Selection of elite rhizobia strains by biometric techniques for inoculation in cowpea and common-bean

Detalhes bibliográficos
Autor(a) principal: Oliveira, Dâmiany Pádua
Data de Publicação: 2021
Tipo de documento: Tese
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/48377
Resumo: Plant breeding programs seek to obtain genotypes with high yield, yield stability, and wide adaptability to growing environments. The selection of genotypes of microorganisms for adoption as agricultural inoculants has a similar objective. In both cases, the genotype-environment interaction makes it difficult to select materials adapted to highly diverse edaphic and climatic conditions. However, with suitable biometric techniques, variation in the response of each genotype in the environments can be analyzed, allowing selection of those with the desired standard and type of response. For that purpose, Toler and AMMI (additive main effect and multiplicative interaction) modeling and the Annicchiarico methodology were used together with genotypes of nitrogen fixing bacteria in symbiosis with cowpea (Vigna unguiculata) and common bean (Phaseolus vulgaris) in seven and eight environments, respectively, to determine their adaptabilities and phenotypic stabilities. The response patterns of the symbiont strains for common bean were furthermore determined in four plant cultivars, which composed the 16 macroenvironmental components of the genotype(G)- cultivar(C)- edaphoclimatic environment(E) interaction (GCE interaction). This research showed that the Toler and AMMI models and the Annicchiarico method accurately estimated the effects of adaptability and phenotypic stability of nitrogen fixing bacteria symbiont strains for inoculation in cowpea and common-bean. High adaptability and the potentials of phenotypic stabilities of the genotypes INPA03-11B (of Bradyrhizobium elkanii) and UFLA03-164 (B. uaiense) for cowpea and UFLA02-127 (of Rhizobium sp.) for common bean were confirmed. They were the ones that least contributed to the interaction, with yield capacity equivalent to that from fertilization with high doses of mineral N. They have a foreseeable response and ability to benefit from positive environmental stimuli, but are little to not at all affected by environments of unfavorable quality. They exhibit characteristics idealized by soil microbiologists, by inoculant producers, and by farmers that desire satisfactory yields, with minimal oscillation (under high stability), even with low inputs. INPA03-11B has already been approved as an inoculant by the Brazilian Ministry of Agriculture (MAPA); UFLA03-164 and UFLA02-127 have potential for inclusion as inoculants approved for commercial use for cowpea and common bean, respectively. In addition to UFLA02-127, whose good performance does not depend on the environmental condition and the host cultivar, other strains of Rhizobium under the UFLA code tested have yield capacity and adaptabilities higher than those of the CIAT899 strain (of Rhizobium tropici) currently approved as an inoculant for common bean in Brazil. The results from CIAT899, however, are enhanced under more stressful conditions and when the host is the Madrepérola cultivar. This was the first time that these biometric techniques were applied for rhizobia selection in field studies. It is recommended that these biometric techniques be used simultaneously for approval of new strains.
