Impacto da Mastite bovina por Sthapylococcus aureus e Streptococcus agalactiae

Detalhes bibliográficos
Autor(a) principal: Mesquita, Alan Andrade
Data de Publicação: 2017
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/28358
Resumo: Bovine mastitis is the most important disease in dairy herds worldwide, causing great health and economic losses to producers and industry, as well as having implications for public health due to the zoonotic potential. Considering the multifactorial aspect of this disease, the knowledge of the agents involved in its etiology, antimicrobial susceptibility profiles, its economic impact on the production and the risk factors involved in its occurrence has high importance for its control. The experiment was carried out in partnership with a dairy industry in the region of Lavras/South of Minas Gerais, which at that time had 306 milk supply properties. In the first study, the impact of bovine mastitis on milk production was evaluated. In order to calculate the production losses, the means of three BMSCC of the 306 properties, obtained at intervals of 15 days between the collections, were used, and losses were estimated through the linear regression equation y = -4.7908 + 0.0226x, where the variable "x" represents the BMSCC and “y” the estimates of milk percentage losses. The mean of BMSCC between the studied properties was 482,650 cells/mL, and 43,710 CFU/mL of milk for total bacterial counts (TBC), with 35.6% and 7.84% of the farms above of limits established by current legislation for BMSCC and TBC, respectively. The results showed that the average daily losses in production due to mastitis were 6.27%. In the second study, the prevalence of Staphylococcus aureus and Streptococcus agalactiae was evaluated, by means of microbiological analyzes of milk samples collected from bulk milk tanks, and their relation with the BMSCC, TBC and daily production. Additionally, the resistance profile of S. aureus (99) and S. agalactiae (93) strains to antimicrobials was evaluated by disc diffusion technique. Our results showed high prevalences of S. aureus (70.3%) and S. agalactiae pathogens (67.0%), and associations between BMSCC and the presence of S. aureus and S. agalactiae pathogens and between the TBC and the presence of S. agalactiae, demonstrating the negative interference of these pathogens in these milk quality aspects. High levels of resistance and multiresistance were observed for S. aureus and S. agalactiae in the st udied herds. In the third study, a case-control study was carried out involving 46 dairy farms, of which 30 were considered as cases (CCSt ≥ 700,000 cells/mL of milk) and 16 as controls (CCSt ≤ 200,000 cells/mL of milk). Sixteen qualitative and four quantitative variables were analyzed. The results showed that the main risk factors for high BMSCC were the presence of the pathogens S. aureus and S. agalactiae, non-use of pre and post-dipping, non-use of paper towel for drying teats, non-use herd monitoring by California Mastitis Tests (CMT) or individual SCC, absence of the milking line and the therapy of dry cows, and failures in hygiene of teats and udders before milking. Failures related to maintenance and use of milking equipment, including the application of manual pressure to milking assemblies, unregulated vacuum pressure in milking machine, loss of vacuum during milking, and maintenance failures of milking machine and bulk milk tank were also found as relevant risk factors. Moderate correlations were a lso observed between elevation of BMSCC and counts of S. aureus and S. agalactiae in the bulk milk tank and moderate correlation between counts of S. aureus and counts of S. agalactiae. Our results showed high levels of production losses and prevalence of S. aureus and S. agalactiae pathogens, with high scores of antimicrobial resistance in the studied herds. The risk factors for BMSCC ≥ 700,000 cells/mL of milk were related to failures in relation to the classic measures of control of bovine mastitis, emphasizing the importance of the same to improve milk quality and mitigate the losses caused by this disease to producers and industry.
