores: Pereira, Roselaine Cristina, Sobrinho, Fausto Souza, Davide, Lisete Chamma
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/39011
Resumo: Breeding programs for Brachiaria spp. use both intraspecies and interspecies crosses between sexual and apomictic plants in order to obtain new cultivars with the desired characteristics. As there are different ploidy levels both within and between species of this genus, it becomes necessary to evaluate the genotypes used in breeding programs, as a guide to breeders when adopting crossing strategies. In this work, DNA content and chromosome number were determined in order to characterise ploidy levels in Brachiaria spp. genotypes. In the analysis of 15 genotypes, DNA content varied with the ploidy levels (2x, 3x and 4x), and between species and/or taxon. The average DNA content was 1.74 pg (2x) in B. ruziziensis, 3.74 pg (4x) in B. decumbens and 3.52 pg (4x) for B. brizantha. For the genotype 86, 2.57 pg of DNA was obtained and 2n = 3x = 27, indicating a triploid accession, probably a natural hybrid. The variation in the total DNA content allowed the differentiation of Brachiaria ruziziensis (2n = 2x = 18) from the tetraploid species Brachiaria Brizantha and Brachiaria decumbens (2n = 4x = 36), as well as the probable hybrid triploid (2n = 3x = 18) of these species.
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spelling Nuclear DNA content and chromosome number in Brachiaria spp. genotypesConteúdo de DNA nuclear e número cromossômico de genótipos de Brachiaria spp.Germplasm bankCytogeneticsFlow cytometryBanco de germoplasmaCitogenéticaCitometria de FluxoForage plantsPlantas forrageirasBreeding programs for Brachiaria spp. use both intraspecies and interspecies crosses between sexual and apomictic plants in order to obtain new cultivars with the desired characteristics. As there are different ploidy levels both within and between species of this genus, it becomes necessary to evaluate the genotypes used in breeding programs, as a guide to breeders when adopting crossing strategies. In this work, DNA content and chromosome number were determined in order to characterise ploidy levels in Brachiaria spp. genotypes. In the analysis of 15 genotypes, DNA content varied with the ploidy levels (2x, 3x and 4x), and between species and/or taxon. The average DNA content was 1.74 pg (2x) in B. ruziziensis, 3.74 pg (4x) in B. decumbens and 3.52 pg (4x) for B. brizantha. For the genotype 86, 2.57 pg of DNA was obtained and 2n = 3x = 27, indicating a triploid accession, probably a natural hybrid. The variation in the total DNA content allowed the differentiation of Brachiaria ruziziensis (2n = 2x = 18) from the tetraploid species Brachiaria Brizantha and Brachiaria decumbens (2n = 4x = 36), as well as the probable hybrid triploid (2n = 3x = 18) of these species.Os programas de melhoramento de Brachiaria spp. utilizam os cruzamentos intraespecíficos e interespecíficos entre plantas sexuais e apomíticas para obtenção de novas cultivares com características desejadas. Como existem diferentes níveis de ploidia dentro e entre as espécies deste gênero, torna-se necessária a avaliação dos genótipos utilizados em programas de melhoramento para a orientação das estratégias de cruzamentos adotadas pelos melhoristas. Neste trabalho, o conteúdo de DNA e o número cromossômico foram determinados para caracterizar o nível de ploidia de genótipos de Brachiaria spp. Na análise de 15 genótipos, o conteúdo de DNA variou de acordo com o nível de ploidia (2x, 3x e 4x) e entre espécie e/ou taxon. O conteúdo de médio de DNA foi de 1,74 pg (2x) para B. ruziziensis, 3,74 pg (4x) para B. decumbens e de 3,52 pg (4x) para B. brizantha. Para o genótipo 86 obteve-se 2,57 pg de DNA e 2n = 3x = 27, indicando tratar-se de um acesso triplóide, provavelmente um híbrido natural. A variação no conteúdo de DNA total permitiu diferenciar Brachiaria ruziziensis (2n = 2x = 18) das espécies tetraploides (2n = 4x = 36) Brachiaria decumbens e Brachiaria brizantha, bem como o provável híbrido triplóide (2n = 3x = 18) dessas espécies.Universidade Federal do Ceará2020-02-12T14:56:48Z2020-02-12T14:56:48Z2014-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfTIMBÓ, A. L. de O.; PEREIRA, R. C.;SOBRINHO, F. S.; DAVIDE, L. C. Nuclear DNA content and chromosome number in Brachiaria spp. Genotypes. Revista Ciência Agronômica, Fortaleza, v. 45, n. 1, p. 62-67, jan./mar. 2014.http://repositorio.ufla.br/jspui/handle/1/39011Revista Ciência Agronômicareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessTimbó, Ana Luiza de OliveiraPereira, Roselaine CristinaSobrinho, Fausto SouzaDavide, Lisete Chammaeng2020-02-12T14:56:48Zoai:localhost:1/39011Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-02-12T14:56:48Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Nuclear DNA content and chromosome number in Brachiaria spp. genotypes
Conteúdo de DNA nuclear e número cromossômico de genótipos de Brachiaria spp.
