Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides

Detalhes bibliográficos
Autor(a) principal: Maia, Fernanda Gonçalves Martins
Data de Publicação: 2012
Outros Autores: Ogoshi, Claudio, Pierre, Rosana Oliveira, Vieira, Jucilayne Fernandes, Ribeiro junior, Pedro Martins, Abreu, Mario Sobral
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/248
http://repositorio.ufla.br/jspui/handle/1/13669
Resumo: The objective of this work was to study the effects of different isolates of Colletotrichum gloeosporioides, as topigment level (chlorophyll a, b and total), total soluble phenols and soluble lignin in coffee seedlings obtained by artificially inoculatedtissue culture. The experiment was carried out in randomized block design in a 2 x 3 factorial scheme (2 genetic materials), 3 isolates(an isolate from mango fruits and another one from coffee plants), with 5 replicates and control (plants without inoculation). Theplants were inoculated with a suspension of 2 x 106 conidia.ml-1. The conidial suspension was sprayed on previously injured leaves.Leaves of inoculated plants were collected at 3 and 7 days after inoculation, except for leaves on the lower part of the plants.Afterwards, samples were prepared for the evaluation of chlorophyll, total soluble phenols and soluble lignin. The results showedthat the presence of the pathogen in healthy and diseased plants significantly reduced the amount of chlorophyll. At 7 days afterinoculation of healthy and diseased coffee plants higher total soluble phenol and soluble lignin levels were observed in response to theattack by Colletotrichum gloeosporioides.
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spelling Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioidesPigmentos, fenóis solúveis totais e lignina em plântulas de cafeeiro inoculadas com Colletotrichum gloeosporioidesScences AgriculturalCoffea arabicaCiências agráriasCoffea arabicaResponse defenseResposta de defesaColletotrichum gloeosporioidesThe objective of this work was to study the effects of different isolates of Colletotrichum gloeosporioides, as topigment level (chlorophyll a, b and total), total soluble phenols and soluble lignin in coffee seedlings obtained by artificially inoculatedtissue culture. The experiment was carried out in randomized block design in a 2 x 3 factorial scheme (2 genetic materials), 3 isolates(an isolate from mango fruits and another one from coffee plants), with 5 replicates and control (plants without inoculation). Theplants were inoculated with a suspension of 2 x 106 conidia.ml-1. The conidial suspension was sprayed on previously injured leaves.Leaves of inoculated plants were collected at 3 and 7 days after inoculation, except for leaves on the lower part of the plants.Afterwards, samples were prepared for the evaluation of chlorophyll, total soluble phenols and soluble lignin. The results showedthat the presence of the pathogen in healthy and diseased plants significantly reduced the amount of chlorophyll. At 7 days afterinoculation of healthy and diseased coffee plants higher total soluble phenol and soluble lignin levels were observed in response to theattack by Colletotrichum gloeosporioides.Objetivou-se, neste trabalho, estudar os efeitos de diferentes isolados de Colletotrichum gloeosporioides sobre osteores de pigmentos (clorofila a, b e total), fenóis solúveis totais e lignina solúvel, em dois diferentes tempos, sobre mudas de caféobtidas por cultura de tecidos e inoculadas artificialmente. O experimento foi conduzido em blocos casualizados em esquemafatorial 2 x 3 (2 materiais génticos), 3 isolados (um obtido de mangueira e 2 isolados obtidos de plantas de café) + 2 (testemunhassem inoculação), com 5 repetições. As plantas foram inoculadas com uma suspensão de 2 x 106 conídios.ml-1. A suspensão deconídios foi pulverizada sobre folhas de cafeeiro, previamente feridas. Foram realizadas duas coletas, aos 3 e 7 dias apósinoculação, de todas as folhas da muda, exceto as folhas baixeiras. Em seguida, foi feito o preparo das amostras para avaliaçãode clorofila, fenóis solúveis totais e lignina solúvel. A partir dos resultados, pode-se concluir que a presença do patógeno, tantoem plantas sadias, como em plantas doentes diminuíram significativamente a quantidade de clorofila. Aos 7 dias após inoculaçãodas plantas de café sadias e doentes, observou-se um maior teor de fenóis solúveis totais e lignina solúvel em resposta ao ataquede Colletotrichum gloeosporioides.2012-08-062017-08-01T20:06:53Z2017-08-01T20:06:53Z2017-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/248MAIA, F. G. M. et al. Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides. Coffee Science, Lavras, v. 7, n. 2, p. 152-159, maio/ago. 2012.http://repositorio.ufla.br/jspui/handle/1/13669Coffee Science; v. 7, n. 2 (2012); 152-159Coffee Science; v. 7, n. 2 (2012); 152-159Coffee Science; v. 7, n. 2 (2012); 152-1591984-39091809-6875reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAenghttp://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/248/pdfAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessMaia, Fernanda Gonçalves MartinsOgoshi, ClaudioPierre, Rosana OliveiraVieira, Jucilayne FernandesRibeiro junior, Pedro MartinsAbreu, Mario SobralMaia, Fernanda Gonçalves MartinsOgoshi, ClaudioPierre, Rosana OliveiraVieira, Jucilayne FernandesRibeiro junior, Pedro MartinsAbreu, Mario Sobral2021-01-08T16:45:46Zoai:localhost:1/13669Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2021-01-08T16:45:46Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides
Pigmentos, fenóis solúveis totais e lignina em plântulas de cafeeiro inoculadas com Colletotrichum gloeosporioides
title Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides
spellingShingle Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides
Maia, Fernanda Gonçalves Martins
Scences Agricultural
Coffea arabica
Ciências agrárias
Coffea arabica
Response defense
Resposta de defesa
Colletotrichum gloeosporioides
title_short Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides
title_full Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides
title_fullStr Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides
title_full_unstemmed Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides
title_sort Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides
author Maia, Fernanda Gonçalves Martins
author_facet Maia, Fernanda Gonçalves Martins
Ogoshi, Claudio
Pierre, Rosana Oliveira
Vieira, Jucilayne Fernandes
Ribeiro junior, Pedro Martins
Abreu, Mario Sobral
author_role author
author2 Ogoshi, Claudio
Pierre, Rosana Oliveira
Vieira, Jucilayne Fernandes
Ribeiro junior, Pedro Martins
Abreu, Mario Sobral
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Maia, Fernanda Gonçalves Martins
Ogoshi, Claudio
Pierre, Rosana Oliveira
Vieira, Jucilayne Fernandes
Ribeiro junior, Pedro Martins
Abreu, Mario Sobral
Maia, Fernanda Gonçalves Martins
Ogoshi, Claudio
Pierre, Rosana Oliveira
Vieira, Jucilayne Fernandes
Ribeiro junior, Pedro Martins
Abreu, Mario Sobral
dc.subject.por.fl_str_mv Scences Agricultural
Coffea arabica
Ciências agrárias
Coffea arabica
Response defense
Resposta de defesa
Colletotrichum gloeosporioides
topic Scences Agricultural
Coffea arabica
Ciências agrárias
Coffea arabica
Response defense
Resposta de defesa
Colletotrichum gloeosporioides
description The objective of this work was to study the effects of different isolates of Colletotrichum gloeosporioides, as topigment level (chlorophyll a, b and total), total soluble phenols and soluble lignin in coffee seedlings obtained by artificially inoculatedtissue culture. The experiment was carried out in randomized block design in a 2 x 3 factorial scheme (2 genetic materials), 3 isolates(an isolate from mango fruits and another one from coffee plants), with 5 replicates and control (plants without inoculation). Theplants were inoculated with a suspension of 2 x 106 conidia.ml-1. The conidial suspension was sprayed on previously injured leaves.Leaves of inoculated plants were collected at 3 and 7 days after inoculation, except for leaves on the lower part of the plants.Afterwards, samples were prepared for the evaluation of chlorophyll, total soluble phenols and soluble lignin. The results showedthat the presence of the pathogen in healthy and diseased plants significantly reduced the amount of chlorophyll. At 7 days afterinoculation of healthy and diseased coffee plants higher total soluble phenol and soluble lignin levels were observed in response to theattack by Colletotrichum gloeosporioides.
publishDate 2012
dc.date.none.fl_str_mv 2012-08-06
2017-08-01T20:06:53Z
2017-08-01T20:06:53Z
2017-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/248
MAIA, F. G. M. et al. Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides. Coffee Science, Lavras, v. 7, n. 2, p. 152-159, maio/ago. 2012.
http://repositorio.ufla.br/jspui/handle/1/13669
url http://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/248
http://repositorio.ufla.br/jspui/handle/1/13669
identifier_str_mv MAIA, F. G. M. et al. Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides. Coffee Science, Lavras, v. 7, n. 2, p. 152-159, maio/ago. 2012.
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/248/pdf
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.source.none.fl_str_mv Coffee Science; v. 7, n. 2 (2012); 152-159
Coffee Science; v. 7, n. 2 (2012); 152-159
Coffee Science; v. 7, n. 2 (2012); 152-159
1984-3909
1809-6875
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1815438981284757504

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