id UFLA_717d0de73170cd369439037025fa12a8
oai_identifier_str oai:localhost:1/28358
network_acronym_str UFLA
network_name_str Repositório Institucional da UFLA
repository_id_str
spelling Impacto da Mastite bovina por Sthapylococcus aureus e Streptococcus agalactiaeImpact of bovine Mastitis by Staphylococcus aureus and Streptococcus agalactiaeMastite bovinaMastite - CustosMastite - ControleBovinos leiteiros - DoençasContagem bacteriana totalBovine mastitisMastitis - CostsMastitis - ControlDairy cattle - DiseasesTotal bacterial countMedicina VeterináriaBovine mastitis is the most important disease in dairy herds worldwide, causing great health and economic losses to producers and industry, as well as having implications for public health due to the zoonotic potential. Considering the multifactorial aspect of this disease, the knowledge of the agents involved in its etiology, antimicrobial susceptibility profiles, its economic impact on the production and the risk factors involved in its occurrence has high importance for its control. The experiment was carried out in partnership with a dairy industry in the region of Lavras/South of Minas Gerais, which at that time had 306 milk supply properties. In the first study, the impact of bovine mastitis on milk production was evaluated. In order to calculate the production losses, the means of three BMSCC of the 306 properties, obtained at intervals of 15 days between the collections, were used, and losses were estimated through the linear regression equation y = -4.7908 + 0.0226x, where the variable "x" represents the BMSCC and “y” the estimates of milk percentage losses. The mean of BMSCC between the studied properties was 482,650 cells/mL, and 43,710 CFU/mL of milk for total bacterial counts (TBC), with 35.6% and 7.84% of the farms above of limits established by current legislation for BMSCC and TBC, respectively. The results showed that the average daily losses in production due to mastitis were 6.27%. In the second study, the prevalence of Staphylococcus aureus and Streptococcus agalactiae was evaluated, by means of microbiological analyzes of milk samples collected from bulk milk tanks, and their relation with the BMSCC, TBC and daily production. Additionally, the resistance profile of S. aureus (99) and S. agalactiae (93) strains to antimicrobials was evaluated by disc diffusion technique. Our results showed high prevalences of S. aureus (70.3%) and S. agalactiae pathogens (67.0%), and associations between BMSCC and the presence of S. aureus and S. agalactiae pathogens and between the TBC and the presence of S. agalactiae, demonstrating the negative interference of these pathogens in these milk quality aspects. High levels of resistance and multiresistance were observed for S. aureus and S. agalactiae in the st udied herds. In the third study, a case-control study was carried out involving 46 dairy farms, of which 30 were considered as cases (CCSt ≥ 700,000 cells/mL of milk) and 16 as controls (CCSt ≤ 200,000 cells/mL of milk). Sixteen qualitative and four quantitative variables were analyzed. The results showed that the main risk factors for high BMSCC were the presence of the pathogens S. aureus and S. agalactiae, non-use of pre and post-dipping, non-use of paper towel for drying teats, non-use herd monitoring by California Mastitis Tests (CMT) or individual SCC, absence of the milking line and the therapy of dry cows, and failures in hygiene of teats and udders before milking. Failures related to maintenance and use of milking equipment, including the application of manual pressure to milking assemblies, unregulated vacuum pressure in milking machine, loss of vacuum during milking, and maintenance failures of milking machine and bulk milk tank were also found as relevant risk factors. Moderate correlations were a lso observed between elevation of BMSCC and counts of S. aureus and S. agalactiae in the bulk milk tank and moderate correlation between counts of S. aureus and counts of S. agalactiae. Our results showed high levels of production losses and prevalence of