Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus)

Detalhes bibliográficos
Autor(a) principal: Botta, P.
Data de Publicação: 2010
Outros Autores: Sciara, A., Arranz, S., Murgas, L. D. S., Pereira, G. J. M., Oberlender, G.
Tipo de documento: Artigo
Idioma: spa
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/39163
Resumo: Currently, the storage of fish spermatozoa through cryopreservation is widely used. Although it is of common knowledge that the process of freezing / thawing generates DNA fragmentation of spermatozoa, the consequences of this process on embryonary development are unknown. There is great interest in developing methods for in vitro fertilization for the species Prochilodus lineatus using cryopreserved semen. In this paper we study the embryonic development of this fish, to lay the groundwork for the observation of abnormalities in the development of embryos derived from criopreserved spermatozoa. The eggs produced by this species are translucent and have a large perivitelline space, are of the telolecitic type, and presented meroblastic division during the early stages of development. Although the embrionary development of Prochilodus lineatus (Sábalo) took place in less time compared with Danio rerio (Zebrafish) the embryo goes through very similar stages. During the study it was possible to observe the periods of zygote, cleavage, blastula, gastrula, segmentation and hatching of the larvae of Sábalo. The different stages of development were successfully recorded, especially those after the transition of blastula media (TBM), when the embryo begins to transcribe its own genome.
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spelling Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus)Study of the embrionary development in sábalo (Prochilodus lineatus)Prochilodus lineatusPeces - EmbrionesFish - EmbryosPeixes - ReproduçãoDesenvolvimento embrionárioCurrently, the storage of fish spermatozoa through cryopreservation is widely used. Although it is of common knowledge that the process of freezing / thawing generates DNA fragmentation of spermatozoa, the consequences of this process on embryonary development are unknown. There is great interest in developing methods for in vitro fertilization for the species Prochilodus lineatus using cryopreserved semen. In this paper we study the embryonic development of this fish, to lay the groundwork for the observation of abnormalities in the development of embryos derived from criopreserved spermatozoa. The eggs produced by this species are translucent and have a large perivitelline space, are of the telolecitic type, and presented meroblastic division during the early stages of development. Although the embrionary development of Prochilodus lineatus (Sábalo) took place in less time compared with Danio rerio (Zebrafish) the embryo goes through very similar stages. During the study it was possible to observe the periods of zygote, cleavage, blastula, gastrula, segmentation and hatching of the larvae of Sábalo. The different stages of development were successfully recorded, especially those after the transition of blastula media (TBM), when the embryo begins to transcribe its own genome.Resumen: El almacenamiento de espermatozoides de peces por medio de la criopreservación es muy utilizado hoy en día. Si bien se sabe que el proceso de congelamiento/descongelamiento genera la fragmentación del ADN del espermatozoide, no se conocen las consecuencias que esto tiene en el desarrollo embrionario. Actualmente hay un gran interés en el desarrollo de métodos de fecundación in vitro para la especie Prochilodus lineatus utilizando semen criopreservado. En este trabajo estudiamos el desarrollo embrionario de esta especie, a fin de sentar las bases para la observación de anormalidades en el desarrollo de embriones obtenidos a partir de espermatozoides crioconservados. Los huevos producidos por esta especie son translúcidos y poseen un gran espacio perivitelino; son del tipo telolecítico y presentan una división meroblástica durante los primeros estadíos del desarrollo. Si bien el desarrollo embrionario de Prochilodus lineatus (sábalo) se llevó a cabo en menor tiempo en comparación con Danio rerio, las etapas que atravesó el embrión son muy similares. Durante el estudio se observaron los períodos de Cigoto, Clivaje, Blástula, Gástrula, Segmentación y Eclosión de la larva de sábalo. De esta manera se logró registrar los diferentes estadíos de su desarrollo, principalmente aquellos posteriores a la transición de la blástula media (TBM), a partir del cual el embrión comienza a transcribir su propio genoma.Universidad Austral de Chile2020-03-05T14:32:15Z2020-03-05T14:32:15Z2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBOTTA, P. et al. Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus). Archivos de Medicina Veterinária, Valdivia, v. 42, n. 2, p. 109-114, 2010.http://repositorio.ufla.br/jspui/handle/1/39163Archivos de Medicina Veterináriareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessBotta, P.Sciara, A.Arranz, S.Murgas, L. D. S.Pereira, G. J. M.Oberlender, G.spa2020-03-05T14:32:35Zoai:localhost:1/39163Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-03-05T14:32:35Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus)
Study of the embrionary development in sábalo (Prochilodus lineatus)
title Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus)
spellingShingle Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus)
Botta, P.
Prochilodus lineatus
Peces - Embriones
Fish - Embryos
Peixes - Reprodução
Desenvolvimento embrionário
title_short Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus)
title_full Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus)
title_fullStr Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus)
title_full_unstemmed Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus)
title_sort Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus)
author Botta, P.
author_facet Botta, P.
Sciara, A.
Arranz, S.
Murgas, L. D. S.
Pereira, G. J. M.
Oberlender, G.
author_role author
author2 Sciara, A.
Arranz, S.
Murgas, L. D. S.
Pereira, G. J. M.
Oberlender, G.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Botta, P.
Sciara, A.
Arranz, S.
Murgas, L. D. S.
Pereira, G. J. M.
Oberlender, G.
dc.subject.por.fl_str_mv Prochilodus lineatus
Peces - Embriones
Fish - Embryos
Peixes - Reprodução
Desenvolvimento embrionário
topic Prochilodus lineatus
Peces - Embriones
Fish - Embryos
Peixes - Reprodução
Desenvolvimento embrionário
description Currently, the storage of fish spermatozoa through cryopreservation is widely used. Although it is of common knowledge that the process of freezing / thawing generates DNA fragmentation of spermatozoa, the consequences of this process on embryonary development are unknown. There is great interest in developing methods for in vitro fertilization for the species Prochilodus lineatus using cryopreserved semen. In this paper we study the embryonic development of this fish, to lay the groundwork for the observation of abnormalities in the development of embryos derived from criopreserved spermatozoa. The eggs produced by this species are translucent and have a large perivitelline space, are of the telolecitic type, and presented meroblastic division during the early stages of development. Although the embrionary development of Prochilodus lineatus (Sábalo) took place in less time compared with Danio rerio (Zebrafish) the embryo goes through very similar stages. During the study it was possible to observe the periods of zygote, cleavage, blastula, gastrula, segmentation and hatching of the larvae of Sábalo. The different stages of development were successfully recorded, especially those after the transition of blastula media (TBM), when the embryo begins to transcribe its own genome.
publishDate 2010
dc.date.none.fl_str_mv 2010
2020-03-05T14:32:15Z
2020-03-05T14:32:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv BOTTA, P. et al. Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus). Archivos de Medicina Veterinária, Valdivia, v. 42, n. 2, p. 109-114, 2010.
http://repositorio.ufla.br/jspui/handle/1/39163
identifier_str_mv BOTTA, P. et al. Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus). Archivos de Medicina Veterinária, Valdivia, v. 42, n. 2, p. 109-114, 2010.
url http://repositorio.ufla.br/jspui/handle/1/39163
dc.language.iso.fl_str_mv spa
language spa
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Austral de Chile
publisher.none.fl_str_mv Universidad Austral de Chile
dc.source.none.fl_str_mv Archivos de Medicina Veterinária
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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