title Nuclear DNA content and chromosome number in Brachiaria spp. genotypes
spellingShingle Nuclear DNA content and chromosome number in Brachiaria spp. genotypes
Timbó, Ana Luiza de Oliveira
Germplasm bank
Cytogenetics
Flow cytometry
Banco de germoplasma
Citogenética
Citometria de Fluxo
Forage plants
Plantas forrageiras
title_short Nuclear DNA content and chromosome number in Brachiaria spp. genotypes
title_full Nuclear DNA content and chromosome number in Brachiaria spp. genotypes
title_fullStr Nuclear DNA content and chromosome number in Brachiaria spp. genotypes
title_full_unstemmed Nuclear DNA content and chromosome number in Brachiaria spp. genotypes
title_sort Nuclear DNA content and chromosome number in Brachiaria spp. genotypes
author Timbó, Ana Luiza de Oliveira
author_facet Timbó, Ana Luiza de Oliveira
Pereira, Roselaine Cristina
Sobrinho, Fausto Souza
Davide, Lisete Chamma
author_role author
author2 Pereira, Roselaine Cristina
Sobrinho, Fausto Souza
Davide, Lisete Chamma
author2_role author
author
author
dc.contributor.author.fl_str_mv Timbó, Ana Luiza de Oliveira
Pereira, Roselaine Cristina
Sobrinho, Fausto Souza
Davide, Lisete Chamma
dc.subject.por.fl_str_mv Germplasm bank
Cytogenetics
Flow cytometry
Banco de germoplasma
Citogenética
Citometria de Fluxo
Forage plants
Plantas forrageiras
topic Germplasm bank
Cytogenetics
Flow cytometry
Banco de germoplasma
Citogenética
Citometria de Fluxo
Forage plants
Plantas forrageiras
description Breeding programs for Brachiaria spp. use both intraspecies and interspecies crosses between sexual and apomictic plants in order to obtain new cultivars with the desired characteristics. As there are different ploidy levels both within and between species of this genus, it becomes necessary to evaluate the genotypes used in breeding programs, as a guide to breeders when adopting crossing strategies. In this work, DNA content and chromosome number were determined in order to characterise ploidy levels in Brachiaria spp. genotypes. In the analysis of 15 genotypes, DNA content varied with the ploidy levels (2x, 3x and 4x), and between species and/or taxon. The average DNA content was 1.74 pg (2x) in B. ruziziensis, 3.74 pg (4x) in B. decumbens and 3.52 pg (4x) for B. brizantha. For the genotype 86, 2.57 pg of DNA was obtained and 2n = 3x = 27, indicating a triploid accession, probably a natural hybrid. The variation in the total DNA content allowed the differentiation of Brachiaria ruziziensis (2n = 2x = 18) from the tetraploid species Brachiaria Brizantha and Brachiaria decumbens (2n = 4x = 36), as well as the probable hybrid triploid (2n = 3x = 18) of these species.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
2020-02-12T14:56:48Z
2020-02-12T14:56:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv TIMBÓ, A. L. de O.; PEREIRA, R. C.;SOBRINHO, F. S.; DAVIDE, L. C. Nuclear DNA content and chromosome number in Brachiaria spp. Genotypes. Revista Ciência Agronômica, Fortaleza, v. 45, n. 1, p. 62-67, jan./mar. 2014.
http://repositorio.ufla.br/jspui/handle/1/39011
identifier_str_mv TIMBÓ, A. L. de O.; PEREIRA, R. C.;SOBRINHO, F. S.; DAVIDE, L. C. Nuclear DNA content and chromosome number in Brachiaria spp. Genotypes. Revista Ciência Agronômica, Fortaleza, v. 45, n. 1, p. 62-67, jan./mar. 2014.
url http://repositorio.ufla.br/jspui/handle/1/39011
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do Ceará
publisher.none.fl_str_mv Universidade Federal do Ceará
dc.source.none.fl_str_mv Revista Ciência Agronômica
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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