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spelling Selection of elite rhizobia strains by biometric techniques for inoculation in cowpea and common-beanRhizobiumBradyrhizobiumAdaptabilidadeEstabilidade fenotípicaModelos Toler e AMMIMétodo AnnicchiaricoFixação biológica de nitrogênioPhaseolus vulgarisVigna unguiculataAdaptabilityPhenotypic stabilityToler and AMMI modelsAnnicchiarico methodBiological nitrogen fixationCiência do SoloPlant breeding programs seek to obtain genotypes with high yield, yield stability, and wide adaptability to growing environments. The selection of genotypes of microorganisms for adoption as agricultural inoculants has a similar objective. In both cases, the genotype-environment interaction makes it difficult to select materials adapted to highly diverse edaphic and climatic conditions. However, with suitable biometric techniques, variation in the response of each genotype in the environments can be analyzed, allowing selection of those with the desired standard and type of response. For that purpose, Toler and AMMI (additive main effect and multiplicative interaction) modeling and the Annicchiarico methodology were used together with genotypes of nitrogen fixing bacteria in symbiosis with cowpea (Vigna unguiculata) and common bean (Phaseolus vulgaris) in seven and eight environments, respectively, to determine their adaptabilities and phenotypic stabilities. The response patterns of the symbiont strains for common bean were furthermore determined in four plant cultivars, which composed the 16 macroenvironmental components of the genotype(G)- cultivar(C)- edaphoclimatic environment(E) interaction (GCE interaction). This research showed that the Toler and AMMI models and the Annicchiarico method accurately estimated the effects of adaptability and phenotypic stability of nitrogen fixing bacteria symbiont strains for inoculation in cowpea and common-bean. High adaptability and the potentials of phenotypic stabilities of the genotypes INPA03-11B (of Bradyrhizobium elkanii) and UFLA03-164 (B. uaiense) for cowpea and UFLA02-127 (of Rhizobium sp.) for common bean were confirmed. They were the ones that least contributed to the interaction, with yield capacity equivalent to that from fertilization with high doses of mineral N. They have a foreseeable response and ability to benefit from positive environmental stimuli, but are little to not at all affected by environments of unfavorable quality. They exhibit characteristics idealized by soil microbiologists, by inoculant producers, and by farmers that desire satisfactory yields, with minimal oscillation (under high stability), even with low inputs. INPA03-11B has already been approved as an inoculant by the Brazilian Ministry of Agriculture (MAPA); UFLA03-164 and UFLA02-127 have potential for inclusion as inoculants approved for commercial use for cowpea and common bean, respectively. In addition to UFLA02-127, whose good performance does not depend on the environmental condition and the host cultivar, other strains of Rhizobium under the UFLA code tested have yield capacity and adaptabilities higher than those of the CIAT899 strain (of Rhizobium tropici) currently approved as an inoculant for common bean in Brazil. The results from CIAT899, however, are enhanced under more stressful conditions and when the host is the Madrepérola cultivar. This was the first time that these biometric techniques were applied for rhizobia selection in field studies. It is recommended that these biometric techniques be used simultaneously for approval of new strains.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Os programas de melhoramento de plantas procuram obter genótipos com alto rendimento, estabilidade de rendimento e ampla adaptabilidade a ambientes de cultivo. A seleção de genótipos de microrganismos para adoção como inoculante agrícola tem objetivo semelhante. Em ambos os casos, a interação genótipo-ambiente torna difícil selecionar materiais adaptados a condições edáficas e climáticas altamente diversas. Porém, com técnicas biométricas adequadas, a variação na resposta de cada genótipo nos ambientes pode ser analisada, permitindo a seleção daqueles com o padrão e tipo de resposta desejados. Para tanto, foram utilizados os modelos de Toler e AMMI (efeitos principais aditivos e análise de interação multiplicativa) e a metodologia Annicchiarico em conjunto com genótipos de bactérias fixadoras de nitrogênio em simbiose com feijão-caupi (Vigna unguiculata) e feijão (Phaseolus vulgaris) em sete e oito. ambientes, respectivamente, para determinar suas adaptabilidades e estabilidades fenotípicas. Além disso, os padrões de resposta das linhagens simbiontes para o feijoeiro foram determinados