Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil

Detalhes bibliográficos
Autor(a) principal: Andrade, Rafaella Silva
Data de Publicação: 2023
Outros Autores: Pereira, Carine Rodrigues, Soares Filho, Paulo Martins, Souza, Patrícia Gomes de, Fonseca Júnior, Antônio Augusto, Minharro, Silvia, Costa, Marisa da, Dorneles, Elaine Maria Seles, Lage, Andrey Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/56531
Resumo: The aim of the present study was to characterize (phenotypically and genotypically) two strains of Brucella abortus identified as belonging to biovar 4 isolated from cattle in Brazil. The strains were isolated from cervical bursitis from cattle in the states of Pará and Rio Grande do Sul, respectively. In the phenotypic identification, the isolates were positive in CO2 requirement, produced H2S, were resistant to basic fuchsin (20 µg / mL) and sensitive to thionin (20 µg / mL and 40 µg / mL) and presented M surface antigen, but A surface antigen is absent. The isolates were positive in the PCR for the bcsp31 gene (genus-specific) and in the AMOS-enhanced PCR, both isolates showed a band profile consistent with B. abortus biovar 1, 2 or 4. Moreover, both isolates also showed restriction patterns identical to the reference strain when tested by the omp2b PCR-RFLP. In genotyping using Multiple Locus Variable Number of Tandem Repeat (VNTR) Analysis - MLVA (MLVA16), the isolates showed differences in several loci (Bruce42, Bruce19, Bruce04, Bruce16 and Bruce30); by Multiple Locus Sequence Typing (MLST), they also exhibited differences in sequence type (ST), strain 16/02 ST1 (2-1-1-2-1-3-1-1-1) and strain 128/11 ST (22-1-1 -8-9-3-1-1-1). The extensive typing of B. abortus strains isolated from cattle in Brazil using different approaches confirmed the occurrence of rare B. abortus biovar 4 in the country.
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spelling Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in BrazilCaracterização fenotípica e genotípica de isolados de Brucella abortus biovar 4 em bovinos no BrasilBrucellosisGenotypingMultiple-locus variable number tandem repeat analysis (MLVA)Multiple Locus Sequence Typing (MLST)BruceloseGenotipagemThe aim of the present study was to characterize (phenotypically and genotypically) two strains of Brucella abortus identified as belonging to biovar 4 isolated from cattle in Brazil. The strains were isolated from cervical bursitis from cattle in the states of Pará and Rio Grande do Sul, respectively. In the phenotypic identification, the isolates were positive in CO2 requirement, produced H2S, were resistant to basic fuchsin (20 µg / mL) and sensitive to thionin (20 µg / mL and 40 µg / mL) and presented M surface antigen, but A surface antigen is absent. The isolates were positive in the PCR for the bcsp31 gene (genus-specific) and in the AMOS-enhanced PCR, both isolates showed a band profile consistent with B. abortus biovar 1, 2 or 4. Moreover, both isolates also showed restriction patterns identical to the reference strain when tested by the omp2b PCR-RFLP. In genotyping using Multiple Locus Variable Number of Tandem Repeat (VNTR) Analysis - MLVA (MLVA16), the isolates showed differences in several loci (Bruce42, Bruce19, Bruce04, Bruce16 and Bruce30); by Multiple Locus Sequence Typing (MLST), they also exhibited differences in sequence type (ST), strain 16/02 ST1 (2-1-1-2-1-3-1-1-1) and strain 128/11 ST (22-1-1 -8-9-3-1-1-1). The extensive typing of B. abortus strains isolated from cattle in Brazil using different approaches confirmed the occurrence of rare B. abortus biovar 4 in the country.O objetivo do presente estudo foi caracterizar (fenotipicamente e genotipicamente) duas cepas de Brucella abortus identificadas como pertencentes ao biovar 4 isolada de bovinos no Brasil. As cepas foram isoladas de bursite cervical de bovinos dos estados do Pará e Rio Grande do Sul, respectivamente. Na identificação fenotípica, os isolados foram positivos na exigência de CO2 , produziram H2 S, foram resistentes à fucsina básica (20 µg / mL) e sensíveis à tionina (20 µg / mL e 40 µg / mL) e apresentaram antígeno de superfície M, mas o antígeno de superfície A foi ausente. Os isolados foram positivos na PCR para o gene bcsp31 (gênero específico) e na PCR - AMOS, ambos os isolados apresentaram perfil de banda consistente com B. abortus biovar 1, 2 ou 4. Além disso, ambos os isolados também apresentaram padrões de restrição idêntica à cepa de referência quando testada pelo omp2b PCR-RFLP. Na genotipagem usando Multiple Locus Variable Number of Tandem Repeat (VNTR) - MLVA (MLVA16), os isolados apresentaram diferenças em vários loci (Bruce42, Bruce19, Bruce04, Bruce16 e Bruce30); no Multiple Locus Sequence Typing (MLST), os isolados também exibiram diferenças na sequência tipo (ST), amostra 16/02 ST1 (2-1-1-2-1-3-1-1-1) e amostra 128/11 ST (22-1-1-8-9-3-1-1-1). A extensa tipagem de cepas de B. abortus isoladas de bovinos no Brasil por diferentes abordagens confirmou a rara ocorrência de B. abortus biovar 4 no país.Universidade Federal de Santa Maria2023-04-10T18:46:18Z2023-04-10T18:46:18Z2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfANDRADE, R. S. et al. Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil. Ciência Rural, Santa Maria, v. 53, n. 6, e20210564, 2023. DOI: https://doi.org/10.1590/0103-8478cr20210564.http://repositorio.ufla.br/jspui/handle/1/56531Ciência Ruralreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessAndrade, Rafaella SilvaPereira, Carine RodriguesSoares Filho, Paulo MartinsSouza, Patrícia Gomes deFonseca Júnior, Antônio AugustoMinharro, SilviaCosta, Marisa daDorneles, Elaine Maria SelesLage, Andrey Pereiraeng2023-04-10T18:58:42Zoai:localhost:1/56531Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-04-10T18:58:42Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil
Caracterização fenotípica e genotípica de isolados de Brucella abortus biovar 4 em bovinos no Brasil
title Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil
spellingShingle Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil
Andrade, Rafaella Silva
Brucellosis
Genotyping
Multiple-locus variable number tandem repeat analysis (MLVA)
Multiple Locus Sequence Typing (MLST)
Brucelose
Genotipagem
title_short Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil
title_full Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil
title_fullStr Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil
title_full_unstemmed Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil
title_sort Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil
author Andrade, Rafaella Silva
author_facet Andrade, Rafaella Silva
Pereira, Carine Rodrigues
Soares Filho, Paulo Martins
Souza, Patrícia Gomes de
Fonseca Júnior, Antônio Augusto
Minharro, Silvia
Costa, Marisa da
Dorneles, Elaine Maria Seles
Lage, Andrey Pereira
author_role author
author2 Pereira, Carine Rodrigues
Soares Filho, Paulo Martins
Souza, Patrícia Gomes de
Fonseca Júnior, Antônio Augusto
Minharro, Silvia
Costa, Marisa da
Dorneles, Elaine Maria Seles
Lage, Andrey Pereira
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Andrade, Rafaella Silva
Pereira, Carine Rodrigues
Soares Filho, Paulo Martins
Souza, Patrícia Gomes de
Fonseca Júnior, Antônio Augusto
Minharro, Silvia
Costa, Marisa da
Dorneles, Elaine Maria Seles
Lage, Andrey Pereira
dc.subject.por.fl_str_mv Brucellosis
Genotyping
Multiple-locus variable number tandem repeat analysis (MLVA)
Multiple Locus Sequence Typing (MLST)
Brucelose
Genotipagem
topic Brucellosis
Genotyping
Multiple-locus variable number tandem repeat analysis (MLVA)
Multiple Locus Sequence Typing (MLST)
Brucelose
Genotipagem
description The aim of the present study was to characterize (phenotypically and genotypically) two strains of Brucella abortus identified as belonging to biovar 4 isolated from cattle in Brazil. The strains were isolated from cervical bursitis from cattle in the states of Pará and Rio Grande do Sul, respectively. In the phenotypic identification, the isolates were positive in CO2 requirement, produced H2S, were resistant to basic fuchsin (20 µg / mL) and sensitive to thionin (20 µg / mL and 40 µg / mL) and presented M surface antigen, but A surface antigen is absent. The isolates were positive in the PCR for the bcsp31 gene (genus-specific) and in the AMOS-enhanced PCR, both isolates showed a band profile consistent with B. abortus biovar 1, 2 or 4. Moreover, both isolates also showed restriction patterns identical to the reference strain when tested by the omp2b PCR-RFLP. In genotyping using Multiple Locus Variable Number of Tandem Repeat (VNTR) Analysis - MLVA (MLVA16), the isolates showed differences in several loci (Bruce42, Bruce19, Bruce04, Bruce16 and Bruce30); by Multiple Locus Sequence Typing (MLST), they also exhibited differences in sequence type (ST), strain 16/02 ST1 (2-1-1-2-1-3-1-1-1) and strain 128/11 ST (22-1-1 -8-9-3-1-1-1). The extensive typing of B. abortus strains isolated from cattle in Brazil using different approaches confirmed the occurrence of rare B. abortus biovar 4 in the country.
publishDate 2023
dc.date.none.fl_str_mv 2023-04-10T18:46:18Z
2023-04-10T18:46:18Z
2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv ANDRADE, R. S. et al. Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil. Ciência Rural, Santa Maria, v. 53, n. 6, e20210564, 2023. DOI: https://doi.org/10.1590/0103-8478cr20210564.
http://repositorio.ufla.br/jspui/handle/1/56531
identifier_str_mv ANDRADE, R. S. et al. Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil. Ciência Rural, Santa Maria, v. 53, n. 6, e20210564, 2023. DOI: https://doi.org/10.1590/0103-8478cr20210564.
url http://repositorio.ufla.br/jspui/handle/1/56531
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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