Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus)
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | spa |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/39163 |
Resumo: | Currently, the storage of fish spermatozoa through cryopreservation is widely used. Although it is of common knowledge that the process of freezing / thawing generates DNA fragmentation of spermatozoa, the consequences of this process on embryonary development are unknown. There is great interest in developing methods for in vitro fertilization for the species Prochilodus lineatus using cryopreserved semen. In this paper we study the embryonic development of this fish, to lay the groundwork for the observation of abnormalities in the development of embryos derived from criopreserved spermatozoa. The eggs produced by this species are translucent and have a large perivitelline space, are of the telolecitic type, and presented meroblastic division during the early stages of development. Although the embrionary development of Prochilodus lineatus (Sábalo) took place in less time compared with Danio rerio (Zebrafish) the embryo goes through very similar stages. During the study it was possible to observe the periods of zygote, cleavage, blastula, gastrula, segmentation and hatching of the larvae of Sábalo. The different stages of development were successfully recorded, especially those after the transition of blastula media (TBM), when the embryo begins to transcribe its own genome. |
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Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus)Study of the embrionary development in sábalo (Prochilodus lineatus)Prochilodus lineatusPeces - EmbrionesFish - EmbryosPeixes - ReproduçãoDesenvolvimento embrionárioCurrently, the storage of fish spermatozoa through cryopreservation is widely used. Although it is of common knowledge that the process of freezing / thawing generates DNA fragmentation of spermatozoa, the consequences of this process on embryonary development are unknown. There is great interest in developing methods for in vitro fertilization for the species Prochilodus lineatus using cryopreserved semen. In this paper we study the embryonic development of this fish, to lay the groundwork for the observation of abnormalities in the development of embryos derived from criopreserved spermatozoa. The eggs produced by this species are translucent and have a large perivitelline space, are of the telolecitic type, and presented meroblastic division during the early stages of development. Although the embrionary development of Prochilodus lineatus (Sábalo) took place in less time compared with Danio rerio (Zebrafish) the embryo goes through very similar stages. During the study it was possible to observe the periods of zygote, cleavage, blastula, gastrula, segmentation and hatching of the larvae of Sábalo. The different stages of development were successfully recorded, especially those after the transition of blastula media (TBM), when the embryo begins to transcribe its own genome.Resumen: El almacenamiento de espermatozoides de peces por medio de la criopreservación es muy utilizado hoy en día. Si bien se sabe que el proceso de congelamiento/descongelamiento genera la fragmentación del ADN del espermatozoide, no se conocen las consecuencias que esto tiene en el desarrollo embrionario. Actualmente hay un gran interés en el desarrollo de métodos de fecundación in vitro para la especie Prochilodus lineatus utilizando semen criopreservado. En este trabajo estudiamos el desarrollo embrionario de esta especie, a fin de sentar las bases para la observación de anormalidades en el desarrollo de embriones obtenidos a partir de espermatozoides crioconservados. Los huevos producidos por esta especie son translúcidos y poseen un gran espacio perivitelino; son del tipo telolecítico y presentan una división meroblástica durante los primeros estadíos del desarrollo. Si bien el desarrollo embrionario de Prochilodus lineatus (sábalo) se llevó a cabo en menor tiempo en comparación con Danio rerio, las etapas que atravesó el embrión son muy similares. Durante el estudio se observaron los períodos de Cigoto, Clivaje, Blástula, Gástrula, Segmentación y Eclosión de la larva de sábalo. De esta manera se logró registrar los diferentes estadíos de su desarrollo, principalmente aquellos posteriores a la transición de la blástula media (TBM), a partir del cual el embrión comienza a transcribir su propio genoma.Universidad Austral de Chile2020-03-05T14:32:15Z2020-03-05T14:32:15Z2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBOTTA, P. et al. Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus). Archivos de Medicina Veterinária, Valdivia, v. 42, n. 2, p. 109-114, 2010.http://repositorio.ufla.br/jspui/handle/1/39163Archivos de Medicina Veterináriareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessBotta, P.Sciara, A.Arranz, S.Murgas, L. D. S.Pereira, G. J. M.Oberlender, G.spa2020-03-05T14:32:35Zoai:localhost:1/39163Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-03-05T14:32:35Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus) Study of the embrionary development in sábalo (Prochilodus lineatus) |
title |
Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus) |
spellingShingle |
Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus) Botta, P. Prochilodus lineatus Peces - Embriones Fish - Embryos Peixes - Reprodução Desenvolvimento embrionário |
title_short |
Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus) |
title_full |
Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus) |
title_fullStr |
Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus) |
title_full_unstemmed |
Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus) |
title_sort |
Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus) |
author |
Botta, P. |
author_facet |
Botta, P. Sciara, A. Arranz, S. Murgas, L. D. S. Pereira, G. J. M. Oberlender, G. |
author_role |
author |
author2 |
Sciara, A. Arranz, S. Murgas, L. D. S. Pereira, G. J. M. Oberlender, G. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Botta, P. Sciara, A. Arranz, S. Murgas, L. D. S. Pereira, G. J. M. Oberlender, G. |
dc.subject.por.fl_str_mv |
Prochilodus lineatus Peces - Embriones Fish - Embryos Peixes - Reprodução Desenvolvimento embrionário |
topic |
Prochilodus lineatus Peces - Embriones Fish - Embryos Peixes - Reprodução Desenvolvimento embrionário |
description |
Currently, the storage of fish spermatozoa through cryopreservation is widely used. Although it is of common knowledge that the process of freezing / thawing generates DNA fragmentation of spermatozoa, the consequences of this process on embryonary development are unknown. There is great interest in developing methods for in vitro fertilization for the species Prochilodus lineatus using cryopreserved semen. In this paper we study the embryonic development of this fish, to lay the groundwork for the observation of abnormalities in the development of embryos derived from criopreserved spermatozoa. The eggs produced by this species are translucent and have a large perivitelline space, are of the telolecitic type, and presented meroblastic division during the early stages of development. Although the embrionary development of Prochilodus lineatus (Sábalo) took place in less time compared with Danio rerio (Zebrafish) the embryo goes through very similar stages. During the study it was possible to observe the periods of zygote, cleavage, blastula, gastrula, segmentation and hatching of the larvae of Sábalo. The different stages of development were successfully recorded, especially those after the transition of blastula media (TBM), when the embryo begins to transcribe its own genome. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2020-03-05T14:32:15Z 2020-03-05T14:32:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
BOTTA, P. et al. Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus). Archivos de Medicina Veterinária, Valdivia, v. 42, n. 2, p. 109-114, 2010. http://repositorio.ufla.br/jspui/handle/1/39163 |
identifier_str_mv |
BOTTA, P. et al. Estudio del desarrollo embrionario del sábalo (Prochilodus lineatus). Archivos de Medicina Veterinária, Valdivia, v. 42, n. 2, p. 109-114, 2010. |
url |
http://repositorio.ufla.br/jspui/handle/1/39163 |
dc.language.iso.fl_str_mv |
spa |
language |
spa |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidad Austral de Chile |
publisher.none.fl_str_mv |
Universidad Austral de Chile |
dc.source.none.fl_str_mv |
Archivos de Medicina Veterinária reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1815439320257921024 |