Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/37951 |
Resumo: | Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis. Three hundred fourty four strains of coagulase-positive Staphylococcus (CPS), isolated from mastitis cases, underwent phenotypic and genotypic tests to evaluate the efficiency of a simplified key, based on phenotypic tests for the discrimination of these microorganisms. The tests consisted of amplification of the femA gene and hemolysis in blood agar, production of acetoin and fermentation of maltose, mannitol and trehalose. Strains that showed negative results in the amplification test of the femA gene or that were not identified as Staphylococcus aureus (S. aureus) by phenotypic tests were tested with the APISTAPH kit (Biomériux-France), for precise identification of species. Phenotypic tests revealed 338 strains (98.25%) as S. aureus, three strains (0.86%) as Staphylococcus hyicus, and three microorganisms (0.86%) as Staphylococcus intermedius. PCR demonstrated that 338 (98.25%) strains belonged to the S. aureus species, confirming the results for 336 strains from 338 identified, through a simplified phenotypic key. A high rate of correlation (98.83%) was verifeid between the results of genotypic and phenotypic tests for the identification of S. aureus, demonstrating the applicability of the proposed key, for the discrimination of this microorganism in CPS isolated from bovine mastitis. |
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Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitisAvaliação de uma chave de identificação simplificada para Staphylococcuscoagulase-positivos isolados de mastite bovinaMastitis pathogensCoagulase testBiochemical testsPolymerase Chain Reaction (PCR)Patógenos da mastiteTeste de coagulasetestes bioquímicosEvaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis. Three hundred fourty four strains of coagulase-positive Staphylococcus (CPS), isolated from mastitis cases, underwent phenotypic and genotypic tests to evaluate the efficiency of a simplified key, based on phenotypic tests for the discrimination of these microorganisms. The tests consisted of amplification of the femA gene and hemolysis in blood agar, production of acetoin and fermentation of maltose, mannitol and trehalose. Strains that showed negative results in the amplification test of the femA gene or that were not identified as Staphylococcus aureus (S. aureus) by phenotypic tests were tested with the APISTAPH kit (Biomériux-France), for precise identification of species. Phenotypic tests revealed 338 strains (98.25%) as S. aureus, three strains (0.86%) as Staphylococcus hyicus, and three microorganisms (0.86%) as Staphylococcus intermedius. PCR demonstrated that 338 (98.25%) strains belonged to the S. aureus species, confirming the results for 336 strains from 338 identified, through a simplified phenotypic key. A high rate of correlation (98.83%) was verifeid between the results of genotypic and phenotypic tests for the identification of S. aureus, demonstrating the applicability of the proposed key, for the discrimination of this microorganism in CPS isolated from bovine mastitis.Visando testar a eficiência de uma chave simplificada baseada em testes fenotípicos para a discriminação de Staphylocococcus coagulase positivos (SCP) isolados de infecções intramamárias de bovinos, 344 amostras destes microrganismos foram submetidas a testes fenotípicos e genotípicos. Estes consistiram na amplificação do gene femA, na observação de hemólise em ágar sangue, produção de acetoína e fermentação de maltose, manitol e trealose. Amostras que apresentaram resultado negativo na amplificação do gene femA ou que foram identificadas com não Staphylococcus aureus (S. aureus) por meio dos testes fenotípicos foram submetidas ao kit APISTAPH (Biomériux-França) para identificação mais precisa. Os testes fenotípicos utilizados na chave simplificada permitiram identificar 338 amostras (98,25%) como S. aureus, três amostras (0,86%) como Staphylococcus hyicus e três (0,86%) como Staphylococcus intermedius. Por meio da reação em cadeia da polimerase (PCR) 338 (98,25%) amostras foram identificadas como S. aureus, ratificando os resultados para 336 das 338 amostras identificadas por meio da chave fenotípica simplificada. Observou-se elevada concordância (98,83%) entre os resultados dos testes genotípicos e fenotípicos para a identificação de S. aureus, demonstrando a aplicabilidade da chave de identificação proposta para a discriminação deste microrganismo entre SCP isolados de casos de mastite bovina.Universidade Estadual de Maringá2019-11-30T13:46:32Z2019-11-30T13:46:32Z2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCOSTA, G. M. da et al. Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis. Acta Scientiarum. Biological Sciences, Maringá, v. 32, n. 4, p. 403-406, 2010.http://repositorio.ufla.br/jspui/handle/1/37951Acta Scientiarum. Biological Sciencesreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessCosta, Geraldo Márcio daPaiva, Luciano VilelaPiccoli, Roberta HilsdorfFigueiredo, Demétrio JunqueiraPereira, Ulisses de PáduaSilva, Nivaldo daeng2019-11-30T13:46:55Zoai:localhost:1/37951Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-11-30T13:46:55Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis Avaliação de uma chave de identificação simplificada para Staphylococcuscoagulase-positivos isolados de mastite bovina |
title |
Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis |
spellingShingle |
Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis Costa, Geraldo Márcio da Mastitis pathogens Coagulase test Biochemical tests Polymerase Chain Reaction (PCR) Patógenos da mastite Teste de coagulase testes bioquímicos |
title_short |
Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis |
title_full |
Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis |
title_fullStr |
Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis |
title_full_unstemmed |
Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis |
title_sort |
Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis |
author |
Costa, Geraldo Márcio da |
author_facet |
Costa, Geraldo Márcio da Paiva, Luciano Vilela Piccoli, Roberta Hilsdorf Figueiredo, Demétrio Junqueira Pereira, Ulisses de Pádua Silva, Nivaldo da |
author_role |
author |
author2 |
Paiva, Luciano Vilela Piccoli, Roberta Hilsdorf Figueiredo, Demétrio Junqueira Pereira, Ulisses de Pádua Silva, Nivaldo da |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Costa, Geraldo Márcio da Paiva, Luciano Vilela Piccoli, Roberta Hilsdorf Figueiredo, Demétrio Junqueira Pereira, Ulisses de Pádua Silva, Nivaldo da |
dc.subject.por.fl_str_mv |
Mastitis pathogens Coagulase test Biochemical tests Polymerase Chain Reaction (PCR) Patógenos da mastite Teste de coagulase testes bioquímicos |
topic |
Mastitis pathogens Coagulase test Biochemical tests Polymerase Chain Reaction (PCR) Patógenos da mastite Teste de coagulase testes bioquímicos |
description |
Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis. Three hundred fourty four strains of coagulase-positive Staphylococcus (CPS), isolated from mastitis cases, underwent phenotypic and genotypic tests to evaluate the efficiency of a simplified key, based on phenotypic tests for the discrimination of these microorganisms. The tests consisted of amplification of the femA gene and hemolysis in blood agar, production of acetoin and fermentation of maltose, mannitol and trehalose. Strains that showed negative results in the amplification test of the femA gene or that were not identified as Staphylococcus aureus (S. aureus) by phenotypic tests were tested with the APISTAPH kit (Biomériux-France), for precise identification of species. Phenotypic tests revealed 338 strains (98.25%) as S. aureus, three strains (0.86%) as Staphylococcus hyicus, and three microorganisms (0.86%) as Staphylococcus intermedius. PCR demonstrated that 338 (98.25%) strains belonged to the S. aureus species, confirming the results for 336 strains from 338 identified, through a simplified phenotypic key. A high rate of correlation (98.83%) was verifeid between the results of genotypic and phenotypic tests for the identification of S. aureus, demonstrating the applicability of the proposed key, for the discrimination of this microorganism in CPS isolated from bovine mastitis. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2019-11-30T13:46:32Z 2019-11-30T13:46:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
COSTA, G. M. da et al. Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis. Acta Scientiarum. Biological Sciences, Maringá, v. 32, n. 4, p. 403-406, 2010. http://repositorio.ufla.br/jspui/handle/1/37951 |
identifier_str_mv |
COSTA, G. M. da et al. Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis. Acta Scientiarum. Biological Sciences, Maringá, v. 32, n. 4, p. 403-406, 2010. |
url |
http://repositorio.ufla.br/jspui/handle/1/37951 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1807835220097892352 |