Osmolaridade e taxa de diluição na ativação do sêmen criopreservado de Prochilodus lineatus

Detalhes bibliográficos
Autor(a) principal: Felizardo, Viviane de Oliveira
Data de Publicação: 2011
Outros Autores: Murgas, Luis David Solis, Navarro, Rodrigo Diana, Gonçalves, Antônio Carlos Silveira, Paulino, Michelle Sampaio
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/39183
Resumo: The objective of this work was to evaluate the influence of activators with different osmolarities and dilution rate in the activation of cryopreserved semen of curimba (Prochilodus lineatus). We used four breeding males captured in the Fishfarming Station of CEMIG, Itutinga, MG. Semen samples were diluted in two solutions (DMSO + methanol and lactose + lactose) at 1:4 ratio and frozen. The rates (%) and duration (s) of sperm motility for treatments were assessed. Activators with respective osmolarities: 30, 99, 183 and 293 mOsm at dilution rates of 1:2, 1:4, 1:6, 1:8 (semen: activator) were used. The dilution rates of 1:2 and 1:8 influenced duration of motility in semen cryopreserved with DMSO. For the 1:2 dilution, the semen activated with the activator containing 30 mOsm, presented greater motility duration than semen activated with activators with 183 and 293 mOsm. However, for the activator with 30 mOsm, it was obtained longer motility duration at a dilution of 1:2 compared to 1:4 dilution. At 1:6 dilution rate, when the semen was activated with the activator 30 mOsm, the motility rate was higher than that provided by the semen that was activated with 293 mOsm. At the dilution rate of 1:8, the use of activator containing 99 mOsm showed longer motility duration in relation to use of the activator with 293 mOsm. Activators containing high osmolarities can provide lower rates and duration of motility in semen of curimba (Prochilodus lineatus) cryopreserved with cryoprotectants methanol or DMSO.
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spelling Osmolaridade e taxa de diluição na ativação do sêmen criopreservado de Prochilodus lineatusOsmolarity of activator and dilution rate in the activation of cryopreserved semen of curimba (Prochilodus lineatus)Peixe - ReproduçãoMotilidade espermáticaFish - ReproductionSperm motilityCurimbaThe objective of this work was to evaluate the influence of activators with different osmolarities and dilution rate in the activation of cryopreserved semen of curimba (Prochilodus lineatus). We used four breeding males captured in the Fishfarming Station of CEMIG, Itutinga, MG. Semen samples were diluted in two solutions (DMSO + methanol and lactose + lactose) at 1:4 ratio and frozen. The rates (%) and duration (s) of sperm motility for treatments were assessed. Activators with respective osmolarities: 30, 99, 183 and 293 mOsm at dilution rates of 1:2, 1:4, 1:6, 1:8 (semen: activator) were used. The dilution rates of 1:2 and 1:8 influenced duration of motility in semen cryopreserved with DMSO. For the 1:2 dilution, the semen activated with the activator containing 30 mOsm, presented greater motility duration than semen activated with activators with 183 and 293 mOsm. However, for the activator with 30 mOsm, it was obtained longer motility duration at a dilution of 1:2 compared to 1:4 dilution. At 1:6 dilution rate, when the semen was activated with the activator 30 mOsm, the motility rate was higher than that provided by the semen that was activated with 293 mOsm. At the dilution rate of 1:8, the use of activator containing 99 mOsm showed longer motility duration in relation to use of the activator with 293 mOsm. Activators containing high osmolarities can provide lower rates and duration of motility in semen of curimba (Prochilodus lineatus) cryopreserved with cryoprotectants methanol or DMSO.Objetivou-se avaliar a influência de ativadores com diferentes osmolaridades e taxa de diluição na ativação de sêmen criopreservado de curimba (Prochilodus lineatus). Foram utilizados quatro reprodutores machos capturados na Estação de Piscicultura da CEMIG, Itutinga, MG. As amostras de sêmen foram diluídas em duas soluções (DMSO + lactose e metanol + lactose) na proporção de 1:4 e congeladas. Foram avaliadas as taxas (%) e duração (s) da motilidade espermática dos tratamentos. Na ativação foram utilizados ativadores contendo as respectivas osmolaridades: 30, 99, 183 e 293 mOsm nas taxas de diluição de 1:2, 1:4, 1:6, 1:8 sêmen: ativador. A taxa de diluição 1:2 e 1:8 foram estatisticamente significativas para duração da motilidade para o sêmen criopreservado com DMSO. Para a diluição 1:2, o sêmen ativado com o ativador contendo 30 mOsm, apresentou duração de motilidade maior em relação ao sêmen ativado com ativadores com 183 e 293 mOsm. No entanto, para a utilização do ativador com 30 mOsm, se obteve maior duração da motilidade na diluição de 1:2 em relação a diluição de 1:4. Na taxa de diluição 1:6, quando o sêmen foi ativado com o ativador de 30 mOsm, a taxa de motilidade foi superior à apresentada pelo sêmen que foi ativado com 293 mOsm. Na taxa de diluição de 1:8, o uso de ativador contendo 99 mOsm apresentou maior duração de motilidade em relação ao uso do ativador com 293 mOsm. Ativadores contendo altas osmolaridades podem proporcionar menores taxas e duração da motilidade em sêmen de curimba (Prochilodus lineatus) criopreservado com os crioprotetores metanol ou DMSO.Universidad de Córdoba2020-03-05T17:57:43Z2020-03-05T17:57:43Z2011-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfFELIZARDO, V. O. et al. Osmolaridade e taxa de diluição na ativação do sêmen criopreservado de Prochilodus lineatus. Archivos de Zootecnia, Córdoba, v. 60, n. 232, p. 1255-1262, dic. 2011.http://repositorio.ufla.br/jspui/handle/1/39183Archivos de Zootecniareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessFelizardo, Viviane de OliveiraMurgas, Luis David SolisNavarro, Rodrigo DianaGonçalves, Antônio Carlos SilveiraPaulino, Michelle Sampaiopor2020-03-05T17:59:50Zoai:localhost:1/39183Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-03-05T17:59:50Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Osmolaridade e taxa de diluição na ativação do sêmen criopreservado de Prochilodus lineatus
Osmolarity of activator and dilution rate in the activation of cryopreserved semen of curimba (Prochilodus lineatus)
title Osmolaridade e taxa de diluição na ativação do sêmen criopreservado de Prochilodus lineatus
spellingShingle Osmolaridade e taxa de diluição na ativação do sêmen criopreservado de Prochilodus lineatus
Felizardo, Viviane de Oliveira
Peixe - Reprodução
Motilidade espermática
Fish - Reproduction
Sperm motility
Curimba
title_short Osmolaridade e taxa de diluição na ativação do sêmen criopreservado de Prochilodus lineatus
title_full Osmolaridade e taxa de diluição na ativação do sêmen criopreservado de Prochilodus lineatus
title_fullStr Osmolaridade e taxa de diluição na ativação do sêmen criopreservado de Prochilodus lineatus
title_full_unstemmed Osmolaridade e taxa de diluição na ativação do sêmen criopreservado de Prochilodus lineatus
title_sort Osmolaridade e taxa de diluição na ativação do sêmen criopreservado de Prochilodus lineatus
author Felizardo, Viviane de Oliveira
author_facet Felizardo, Viviane de Oliveira
Murgas, Luis David Solis
Navarro, Rodrigo Diana
Gonçalves, Antônio Carlos Silveira
Paulino, Michelle Sampaio
author_role author
author2 Murgas, Luis David Solis
Navarro, Rodrigo Diana
Gonçalves, Antônio Carlos Silveira
Paulino, Michelle Sampaio
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Felizardo, Viviane de Oliveira
Murgas, Luis David Solis
Navarro, Rodrigo Diana
Gonçalves, Antônio Carlos Silveira
Paulino, Michelle Sampaio
dc.subject.por.fl_str_mv Peixe - Reprodução
Motilidade espermática
Fish - Reproduction
Sperm motility
Curimba
topic Peixe - Reprodução
Motilidade espermática
Fish - Reproduction
Sperm motility
Curimba
description The objective of this work was to evaluate the influence of activators with different osmolarities and dilution rate in the activation of cryopreserved semen of curimba (Prochilodus lineatus). We used four breeding males captured in the Fishfarming Station of CEMIG, Itutinga, MG. Semen samples were diluted in two solutions (DMSO + methanol and lactose + lactose) at 1:4 ratio and frozen. The rates (%) and duration (s) of sperm motility for treatments were assessed. Activators with respective osmolarities: 30, 99, 183 and 293 mOsm at dilution rates of 1:2, 1:4, 1:6, 1:8 (semen: activator) were used. The dilution rates of 1:2 and 1:8 influenced duration of motility in semen cryopreserved with DMSO. For the 1:2 dilution, the semen activated with the activator containing 30 mOsm, presented greater motility duration than semen activated with activators with 183 and 293 mOsm. However, for the activator with 30 mOsm, it was obtained longer motility duration at a dilution of 1:2 compared to 1:4 dilution. At 1:6 dilution rate, when the semen was activated with the activator 30 mOsm, the motility rate was higher than that provided by the semen that was activated with 293 mOsm. At the dilution rate of 1:8, the use of activator containing 99 mOsm showed longer motility duration in relation to use of the activator with 293 mOsm. Activators containing high osmolarities can provide lower rates and duration of motility in semen of curimba (Prochilodus lineatus) cryopreserved with cryoprotectants methanol or DMSO.
publishDate 2011
dc.date.none.fl_str_mv 2011-12
2020-03-05T17:57:43Z
2020-03-05T17:57:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv FELIZARDO, V. O. et al. Osmolaridade e taxa de diluição na ativação do sêmen criopreservado de Prochilodus lineatus. Archivos de Zootecnia, Córdoba, v. 60, n. 232, p. 1255-1262, dic. 2011.
http://repositorio.ufla.br/jspui/handle/1/39183
identifier_str_mv FELIZARDO, V. O. et al. Osmolaridade e taxa de diluição na ativação do sêmen criopreservado de Prochilodus lineatus. Archivos de Zootecnia, Córdoba, v. 60, n. 232, p. 1255-1262, dic. 2011.
url http://repositorio.ufla.br/jspui/handle/1/39183
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dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad de Córdoba
publisher.none.fl_str_mv Universidad de Córdoba
dc.source.none.fl_str_mv Archivos de Zootecnia
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
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instname_str Universidade Federal de Lavras (UFLA)
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institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
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