em quatro cultivares de plantas, que compunham os 16 componentes macroambientais da interação genótipo (G) - cultivar (C) - ambiente edafoclimático (E) (interação GCE). Esta pesquisa mostrou que os modelos Toler e AMMI e o método Annicchiarico estimaram com precisão os efeitos da adaptabilidade e estabilidade fenotípica de estirpes simbiontes de bactérias fixadoras de nitrogênio para inoculação em feijão-caupi e feijão. A alta adaptabilidade e os potenciais de estabilidades fenotípicas dos genótipos INPA03-11B (de Bradyrhizobium elkanii) e UFLA03-164 (B. uaiense) para caupi e UFLA02-127 (de Rhizobium sp.) para feijão-comum foram confirmados. Eles foram os que menos contribuíram para a interação, com capacidade produtiva equivalente à da fertilização com altas doses do mineral N. Eles têm uma resposta previsível e capacidade de se beneficiar de estímulos ambientais positivos, mas são pouco ou nada afetados pelos ambientes de qualidade desfavorável. Exibem características idealizadas por microbiologistas de solo, por produtores de inoculantes e por agricultores que desejam rendimentos satisfatórios, com oscilação mínima (em alta estabilidade), mesmo com baixos insumos. O INPA03-11B já foi aprovado como inoculante pelo Ministério da Agricultura do Brasil (MAPA); UFLA03-164 e UFLA02-127 têm potencial para inclusão como inoculantes aprovados para uso comercial para caupi e feijão-comum, respectivamente. Além da UFLA02-127, cujo bom desempenho independe das condições ambientais e da cultivar hospedeira, outras estirpes de Rhizobium sob o código UFLA testadas apresentam capacidade produtiva e adaptabilidades superiores às da CIAT899 (de Rhizobium tropici) atualmente aprovada como inoculante para feijão-comum no Brasil. Os resultados do CIAT899, entretanto, são potencializados em condições mais estressantes e quando o hospedeiro é a cultivar Madrepérola. Esta foi a primeira vez que essas técnicas biométricas foram aplicadas para a seleção de rizóbios em estudos de campo. Recomenda-se que essas técnicas biométricas sejam utilizadas simultaneamente para aprovação de novas estirpes.Universidade Federal de LavrasPrograma de Pós-Graduação em Ciência do SoloUFLAbrasilDepartamento de Ciência do SoloMoreira, Fatima Maria de SouzaFerreira, Daniel FurtadoCosta, Elaine Martins daLeite, JaksonFerreira, Paulo Ademar AvelarNóbrega, Rafaela Simão AbrahãoFurtado, Daniel FerreiraOliveira, Dâmiany Pádua2021-10-15T19:56:34Z2021-10-15T19:56:34Z2021-10-152021-08-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfOLIVEIRA, D. P. Selection of elite rhizobia strains by biometric techniques for inoculation in cowpea and common-bean. 2021. 103 p. Tese (Doutorado em Ciência do Solo) – Universidade Federal de Lavras, Lavras, 2021.http://repositorio.ufla.br/jspui/handle/1/48377enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2021-10-15T19:56:35Zoai:localhost:1/48377Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2021-10-15T19:56:35Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Selection of elite rhizobia strains by biometric techniques for inoculation in cowpea and common-bean
title Selection of elite rhizobia strains by biometric techniques for inoculation in cowpea and common-bean
spellingShingle Selection of elite rhizobia strains by biometric techniques for inoculation in cowpea and common-bean
Oliveira, Dâmiany Pádua
Rhizobium
Bradyrhizobium
Adaptabilidade
Estabilidade fenotípica
Modelos Toler e AMMI
Método Annicchiarico
Fixação biológica de nitrogênio
Phaseolus vulgaris
Vigna unguiculata
Adaptability
Phenotypic stability
Toler and AMMI models
Annicchiarico method
Biological nitrogen fixation
Ciência do Solo
title_short Selection of elite rhizobia strains by biometric techniques for inoculation in cowpea and common-bean
title_full Selection of elite rhizobia strains by biometric techniques for inoculation in cowpea and common-bean
title_fullStr Selection of elite rhizobia strains by biometric techniques for inoculation in cowpea and common-bean
title_full_unstemmed Selection of elite rhizobia strains by biometric techniques for inoculation in cowpea and common-bean
title_sort Selection of elite rhizobia strains by biometric techniques for inoculation in cowpea and common-bean
author Oliveira, Dâmiany Pádua
author_facet Oliveira, Dâmiany Pádua
author_role author
dc.contributor.none.fl_str_mv Moreira, Fatima Maria de Souza
Ferreira, Daniel Furtado
Costa, Elaine Martins da
Leite, Jakson
Ferreira, Paulo Ademar Avelar
Nóbrega, Rafaela Simão Abrahão
Furtado, Daniel Ferreira
dc.contributor.author.fl_str_mv Oliveira, Dâmiany Pádua
dc.subject.por.fl_str_mv Rhizobium
Bradyrhizobium
Adaptabilidade
Estabilidade fenotípica
Modelos Toler e AMMI
Método Annicchiarico
Fixação biológica de nitrogênio