S. aureus and S. agalactiae pathogens, with high scores of antimicrobial resistance in the studied herds. The risk factors for BMSCC ≥ 700,000 cells/mL of milk were related to failures in relation to the classic measures of control of bovine mastitis, emphasizing the importance of the same to improve milk quality and mitigate the losses caused by this disease to producers and industry.A mastite bovina é a doença mais importante em rebanhos leiteiros em nível mundial, acarretando grandes prejuízos sanitários e econômicos aos produtores e à indústria. Considerando o aspecto multifatorial da doença, o conhecimento dos agentes envolvidos e de seus perfis de suscetibilidade aos antimicrobianos, o impacto da mesma sobre a produção e os fatores de risco envolvidos em sua presença nos rebanhos, é de suma importância para o controle da doença. Todo o experimento foi realizado em parceria com um laticínio da região de Lavras/Sul de Minas Gerais que contava à época com 306 propriedades fornecedoras de leite. No primeiro estudo avaliou-se o impacto econômico da mastite sobre a produção de leite. Para se calcular as perdas de produção, utilizou-se as médias de três contagens de células somáticas do leite do tanque (CCSt), de 306 propriedades para se estimar as perdas de produção, através da equação de regressão linear y= - 4,7908 + 0,0226x, na qual a variável “x” representa a CCSt e “y” as perdas percentuais diárias. Foram observadas médias de CCSt de 482.650 cels/mL e de 43.710 UFC/mL de leite para a contagem bacteriana total (CBT). Entre as propriedades amostradas, verificou-se que 35,6% e 7,84% se encontravam acima dos limites estabelecidos pela legislação vigente para a CCSt e CBT, respectivamente. Os resultados demonstraram que as médias de perdas diárias na produção de leite devido a mastite foram de 6,27%. No segundo estudo, se avaliou a prevalência de Staphylococcus aureus e Streptococcus agalactiae, por meio de análises microbiológicas de amostras de leite provenientes dos tanques de expansão das 306 propriedades, e a sua relação com os índices de CCSt, CBT e produção diária, além de analisar a resistência destes patógenos aos antimicrobianos. Foi avaliada a suscetibilidade de isolados de S. aureus (99) e de S. agalactiae (93) a antimicrobianos por meio da técnica de difusão em discos. Nossos resultados apontaram altas prevalências dos patógenos S. aureus (70,3%) e S. agalactiae (67,0%) e associações entre a CCSt e a presença dos patógenos S. aureus e S. agalactiae, e também entre a CBT e a presença de S. agalactiae, demonstrando a interferência negativa destes patógenos nestes quesitos de qualidade do leite. Níveis elevados de resistência e de multirresistência foram observados para os patógenos S. aureus e S. agalactiae nos rebanhos estudados. No terceiro estudo, visando determinar os fatores de risco envolvidos com a CCSt elevada, foi realizado um estudo de caso -controle envolvendo 46 rebanhos bovinos, dos quais 30 foram considerados casos (CCSt ≥ 700.000 cels/mL de leite) e 16 como controles (CCSt ≤ 200.000 cels/mL de leite). Foram analisadas 16 variáveis qualitativas e 4 variáveis quantitativas. Os resultados demonstraram que os fatores de risco para altos valores de CCSt foram: presença dos patógenos S. aureus e S. agalactiae, não utilização do pré e de pósdipping, não utilização de papel toalha para secagem de tetos, não utilização de CMT ou CCS individual para monitoramento do rebanho, ausência da linha de ordenha e da terapia de vacas secas e falhas na higiene de tetos e de úbere antes da ordenha. Falhas inerentes à manutenção e utilização dos equipamentos de ordenha, incluindo a aplicação de pressão manual nos conjuntos da ordenha, pressão de vácuo da ordenha desregulada, perda de vácuo durante a ordenha e falhas de manutenção da ordenhadeira e do tanque de expansão. Também se observou correlações moderadas entre elevação da CCSt e as contagens de S. aureus e S. agalactiae no leite do tanque e correlação moderada entre contagens de S. aureus e contagens de S. agalactiae. Nossos resultados apontaram uma alta