Multiplicação in vitro do porta-enxerto de videira 'VR043-43' (Vitis vinifera x Vitis rotundifolia)
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000400009 http://repositorio.ufla.br/jspui/handle/1/6244 |
Resumo: | The micropropagation is one technique that makes possible the massal propagation of plants of economic interest. The objective of this work was to evaluate the effect of 6–benzilaminopurina (BAP) and kinetin (KIN) in the multiplication in vitro of the grapevine rootstock 'VR043–43'. The isolation was carried through in culture medium QL, supplemented with BAP or KIN, in the concentrations of 0; 2,5; 5,0 and 10,0µM. The subcultures had been carried out each 45 days. The number of shoots per explant increased along with the subcultures with BAP. However this effect was not observed with the KIN that it got resulted similar to the one of the cytokin–free (1,0 shoot/explant). The concentrations of 5,0 nd 10,0 µM of BAP had promoted the biggest number of shoots with reduction of the height. The highest concentration of KIN (10,0 µM) also had negative effect in the height of the shoots, as well as, in the number of leaves per shoot. The callus formation (100%) was observed with BAP and KIN in all the concentrations, and in the cytokinin absence it did not have the callus formation. Cytokinin BAP reduced the formation of roots that was of 100% with the KIN and cytokinin–free. |
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Multiplicação in vitro do porta-enxerto de videira 'VR043-43' (Vitis vinifera x Vitis rotundifolia)Multiplication in vitro of grapevine rootstock 'VR043–43' (Vitis vinifera x Vitis rotundifolia)CitocininaMicropropagaçãoEnraizamentoVitisCytokininMicropropagationRootingThe micropropagation is one technique that makes possible the massal propagation of plants of economic interest. The objective of this work was to evaluate the effect of 6–benzilaminopurina (BAP) and kinetin (KIN) in the multiplication in vitro of the grapevine rootstock 'VR043–43'. The isolation was carried through in culture medium QL, supplemented with BAP or KIN, in the concentrations of 0; 2,5; 5,0 and 10,0µM. The subcultures had been carried out each 45 days. The number of shoots per explant increased along with the subcultures with BAP. However this effect was not observed with the KIN that it got resulted similar to the one of the cytokin–free (1,0 shoot/explant). The concentrations of 5,0 nd 10,0 µM of BAP had promoted the biggest number of shoots with reduction of the height. The highest concentration of KIN (10,0 µM) also had negative effect in the height of the shoots, as well as, in the number of leaves per shoot. The callus formation (100%) was observed with BAP and KIN in all the concentrations, and in the cytokinin absence it did not have the callus formation. Cytokinin BAP reduced the formation of roots that was of 100% with the KIN and cytokinin–free.A micropropagação é uma técnica que possibilita a propagação massal de plantas de interesse econômico. Objetivou-se com este trabalho avaliar o efeito de 6-benzilaminopurina (BAP) e cinetina (CIN) na multiplicação do porta-enxerto de videira 'VR043-43'. O isolamento foi realizado em meio de cultura QL, suplementado com BAP ou CIN, nas concentrações de 0; 2,5; 5,0 e 10,0µM. Os subcultivos foram realizados a cada 45 dias. O número de brotações por explante aumentou ao longo dos subcultivos com BAP. Esse efeito não foi observado com a CIN, que obteve resultado semelhante ao da testemunha (1,0 brotação/explante). As concentrações de 5,0 e 10,0 µM de BAP promoveram o maior número de brotações, porém com redução da altura. A concentração mais elevada de CIN (10,0 µM), também teve efeito negativo na altura das brotações, assim como no número de folhas por brotação. A formação de calo (100%) foi observada com BAP e CIN em todas as concentrações, e na ausência de citocinina não houve a formação de calo. A citocinina BAP reduziu a formação de raízes, que foi de 100% com a CIN e a testemunha.Editora da Universidade Federal de Lavras2006-08-012015-04-30T13:33:49Z2015-04-30T13:33:49Z2015-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000400009MACHADO, M. P. et al. Multiplicação in vitro do porta-enxerto de videira 'VR043-43' (Vitis vinifera x Vitis rotundifolia). Ciência e Agrotecnologia, Lavras, v. 30, n. 4, p. 648-655, jul./ago. 2006.http://repositorio.ufla.br/jspui/handle/1/6244Ciência e Agrotecnologia v.30 n.4 2006reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAMachado, Marília PereiraBiasi, Luiz AntonioRitter, MarliceRibas, Luciana Lopes FortesKoehler, Henrique Soaresporinfo:eu-repo/semantics/openAccess2016-07-22T18:47:21Zoai:localhost:1/6244Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2016-07-22T18:47:21Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Multiplicação in vitro do porta-enxerto de videira 'VR043-43' (Vitis vinifera x Vitis rotundifolia) Multiplication in vitro of grapevine rootstock 'VR043–43' (Vitis vinifera x Vitis rotundifolia) |
