Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/56531 |
Resumo: | The aim of the present study was to characterize (phenotypically and genotypically) two strains of Brucella abortus identified as belonging to biovar 4 isolated from cattle in Brazil. The strains were isolated from cervical bursitis from cattle in the states of Pará and Rio Grande do Sul, respectively. In the phenotypic identification, the isolates were positive in CO2 requirement, produced H2S, were resistant to basic fuchsin (20 µg / mL) and sensitive to thionin (20 µg / mL and 40 µg / mL) and presented M surface antigen, but A surface antigen is absent. The isolates were positive in the PCR for the bcsp31 gene (genus-specific) and in the AMOS-enhanced PCR, both isolates showed a band profile consistent with B. abortus biovar 1, 2 or 4. Moreover, both isolates also showed restriction patterns identical to the reference strain when tested by the omp2b PCR-RFLP. In genotyping using Multiple Locus Variable Number of Tandem Repeat (VNTR) Analysis - MLVA (MLVA16), the isolates showed differences in several loci (Bruce42, Bruce19, Bruce04, Bruce16 and Bruce30); by Multiple Locus Sequence Typing (MLST), they also exhibited differences in sequence type (ST), strain 16/02 ST1 (2-1-1-2-1-3-1-1-1) and strain 128/11 ST (22-1-1 -8-9-3-1-1-1). The extensive typing of B. abortus strains isolated from cattle in Brazil using different approaches confirmed the occurrence of rare B. abortus biovar 4 in the country. |
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Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in BrazilCaracterização fenotípica e genotípica de isolados de Brucella abortus biovar 4 em bovinos no BrasilBrucellosisGenotypingMultiple-locus variable number tandem repeat analysis (MLVA)Multiple Locus Sequence Typing (MLST)BruceloseGenotipagemThe aim of the present study was to characterize (phenotypically and genotypically) two strains of Brucella abortus identified as belonging to biovar 4 isolated from cattle in Brazil. The strains were isolated from cervical bursitis from cattle in the states of Pará and Rio Grande do Sul, respectively. In the phenotypic identification, the isolates were positive in CO2 requirement, produced H2S, were resistant to basic fuchsin (20 µg / mL) and sensitive to thionin (20 µg / mL and 40 µg / mL) and presented M surface antigen, but A surface antigen is absent. The isolates were positive in the PCR for the bcsp31 gene (genus-specific) and in the AMOS-enhanced PCR, both isolates showed a band profile consistent with B. abortus biovar 1, 2 or 4. Moreover, both isolates also showed restriction patterns identical to the reference strain when tested by the omp2b PCR-RFLP. In genotyping using Multiple Locus Variable Number of Tandem Repeat (VNTR) Analysis - MLVA (MLVA16), the isolates showed differences in several loci (Bruce42, Bruce19, Bruce04, Bruce16 and Bruce30); by Multiple Locus Sequence Typing (MLST), they also exhibited differences in sequence type (ST), strain 16/02 ST1 (2-1-1-2-1-3-1-1-1) and strain 128/11 ST (22-1-1 -8-9-3-1-1-1). The extensive typing of B. abortus strains isolated from cattle in Brazil using different approaches confirmed the occurrence of rare B. abortus biovar 4 in the country.O objetivo do presente estudo foi caracterizar (fenotipicamente e genotipicamente) duas cepas de Brucella abortus identificadas como pertencentes ao biovar 4 isolada de bovinos no Brasil. As cepas foram isoladas de bursite cervical de bovinos dos estados do Pará e Rio Grande do Sul, respectivamente. Na identificação fenotípica, os isolados foram positivos na exigência de CO2 , produziram H2 S, foram resistentes à fucsina básica (20 µg / mL) e sensíveis à tionina (20 µg / mL e 40 µg / mL) e apresentaram antígeno de superfície M, mas o antígeno de superfície A foi ausente. Os isolados foram positivos na PCR para o gene bcsp31 (gênero específico) e na PCR - AMOS, ambos os isolados apresentaram perfil de banda consistente com B. abortus biovar 1, 2 ou 4. Além disso, ambos os isolados também apresentaram padrões de restrição idêntica à cepa de referência quando testada pelo omp2b PCR-RFLP. Na genotipagem usando Multiple Locus Variable Number of Tandem Repeat (VNTR) - MLVA (MLVA16), os isolados apresentaram diferenças em vários loci (Bruce42, Bruce19, Bruce04, Bruce16 e Bruce30); no Multiple Locus Sequence Typing (MLST), os isolados também exibiram diferenças na sequência tipo (ST), amostra 16/02 ST1 (2-1-1-2-1-3-1-1-1) e amostra 128/11 ST (22-1-1-8-9-3-1-1-1). A extensa tipagem de cepas de B. abortus isoladas de bovinos no Brasil por diferentes abordagens confirmou a rara ocorrência de B. abortus biovar 4 no país.Universidade Federal de Santa Maria2023-04-10T18:46:18Z2023-04-10T18:46:18Z2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfANDRADE, R. S. et al. Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil. Ciência Rural, Santa Maria, v. 53, n. 6, e20210564, 2023. DOI: https://doi.org/10.1590/0103-8478cr20210564.http://repositorio.ufla.br/jspui/handle/1/56531Ciência Ruralreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessAndrade, Rafaella SilvaPereira, Carine RodriguesSoares Filho, Paulo MartinsSouza, Patrícia Gomes deFonseca Júnior, Antônio AugustoMinharro, SilviaCosta, Marisa daDorneles, Elaine Maria SelesLage, Andrey Pereiraeng2023-04-10T18:58:42Zoai:localhost:1/56531Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-04-10T18:58:42Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil Caracterização fenotípica e genotípica de isolados de Brucella abortus biovar 4 em bovinos no Brasil |
title |
Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil |
spellingShingle |
Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil Andrade, Rafaella Silva Brucellosis Genotyping Multiple-locus variable number tandem repeat analysis (MLVA) Multiple Locus Sequence Typing (MLST) Brucelose Genotipagem |
title_short |
Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil |
title_full |
Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil |
title_fullStr |
Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil |
title_full_unstemmed |
Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil |
title_sort |
Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil |
author |
Andrade, Rafaella Silva |
author_facet |
Andrade, Rafaella Silva Pereira, Carine Rodrigues Soares Filho, Paulo Martins Souza, Patrícia Gomes de Fonseca Júnior, Antônio Augusto Minharro, Silvia Costa, Marisa da Dorneles, Elaine Maria Seles Lage, Andrey Pereira |
author_role |
author |
author2 |
Pereira, Carine Rodrigues Soares Filho, Paulo Martins Souza, Patrícia Gomes de Fonseca Júnior, Antônio Augusto Minharro, Silvia Costa, Marisa da Dorneles, Elaine Maria Seles Lage, Andrey Pereira |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Andrade, Rafaella Silva Pereira, Carine Rodrigues Soares Filho, Paulo Martins Souza, Patrícia Gomes de Fonseca Júnior, Antônio Augusto Minharro, Silvia Costa, Marisa da Dorneles, Elaine Maria Seles Lage, Andrey Pereira |
dc.subject.por.fl_str_mv |
Brucellosis Genotyping Multiple-locus variable number tandem repeat analysis (MLVA) Multiple Locus Sequence Typing (MLST) Brucelose Genotipagem |
topic |
Brucellosis Genotyping Multiple-locus variable number tandem repeat analysis (MLVA) Multiple Locus Sequence Typing (MLST) Brucelose Genotipagem |
description |
The aim of the present study was to characterize (phenotypically and genotypically) two strains of Brucella abortus identified as belonging to biovar 4 isolated from cattle in Brazil. The strains were isolated from cervical bursitis from cattle in the states of Pará and Rio Grande do Sul, respectively. In the phenotypic identification, the isolates were positive in CO2 requirement, produced H2S, were resistant to basic fuchsin (20 µg / mL) and sensitive to thionin (20 µg / mL and 40 µg / mL) and presented M surface antigen, but A surface antigen is absent. The isolates were positive in the PCR for the bcsp31 gene (genus-specific) and in the AMOS-enhanced PCR, both isolates showed a band profile consistent with B. abortus biovar 1, 2 or 4. Moreover, both isolates also showed restriction patterns identical to the reference strain when tested by the omp2b PCR-RFLP. In genotyping using Multiple Locus Variable Number of Tandem Repeat (VNTR) Analysis - MLVA (MLVA16), the isolates showed differences in several loci (Bruce42, Bruce19, Bruce04, Bruce16 and Bruce30); by Multiple Locus Sequence Typing (MLST), they also exhibited differences in sequence type (ST), strain 16/02 ST1 (2-1-1-2-1-3-1-1-1) and strain 128/11 ST (22-1-1 -8-9-3-1-1-1). The extensive typing of B. abortus strains isolated from cattle in Brazil using different approaches confirmed the occurrence of rare B. abortus biovar 4 in the country. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-04-10T18:46:18Z 2023-04-10T18:46:18Z 2023 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
ANDRADE, R. S. et al. Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil. Ciência Rural, Santa Maria, v. 53, n. 6, e20210564, 2023. DOI: https://doi.org/10.1590/0103-8478cr20210564. http://repositorio.ufla.br/jspui/handle/1/56531 |
identifier_str_mv |
ANDRADE, R. S. et al. Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil. Ciência Rural, Santa Maria, v. 53, n. 6, e20210564, 2023. DOI: https://doi.org/10.1590/0103-8478cr20210564. |
url |
http://repositorio.ufla.br/jspui/handle/1/56531 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1815439208016248832 |