prevalência dos patógenos S. aureus e S. agalactiae, com altos índices de resistência aos antimicrobianos. Os fatores de risco para CCSt elevadas mais relevantes foram relacionados com falhas em relação às medidas clássicas de controle da mastite, salientando a importância das mesmas para a melhoria da qualidade do leite e mitigar os prejuízos ocasionados pela doença aos produtores e à indústria.Universidade Federal de LavrasPrograma de Pós-graduação em Ciências VeterináriasUFLAbrasilDepartamento de Medicina VeterináriaCosta, Geraldo Márcio daRocha, Christiane Maria Barcellos Magalhães daBruhn, Fábio Raphael PascotiSilva, Délcio Bueno daRocha, Christiane Maria Barcellos Magalhães daPinto, Sandra MariaMesquita, Alan Andrade2018-01-12T09:57:08Z2018-01-12T09:57:08Z2017-12-262017-11-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfMESQUITA, A. A. Impacto da Mastite bovina por Sthapylococcus aureus e Streptococcus agalactiae. 2017. 73 p. Tese (Doutorado em Ciências Veterinárias)-Universidade Federal de Lavras, Lavras, 2017.http://repositorio.ufla.br/jspui/handle/1/28358porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2018-07-24T13:52:11Zoai:localhost:1/28358Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2018-07-24T13:52:11Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Impacto da Mastite bovina por Sthapylococcus aureus e Streptococcus agalactiae
Impact of bovine Mastitis by Staphylococcus aureus and Streptococcus agalactiae
title Impacto da Mastite bovina por Sthapylococcus aureus e Streptococcus agalactiae
spellingShingle Impacto da Mastite bovina por Sthapylococcus aureus e Streptococcus agalactiae
Mesquita, Alan Andrade
Mastite bovina
Mastite - Custos
Mastite - Controle
Bovinos leiteiros - Doenças
Contagem bacteriana total
Bovine mastitis
Mastitis - Costs
Mastitis - Control
Dairy cattle - Diseases
Total bacterial count
Medicina Veterinária
title_short Impacto da Mastite bovina por Sthapylococcus aureus e Streptococcus agalactiae
title_full Impacto da Mastite bovina por Sthapylococcus aureus e Streptococcus agalactiae
title_fullStr Impacto da Mastite bovina por Sthapylococcus aureus e Streptococcus agalactiae
title_full_unstemmed Impacto da Mastite bovina por Sthapylococcus aureus e Streptococcus agalactiae
title_sort Impacto da Mastite bovina por Sthapylococcus aureus e Streptococcus agalactiae
author Mesquita, Alan Andrade
author_facet Mesquita, Alan Andrade
author_role author
dc.contributor.none.fl_str_mv Costa, Geraldo Márcio da
Rocha, Christiane Maria Barcellos Magalhães da
Bruhn, Fábio Raphael Pascoti
Silva, Délcio Bueno da
Rocha, Christiane Maria Barcellos Magalhães da
Pinto, Sandra Maria
dc.contributor.author.fl_str_mv Mesquita, Alan Andrade
dc.subject.por.fl_str_mv Mastite bovina
Mastite - Custos
Mastite - Controle
Bovinos leiteiros - Doenças
Contagem bacteriana total
Bovine mastitis
Mastitis - Costs
Mastitis - Control
Dairy cattle - Diseases
Total bacterial count
Medicina Veterinária
topic Mastite bovina
Mastite - Custos
Mastite - Controle
Bovinos leiteiros - Doenças
Contagem bacteriana total
Bovine mastitis
Mastitis - Costs
Mastitis - Control
Dairy cattle - Diseases
Total bacterial count
Medicina Veterinária
description Bovine mastitis is the most important disease in dairy herds worldwide, causing great health and economic losses to producers and industry, as well as having implications for public health due to the zoonotic potential. Considering the multifactorial aspect of this disease, the knowledge of the agents involved in its etiology, antimicrobial susceptibility profiles, its economic impact on the production and the risk factors involved in its occurrence has high importance for its control. The experiment was carried out in partnership with a dairy industry in the region of Lavras/South of Minas Gerais, which at that time had 306 milk supply properties. In the first study, the impact of bovine mastitis on milk production was evaluated. In order to calculate the production losses, the means of three BMSCC of the 306 properties, obtained at intervals of 15 days between the