Multiplicação in vitro de amoreira-preta cultivar Brazos

• Autor(a) principal: Villa, Fabiola
• Data de Publicação: 2006
• Outros Autores: Fráguas, Chrystiane Borges, Dutra, Leonardo Ferreira, Pio, Leila Aparecida Salles, Pasqual, Moacir
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UFLA
• Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000200011; http://repositorio.ufla.br/jspui/handle/1/6196
• topic: Rubus idaeus L.; Micropropagação; Benzilaminopurina; Meio de cultura WPM; Micropropagation; WPM culture medium

With the objective of multiplying blackberry cv. Brazos, nodal segments, coming from in vitro plants previously selected, were excised and inoculated in WPM culture medium (0, 50, 100, 150 and 200%), supplemented with different concentrations of BAP (0; 0,5; 1,0; 2,0 and 4,0 mg L-1). After inoculation, the explants were transferred to culture room, at 27±1ºC temperature, 35 mmol m2 s1 ofirradiance and photoperiod of 16 hours, for 60 days. The experimental was a design randomized complete block, with four replications and four explants each. Greater number of sprouts was provided with 1,0 mg L-1 of BAP associated with 100% WPM culture medium and larger sprouts length average after 60 days were verified in 1,0 mg L-1 of BAP associated with 200% WPM culture medium. Higher dry matter weight of the aerial part was obtained in 200% WPM culture medium added with 0,5 mg L-1 of BAP.