title |
Multiplicação in vitro do porta-enxerto de videira 'VR043-43' (Vitis vinifera x Vitis rotundifolia) |
spellingShingle |
Multiplicação in vitro do porta-enxerto de videira 'VR043-43' (Vitis vinifera x Vitis rotundifolia) Machado, Marília Pereira Citocinina Micropropagação Enraizamento Vitis Cytokinin Micropropagation Rooting |
title_short |
Multiplicação in vitro do porta-enxerto de videira 'VR043-43' (Vitis vinifera x Vitis rotundifolia) |
title_full |
Multiplicação in vitro do porta-enxerto de videira 'VR043-43' (Vitis vinifera x Vitis rotundifolia) |
title_fullStr |
Multiplicação in vitro do porta-enxerto de videira 'VR043-43' (Vitis vinifera x Vitis rotundifolia) |
title_full_unstemmed |
Multiplicação in vitro do porta-enxerto de videira 'VR043-43' (Vitis vinifera x Vitis rotundifolia) |
title_sort |
Multiplicação in vitro do porta-enxerto de videira 'VR043-43' (Vitis vinifera x Vitis rotundifolia) |
author |
Machado, Marília Pereira |
author_facet |
Machado, Marília Pereira Biasi, Luiz Antonio Ritter, Marlice Ribas, Luciana Lopes Fortes Koehler, Henrique Soares |
author_role |
author |
author2 |
Biasi, Luiz Antonio Ritter, Marlice Ribas, Luciana Lopes Fortes Koehler, Henrique Soares |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Machado, Marília Pereira Biasi, Luiz Antonio Ritter, Marlice Ribas, Luciana Lopes Fortes Koehler, Henrique Soares |
dc.subject.por.fl_str_mv |
Citocinina Micropropagação Enraizamento Vitis Cytokinin Micropropagation Rooting |
topic |
Citocinina Micropropagação Enraizamento Vitis Cytokinin Micropropagation Rooting |
description |
The micropropagation is one technique that makes possible the massal propagation of plants of economic interest. The objective of this work was to evaluate the effect of 6–benzilaminopurina (BAP) and kinetin (KIN) in the multiplication in vitro of the grapevine rootstock 'VR043–43'. The isolation was carried through in culture medium QL, supplemented with BAP or KIN, in the concentrations of 0; 2,5; 5,0 and 10,0µM. The subcultures had been carried out each 45 days. The number of shoots per explant increased along with the subcultures with BAP. However this effect was not observed with the KIN that it got resulted similar to the one of the cytokin–free (1,0 shoot/explant). The concentrations of 5,0 nd 10,0 µM of BAP had promoted the biggest number of shoots with reduction of the height. The highest concentration of KIN (10,0 µM) also had negative effect in the height of the shoots, as well as, in the number of leaves per shoot. The callus formation (100%) was observed with BAP and KIN in all the concentrations, and in the cytokinin absence it did not have the callus formation. Cytokinin BAP reduced the formation of roots that was of 100% with the KIN and cytokinin–free. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-08-01 2015-04-30T13:33:49Z 2015-04-30T13:33:49Z 2015-04-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000400009 MACHADO, M. P. et al. Multiplicação in vitro do porta-enxerto de videira 'VR043-43' (Vitis vinifera x Vitis rotundifolia). Ciência e Agrotecnologia, Lavras, v. 30, n. 4, p. 648-655, jul./ago. 2006. http://repositorio.ufla.br/jspui/handle/1/6244 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542006000400009 http://repositorio.ufla.br/jspui/handle/1/6244 |
identifier_str_mv |
MACHADO, M. P. et al. Multiplicação in vitro do porta-enxerto de videira 'VR043-43' (Vitis vinifera x Vitis rotundifolia). Ciência e Agrotecnologia, Lavras, v. 30, n. 4, p. 648-655, jul./ago. 2006. |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
dc.source.none.fl_str_mv |
Ciência e Agrotecnologia v.30 n.4 2006 reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
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UFLA |
reponame_str |
Repositório Institucional da UFLA |
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Repositório Institucional da UFLA |
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Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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