collections, were used, and losses were estimated through the linear regression equation y = -4.7908 + 0.0226x, where the variable "x" represents the BMSCC and “y” the estimates of milk percentage losses. The mean of BMSCC between the studied properties was 482,650 cells/mL, and 43,710 CFU/mL of milk for total bacterial counts (TBC), with 35.6% and 7.84% of the farms above of limits established by current legislation for BMSCC and TBC, respectively. The results showed that the average daily losses in production due to mastitis were 6.27%. In the second study, the prevalence of Staphylococcus aureus and Streptococcus agalactiae was evaluated, by means of microbiological analyzes of milk samples collected from bulk milk tanks, and their relation with the BMSCC, TBC and daily production. Additionally, the resistance profile of S. aureus (99) and S. agalactiae (93) strains to antimicrobials was evaluated by disc diffusion technique. Our results showed high prevalences of S. aureus (70.3%) and S. agalactiae pathogens (67.0%), and associations between BMSCC and the presence of S. aureus and S. agalactiae pathogens and between the TBC and the presence of S. agalactiae, demonstrating the negative interference of these pathogens in these milk quality aspects. High levels of resistance and multiresistance were observed for S. aureus and S. agalactiae in the st udied herds. In the third study, a case-control study was carried out involving 46 dairy farms, of which 30 were considered as cases (CCSt ≥ 700,000 cells/mL of milk) and 16 as controls (CCSt ≤ 200,000 cells/mL of milk). Sixteen qualitative and four quantitative variables were analyzed. The results showed that the main risk factors for high BMSCC were the presence of the pathogens S. aureus and S. agalactiae, non-use of pre and post-dipping, non-use of paper towel for drying teats, non-use herd monitoring by California Mastitis Tests (CMT) or individual SCC, absence of the milking line and the therapy of dry cows, and failures in hygiene of teats and udders before milking. Failures related to maintenance and use of milking equipment, including the application of manual pressure to milking assemblies, unregulated vacuum pressure in milking machine, loss of vacuum during milking, and maintenance failures of milking machine and bulk milk tank were also found as relevant risk factors. Moderate correlations were a lso observed between elevation of BMSCC and counts of S. aureus and S. agalactiae in the bulk milk tank and moderate correlation between counts of S. aureus and counts of S. agalactiae. Our results showed high levels of production losses and prevalence of S. aureus and S. agalactiae pathogens, with high scores of antimicrobial resistance in the studied herds. The risk factors for BMSCC ≥ 700,000 cells/mL of milk were related to failures in relation to the classic measures of control of bovine mastitis, emphasizing the importance of the same to improve milk quality and mitigate the losses caused by this disease to producers and industry.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-26
2017-11-14
2018-01-12T09:57:08Z
2018-01-12T09:57:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv MESQUITA, A. A. Impacto da Mastite bovina por Sthapylococcus aureus e Streptococcus agalactiae. 2017. 73 p. Tese (Doutorado em Ciências Veterinárias)-Universidade Federal de Lavras, Lavras, 2017.
http://repositorio.ufla.br/jspui/handle/1/28358
identifier_str_mv MESQUITA, A. A. Impacto da Mastite bovina por Sthapylococcus aureus e Streptococcus agalactiae. 2017. 73 p. Tese (Doutorado em Ciências Veterinárias)-Universidade Federal de Lavras, Lavras, 2017.
url http://repositorio.ufla.br/jspui/handle/1/28358
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Lavras
Programa de Pós-graduação em Ciências Veterinárias
UFLA
brasil
Departamento de Medicina Veterinária
publisher.none.fl_str_mv Universidade Federal de Lavras
Programa de Pós-graduação em Ciências Veterinárias
UFLA
brasil
Departamento de Medicina Veterinária
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1815438969391808512