Phaseolus vulgaris
Vigna unguiculata
Adaptability
Phenotypic stability
Toler and AMMI models
Annicchiarico method
Biological nitrogen fixation
Ciência do Solo
topic Rhizobium
Bradyrhizobium
Adaptabilidade
Estabilidade fenotípica
Modelos Toler e AMMI
Método Annicchiarico
Fixação biológica de nitrogênio
Phaseolus vulgaris
Vigna unguiculata
Adaptability
Phenotypic stability
Toler and AMMI models
Annicchiarico method
Biological nitrogen fixation
Ciência do Solo
description Plant breeding programs seek to obtain genotypes with high yield, yield stability, and wide adaptability to growing environments. The selection of genotypes of microorganisms for adoption as agricultural inoculants has a similar objective. In both cases, the genotype-environment interaction makes it difficult to select materials adapted to highly diverse edaphic and climatic conditions. However, with suitable biometric techniques, variation in the response of each genotype in the environments can be analyzed, allowing selection of those with the desired standard and type of response. For that purpose, Toler and AMMI (additive main effect and multiplicative interaction) modeling and the Annicchiarico methodology were used together with genotypes of nitrogen fixing bacteria in symbiosis with cowpea (Vigna unguiculata) and common bean (Phaseolus vulgaris) in seven and eight environments, respectively, to determine their adaptabilities and phenotypic stabilities. The response patterns of the symbiont strains for common bean were furthermore determined in four plant cultivars, which composed the 16 macroenvironmental components of the genotype(G)- cultivar(C)- edaphoclimatic environment(E) interaction (GCE interaction). This research showed that the Toler and AMMI models and the Annicchiarico method accurately estimated the effects of adaptability and phenotypic stability of nitrogen fixing bacteria symbiont strains for inoculation in cowpea and common-bean. High adaptability and the potentials of phenotypic stabilities of the genotypes INPA03-11B (of Bradyrhizobium elkanii) and UFLA03-164 (B. uaiense) for cowpea and UFLA02-127 (of Rhizobium sp.) for common bean were confirmed. They were the ones that least contributed to the interaction, with yield capacity equivalent to that from fertilization with high doses of mineral N. They have a foreseeable response and ability to benefit from positive environmental stimuli, but are little to not at all affected by environments of unfavorable quality. They exhibit characteristics idealized by soil microbiologists, by inoculant producers, and by farmers that desire satisfactory yields, with minimal oscillation (under high stability), even with low inputs. INPA03-11B has already been approved as an inoculant by the Brazilian Ministry of Agriculture (MAPA); UFLA03-164 and UFLA02-127 have potential for inclusion as inoculants approved for commercial use for cowpea and common bean, respectively. In addition to UFLA02-127, whose good performance does not depend on the environmental condition and the host cultivar, other strains of Rhizobium under the UFLA code tested have yield capacity and adaptabilities higher than those of the CIAT899 strain (of Rhizobium tropici) currently approved as an inoculant for common bean in Brazil. The results from CIAT899, however, are enhanced under more stressful conditions and when the host is the Madrepérola cultivar. This was the first time that these biometric techniques were applied for rhizobia selection in field studies. It is recommended that these biometric techniques be used simultaneously for approval of new strains.
publishDate 2021
dc.date.none.fl_str_mv 2021-10-15T19:56:34Z
2021-10-15T19:56:34Z
2021-10-15
2021-08-20
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv OLIVEIRA, D. P. Selection of elite rhizobia strains by biometric techniques for inoculation in cowpea and common-bean. 2021. 103 p. Tese (Doutorado em Ciência do Solo) – Universidade Federal de Lavras, Lavras, 2021.
http://repositorio.ufla.br/jspui/handle/1/48377
identifier_str_mv OLIVEIRA, D. P. Selection of elite rhizobia strains by biometric techniques for inoculation in cowpea and common-bean. 2021. 103 p. Tese (Doutorado em Ciência do Solo) – Universidade Federal de Lavras, Lavras, 2021.
url http://repositorio.ufla.br/jspui/handle/1/48377
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Lavras
Programa de Pós-Graduação em Ciência do Solo
UFLA
brasil
Departamento de Ciência do Solo
publisher.none.fl_str_mv Universidade Federal de Lavras
Programa de Pós-Graduação em Ciência do Solo
UFLA
brasil
Departamento de Ciência do Solo
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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